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41.
The effect of temperature on membrane hydraulic conductivity 总被引:5,自引:0,他引:5
The objective of this study was to use the temperature dependence of water permeability to suggest the physical mechanisms of water transport across membranes of osmotically slowly responding cells and to demonstrate that insight into water transport mechanisms in these cells may be gained from easily performed experiments using an electronic particle counter. Osmotic responses of V-79W Chinese hamster fibroblast cells were measured in hypertonic solutions at various temperatures and the membrane hydraulic conductivity was determined. The results were fit with the general Arrhenius equation with two free parameters, and also fit with two specific membrane models each having only one free parameter. Data from the literature including that for human bone marrow stem cells, hamster pancreatic islets, and bovine articular cartilage chondrocytes were also examined. The results indicated that the membrane models could be used in conjunction with measured permeability data at different temperatures to investigate the method of water movement across various cell membranes. This approach for slower responding cells challenges the current concept that the presence of aqueous pores is always accompanied by an osmotic water permeability value, P(f)>0.01 cm/s. The possibility of water transport through aqueous pores in lower-permeability cells is proposed. 相似文献
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Thermally-induced fluctuations of individual phospholipids in a bilayer lipid membrane (BLM) are converted into collective motions due to the intermolecular interactions. Here, we demonstrate that transbilayer stochastic pores can be generated via collective thermal movements (CTM). Using the elastic theory of continuous media applied to smectic-A liquid crystals, we estimate the pore radius and the energetic requirements for pore appearance. Three types of thermally-induced transbilayer pores could be formed through BLMs: open and stable, open and unstable, and closed. In most of the situations, two open and stable pores with different radii could be generated. Notably, the two pores have the same generation probability. Unstable pores are possible to appear across thin bilayers that contain phospholipids with a large polar headgroup. Closed pores are present throughout the cases that we have inspected. The effects of hydrophobic thickness, polar headgroup size of phospholipids, temperature, surface tension, and elastic compression on the pore formation and pore stability have been examined as well. 相似文献
44.
Devising analysis tools for elucidating the regulatory mechanism of complex enzymes has been a challenging task for many decades. It generally requires the determination of the structural‐dynamical information of protein solvent systems far from equilibrium over multiple length and time scales, which is still difficult both theoretically and experimentally. To cope with the problem, we introduce a full‐residue space multiscale simulation method based on a combination of the kinetic Monte Carlo and molecular dynamics techniques, in which the rates of the rate‐determining processes are evaluated from a biomolecular forcefield on the fly during the simulation run by taking into account the full space of residues. To demonstrate its reliability and efficiency, we explore the light‐induced functional behavior of the full‐length phototropin1 from Chlamydomonas reinhardtii (Cr‐phot1) and its various subdomains. Our results demonstrate that in the dark state the light oxygen voltage‐2‐Jα (LOV2‐Jα) photoswitch inhibits the enzymatic activity of the kinase, whereas the LOV1‐Jα photoswitch controls the dimerization with the LOV2 domain. This leads to the repulsion of the LOV1‐LOV2 linker out of the interface region between both LOV domains, which results in a positively charged surface suitable for cell–membrane interaction. By contrast, in the light state, we observe that the distance between both LOV domains is increased and the LOV1‐LOV2 linker forms a helix–turn–helix (HTH) motif, which enables gene control through nucleotide binding. Finally, we find that the kinase is activated through the disruption of the Jα‐helix from the LOV2 domain, which is followed by a stretching of the activation loop (A‐loop) and broadening of the catalytic cleft of the kinase. Proteins 2014; 82:2018–2040. © 2014 Wiley Periodicals, Inc. 相似文献
45.
Abstract The behavior of mixtures of associating and non-associating chains confined in pores with activated surfaces is studied by means of molecular simulation. The fluid molecules are modeled as a chain of four tangent Lennard-Jones spheres. Some of the chains have an additional associating square-well site placed in an end sphere. The activated surfaces of the slit pore are modeled via an integrated Lennard-Jones (10-4-3) potential with specific association sites protruding from the surface. We present Gibbs ensemble Monte Carlo simulation results for the partitioning of mixtures of chains in the bulk and confined phases for this particular model. The chain-wall association governs the adsorption behavior of the system. The preferential adsorption of associating chains is seen to strongly depend on temperature and pore width. Selectivities obtained are in the range of those seen in experiments of alkane-alkanol mixtures. 相似文献
46.
Sulfur‐Impregnated,Sandwich‐Type,Hybrid Carbon Nanosheets with Hierarchical Porous Structure for High‐Performance Lithium‐Sulfur Batteries 下载免费PDF全文
Xi'an Chen Zhubing Xiao Xutao Ning Zheng Liu Zhi Yang Chao Zou Shun Wang Xiaohua Chen Ying Chen Shaoming Huang 《Liver Transplantation》2014,4(13)
Sandwich‐type hybrid carbon nanosheets (SCNMM) consisting of graphene and micro/mesoporous carbon layer are fabricated via a double template method using graphene oxide as the shape‐directing agent and SiO2 nanoparticles as the mesoporous guide. The polypyrrole synthesized in situ on the graphene oxide sheets is used as a carbon precursor. The micro/mesoporous strcutures of the SCNMM are created by a carbonization process followed by HF solution etching and KOH treatment. Sulfur is impregnated into the hybrid carbon nanosheets to generate S@SCNMM composites for the cathode materials in Li‐S secondary batteries. The microstructures and electrochemical performance of the as‐prepared samples are investigated in detail. The hybrid carbon nanosheets, which have a thickness of about 10–25 nm, high surface area of 1588 m2 g?1, and broad pore size distribution of 0.8–6.0 nm, are highly interconnected to form a 3D hierarchical structure. The S@SCNMM sample with the sulfur content of 74 wt% exhibits excellent electrochemical performance, including large reversible capacity, good cycling stability and coulombic efficiency, and good rate capability, which is believed to be due to the structure of hybrid carbon materials with hierarchical porous structure, which have large specific surface area and pore volume. 相似文献
47.
Scaling Theory: Application to Marine Ornithology 总被引:1,自引:0,他引:1
David C. Schneider 《Ecosystems》2002,5(8):0736-0748
The problem of scale has three components: (a) direct measurement is usually confined to small areas and brief periods, (b)
the most pressing issues occur at the scale of ecosystems and decades, but (c) direct scale-up fails when pattern and process
at small scales differ from those at larger scales. Recognition of this dilemma has grown exponentially since around 1980.
The problem of scale is particularly acute for seabirds, which inhabit one of the most extensive habitats on the planet—the
surface of the ocean. The application of power laws is a promising solution to the problem. Power laws have a long empirical
tradition, are readily estimated from data, and now have a theoretical basis. Power law behavior (with nonintegral exponents)
appears in systems with episodically warring exponential rates. In marine ornithology, examples of areas where power laws
can be applied include patchy spatial distributions, the association of predator with prey, the scaling of food intake to
body size, and fractal habitat structure. Scaling theory and power laws are applicable to a wide variety of ecosystem phenomena
and dynamics, including fluxes of material and energy.
Received 7 May 2001; accepted 12 April 2002. 相似文献
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49.
Eggs of Schistosoma mansoni, Schistosoma japonicum, Schistosoma haematobium, and Schistosoma mekongi incubated in serum from infected animals or humans had characteristic circumoval precipitin reaction products. When studied by transmission electron microscopy, these reaction products were seen in all species associated with egg shell pores from which antigen emerges. Reaction products were of variable density and sometimes were similar to long “septate” reaction products seen with light microscopy. No limiting membrane was seen around the reaction products. No true septa were seen. It is probable that the reaction product emerged from shell pores and was added to emerging material in spurts giving the septate appearance. No control eggs fixed immediately or first incubated in either saline or serum of uninfected controls showed characteristic reaction products. 相似文献
50.
Summary An examination of the mechanical interaction between elongating roots and rigid pores of precisely known size is reported. Sheets of steel mesh and glass capillaries were used as systems of rigid pores. The roots of grasses were found to be capable of penetrating pores much smaller than their nominal thickness, this capability being limited by the size of the root cap and the stele. Constricted root tips elongated at a slower rate but could grow down long capillaries if adequately aerated. The size of rigid pore critical to the growth of perennial ryegrass was 315 m. 相似文献