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191.
192.
Russell AF Carlson AA McIlrath GM Jordan NR Clutton-Brock T 《Evolution; international journal of organic evolution》2004,58(7):1600-1607
In species of cooperative insects that live in large groups, selection for increased fecundity has led to the evolution of an increased body size among female reproductives, but whether this is also true of cooperative vertebrates is unknown. Among vertebrates, morphological modification of female breeders has only been documented in a single species; in naked mole rats (Heterocephalus glaber), acquisition of alpha status is associated with a significant increase in body size through an elongation of the lumbar vertebrae. Here we provide evidence of morphological modification among breeding females of a cooperative carnivore, the meerkat (Suricata suricatta), and demonstrate that this modification is likely to be adaptive. The same female meerkats were significantly larger when they were dominant than when they were subordinate. This increased body size was not explained by differences in age, foraging efficiency, or investment in offspring care, but may have arisen, in part, through increased levels of hormone that govern bone growth. Increases in body size are likely to result in fitness benefits, for large females delivered larger litters and had heavier offspring, both of which are known to correlate positively with measures of breeding success in meerkats. Our results suggest that the acquisition of alpha status in female meerkats is associated with an adaptive increase in body size and hence that morphological modification of female vertebrates may be more widespread than has been previously supposed. 相似文献
193.
Phytotoxicity of aluminum (Al) is the major limiting factor for the crops grown in acid soils rapidly inhibiting root elongation.
In this study, changes in root growth, total activity and isozyme patterns of antioxidant enzymes such as peroxidase, ascorbate
peroxidase, catalase and glutathione reductase by Al stress were investigated in the roots of naked barley (Hordeum vulgare L. cv. Kwangwhalssalbori). As Al concentration increased up to 500 M, the rooting rate and root elongation substantially
decreased. Growth results suggested that this cultivar is an Al-sensitive species. Total activities of antioxidant enzymes
generally increased at lower Al concentrations and then gradually decreased at higher Al concentrations. They also increased
when the exposure time to Al was extended up to 48 hr. Changes in the isozyme patterns of antioxidant enzymes were investigated
byin situ enzyme activity staining on a non-denaturing PAGE gel. They generally coincided with the changes in the total activity in
parallel. Changes in the total activity of antioxidant enzymes also coincided with the changes of the root growth. Since growth
reduction in the roots by Al stress could be related with the changes in the activities of antioxidant enzymes, these results
suggested that Al might cause the oxidative stress in the roots of this cultivar of naked barley. 相似文献
194.
195.
Glasspool-Malone J Steenland PR McDonald RJ Sanchez RA Watts TL Zabner J Malone RW 《The journal of gene medicine》2002,4(3):323-322
Background
Nuclease activity present within respiratory tissues contributes to the rapid clearance of injected DNA and therefore may reduce the transfection activity of directly injected transgenes. Most gene transfer technologies transduce or transfect murine tissues more efficiently than corresponding primate tissues. Therefore, it is prudent to assess the utility of novel gene transfer strategies in both rodent and primate models before proceeding with further development.Methods
This study analyzed the effects of ATA (a nuclease inhibitor) on the direct transfection of macaque and murine lung tissue; compared the levels of DNase activity in murine, primate, and human lung fluids; and tested the inhibitory activity of ATA on the DNase activity present in these samples. Fluorescent microspheres were used to detect areas of transfection in lung.Results
Intratracheal administration of a nuclease inhibitor (ATA) with naked DNA (0.5 µg ATA/g body weight) enhanced direct transfection efficacy in macaque lung by over 86‐fold and by over 54‐fold in mouse lung. Hematoxylin and eosin staining showed no apparent tissue toxicity. Moreover, macaque, human, and mouse lung fluids were found to possess similar levels of DNase activity and this activity was inhibited by similar concentrations of ATA. The authors also successfully pioneered the use of carboxylate‐modified microsphere tracers to identify areas of transfection and/or treatment.Conclusion
This work provides evidence that using direct nuclease inhibitors will enhance lung transfection and that nuclease activity is present in all lung fluids tested, which can be inhibited by the use of direct DNase inhibitors. Copyright © 2002 John Wiley & Sons, Ltd.196.
The norbornenodiazetine plant growth regulator tetcyclacis, when applied to roots of Avena sativa, caused a substantial increase in the cholesterol content of the shoots. Amounts of the C-24 alkylated sterols campesterol, stigmasterol and sitosterol all declined. A similar alteration in the sterol profile was observed for a plasma membrane preparation from the shoots. Changes in the sterol composition of root tissue were much less pronounced. 相似文献
197.
Arla L. Bush Roger P. Wise 《Molecular breeding : new strategies in plant improvement》1998,4(1):13-21
The D526-derived BC1F2 population of hexaploid oat segregates for resistance to crown rust isolate 345. A mapping population consisting of 440 F2 individuals was used to develop a high-resolution RFLP map of the area of the genome where Pc71, the locus conferring this resistance, is located. Twelve RFLP markers have been identified within ca. 6 cM of Pc71, with cosegregating loci Xcdo1502 and Xcdo783 positioned 0.2 cM from the locus. All of the RFLP markers map to the same side of the locus, suggesting either that the Pc71 resistance locus resides at the end of a linkage group, or that there is no detectable heterozygosity on the opposite side. Some degree of microcollinearity between rice and oat is present in this area, as the two markers, Xcdo783 and Xrz69, linked to Pc71 are linked also in rice; however the genetic distance in oat is much less than the genetic distance in rice (6.2 cM and 20 cM, respectively). 相似文献
198.
Barley yellow dwarf virus (BYDV) causes significant losses in yield and in overwintering ability of winter cereals. Mechanisms by which the physiology of plants is affected by the virus are not clear. To see how carbohydrates in the crown of winter cereals were affected by BYDV, fructan isomers of degree of polymerization (DP) 3–5, fructan DP>6 and the simple sugars, glucose, fructose and sucrose, were measured before and during cold hardening in three oat ( Avena sativa L.) cultivars, 'Wintok', 'Coast Black' and 'Fulghum'. On a fresh weight basis fructan DP>6 decreased by 50% in infected 'Wintok' and 'Coast Black' and by 25% in 'Fulghum'. Two DP3, one DP4 and one DP5 isomer were significantly higher than non-infected controls. The percentages of simple sugars in infected crowns were significantly higher than controls in all three cultivars in every week except the first week of hardening. Crude enzyme extracts from BYDV infected plants incubated with sucrose suggested higher invertase and lower sucrose-sucrosyl transferase activity. When incubated with 1-kestose and neokestin, no significant difference was found in fructose fructosyl transferase or in hydrolase activity. The activity of unidentified enzymes catalysing the synthesis of larger (DP>5) fructan was altered by BYDV. The decrease of carbohydrates in the crown induced indirectly by BYDV may alter the plant's capacity to regenerate tillers in the spring. The ability of plants to prevent or tolerate carbohydrate fluctuations induced by BYDV infection may be an important genetically regulated characteristic for developing virus-resistant cultivars. 相似文献
199.
The absorption and translocation of fenoxaprop-ethyl and imazamethabenz-methyl were investigated in wild oat (Avena fatua L.) plants grown under different temperature and light intensity conditions by using 14C tracer techniques. The phytotoxicity of both herbicides, applied as individual droplets, was also determined under similar environments. The absorption of fenoxaprop-ethyl and imazamethabenz-methyl was increased by high temperature (30/20°C) and to a lesser extent by 70% shading; low temperature (10/5°C) had limited effect on the absorption. The basipetal translocation of fenoxaprop-ethyl was not affected by high temperature, and the increase in imazamethabenz-methyl translocation at high temperature was likely the result of the increased absorption. Low temperature decreased total translocation and translocation efficiency in both fenoxaprop-ethyl and imazamethabenz-methyl. Low light intensity tended to reduce the efficiency of basipetal translocation of both herbicides. Fenoxaprop-ethyl phytotoxicity was reduced by high temperature but not by low temperature. Temperature had little effect on imazamethabenz-methyl effectiveness. Under 70% shading, the phytotoxicity of both herbicides was enhanced.Abbreviation S.E.D.
standard errors of difference 相似文献
200.
Lothar Dunemann Nicolaus Von Wirén Rudolf Schulz Horst Marschner 《Plant and Soil》1991,133(2):263-269
Soil solutions were collected for speciation analysis of nickel from a pot experiment with oats. Oat plants (Avena sativa L.) were grown on 3 soils differing in total amount and origin of nickel (Ni) (Luvisol, LS with 28 mg kg-1; sludge amended Luvisol, LS+SS with 32 mg kg-1; Cambisol, CS with 95 mg kg-1). Results were compared with those for soil solutions obtained from corresponding unplanted pots. Separation methods were used for characterization of size and charge distribution and stability of the Ni species. In addition, short-term experiments were performed on the uptake rates of Ni by oat plants from the different soil solutions as well as from nutrient solutions with increasing concentrations of a synthetic chelator.The Ni concentrations in the soil solutions of unplanted soils increased in the order: LS5000 g mol-1) was the predominant form, whereas in the other soils the low-molecular-size cationic and chelated Ni species (500–1000 g mol-1) dominated in the soil solution. In the short-term uptake studies, the uptake rates of Ni from the solutions decreased in the order: nutrient solution > soil solutions, and in the latter in the order: LS>LS+SS>CS, which was inversely related to the concentrations of dissolved organic carbon in the soil solutions.The results demonstrate that Ni availability to plants is not only affected by total concentration of Ni in the soil solution and the rate of replenishment from the solid phase, but also by Ni species, which can differ considerably between soil types. 相似文献