ETHICAL DEBATE OVER ORGAN DONATION IN THE CONTEXT OF BRAIN DEATH

This study investigated what information about brain death was available from Google searches for five major religions. A substantial body of supporting research examining online behaviors shows that information seekers use Google as their preferred search engine and usually limit their search to entries on the first page. For each of the five religions in this study, Google listings reveal ethical controversy about organ donation in the context of brain death. These results suggest that family members who go online to find information about organ donation in the context of brain death would find information about ethical controversy in the first page of Google listings. Organ procurement agencies claim that all major world religions approve of organ donation and do not address the ethical controversy about organ donation in the context of brain death that is readily available online.     

Sensitivity of macroecological patterns of South American parrots to differences in data sources

A criticism of macroecological studies has been their extensive use of secondary data sources. In this note we evaluate how different data sources affect macroecological patterns for the parrots of South America. We mapped extents of parrot occurrence based on four sources of range maps. We compared basic statistics for geographical range size distribution (mean, variance and skew) and calculated correlations between geographical range size estimates and grid cell species richness estimates. Finally, results from multiple regression analyses of species richness against six environmental variables were also compared. We found that patterns were very robust to the data source, with only relatively slight quantitative differences. Our results reinforce the notion that patterns emerging from macroecological analyses are robust to variations in data sources and cannot be merely artefacts resulting from low data quality, notably poorly defined mapping and conflicting taxonomy.     

Food sources of Sand Martins Riparia riparia during their breeding season: insights from stable-isotope analysis

Capsule Stable-isotope analysis indicated that terrestrial dipteran insects were the main food at a site in northern Japan.     

Scale-up of a Type I secretion system in E. coli using a defined mineral medium

Secretion of heterologous proteins into the culture supernatant in laboratory strains of Escherichia coli is possible by utilizing a Type I secretion system (T1SS). One prominent example for a T1SS is based on the hemolysin A toxin. With this system, heterologous protein secretion has already been achieved. However, no cultivations in a defined mineral medium and in stirred tank bioreactors have been described in literature up to now, hampering the broad applicability of the system. In this study, a mineral medium was developed for cultivation under defined conditions. With this medium, the full potential and advantage of a secretion system in E. coli (low secretion of host proteins, no contamination with proteins from complex media compounds) can now be exploited. Additionally, quantification of the protein amount in the supernatant was demonstrated by application of the Bradford assay. In this work, host cell behavior was described in small scale by online monitoring of the oxygen transfer rate. Scalability was demonstrated by stirred tank fermentation yielding 540 mg/L HlyA1 in the supernatant. This work enhances the applicability of a protein secretion system in E. coli and paves the way for an industrial application.