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991.
Summary The presence of immunoreactive enkephalin, dynorphin, vasoactive intestinal polypeptide, cholecystokinin, substance P and neuropeptide Y in nerve fibers that project to the guinea-pig inferior mesenteric ganglion was analysed, after different denervation and ligation procedures. A quantitative analysis demonstrates that enkephalin- and substance P fibers reach the ganglion mainly via lumbar splanchnic and partly via intermesenteric nerves. Dynorphin-, vasoactive intestinal polypeptide- and cholecystokinin fibers reach the ganglion mainly via colonic and partly via hypogastric or intermesenteric nerves. Neuropeptide Y fibers enter via intermesenteric, lumbar splanchnic and hypogastric nerves and pass through the ganglion. Analysis of serial 0.5 m sections tends to confirm co-existence: of dynorphin, vasoactive intestinal polypeptide and cholecystokinin in fibers projecting from the colon; of dynorphin with substance P in the lumbar splanchnic nerves; and of neuropeptide Y with substance P in the hypogastric and colonic fibers. Synaptic contacts, predominantly axodendritic, onto the ganglion cells from enkephalin-, vasoactive intestinal polypeptide-, and substance P-containing terminals were revealed by electron microscopy. Enkephalin-immunoreactive axon varicosities are filled with small, clear vesicles with a few large, cored vesicles and form asymmetric synapses; dynorphin-, vasoactive intestinal polypeptide- and cholecystokinin-immunoreactive axon varicosities are rich in large, dense-cored vesicles and form symmetric synapses. 相似文献
992.
The social organization of the Bolivian squirrel monkey (Saimiri boliviensis boliviensis) is thought to be sexually segregated, with males and females forming separate social groups during the nonbreeding season. To investigate the influence of this social order on patterns of aggression, controlled single animal introductions within established groups and establishment of new groups were studied in a systematic manner. Behavioral observations were made using an all-occurrences technique to sample all agonistic interactions. In study I, 4 animals of each age-sex class (adult males and females, juvenile males and females) were introduced one at a time into 4 different social groups composed of 1 adult male and 8 to 10 adult females. Behavioral observations were made prior to and after the introductions. Newly introduced adult males received significantly more contact aggression than other age classes. Only the adult females increased aggression after the introduction of new animals. In study II, new social groups were formed and behavioral observations were made following formation. One group was formed from 7 pairs of familiar females and an unfamiliar adult male. This group had a high frequency of aggression during the first half hour, with contact aggression rising to peaks at 3 and 5 h. The second group consisted of 10 familiar females, 2 pairs of familiar females and an unfamiliar male. There was a significant peak in contact aggression 3 h into the observation. 相似文献
993.
袁岩 《分子细胞生物学报》1989,(3)
我们通过体外多聚腺苷酸化的大鼠正常肝7 s RNA,克隆了它的cDNA片段。其中一个克隆p 24的7 s cDNA插入顺序为180个碱基对,其顺序与大鼠Novikoff肝癌7 s RNA顺序比较,存在一个碱基的置换。用这个克隆提供的探针我们比较了大鼠肝细胞和肝癌细胞7 s RNA的基因拷贝数,基因组结构和在细胞内分布。结果表明:7 s RNA基因是多拷贝基因家族;7 s RNA在细胞癌变后含量下降;大约40%7 s RNA分布在细胞核内。我们还就7 s RNA具有调控基因表达功能的可能性进行了讨论。 相似文献
994.
Nicole Chaubet Gabriel Philipps Claude Gigot 《Molecular & general genetics : MGG》1989,219(3):404-412
Summary Five cloned histone H3 and H4 genes from maize have specific 5 non-transcribed regions. Blot hybridization of each 5 region to DNA from different maize inbred lines showed that the H3 and H4 multigenic families are organized into subfamilies. Each subfamily has a specific environment and contains a different (from 4–16) number of gene copies. H3 and H4 subfamilies with similar environments as those found in maize were shown to exist in the genomes of more or less related plants, including perennial teosinte, sorgho, sugar cane and Coïx. Such observations may contribute to establishing phylogenetic relationships at a molecular level between different plants and thus highlight some of the evolutionary mechanisms of the genomes of higher plants. 相似文献
995.
Cell migration and organization in three‐dimensional in vitro culture driven by stiffness gradient 下载免费PDF全文
996.
997.
Pablo R. Stevenson 《International journal of primatology》1998,19(2):299-311
I studied proximal spacing within a group of woolly monkeys (Lagothrix lagotricha) during 7 months at Parque Nacional Tinigua, Colombia. I collected a total of 1188 instantaneous samples on focal individuals, recording the number and age/sex class of individuals that were in contact with, <2 m from, <5 m from the focal animal. The results indicate that proximate spacing reflects social affinities and is related to mother–infant relationship and social grooming. Subadult females and adult males are the sex/age classes with the lowest number of individuals in proximity. There are low proximity between adult females and between adult males and high frequencies of nearness between mother and offspring. Associations between males and females were usually low, but in some cases males showed preferences for a given female. There was a relatively gradual increase in spacing between mothers and their offspring as they became older. Old juvenile males were associated chiefly with other males—mostly subadults—whereas juvenile females maintained some proximity only to their mothers. There are also differences in spacing behavior according to different activity types. 相似文献
998.
One of the most important omissions in recent evolutionary theory concerns how eukaryotes could emerge and evolve. According
to the currently accepted views, the first eukaryotic cell possessed a nucleus, an endomembrane system, and a cytoskeleton
but had an inefficient prokaryotic-like metabolism. In contrast, one of the most ancient eukaryotes, the metamonada Giardia lamblia, was found to have formerly possessed mitochondria. In sharp contrast with the traditional views, this paper suggests, based
on the energetic aspect of genome organization, that the emergence of eukaryotes was promoted by the establishment of an efficient
energy-converting organelle, such as the mitochondrion. Mitochondria were acquired by the endosymbiosis of ancient α-purple
photosynthetic Gram-negative eubacteria that reorganized the prokaryotic metabolism of the archaebacterial-like ancestral
host cells. The presence of an ATP pool in the cytoplasm provided by this cell organelle allowed a major increase in genome
size. This evolutionary change, the remarkable increase both in genome size and complexity, explains the origin of the eukaryotic
cell itself.
The loss of cell wall and the appearance of multicellularity can also be explained by the acquisition of mitochondria. All
bacteria use chemiosmotic mechanisms to harness energy; therefore the periplasm bounded by the cell wall is an essential part
of prokaryotic cells. Following the establishment of mitochondria, the original plasma membrane-bound metabolism of prokaryotes,
as well as the funcion of the periplasm providing a compartment for the formation of different ion gradients, has been transferred
into the inner mitochondrial membrane and intermembrane space. After the loss of the essential function of periplasm, the
bacterial cell wall could also be lost, which enabled the naked cells to establish direct connections among themselves. The
relatively late emergence of mitochondria may be the reason why multicellularity evolved so slowly.
Received: 29 May 1997 / Accepted: 9 October 1997 相似文献
999.
H. I. Stewart N. J. O'Neil D. L. Janke N. W. Franz H. M. Chamberlin A. M. Howell E. J. Gilchrist T. T. Ha L. M. Kuervers G. P. Vatcher J. L. Danielson D. L. Baillie 《Molecular & general genetics : MGG》1998,260(2-3):280-288
The central gene cluster of chromosome III was one of the first regions to be sequenced by the Caenorhabditis elegans genome project. We have performed an essential gene analysis on the left part of this cluster, in the region around dpy-17III balanced by the duplication sDp3. We isolated 151 essential gene mutations and characterized them with regard to their arrest stages. To facilitate positioning
of these mutations, we generated six new deficiencies that, together with preexisting chromosomal rearrangements, subdivide
the region into 14 zones. The 151 mutations were mapped into these zones. They define 112 genes, of which 110 were previously
unidentified. Thirteen of the zones have been anchored to the physical sequence by polymerase chain reaction deficiency mapping.
Of the 112 essential genes mapped, 105 are within these 13 zones. They span 4.2 Mb of nucleotide sequence. From the nucleotide
sequence data, 920 genes are predicted. From a Poisson distribution of our mutations, we predict that 234 of the genes will
be essential genes. Thus, the 105 genes constitute 45% of the estimated number of essential genes in the physically defined
zones and between 2 and 5% of all essential genes in C. elegans.
Received: 23 April 1998 / Accepted: 18 August 1998 相似文献
1000.
Scanning electron microscopical investigations of fractures and corrosion casts of spirales in adult ticks of Hyalomma truncatum revealed a three-part structure consisting of the spiracular plate forming the outer part followed by the subostial space, which leads into the atrial chamber from which the main tracheal trunks arise. The spiracular plate sonsists of a thin surface plate perforated by aeropyles, an underlying interpedicellar space formed by pedicels and an inner thick base plate. The surface plate is subdivided into a porous and a non-porous area. The macula is surrounded by the porous area and cleft by the ostium, which is bounded by a lip. The lip rests on a stalk which passes through the subostial space and forms the lateral wall of the atrial chamber. The interpedicellar space is chambered comprising four types of chambers. Large pyriform chambers (type 1) open to the atmosphere via a large aeropyle and are connected at their base with a duct traversing the base plate. They correspond numerically and in their position with the large aeropyles and the ducts of the base plate. Each chamber is surrounded by four to six medium-sized tubular chambers (type 2) which are closed at both ends. Small tubular chambers (type 3) open to the atmosphere via a small aeropyle, are closed at their base and correspond in number and position to the small aeropyles. Elongated chambers (type 4) are arranged in two to three rows around the subostial space and are closed at both ends. The front row communicates with the subostial space via large gaps. All chambers interconnect with each other by slit-like fenestrations. Below the macula and surrounding the stalk is the subostial space. Over the medial half, the subostial space opens into the atrial chamber. The lateral wall of the atrial chamber is thick, whereas the opposite wall is thin, folded and can be everted and inverted. Inverted, the medial wall closes up the opening to the subostial space and the main tracheal trunks. The base of the atrial chamber sonsists of the openings of the main tracheal trunks only. It is concluded that the aeropyles constitute the functional openings of a spiracle, the interpedicellar space and the subostial space act as diffusion barrier and the atrial chamber is exclusively responsible for the motory process of in- and expiration and is the only closing device of the spiracle. 相似文献