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81.
Microbial growth on carbon monoxide 总被引:14,自引:0,他引:14
The utilization of carbon monoxide as energy and/or carbon source by different physiological groups of bacteria is described and compared. Utilitarian CO oxidation which is coupled to the generation of energy for growth is achieved by aerobic and anaerobic eu- and archaebacteria. They belong to the physiological groups of aerobic carboxidotrophic, facultatively anaerobic phototrophic, and anaerobic acetogenic, methanogenic or sulfate-reducing bacteria. The key enzyme in CO oxidation is CO dehydrogenase which is a molybdo iron-sulfur flavoprotein in aerobic CO-oxidizing bacteria and a nickel-containing iron-sulfur protein in anaerobic ones. In carboxidotrophic and phototrophic bacteria, the CO-born CO2 is fixed by ribulose bisphosphate carboxylase in the reductive pentose phosphate cycle. In acetogenic, methanogenic, and probably in sulfate-reducing bacteria, CODH/acetyl-CoA synthase directly incorporates CO into acetyl-CoA.In plasmid-harbouring carboxidotrophic bacteria, CO dehydrogenase as well as enzymes involved in CO2 fixation or hydrogen utilization are plasmid-encoded. Structural genes encoding CO dehydrogenase were cloned from carboxidotrophic, acetogenic and methanogenic bacteria. Although they are clustered in each case, they are genetically distinct.Soil is a most important biological sink for CO in nature. While the physiological microbial groups capable of CO oxidation are well known, the type and nature of the microorganisms actually representing this sink are still enigmatic. We also tried to summarize the little information available on the nutritional and physicochemical requirements determining the sink strength. Because CO is highly toxic to respiring organisms even in low concentrations, the function of microbial activities in the global CO cycle is critical. 相似文献
82.
ACladosporium species produced large amounts of cellulase enzyme components when grown in shake-culture with medium containing carboxymethylcellulose. There was significantly less activity when Avicel, filter paper or cotton were used as substrates. KNO3 was better than NH4Cl or urea for the production of cellulase. Tween 80 at 0.1% (w/v) increased the production of cellulase by 1.5 to 4.5-fold. All the cellulase components were optimally active in the assay at pH 5.0 and 60°C. 相似文献
83.
R. R. Colwell 《Biodiversity and Conservation》1992,1(4):342-345
Concluding remarks from the joint IUBS/IUMS workshop on Biodiversity amongst microorganisms and its relevance held in Amsterdam on 7–8 September 1991. An international microbial ecology programme can be justified in its own right now that appropriate investigative tools have been developed. Microorganisms influence global change, and indicate global health and environmental quality. At the same time, an inventory of the world's microbial species and their properties is required, together with associated culture collections and genomes. Sampling methods need to be standardized, both for species and functions. Extreme environments are a particularly rich source of microbial genomes, and endangered habitats should be sampled as a matter of priority. Cataloguing and conserving the world's microbial biodiversity is justifiable and scientifically important. 相似文献
84.
Antarctic terrestrial ecosystems are briefly described, with emphasis on Signy Island in the maritime antarctic region, and the McMurdo oasis, southern Victoria Land, and Vestfold Hills in the continental antarctic region.As the largest and best known coastal ice-free oasis, the Vestfold Hills contain excellent examples of terrestrial sublithic, epilithic, chasmoendolithic, epiphytic and terricolous algal communities, as well as epilithic, endolithic, and epiphytic lichen communities, and moss communities. Many of the numerous lakes support dense communities of aquatic algae. 相似文献
85.
Variation in the carbon content of two Asplanchna species 总被引:3,自引:3,他引:0
The rotifers of the genus Asplanchna were sampled four times during the summer from eight lakes of different types. The mean individual carbon content in the population varied between 0.15–0.66 µg C ind.-1 (n = 21) for A. priodonta and 1.0–1.6 µg C ind.-1 (n = 3) for A. herricki. The carbon content and the size of A. priodonta varied considerably between the populations of both different lakes and dates.The carbon level of both Asplanchna species (sample mean 0.2–1% of wet weight) was considerably lower than is generally found for rotifers. Much of the variation of carbon level could be explained by an inverse relationship with wet weight. The high variation in the carbon content of individuals suggests that Asplanch population may adapt their mean body size to fit prevailing environmental conditions. 相似文献
86.
Factors influencing nitrate depletion in a rural stream 总被引:3,自引:3,他引:0
Alan R. Hill 《Hydrobiologia》1988,160(2):111-122
A mass balance procedure was used to analyze rates of nitrate depletion in three adjacent reaches of West Duffin Creek, Ontario, Canada. Daily nitrate losses in individual reaches were highly variable (0.5–24 kg N) during low and moderate stream flows in May–October, 1982–1985. Nitrate removal efficiency (nitrate loss as a % of nitrate input) showed a rapid exponential decline with increased nitrate inputs to each reach. Nitrate losses and nitrate removal efficiency also had a significant negative correlation with stream discharge. The association of large nitrate loads with high stream discharge reduced the nitrate removal capacity of the stream because of shorter residence times and a higher ratio of water volume to stream bed area. Water temperature exhibited a significant positive correlation with nitrate loss which may reflect increased denitrification at higher temperatures.Variations in nitrate losses and nitrate removal efficiency between the three reaches were highly influenced by differences in water residence time. Standarized nitrate losses with respect to water residence time revealed a longitudinal decline in nitrate depletion between the reaches which was associated with a downstream decrease in stream nitrate concentration and in the organic carbon content of fine textured sediments from pool habitats. 相似文献
87.
Sture Hansson 《Hydrobiologia》1988,160(2):107-110
A new technique for suspending vertical gill nets is described. The system is designed to minimize the effects of currents, which otherwise could lift the lead line off the bottom or pull the floating line down.For more than two years, 30 m deep gill nets have been used in a Baltic Sea coastal region and have been found to work well in current, wave and wind conditions characteristic of this area. Setting a net takes about 5 minutes and retrieving it 10 minutes, even under relatively windy conditions (10–15 ms–1). No special equipment is required to set or to haul a net, and we have used a variety of boats, from a small rowing boat to a 16 m long research vessel. The total material cost per net is about US $100. 相似文献
88.
Water hyacinth productivity and detritus accumulation 总被引:2,自引:1,他引:1
Water hyacinth [Eichhornia crassipes (Mart) Solms] productivity and detritus accumulation were evaluated in eutrophic lake water with and without added nutrients
(fertilized and control reservoirs, respectively). Seasonal changes in plant productivity and detritus accumulation were determined
at monthly intervals for one year.
Significant differences were observed in plant productivity between seasons and nutrient additions. Seasonal plant productivity
ranged from 1.9 to 23.1 mg (dry wt) ha−1 for the fertilized reservoir and −0.2 to 10.2 mg ha−1 for the control reservoir. Detritus accumulation was not significantly different between seasons or nutrient additions. Seasonal
N assimilation by plants ranged from 34 to 242 kg N ha−1 for plants in the fertilized reservoir and < 0 to 104 kg N ha−1 for plants in the control reservoir. Annual net N recovered in detritus represented 21 and 28% of the total N removed by
plants in the fertilized and control reservoirs, respectively. Net N loading to the reservoirs from detritus was 92 to 148
kg N ha−1 yr−1. 相似文献
89.
A method is described for the measurement of the carbon and nitrogen content of particulate material in natural waters. Particulate material is separated by filtration through GF/C filters. The dried filter is encapsulated in silver foil using a purpose made press. Analysis is carried out using high temperature combustion with thermal conductivity detection of emission gasses. Analytical performance characteristics obtained with both standards and natural materials are given. 相似文献
90.
Respiration was measured in dauer stages of the insect-parasitic nematode Steinernema feltiae (= Neoaplectana carpocapsae) at 7, 17, and 27 C. Respiration, Q₁₀, and nematode viability were temperature dependent. Mean O₂ consumption for 5 × 10⁵ nematodes the first 24 hours was 0.27 ml at 7 C, 0.83 ml at 17 C, and 2.68 ml at 27 C. The Q₁₀ was 3.10 for 7-17 C and 3.24 for 17-27 C. Some nematodes died during 2, 14, and 21 days at 27, 17, and 7 C, respectively. The respiratory quotient was below 1 at all temperatures tested. A standard asymptotic model is expressed as oxygen consumed = 2.77 * {1 - exponent[-time * exponent(-B + C * temperature)]}; where 2.77 is the maximum response at 27 C. This model estimates nematode O₂ consumption and viability at storage temperatures between 7 and 27 C. The nematodes died when the O₂ concentration reached 0.5 ml/5 × 10⁵ nematodes. This model may be used to predict O₂ requirements of S. feltiae infective juveniles when stored as a waterless concentrate. 相似文献