三阴性乳腺癌中MMP9和P53的表达与其预后的关系研究

目的:研究三阴性乳腺癌(triple negative breast cancer,TNBC)中基质金属蛋白酶9(matrix metal proteinase 9,MMP9)和P53的表达及其与三阴性乳腺癌预后的关系。方法:收集2002年3月至2011年7月上海交通大学医学院附属新华医院普外科诊治且临床资料完整的TNBC标本147例,通过免疫组化检测其MMP9、P53的表达。结合临床随访数据,分析MMP9、P53的表达与TNBC预后的关系。结果:TNBC占同期本院收治的乳腺癌13.3%(147/1103)。MMP9、P53在TNBC中的阳性表达率分别为47.6%和66.0%。MMP9与TNBC患者的淋巴结转移数显著相关(P=0.003)。MMP9表达阳性的TNBC与MMP9表达阴性TNBC患者60个月的无病生存(disease free survival,DFS)率分别为62%和83%,差异具有统计学意义(P=0.001);总体生存率(overall survival,OS)率分别为66%和73%,差异具有统计学意义(P=0.022)。P53表达阳性的TNBC和P53表达阴性的TNBC患者60个月的DFS分别为65%和87%(P=0.010);OS分别为60%和85%(P=0.005)。MMP9和P53的HR分别为3.353(95%CI:1.614-6.966,P=0.001)和2.979(95%CI:1.239-7.165,P=0.015)。结论:MMP9和P53在TNBC中的阳性表达与患者的预后不良有关,且MMP9为影响TNBC患者预后的独立危险因素。     

Estimating density of a forest-dwelling bat: a predictive model for Rafinesque’s big-eared bat

Data on species distribution and abundance are the foundation of population ecology. However, due to difficulties in surveying bats, abundance estimates for tree-roosting microchiropterans are non-existent. Therefore, our objective was to develop methods for estimating colony abundance and density, taking as our model Rafinesque’s big-eared bat Corynorhinus rafinesquii, a species of conservation concern found in cypress-gum swamps of the southeastern United States. We searched 123 transects at eight study sites in the Coastal Plain of Georgia, USA to locate and characterize diurnal summer roosts of C. rafinesquii. We modeled the relationship between the number of bat colonies and landscape-scale habitat variables with zero-inflated negative binomial regression and used Akaike’s information criterion to select the most parsimonious models. We generated a predictive density map to identify areas of high colony density and to estimate overall abundance. Colony density was predicted by the duration of wetland flooding, wetland width, and study site. Application of the regression model to a GIS indicated there were 3,734 colonies containing 6,910 adult bats on the eight study sites. Predicted density ranged from 0.07 colonies/ha and 0.07 adult bats/ha in saturated wetlands to 0.47 colonies/ha and 1.18 adult bats/ha in semi-permanently flooded wetlands. This study is the first to estimate density and abundance of forest-dwelling microchiropterans over a large area. Such data can serve as a baseline for future work on population trends in C. rafinesquii. In addition, our approach could be replicated for other bat species with moderately cryptic roosts.     

The utility of inflammation and platelet biomarkers in patients with acute coronary syndromes

Introduction

Thrombotic and inflammatory mechanisms are involved in the pathophysiology of acute coronary syndrome (ACS). The aim of the study was the evaluation of inflammation (white blood cells count/WBC, C-reactive protein/CRP, interleukin-6/IL-6) and platelet (platelet count/PLT, mean platelet volume/MPV, large platelet/LPLT, beta-thromboglobulin/β-TG) biomarkers in the groups of ACS patients depending on the severity of signs and symptoms and compared to controls without coronary artery disease.

Automated Recording of Vertical Negative Phototactic Behaviour in Daphnia magna Straus (Crustacea)

Diurnal vertical migration is a well-known phenomenon in the circadian activity rhythms of zooplankton. Our goal was to test whether negative phototaxis in Daphnia magna clone BEAK (provoked by artificially induced light stress, alternating light and dark phases in 2 h intervals), and its interference with the endogenous rhythm of diurnal vertical migration, can be automatically registered with a biomonitor. For the first time the vertical swimming behaviour of D. magna was recorded quantitatively based on non-optical data recording in a fully automated biotest system, the Multispecies Freshwater Biomonitor in a new experimental setup consisting of a column of three recording units (3-level chambers). Circadian vertical migration was clearly recorded in the 3-level chambers and the rhythm was more clear with 5 than with 1 organism per chamber. The organisms clearly responded to induced light stress with negative phototaxis, however best in larger chambers. The artificially induced rhythm was influenced by the endogenous rhythm. This approach may facilitate long-term observations of vertical swimming activity of zooplankton in the future.     

间断下体负压暴露方式对下体负压耐力的影响

目的：探讨不同方式反复下体负压锻炼对下体负压耐力的影响,以期筛选最佳的负压锻炼方式。方法：27名男性健康受试者随机分成3组,分别进行－5.33kPa8min(A组）、6.67kPa4min（B组）、6.67kPa8min（C组）的下体负压锻炼后累积应激指数（CSI）、总耐受时间（DNP）较锻炼前显著提高,A、B组上述指标无显著变化,下体负压暴露时的心率较平静状态显著升高,收缩压显著降低,舒张压无显著变化。结论：经过－6.67kPa/d8min连续8d的间断下体负压可以显著提高下体负压耐力。     

Antimicrobial effect of different herbal plant extracts against different microbial population

This study evaluates the antimicrobial effects of ethanolic extract of five herbal plants; Guava (Psidium guajava), Sage (Salvia officinalis), Rhamnus (Ziziphusspina Christi), Mulberry (Morusalba L.), and Olive (Oleaeuropaea L) leaves against several microbial population representing Gram positive, Gram negative and Mollicutes; S. aureus, E. coli, Pasteurella multocida, B. cereus, Salmonella Enteritidis and M. gallisepticum using standard agar disc diffusion technique and minimal inhibitory concentration (MIC). Different extracts reveal variable results against the microorganism under study. All extracts have no antibacterial potency for Mycoplasma gallisepticum except Psidium guajava. The results of minimal inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) of the extracts against the six bacteria ranged from 625 to 5000 μg/ml. The used herbal extract could inhibit the selected microorganism under study with variable minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).     

脉冲电场作用对植物释放负离子与气孔特征的关系

植物在自然状态下释放负离子的能力很弱,施加脉冲电场可激发其释放能力。在密闭的玻璃箱中,研究紫背竹芋(Stromanthe sanguinea)、绒叶肖竹芋(Calathea zebrina)和朱顶红(Hippeastrum rutilum)在常态、脉冲电场和光照刺激下释放负离子的浓度,并观察叶片气孔特征,结果表明:(1)不同参数脉冲电场对植物释放负离子的能力影响不同,每种植物均具有高效释放负离子的最佳脉冲电场,紫背竹芋为A3B3C3(A3,U=1.5×104 V;B3,T=1.5 s;C3,?=65 ms);绒叶肖竹芋为A3B4C1(A3,U=1.5×104 V;B4,T=2.0 s;C1,?=5 ms);朱顶红为A4B4C1(A4,U=2.0×104 V;B4,T=2.0 s;C1,?=5 ms)。(2)植物体上所储存的电压越大,其释放负离子的能力越强。(3)脉冲电场作用时,植物释放负离子的能力与光照度呈正相关;无电场刺激时两者差异不显著(P0.05)。(4)植物释放负离子的能力与叶片气孔特征关系密切,脉冲电场作用下叶片气孔的开合度和气孔密度越大,其释放能力越强。     

外源突变基因AGM3过表达对烟草开花的抑制和花器官发育的影响

为了解AGM3基因在异源植物中对开花及花器官发育的影响,采用农杆菌介导法将dominant negative mutation(DNM)结构基因35S-AGM3-E9导入烟草(Nicotiana tabacum),经PCR和Southern检测获得了一批阳性转化植株.荧光定量分析结果显示,AGM3在各个转基因株系中均有...     

落叶松人工林土壤热传导季节变化及其与环境因子的关系

以2003—2005年落叶松人工林土壤热状况的实际监测数据为基础,研究了其土壤热传导特性及其与环境因子的关系.结果表明：落叶松人工林不同年份、不同地点土壤热通量的季节变化十分明显,4—8月为正值,9月至次年3月的绝大部分时间为负值,其年收支基本处于平衡状态.土壤热通量和土壤热导率（k）受净辐射影响显著,并存在时间延迟效应,冬季为4～5 h,夏季为2～3 h.基于实测的土壤热通量和土壤温度差计算得到的k值早春季节显著高于其他季节（P<0.05）,而其他月份不存在显著差异(P>0.05).使用我国大多数气象观测站积累的土壤温差数据和 k值进行土壤热通量季节变化估计时,早春季节（3—5月）可能会产生较大的误差.     

Comparison of cell lines for stable production of fucose-negative antibodies with enhanced ADCC

Several methods have been described to enhance antibody-dependent cellular cytotoxicity (ADCC) using different host cells that produce antibody with reduced levels of fucose on their carbohydrates. We compared the suitability of these methods for the serum-free fed-batch production of antibody for clinical trials and commercial uses. Recombinant anti-human CD20 chimeric IgG1-producing clones were established from host-cells that have been shown to produce more than 90% fucose-negative antibody. The cell lines were a FUT8 (alpha-1,6-fucosyltransferase) knockout Chinese hamster ovary (CHO) cell line, Ms704, and two Lens culinaris agglutinin (LCA)-resistant cell lines, one derived from a variant CHO line, Lec13 and the other from a rat hybridoma cell line, YB2/0. The amount of fucose-negative antibody produced by Lec13 and YB2/0 significantly decreased with the culture. The increase in fucosylation was due to remaining synthesis of GDP-fucose via de novo pathway for the CHO line and the elevation of FUT8 expression by the YB2/0 cells. In contrast, Ms704 cells stably produced fucose-negative antibody with a consistent carbohydrate structure until the end of the culture. The productivity of the Ms704 cells reached 1.76 g/L with a specific production rate (SPR) of 29 pg/cell/day for 17 days in serum-free fed-batch culture using a 1 L spinner bioreactor. Our results demonstrate that FUT8 knockout has the essential characteristics of host cells for robust manufacture of fucose-negative therapeutic antibodies with enhanced ADCC.