Bacterial Diversity in Linglong Gold Mine,China

Bacteria have been actively regulating cycles of various elements in the environment. To explore the potential bacterial role in gold biogeochemical cycling, this study analyzed the bacterial diversity of mine rock (MR) and surface soil (SS) samples from Linglong gold mine using 16S rRNA gene clone library analysis and cultivation method. From MR, 24 operational taxonomic units (OTUs) were identified from MR, covering 3 phyla and 18 genera. Meanwhile, 24 OTUs were identified from SS, including 4 phyla and 18 genera. Compared with 16S rRNA gene clone library analysis, 28 aerobic and 34 anaerobic isolates were obtained, whereas 26 aerobic and 71 anaerobic strains were isolated from SS. The cultivable bacteria were affiliated with Firmicutes, Proteobacteria and Actinobacteria phyla, and dominated by Firmicutes. These results underscore the high level of bacterial diversity in the gold mine. Our study provides information on the microbial diversity in Linglong gold mine and sheds light on the existence and potential function of bacteria in the gold biogeochemical cycling.     

Effects of phosphate-solubilizing bacteria application on soil phosphorus availability in coal mining subsidence area in Shanxi

The effect of phosphate-solubilizing bacteria (PSB) application on phosphorus (P) availability in reclaimed soil in coal mining subsidence region was investigated. Seven treatments were carried out including control, chicken manure (CM), PSB, PSB + tricalcium phosphate (TCP), CM?+?TCP, PSB?+?ground phosphate rock (GPR) and CM?+?GPR. The results showed soil Olsen-P concentration and phosphatase level as well as the yield of pakchoi (Brassica chinensis L.) were significantly higher in PSB application treatments compared to the corresponding CM application treatments. Soil phosphatase, invertase and urease contents were increased most significantly in PSB treatment, 1.18-, 1.31- and 2.32-fold higher than those in the control, respectively. Soil Ca₂-P, Ca₈-P, Fe-P and Al-P concentrations exhibited the greatest increases in PSB?+?TCP treatment, while occluded-P showed minor changes in different treatments. Application of PSB fertilizer reduced the transformation of Olsen-P to Ca₁₀-P, thus increasing P availability in reclaimed soil of coal mining subsidence area.     

Microdynamics and seasonal changes in manganese oxide epiprecipitation in Pinal Creek, Arizona

In Pinal Creek, Arizona, Mn oxyhydroxides (MnO_x) collect as thick precipitates on surface sediment, within the streambed, beneath algal mats, and on submerged and emergent plants and mosses. The proximate source of Mn is a thick, alluvial alkaline aquifer that was contaminated by past acid mine waste disposal practices associated with copper mines located upstream in the Globe–Miami area. Almost every organism in Pinal Creek is coated with MnO_x. Some are actively precipitating manganese, and others are doing it passively. The variety and seasonality of epilithic biological processes resulting in Mn oxidation (epiprecipitation) was studied for more than a year by analyzing artificial substrates placed in surface water having different flows and different vegetation types and densities. Most epiprecipitation took place on the holdfasts of the green alga, Ulothrix sp., and the iron bacterium, Leptothrix discophora. Extensive patches of MnO_x also coated extracellular polymeric substances of fungal hyphae and bacterial filaments. The dominant macroscopic precipitation was in the form of MnO_x clumps on mosses, green algae, and cyanobacterial mats, consistent with precipitation by pH elevation during photosynthesis. Most oxidation occurred in the spring and summer, in agreement with thermal, biological, and chemical activity models. More biological oxidation occurred in swifter water, consistent with oxygen elevation models. The efficiency of this naturally occurring, diverse ecosystem suggests that remediation efforts to remove metal contaminants such as Mn should focus on creation of habitats that raise biodiversity.US Geological Survey, retired     

Spontaneous Vegetation on Overburden Piles in the Coal Basin of Santa Catarina,Brazil

The objective of this work was to select indigenous vegetal species for restoration programs aiming at the regeneration of ombrophilous dense forest. Thirty‐five spoil piles located in the county of Sideropolis, Santa Catarina, that received overburden disposal for 39 years (1950–1989) were selected for study because they exhibited remarkable spontaneous regrowth of trees compared to surrounding spoil piles. Floristic inventory covered the whole area of the 35 piles, whereas survey on phytosociology and natural regeneration studies were conducted in 70 plots distributed along the 35 piles. Floristic inventory recorded 83 species from 28 botanical families. Herbaceous terricolous plants constituted the predominant species (47.0%), followed by shrubs (26.5%), trees (19.3%), and vines (7.2%). Results from surveys on phytosociology and natural regeneration, focused on shrubs and trees, recorded incipient ecological succession. In addition, the most adapted species recorded on the overburden piles, as ranked by index of natural regeneration (RNT) plus importance value index (IVI), were as follows: Clethra scabra (RNT = 23.93%; IVI = 17.28%), Myrsine coriacea (RNT = 20.93%, IVI = 11.26%), Eupatorium intermedium (RNT = 7.56%, IVI = 0.40%), Miconia ligustroides (RNT = 5.84%, IVI = 2.37%), Ossaea amygdaloides (RNT = 3.84%, IVI = 1.30%), Tibouchina sellowiana (RNT = 3.29%, IVI = 1.94%), Eup. inulaefolium (RNT = 2.65%, IVI = 0.80%), and Baccharis dracunculifolia (RNT = 2.28%; IVI = 0.56%). High values of IVI and RNT exhibited by the exotic species Eucalyptus saligna (IVI = 21.73%, RNT = 51.41%) indicated strong competition between exotic and indigenous species. Severe chemical (acidic pH and lack of nutrients) and physical (coarse substrate and slope angle of 40–50°) characteristics displayed by the overburden piles constituted limitations to floristic diversity and size of indigenous trees, indicating the need for substrate reclamation prior to forest restoration.     

Screening of Australian Native Grasses for Rhizoremediation of Aliphatic Hydrocarbon-Contaminated Soil

Rhizoremediation involves the breakdown of contaminants in soil resulting from microbial activity that is enhanced in the plant root zone. The objective of this study was to identify Australian native grass species as suitable candidates for rhizoremediation application. Seeds of nine perennial Australian native grasses were sown in soil from a mine site and artificially contaminated with a 60:40 diesel/oil mixture at concentrations of 1% (w/w), 0.5% (w/w), and 0% (control). Seedling emergence was not adversely affected by the presence of hydrocarbon contamination for all but one grass species. Three promising species (Brachiaria decumbens, Cymbopogon ambiguus, and Microlaena stipoides var. Griffin) were assessed for growth characterization in contaminated and uncontaminated soils. The evaluated species survived for 120 days in the contaminated soil and, in some instances, produced considerably more root biomass in the presence of contamination. C. ambiguus showed growth stimulation in the presence of contamination (1% and 0.5% w/w) with significantly increased root biomass production compared with the control (p = 0.0001). B. decumbens and M. stipoides showed tolerance, without adverse growth effects in the presence of diesel/oil at the exposed concentrations. Stimulation of the rhizosphere microbial population that is capable of degrading diesel/oil was found for all of the species tested, using a most probable number method for enumeration. This investigation has identified suitable candidates for further investigation of their rhizoremediation potential.     

Comparison of Organic and Chemical Soil Amendments Used in the Reforestation of a Harsh Sierra Nevada Site

Abstract A comparison of a composted organic amendment, a controlled‐release fertilizer, and induced mycorrhizal inoculation as affecting the establishment and nutrition of bareroot Jeffrey pine (Pinus jeffreyi Grev. & Balf.) was conducted on a Sierra Nevada surface mine. The soil amendments were applied at outplanting to the backfill of augered planting holes, with a low rate of 8 g and a high rate of 16 g per seedling for the fertilizer, Gromax 21‐6‐2 + Minors, whereas a single rate of 2.0 L was used for organic matter. Colonization by Pisolithus tinctorius (Pers.) Coker & Couch was induced by coating the root systems with basidiospores suspended in a gel carrier. The organic amendment especially, but also mycorrhizal inoculation, caused substantial seedling mortality, whereas survival was unaffected by controlled‐release fertilization. Gromax applied at the high rate produced a 74% increase in shoot volume after three growing seasons, whereas the organic amendment reduced volume by 28%. Growth was unaffected by mycorrhizal treatment. The growth response to the 16‐g Gromax application probably reflected enhanced N, P, and K nutrition and decreased concentrations of potentially toxic metallic elements, including Mn and Al among others, as revealed through foliar analysis. Because they were accompanied by growth reduction, nutritional responses to the organic amendment, which involved both macronutrients and trace elements, were of little consequence. Impaired water relations may account for the poor response to this amendment. Likewise, nutritional responses to mycorrhizal inoculation produced no discernible benefit in terms of seedling performance. An inoculation procedure that failed to induce substantially greater P. tinctorius colonization in inoculated than uninoculated seedlings, and that may have also impaired water relations, likely explains this result. Overall, these findings indicate that further research is needed before either the organic amendment or the mycorrhizal inoculation procedure used here can be used in forest restoration efforts on dry sites.     

Diversity and activity of methanotrophs in alkaline soil from a Chinese coal mine

Culture-independent molecular biological techniques, including 16S rRNA gene and functional gene clone libraries and microarray analyses using pmoA (encoding a key subunit of particulate methane monooxygenase), were applied to investigate the methanotroph community structure in alkaline soil from a Chinese coal mine. This environment contained a high diversity of methanotrophs, including the type II methanotrophs Methylosinus / Methylocystis , type I methanotrophs related to Methylobacter / Methylosoma and Methylococcus , and a number of as yet uncultivated methanotrophs. In order to identify the metabolically active methane-oxidizing bacteria from this alkaline environment, DNA stable isotope probing (DNA-SIP) experiments using ¹³CH₄ were carried out. This showed that both type I and type II methanotrophs were active, together with methanotrophs related to Methylocella , which had previously been found only in acidic environments. Methylotrophs, including Methylopila and Hyphomicrobium , were also detected in soil DNA and after DNA-SIP experiments. DNA sequence information on the most abundant, active methanotrophs in this alkaline soil will facilitate the design of oligonucleotide probes to monitor enrichment cultures when isolating key alkaliphilic methanotrophs from such environments.     

封闭环境下酸性矿坑水中微生物生态多样性的研究

铜绿山铜矿是世界开采时间最长的矿井之一,在开采过程中有许多矿井被废弃,许多废弃的矿井内产生了大量的对环境有害的酸性矿坑水.酸性矿坑水取自铜绿山铜矿某废弃矿井,利用限制性酶切片断多样性分析(RFLP分析)对酸性矿坑水中的微生物生态多样性进行了研究.研究表明,酸性矿坑水呈酸性,相对于其他极端与非极端生态环境,酸性矿坑水中的细菌与古菌的群落多样性较低.RFLP分析与系统发育分析表明,酸性矿坑水中细菌主要由A.fcrrooxidans(属于gamma-Proteobacteria)和L.ferrooxidans(属于Nitospira)成;古菌主要由Thermoplasma相关古菌组成.在这种封闭环境的酸性矿坑水中首次发现了类似于产甲烷古菌的克隆片断,其占古菌种群的四分之一左右.本研究将促进对酸性矿坑水中细菌及古菌群落组成及其对酸性矿坑水产生的作用的研究.     

采煤矿区的生态脆弱性——以内蒙古锡林郭勒草原胜利煤田为例

以锡林郭勒草原胜利煤田为典型研究区,构建了由生态敏感性、自然与社会压力及生态恢复力3方面16个因子组成的生态脆弱性评估指标体系,基于专家打分法和层次分析法建立了生态脆弱性模型,借助遥感及地理信息工具完成了对区域生态脆弱性指数的计算,分析了土地利用与生态脆弱性的关系,并通过空间自相关分析对计算结果进行了全局及局部聚类检验.结果表明： 研究区脆弱性总体属于中等偏高水平;胜利煤田4个露天矿的开采导致采区脆弱性显著增加,由于矿井疏干水和人为活动的影响,矿区周边300～2000 m范围都演变为生态高脆弱性区;随着矿区的进一步开发,整个煤田都将转变为中度和重度脆弱区,而煤炭资源开采是导致区域脆弱性提高的主要因素.全区及局部聚类结果显示,该区域脆弱性空间分布有很好的聚类特征.降低矿区人口密度、控制草地载畜水平、控制建设用地和耕地比率是解决矿区社会经济压力的最佳途径,增加投入、提高植被恢复系数是改变区域生态脆弱性的根本措施.
     

采煤塌陷区不同地貌类型植物群落多样性变化及其与土壤理化性质的关系

通过对神府-东胜矿区2种不同地貌类型下沉陷样地及对照样地地表植被进行调查,研究不同地貌类型、不同沉陷位置的群落多样性变化及其与土壤理化性质的关系。研究结果表明:(1)哈拉沟矿区(黄土丘陵地貌)植物种类较丰富,大柳塔矿区(风沙地貌)植被种类较单一;达乌里胡枝子作为风沙地貌沉陷区指示物种,其演替机制符合忍耐作用理论,紫翅猪毛菜作为黄土丘陵沟壑地貌沉陷区指示物种,其演替机制是促进理论和竞争共同作用的结果。(2)沉陷干扰12a后,2个研究区Shannon-Wiener指数分别增加了54.60%,73.85%,风沙地貌研究区土壤含水量和脲酶活性分别增加了8.64%和57.14%,黄土丘陵沟壑地貌研究区土壤有机质增加了47.73%。(3)采煤塌陷后,风沙地貌矿区坡中位置过氧化氢酶活性下降63.50%,土壤体积含水量降低58.70%,植物群落多样性显著降低;坡底位置土壤含水量、土壤养分、植物群落多样性均显著提高,土壤理化性质和植物群落多样性变化协同一致。(4)风沙地貌区植物群落多样性与土壤含水量存在显著正相关关系,黄土丘陵沟壑地貌区植物群落多样性与土壤有机质含量存在显著负相关关系。