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71.
Summary To optimize culture conditions and gain a more reliable culturing system for studies of metabolic properties of neuronal cells,
a simplified perfusion chamber was developed. It consists of two parts: a perfusion block and a standard plastic culture dish.
To confirm the suitability of this chamber for continuous culturing of anchorage-dependent cells, the growth and morphology
of the four neuronal cell lines glioma C6 and glioma 138MG, neuroblastoma C1300, clones N1E115 and N18 were followed for 4
d using both traditional and perfusion techniques. A marked increase in growth and a decrease in the degree of morphological
differentiation were obtained with the latter technique compared to the former.
This work was supported by grants from the National Swedish Board for Technical Development (Grant 81-5009), the Swedish Work
Environmental Foundation (Grant 76-53), and Ollie and Elof Ericssons Foundation for Scientific Research. 相似文献
72.
We report the production of a monoclonal antibody (MAb 526) that recognizes a novel, developmentally regulated nuclear protein expressed in neurons throughout the rat nervous system. Analysis of whole brain and cell nuclear extracts by SDS-PAGE and immunoblotting determined that MAb 526 recognizes a single nuclear protein (np) of apparent molecular weight 42 kD, designated np526, as well as a slightly larger (ca. 44 kD) cytoplasmic protein. Light microscopic immunocytochemistry showed np526 to be present in neurons of all types throughout the central and peripheral nervous systems. Nuclei of both fibrous and protoplasmic astrocytes were also immunoreactive, but oligodendrocyte nuclei were negative. Positive, but highly variable immunocytochemical staining of nonneural cell nuclei in a variety of other tissues was also observed. Electron microscopic (EM) immunocytochemistry using pre-embedding peroxidase methods revealed that np526 is associated with euchromatin or with the edges of condensed chromatin bundles in neurons, indicating that it is likely to be a chromosomal protein. Most interestingly, the expression of np526 was found to be developmentally regulated in brain. Immunocytochemical analysis of the developing cerebral cortex from embryonic day (E) 16 to postnatal day (P) 4 and cerebellum from P4 to P18 revealed that np526 first appears in central neurons following the cessation of mitosis and that the intensity of nuclear staining increases during subsequent neuronal maturation. To our knowledge, np526 is the first presumptive chromosomal protein whose expression has been precisely correlated with the early postmitotic differentiation of mammalian neurons. 相似文献
73.
Bernhard H. J. Juurlink Sergey Fedoroff 《In vitro cellular & developmental biology. Plant》1979,15(2):86-94
Summary Whole mouse embryos were grown in vitro from Theiler stage 12 (1 to 7 somites) to Theiler stages 15 and 16 (25 to 35 somites).
This procedure gives experimental access to precisely staged embryos during the early period of neurogenesis. To follow the
further development of neurons in vitro, fragments of spinal primordia were set up from these cultured embryos. In such cultures,
the proliferation of precursor cells, the formation of postmitotic cells and, finally, the cytodifferentiation of neurons
were observed.
A preliminary account of this work was given at the Tissue Culture Association Meeting in 1977, and the Canadian Federation
of Biological Societies Meeting in 1977 (1,2).
This work was supported by Grant MT 4235 from the Medical Research Council of Canada. 相似文献
74.
ZAKβ antagonizes and ameliorates the cardiac hypertrophic and apoptotic effects induced by ZAKα
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Chien‐Yao Fu Wei‐Wen Kuo Tsung‐Jung Ho Su‐Ying Wen Ling‐Chun Lin Yan‐Shen Tseng Hui‐Chuan Hung Vijaya Padma Viswanadha Chih‐Yang Huang 《Cell biochemistry and function》2016,34(8):606-612
ZAK (sterile alpha motif and leucine zipper containing kinase AZK), a serine/threonine kinase with multiple biochemical functions, has been associated with various cell processes, including cell proliferation, cell differentiation, and cardiac hypertrophy. In our previous reports, we found that the activation of ZAKα signaling was critical for cardiac hypertrophy. In this study, we show that the expression of ZAKα activated apoptosis through both a FAS‐dependent pathway and a mitochondria‐dependent pathway by subsequently inducing caspase‐3. ZAKβ, an isoform of ZAKα, is dramatically expressed during cardiac hypertrophy and apoptosis. The interaction between ZAKα and ZAKβ was demonstrated here using immunoprecipitation. The results show that ZAKβ has the ability to diminish the expression level of ZAKα. These findings reveal an inherent regulatory role of ZAKβ to antagonize ZAKα and to subsequently downregulate the cardiac hypertrophy and apoptosis induced by ZAKα. 相似文献
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78.
A. E. King 《Neurophysiology》2006,38(5-6):410-420
The use of amphibian and mammalian in vitro spinal cord preparations, e.g., hemisected cord and transverse slices, has gained in popularity over the years due to the
flexibility and ease of use of such preparations compared to classical in vivo approaches. When combined with modern experimental methodologies, such as patch clamping of visualized single cells or post-experimental
neuroanatomy, this approach provides a powerful addition to the armamentarium available to study nociceptive processing in
the dorsal horn. Some of these novel experimental approaches and the insight into nociception that they have provided are
described below.
Neirofiziologiya/Neurophysiology, Vol. 38, Nos. 5/6, pp. 481–491, September–December, 2006. 相似文献
79.
The physicochemical properties and chemical constituents of the blue protein from rice bran were investigated. The blue protein was a copper-containing glycoprotein, the molecular weight of which was found to be 18,300 Daltons by the sedimentation equilibrium method assuming the partial specific volume 0.72 cm3 g?1. The hexose and pentose contents were 5.49 and 4.01 g per 100 g protein respectively. The copper content was 0.38% which corresponded to 1.09 atoms per one molecule of the protein. The electron spin resonance spectrum showed that the copper was in a cupric state. The standard oxidation-reduction potential of the copper was found to be +275 mV at 20°C and at pH 7.39. The visible and near infrared absorption maxima were found at 450, 600 and 890 mμ, and the 450 mμ band was optically active in the optical rotatory dispersion exhibiting a large Cotton effect. 相似文献
80.
《Current biology : CB》2020,30(11):2166-2174.e3
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