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191.
以植物钾离子外排通道(K’channeloutward.rectifier,KCO)基因为研究对象,运用CodonW软件分析了75个植物KCO基因密码子的使用模式,探讨密码子的使用模式和影响密码子使用的各种可能因素。结果表明:碱基组成差异(r=0.961,P〈0.01)和自然选择(r=0.568,P〈0.01)是影响密码子使用的主要因素,并且高表达的基因强烈偏爱使用以G或C结尾的密码子。确定了UUC、CUC等26个均以G/C结尾的密码子为植物KcD基因的高表达优越密码子。 相似文献
192.
钙磷是蜜蜂饲粮中必需的常量元素。为了探讨蜜蜂人工代用花粉中适宜的钙磷水平,本试验选取蜂王、群势基本一致的意大利蜜蜂Apis mellifera ligustica Spinola 70群,随机分为14个组,每组5群蜂,前12组饲喂采用均匀设计法配制的不同钙磷水平的人工代用花粉。第13组饲喂不加钙磷的人工代用花粉为负对照,第14组饲喂纯油菜花粉为正对照。试验从春繁开始,到刺槐流蜜期结束为止。试验期间测定蜂群的采食量、蜂群群势、幼蜂初生重,成蜂蜂体组织内钙磷含量。结果表明:当人工代用花粉中钙磷水平分别为0,0.65%时,蜂群的采食量、蜂群群势、幼蜂初生重均取得最大值;成蜂蜂体组织内钙含量与人工代用花粉中钙磷含量成正相关,成蜂蜂体组织内磷含量与人工代用花粉中钙磷含量之间没有相关性。 相似文献
193.
The superfamily Cobitoidea of the order Cypriniformes is a diverse group of fishes, inhabiting freshwater ecosystems across Eurasia and North Africa. The phylogenetic relationships of this well-corroborated natural group and diverse clade are critical to not only informing scientific communities of the phylogeny of the order Cypriniformes, the world's largest freshwater fish order, but are key to every area of comparative biology examining the evolution of traits, functional structures, and breeding behaviors to their biogeographic histories, speciation, anagenetic divergence, and divergence time estimates. In the present study, two mitochondrial gene sequences (COI, ND4+5) and four single-copy nuclear gene segments (RH1, RAG1, EGR2B, IRBP) were used to infer the phylogenetic relationships of the Cobitoidea as reconstructed from maximum likelihood (ML) and partitioned Bayesian Analysis (BA). Analyses of the combined mitochondrial/nuclear gene datasets revealed five strongly supported monophyletic Cobitoidea families and their sister-group relationships: Botiidae+(Vaillantellidae+(Cobitidae+(Nemacheilidae+Balitoridae))). These recovered relationships are in agreement with previous systematic studies on the order Cypriniformes and/or those focusing on the superfamily Cobitoidea. Using these relationships, our analyses revealed pattern lineage- or ecological-group-specific evolution of these genes for the Cobitoidea. These observations and results corroborate the hypothesis that these group-specific-ancestral ecological characters have contributed in the diversification and/or adaptations within these groups. Positive selections were detected in RH1 of nemacheilids and in RAG1 of nemacheilids and genus Vaillantella, which indicated that evolution of RH1 (related to eye's optic sense) and RAG1 (related to immunity) genes appeared to be important for the diversification of these groups. The balitorid lineage (those species inhabiting fast-flowing riverine habitats) had, as compared with other cobitoid lineages, significantly different dN/dS, dN and dS values for ND4 and IRBP genes. These significant differences are usually indicative of weaker selection pressure, and lineage-specific evolution on genes along the balitorid lineage. Furthermore, within Cobitoidea, excluding balitorids, species living in subtropics had significantly higher dN/dS values in RAG1 and IRBP genes than those living in temperate and tropical zones. Among tropical cobitoids, genes COI, ND5, EGR2B, IRBP and RH1, had a significantly higher mean dS value than those species in subtropical and temperate groups. These findings suggest that the evolution of these genes could also be ecological-group-specific and may have played an important role in the adaptive evolution and diversification of these groups. Thus, we hypothesize that the genes included in the present study were actively involved in lineage- and/or ecological-group-specific evolutionary processes of the highly diverse Cobitoidea. These two evolutionary patterns, both subject to further testing, are hypothesized as integral in the diversification with this major clade of the world's most diverse group of freshwater fishes. 相似文献
194.
Bonnie KE Milstein MS Calcutt SE Ross SR Wagner KE Lonsdorf EV 《American journal of primatology》2012,74(7):661-668
As a result of environmental variability, animals may be confronted with uncertainty surrounding the presence of, or accessibility to, food resources at a given location or time. While individuals can rely on personal experience to manage this variability, the behavior of members of an individual's social group can also provide information regarding the availability or location of a food resource. The purpose of the present study was to measure how captive chimpanzees individually and collectively adjust their foraging strategies at an artificial termite mound, as the availability of resources provided by the mound varied over a number of weeks. As predicted, fishing activity at the mound was related to resource availability. However, chimpanzees continued to fish at unbaited locations on the days and weeks after a location had last contained food. Consistent with previous studies, our findings show that chimpanzees do not completely abandon previously learned habits despite learning individually and/or socially that the habit is no longer effective. 相似文献
195.
Order Chiroptera is a unique group of mammals whose members have attained self-powered flight as their main mode of locomotion. Much speculation persists regarding bat evolution; however, lack of sufficient molecular data hampers evolutionary and conservation studies. Of ~ 1200 species, complete mitochondrial genome sequences are available for only eleven. Additional sequences should be generated if we are to resolve many questions concerning these fascinating mammals. Herein, we describe the complete mitochondrial genomes of three bats: Corynorhinus rafinesquii, Lasiurus borealis and Artibeus lituratus. We also compare the currently available mitochondrial genomes and analyze codon usage in Chiroptera. C. rafinesquii, L. borealis and A. lituratus mitochondrial genomes are 16438 bp, 17048 bp and 16709 bp, respectively. Genome organization and gene arrangements are similar to other bats. Phylogenetic analyses using complete mitochondrial genome sequences support previously established phylogenetic relationships and suggest utility in future studies focusing on the evolutionary aspects of these species. Comprehensive analyses of available bat mitochondrial genomes reveal distinct nucleotide patterns and synonymous codon preferences corresponding to different chiropteran families. These patterns suggest that mutational and selection forces are acting to different extents within Chiroptera and shape their mitochondrial genomes. 相似文献
196.
Random effects models are widely used in population pharmacokinetics and dose-finding studies. However, when more than one observation is taken per patient, the presence of correlated observations (due to shared random effects and possibly residual serial correlation) usually makes the explicit determination of optimal designs difficult. In this article, we introduce a class of multiplicative algorithms to be able to handle correlated data and thus allow numerical calculation of optimal experimental designs in such situations. In particular, we demonstrate its application in a concrete example of a crossover dose-finding trial, as well as in a typical population pharmacokinetics example. Additionally, we derive a lower bound for the efficiency of any given design in this context, which allows us on the one hand to monitor the progress of the algorithm, and on the other hand to investigate the efficiency of a given design without knowing the optimal one. Finally, we extend the methodology such that it can be used to determine optimal designs if there exist some requirements regarding the minimal number of treatments for several (in some cases all) experimental conditions. 相似文献
197.
Daniel J. D. Natusch Jessica A. Lyons 《Biological journal of the Linnean Society. Linnean Society of London》2012,107(2):269-276
We describe a 15‐year study of the loss of reproductive fitness and population decline in Adenostoma sparsifolium, a rosaceous shrub endemic in the fire‐prone chaparral vegetation of southern California (USA) and adjacent northern Baja California, Mexico. Our studies of background extinction concentrated on small relict populations occurring in the eastern Santa Monica Mountains where reproduction is genetically compromised by uniquely high rates of embryonic/endosperm abortion (97–99%) resulting largely from self‐pollination in highly heterozygous populations. Environmental factors further reduce reproductive fitness. The relatively few viable seeds produced are not well adapted to survive wildfires that are a regular (approximately 21 years) occurrence in chaparral. Seedling recruitment after burning is rare and any established seedlings ultimately die from the annual 4–9‐month summer droughts typical of Mediterranean climates. Adult mortality is manifest from wildfire (approximately 6%) and occasional multiple‐year droughts (approximately 15%). Given the virtual absence of new post‐fire reproduction and a low but persistent rate of adult mortality, slow population demise resulting in background extinction is inevitable. We posit that A. sparsifolium is ecologically ‘out of place’ in the present chaparral environment and appears best adapted to a moister climate with summer rains and few wildfires that prevailed before the increasing aridity and warming from mid‐Holocene to the present. © 2011 The Linnean Society of London, Biological Journal of the Linnean Society, 2012, 105 , 269–292. 相似文献
198.
Polshakov VI Eliseev BD Birdsall B Frolova LY 《Protein science : a publication of the Protein Society》2012,21(6):896-903
The high-resolution NMR structure of the N-domain of human eRF1, responsible for stop codon recognition, has been determined in solution. The overall fold of the protein is the same as that found in the crystal structure. However, the structures of several loops, including those participating in stop codon decoding, are different. Analysis of the NMR relaxation data reveals that most of the regions with the highest structural discrepancy between the solution and solid states undergo internal motions on the ps-ns and ms time scales. The NMR data show that the N-domain of human eRF1 exists in two conformational states. The distribution of the residues having the largest chemical shift differences between the two forms indicates that helices α2 and α3, with the NIKS loop between them, can switch their orientation relative to the β-core of the protein. Such structural plasticity may be essential for stop codon recognition by human eRF1. 相似文献
199.
Huang Y O'Mara B Conover M Ludwig R Fu J Tao L Li ZJ Rieble S Grace MJ Russell RJ 《Protein science : a publication of the Protein Society》2012,21(5):625-632
A novel amino acid misincorporation, in which the intended glycine (Gly) residues were replaced by a glutamic acid (Glu), was observed in a recombinant protein expressed by Escherichia coli. The misincorporation was identified by peptide mapping and liquid chromatography-tandem mass spectrometric analysis on proteolyzed peptides of the protein and verified using the corresponding synthetic peptides containing the misincorporated residues. Analysis of the distribution of the misincorporated residues and their codon usage shows strong correlation between this misincorporation and the use of rarely used codon within the E. coli expression system. Results in this study suggest that the usage of the rare codon GGA has resulted in a Glu for Gly misincorporation. 相似文献
200.
Microbes are known for their unique ability to adapt to varying lifestyle and environment, even to the extreme or adverse ones. The genomic architecture of a microbe may bear the signatures not only of its phylogenetic position, but also of the kind of lifestyle to which it is adapted. The present review aims to provide an account of the specific genome signatures observed in microbes acclimatized to distinct lifestyles or ecological niches. Niche-specific signatures identified at different levels of microbial genome organization like base composition, GC-skew, purine-pyrimidine ratio, dinucleotide abundance, codon bias, oligonucleotide composition etc. have been discussed. Among the specific cases highlighted in the review are the phenomena of genome shrinkage in obligatory host-restricted microbes, genome expansion in strictly intra-amoebal pathogens, strand-specific codon usage in intracellular species, acquisition of genome islands in pathogenic or symbiotic organisms, discriminatory genomic traits of marine microbes with distinct trophic strategies, and conspicuous sequence features of certain extremophiles like those adapted to high temperature or high salinity. 相似文献