全文获取类型
收费全文 | 9698篇 |
免费 | 481篇 |
国内免费 | 836篇 |
专业分类
11015篇 |
出版年
2024年 | 13篇 |
2023年 | 120篇 |
2022年 | 188篇 |
2021年 | 203篇 |
2020年 | 182篇 |
2019年 | 180篇 |
2018年 | 209篇 |
2017年 | 155篇 |
2016年 | 214篇 |
2015年 | 220篇 |
2014年 | 467篇 |
2013年 | 583篇 |
2012年 | 383篇 |
2011年 | 383篇 |
2010年 | 324篇 |
2009年 | 425篇 |
2008年 | 474篇 |
2007年 | 458篇 |
2006年 | 467篇 |
2005年 | 468篇 |
2004年 | 423篇 |
2003年 | 428篇 |
2002年 | 413篇 |
2001年 | 331篇 |
2000年 | 265篇 |
1999年 | 271篇 |
1998年 | 254篇 |
1997年 | 243篇 |
1996年 | 212篇 |
1995年 | 221篇 |
1994年 | 206篇 |
1993年 | 193篇 |
1992年 | 226篇 |
1991年 | 156篇 |
1990年 | 142篇 |
1989年 | 129篇 |
1988年 | 135篇 |
1987年 | 104篇 |
1986年 | 96篇 |
1985年 | 100篇 |
1984年 | 110篇 |
1983年 | 59篇 |
1982年 | 74篇 |
1981年 | 36篇 |
1980年 | 17篇 |
1979年 | 18篇 |
1978年 | 10篇 |
1977年 | 10篇 |
1976年 | 8篇 |
1973年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 11 毫秒
61.
Summary Genomic clones encoding two Volvox -tubulin genes have been isolated and shown to represent the only two -tubulin genes in the genome. Restriction fragment length polymorphism analysis was used to demonstrate that the two genes are genetically linked. One of these genes was sequenced and the mRNA start site(s) determined by primer extension. A comparison of its sequence to those of the two -tubulin genes of Chlamydomonas revealed: (1) a high degree of conservation of the coding region, with the predicted amino acid sequence differing only in the C-terminal residue; (2) extensive sequence conservation in the 5 untranslated leader region and a 16 bp (putative regulatory) sequence in the promoter region; (3) the same number and location of introns, with a short region of homology in intron 1, but little significant homology in introns 2 and 3. 相似文献
62.
Cloning and sequencing of the genes encoding the large and the small subunits of the H2 uptake hydrogenase (hup) of Rhodobacter capsulatus 总被引:12,自引:0,他引:12
Michèle Leclerc Annette Colbeau Béatrice Cauvin Paulette M. Vignais 《Molecular & general genetics : MGG》1988,214(1):97-107
Summary The structural genes (hup) of the H2 uptake hydrogenase of Rhodobacter capsulatus were isolated from a cosmid gene library of R. capsulatus DNA by hybridization with the structural genes of the H2 uptake hydrogenase of Bradyrhizobium japonicum. The R. capsulatus genes were localized on a 3.5 kb HindIII fragment. The fragment, cloned onto plasmid pAC76, restored hydrogenase activity and autotrophic growth of the R. capsulatus mutant JP91, deficient in hydrogenase activity (Hup-). The nucleotide sequence, determined by the dideoxy chain termination method, revealed the presence of two open reading frames. The gene encoding the large subunit of hydrogenase (hupL) was identified from the size of its protein product (68108 dalton) and by alignment with the NH2 amino acid protein sequence determined by Edman degradation. Upstream and separated from the large subunit by only three nucleotides was a gene encoding a 34 256 dalton polypeptide. Its amino acid sequence showed 80% identity with the small subunit of the hydrogenase of B. japonicum. The gene was identified as the structural gene of the small subunit of R. capsulatus hydrogenase (hupS). The R. capsulatus hydrogenase also showed homology, but to a lesser extent, with the hydrogenase of Desulfovibrio baculatus and D. gigas. In the R. capsulatus hydrogenase the Cys residues, (13 in the small subunit and 12 in the large subunit) were not arranged in the typical configuration found in [4Fe–4S] ferredoxins. 相似文献
63.
Identification, cloning, nucleotide sequence and chromosomal map location of hns, the structural gene for Escherichia coli DNA-binding protein H-NS 总被引:10,自引:0,他引:10
Cynthia L. Pon Raffaele A. Calogero Claudio O. Gualerzi 《Molecular & general genetics : MGG》1988,212(2):199-202
Summary Beginning with a synthetic oligonucleotide probe derived from its amino acid sequence, we have identified, cloned and sequenced the hns gene encoding H-NS, an abundant Escherichia coli 15 kDa DNA-binding protein with a possible histone-like function. The amino acid sequence of the protein deduced from the nucleotide sequence is in full agreement with that determined for H-NS. By comparison of the restriction map of the cloned gene and of its neighboring regions with the physical map of E. coli K12 as well as by hybridization of the hns gene with restriction fragments derived from the total chromosome, we have located the hns gene oriented counterclockwise at 6.1 min on the E. coli chromosome, just before an IS30 insertion element. 相似文献
64.
Hiro Nakamura Hiroshi Murakami Ichiro Yamato Yasuhiro Anraku 《Molecular & general genetics : MGG》1988,212(1):1-5
Summary The complete nucleotide sequence of the Escherichia coli cybB gene for diheme cytochrome b
561 and its flanking region was determined. The cybB gene comprises 525 nucleotides and encodes a 175 amino acid polypeptide with a molecular weight of 20160. From its deduced amino acid sequence, cytochrome b
561 is predicted to be very hydrophobic (polarity 33.7%) and to have three membrane spanning regions. Histidines, canonical ligand residues for protohemes, are localized in these regions, and the heme pockets are thought to be in the cytoplasmic membrane. No significant homology of the primary structure of cytochrome b
561 with those of other bacterial b-type cytochromes was observed. 相似文献
65.
R. Chima Wokocha 《Plant and Soil》1988,106(1):146-148
Populations of viable sclerotia ofSclerotium rolfsii were highest in soil in a field in which tomato was planted for three successive years before sampling and in one in which
tomato followed groundnut in the 2 years prior to this study. The lowest sclerotial numbers were recorded in fields in which
groundnut followed maize or in which maize or sorghum was the last crop before sampling. 相似文献
66.
67.
David K. Grandy Richard Leduc Haripriya Makam Thomas Flanagan Emanuel J. Diliberto Jr. Olivier Civelli O. Humberto Viveros 《Cellular and molecular neurobiology》1992,12(2):185-192
1. A novel 1745-dalton pyroglutamyl peptide (BAM-1745)6 was recently isolated and characterized from bovine adrenal medulla chromaffin granules. Its amino acid sequence was found to be 93% identical to residues 580-593 of human chromogranin B (secretogranin I). 2. Based on this sequence a degenerate oligonucleotide probe was synthesized and used to identify a 2.4-kb bovine adrenal medulla chromogranin B cDNA. 3. The deduced polypeptide is 647 amino acids long and begins with a putative signal sequence of 20 residues as in the human, rat, and mouse proteins. Also conserved in the bovine protein is a tyrosine residue which may be sulfated, two N-terminal cysteines, and many paired basic amino acids which may serve as sites of posttranslational processing. The peptide BAM-1745 is flanked by paired basic amino acids and therefore is most likely a product of posttranslational processing. Bovine chromogranin B is 67, 58, and 58% identical to the human, rat, and mouse chromogranin B proteins, respectively. 4. The carboxyl terminus of bovine chromogranin B, including BAM-1745, was found to be the most conserved region of the polypeptide and may identify it as an important functional domain. 相似文献
68.
Bruce R. Johnson Jack H. Peck Ronald M. Harris-Warrick 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1992,170(2):201-209
Summary The anterior burster neuron of the lobster (Panulirus interruptus) stomatogastric ganglion is a conditional burster that functions as the primary pacemaker for the pyloric motor network. When modulatory inputs to this cell are blocked, it loses its bursting properties and becomes quiescent. Applications of the monoamines, dopamine, octopamine or serotonin restore rhythmic bursting in this cell (Flamm and Harris-Warrick 1986). At 15 °C, serotonin- and octopamine-induced oscillations depend critically upon sodium entry (blocked by low sodium saline or tetrodotoxin); dopamine-induced oscillations depend upon calcium entry (blocked by reduced extracellular calcium; Harris-Warrick and Flamm 1987). We show here that the ionic dependence of amine-induced oscillations in the anterior burster cell differs at 15 and 21 °C. At 21 °C, all amines have the potential to induce rhythmic oscillations in saline containing tetrodotoxin. At the elevated temperature and in tetrodotoxin, both calcium and sodium currents are essential for the maintenance of dopamine-induced oscillaions; serotonin-induced oscillations do not depend upon either calcium or sodium alone; octopamine-induced oscillations do not depend upon calcium and show a variable dependence upon sodium. Thus, multiple ionic mechanisms, which vary with both the modulator and the ambient temperature, can be recruited to support rhythmic activity in a conditional burster neuron.Abbreviations
AB
anterior burster
-
PD
pyloric dilator
-
PY
pyloric constrictor
-
DA
dopamine
-
5HT
serotonin
-
Oct
octopamine
-
STG
stomatogastric ganglion
-
TTX
tetrodotoxin
-
GSP
graded synaptic potential 相似文献
69.
70.
Complete nucleotide sequence of the mitochondrial DNA from a liverwort,Marchantia polymorpha 总被引:1,自引:1,他引:0
Kenji Oda Katsuyuki Yamato Eiji Ohta Yasukazu Nakamura Miho Takemura Naoko Nozato Kinya Akashi Takeshi Kanegae Yutaka Ogura Takayuki Kohchi Kanji Ohyama 《Plant Molecular Biology Reporter》1992,10(2):105-163
Libraries of cosmid and plasmid clones covering the entire region of mtDNA from the liverwortMarchantia polymorpha were constructed. These clones were used for the determination of the complete nucleotide sequence of the liverwort mtDNA
totally 186,608 bp (GenBank no. M68929) and including genes for 3 species of ribosomal RNAs, 29 genes for 27 species of transfer
RNAs, and 30 genes for functionally known proteins (16 ribosomal proteins, 3 subunits of cytochromec oxidase, apocytochromeb protein, 3 subunits of H+-ATPase, and 7 subunits of NADH ubiquinone oxidoreductase). The genome also contains 32 unidentified open reading frames.
Thus the complete nucleotide sequences from both chloroplast and mitochondrial genomes have been determined in the same organism.
Plasmid clones are available upon the request.
Gene names are represented according to Lonsdale and Leaver (1988) with modifications recommended by Lonsdale (personal communication). 相似文献