全文获取类型
收费全文 | 815篇 |
免费 | 95篇 |
国内免费 | 50篇 |
出版年
2024年 | 3篇 |
2023年 | 25篇 |
2022年 | 22篇 |
2021年 | 46篇 |
2020年 | 40篇 |
2019年 | 60篇 |
2018年 | 41篇 |
2017年 | 36篇 |
2016年 | 30篇 |
2015年 | 31篇 |
2014年 | 42篇 |
2013年 | 59篇 |
2012年 | 26篇 |
2011年 | 27篇 |
2010年 | 23篇 |
2009年 | 39篇 |
2008年 | 36篇 |
2007年 | 47篇 |
2006年 | 27篇 |
2005年 | 36篇 |
2004年 | 32篇 |
2003年 | 25篇 |
2002年 | 17篇 |
2001年 | 19篇 |
2000年 | 9篇 |
1999年 | 6篇 |
1998年 | 11篇 |
1997年 | 12篇 |
1996年 | 6篇 |
1995年 | 5篇 |
1994年 | 6篇 |
1993年 | 7篇 |
1991年 | 11篇 |
1990年 | 6篇 |
1989年 | 6篇 |
1988年 | 7篇 |
1987年 | 7篇 |
1986年 | 5篇 |
1985年 | 14篇 |
1984年 | 13篇 |
1983年 | 6篇 |
1982年 | 12篇 |
1981年 | 8篇 |
1980年 | 1篇 |
1979年 | 3篇 |
1978年 | 4篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1974年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有960条查询结果,搜索用时 31 毫秒
61.
We tested the challenge hypothesis for the hormonal regulation of aggression in chicks of the black-headed gull, Larus ridibundus. Chicks of this species are highly aggressive toward conspecifics, but never to peers that hatched from the same clutch (modal clutch size is three). Therefore, in the first experiment small families were housed together in large groups (challenged condition) and compared to families kept isolated (nonchallenged condition). As expected, in the challenged condition during the initial stage of territory establishment basal levels of testosterone (T) were clearly higher than those in the nonchallenged condition. In the second experiment we tested the effect of a short social challenge on short-term T-fluctuations. The design was based on an earlier experiment, showing that after temporary T-treatment chicks become very sensitive to social challenges while having low basal T-levels. We now show that these social challenges induce brief elevations in plasma T-levels. These peaks are similar to those in previously untreated chicks but untreated chicks do not respond with aggression to a challenge. Therefore, we conclude that the initial exposure to elevated T-levels increases the sensitivity to brief changes in T induced by social challenges. In this way exposure to T, that may be detrimental for development, is minimized while birds remain able to defend territories. This is the first report showing that the challenge hypothesis as established for adult birds, is also applicable for aggressive behavior in young birds outside the sexual context. Furthermore we suggest that a phase of priming with T is necessary to obtain the high behavioral responsiveness to a challenge. 相似文献
62.
Yamazaki S Weinhold PS Graff RD Tsuzaki M Kawakami M Minchew JT Banes AJ 《Journal of cellular biochemistry》2003,90(4):812-818
Mechanical forces regulate the developmental path and phenotype of a variety of tissues and cultured cells. Vibratory loading as a mechanical stimulus occurs in connective tissues due to energy returned from ground reaction forces, as well as a mechanical input from use of motorized tools and vehicles. Structures in the spine may be particularly at risk when exposed to destructive vibratory stimuli. Cells from many tissues respond to mechanical stimuli, such as fluid flow, by increasing intracellular calcium concentration ([Ca(2+)](ic)) and releasing adenosine 5'-triphosphate (ATP), extracellularly, as a mediator to activate signaling pathways. Therefore, we examined whether ATP is released from rabbit (rAN) and human (hAN) intervertebral disc annulus cells in response to vibratory loading. ATP release from annulus cells by vibratory stimulation as well as in control cells was quantitated using a firefly luciferin-luciferase assay. Cultured hAN and rAN cells had a basal level of extracellular ATP ([ATP](ec)) in the range of 1-1.5 nM. Vibratory loading of hAN cells stimulated ATP release, reaching a net maximum [ATP] within 10 min of continuous vibration, and shortly thereafter, [ATP] declined and returned to below baseline level. [ATP] in the supernatant fluid of hAN cells was significantly reduced compared to the control level when the cells received vibration for longer than 15 min. In rAN cells, [ATP] was increased in response to vibratory loading, attaining a level significantly greater than that of the control after 30 min of continuous vibration. Results of the current study show that resting annulus cells secrete ATP and maintain a basal [ATP](ec). Annulus cells may use this nucleotide as a signaling messenger in an autocrine/paracrine fashion in response to vibratory loading. Rapid degradation of ATP to ADP may alternatively modulate cellular responses. It is hypothesized that exposure to repetitive, complex vibration regimens may activate signaling pathways that regulate matrix destruction in the disc. As in tendon cells, ATP may block subsequent responses to load and modulate the vibration response. Rabbit annulus cells were used as a readily obtainable source of cells in development of an animal model for testing effects of vibration on the disc. Human cells obtained from discarded surgical specimens were used to correlate responses of animal to human cells. 相似文献
63.
Interleukin-1 (IL-1) has been implicated in neuroimmune responses and has pleiotropic actions in the brain. Compelling evidence
has shown that IL-1 is a major mediator of inflammation and the progression of cell death in response to brain injury and
cerebral ischemia. Its expression is strongly increased in these pathological conditions, and central administration of exogenous
IL-1 significantly exacerbates ischemic brain damage. In contrast, inhibiting IL-1 actions (by intracerebroventricular [icv]
injection of IL-1ra, neutralizing antibody to IL-1 or caspase-1 inhibitor) significantly reduces ischemic brain damage. IL-1
acts by binding to the IL-1 type-1 receptor (IL-1RI), which is to date, the only known functional receptor for IL-1. However,
our recent investigations suggest that IL-1 can act independently of IL-1RI, raising the possibility that additional, as yet
undiscovered, receptor(s) for IL-1 exist in the brain. The recent characterization of putative, new IL-1 ligands and new IL-1
receptor-related molecules leads to the hypothesis that there might be alternative IL-1 signaling pathway(s) in the central
nervous system (CNS). 相似文献
64.
Brian J. Wilsey Terri B. Teaschner Pedram P. Daneshgar Forest I. Isbell H. Wayne Polley 《Ecology letters》2009,12(5):432-442
In many systems, native communities are being replaced by novel exotic-dominated ones. We experimentally compared species diversity decline between nine-species grassland communities under field conditions to test whether diversity maintenance mechanisms differed between communities containing all exotic or all native species using a pool of 40 species. Aboveground biomass was greater in exotic than native plots, and this difference was larger in mixtures than in monocultures. Species diversity declined more in exotic than native communities and declines were explained by different mechanisms. In exotic communities, overyielding species had high biomass in monoculture and diversity declined linearly as this selection effect increased. In native communities, however, overyielding species had low biomass in monoculture and there was no relationship between the selection effect and diversity decline. This suggests that, for this system, yielding behaviour is fundamentally different between presumably co-evolved natives and coevolutionarily naive exotic species, and that native-exotic status is important to consider. 相似文献
65.
Klára J. Petrželková Hideo Hasegawa Chris C. Appleton Michael A. Huffman Colleen E. Archer Liza R. Moscovice Mwanahamissi Issa Mapua Jatinder Singh Taranjit Kaur 《American journal of primatology》2010,72(4):307-316
The release of any species into a novel environment can evoke transmission of parasites that do not normally parasitize the host as well as potentially introducing new parasites into the environment. Species introductions potentially incur such risks, yet little is currently known about the parasite fauna of introduced primate species over the long term. We describe the results of long‐term monitoring of the intestinal parasite fauna of an unprovisioned, reproducing population of chimpanzees introduced 40 years earlier (1966–1969) onto Rubondo Island in Lake Victoria, Tanzania, a non‐native habitat for chimpanzees. Two parasitological surveys (March 1997–October 1998 and October 2002–December 2005) identified Entamoeba spp. including E. coli, Iodamoeba buetschlii, Troglodytella abrassarti, Chilomastix mesnili, Trichuris sp., Anatrichosoma sp., Strongyloides spp., Strongylida fam. gen. sp., Enterobius anthropopitheci, Subulura sp., Ascarididae gen. sp., and Protospirura muricola. The parasite fauna of the Rubondo chimpanzees is similar to wild chimpanzees living in their natural habitats, but Rubondo chimpanzees have a lower prevalence of strongylids (9%, 3.8%) and a higher prevalence of E. anthropopitheci (8.6%, 17.9%) than reported elsewhere. Species prevalence was similar between our two surveys, with the exception of Strongyloides spp. being higher in the first survey. None of these species are considered to pose a serious health risk to chimpanzees, but continued monitoring of the population and surveys of the parasitic fauna of the two coinhabitant primate species and other animals, natural reservoir hosts of some of the same parasites, is important to better understand the dynamics of host–parasite ecology and potential long‐term implications for chimpanzees introduced into a new habitat. Am. J. Primatol. 72:307–316, 2010. © 2009 Wiley‐Liss, Inc. 相似文献
66.
Laurence Meslet‐Cladiere Cedric Norais Regis Lavigne Didier Flament Rudolf Ladenstein Hannu Myllykallio 《The EMBO journal》2009,28(16):2479-2489
We show that Pyrococcus abyssi PAB2263 (dubbed NucS (nuc lease for s s DNA) is a novel archaeal endonuclease that interacts with the replication clamp PCNA. Structural determination of P. abyssi NucS revealed a two‐domain dumbbell‐like structure that in overall does not resemble any known protein structure. Biochemical and structural studies indicate that NucS orthologues use a non‐catalytic ssDNA‐binding domain to regulate the cleavage activity at another site, thus resulting into the specific cleavage at double‐stranded DNA (dsDNA)/ssDNA junctions on branched DNA substrates. Both 3′ and 5′ extremities of the ssDNA can be cleaved at the nuclease channel that is too narrow to accommodate duplex DNA. Altogether, our data suggest that NucS proteins constitute a new family of structure‐specific DNA endonucleases that are widely distributed in archaea and in bacteria, including Mycobacterium tuberculosis. 相似文献
67.
Piezoelectric poly(vinylidene fluoride) microstructure and poling state in active tissue engineering
下载免费PDF全文
![点击此处可从《Engineering in Life Science》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Clarisse Ribeiro Daniela M. Correia Sylvie Ribeiro Vítor Sencadas Gabriela Botelho Senentxu Lanceros‐Méndez 《Engineering in Life Science》2015,15(4):351-356
Tissue engineering strategies rely on suitable membranes and scaffolds, providing the necessary physicochemical stimuli to specific cells. This review summarizes the main results on piezoelectric polymers, in particular poly(vinylidene fluoride), for muscle and bone cell culture. Further, the relevance of polymer microstructure and surface charge on cell response is demonstrated. Together with the necessary biochemical cues, the proper design of piezoelectric polymers can open the way to novel and more reliable tissue engineering strategies for cells in which electromechanical stimuli are present in their environment. 相似文献
68.
69.
《Biological Rhythm Research》2007,38(6):399-414
Wistar rats maintained in cages with running wheels and submitted to a skeleton photoperiod or to a light - dark cycle were tested in the Morris water maze. Half of the animals were exposed to the task during their active phase while the other half was exposed during their inactive phase. The effect of the experience in the water maze, a strong arousing event, on the rhythm of wheel-running activity was evaluated. In the first experiment, a group of animals submitted to a skeleton photoperiod was trained every day in the reference memory version of the task. The novel experience in the water maze had a strong phase-dependent masking effect: it produced an intense post-training bout of activity in the animals tested during their inactive phase. Another experiment was run using single working memory sessions in the water maze and with animals submitted to a light - dark cycle. The circadian rhythm of locomotor activity was evaluated on undisturbed days and compared with testing days. The experience in the water maze produced a significant increase in variability of activity onset during both circadian phases. Taken together, the data suggest that there is a modulating effect of the arousing experience in the pool on the overt circadian rhythm of locomotor activity. 相似文献
70.
Bernd Spänhoff Christian Kock Alexander Meyer Elisabeth I. Meyer 《Physiological Entomology》2005,30(2):134-143
Abstract. The behaviour of larvae of Melampophylax mucoreus (Limnephilidae; Trichoptera) is studied to test their capacity for food detection over long distances by olfaction. In aquaria experiments with stagnant water, the larvae rapidly accumulate on a biofilm-covered stone (offered food patch) but not on control stones without biofilm-cover. Larvae in an artificial flow channel (flume), with uni-directional flow between an arrangement of stones covered with biofilm and stones without biofilm, can find food patches upstream from their location more rapidly than downstream. Stimuli transported towards the larvae by the water current might assist food location. However, no significant differences could be found in the movement of larvae with intact antennae compared with larvae without antennae. The results lead to the conclusion that the larvae of M. mucoreus find food patches mainly by random movement, which is assisted by their high mobility. Long-distance olfactory orientation towards food patches, mediated by antennae, can be precluded. Gustatory stimuli appear to play an important role in contact-chemoreception to identify biofilm patches on stone surfaces. Scanning electron microscopy studies of the larval head reveal that the antennae of M. mucoreus are small pegs (50 µm in length) with an apical plate (15 µm in diameter). The mouthparts of the larvae, especially the maxillary palps, show a variety of different sensilla types that are possibly used for contact-chemoreception. These sensilla also could be responsible for the orientation towards a food resource upstream of the larvae. 相似文献