南亚热带气候下三种树木径向生长季节节律研究

用带状测树器对南亚热带气候条件下的落叶树柚木（Ｔｅｃｔｏｎａ ｇｒａｎｄｉｓ Ｌ．ｆ．）、喜树（Ｃａｍｐｔｏｔｈｅｃａ ａｃｕｍｉｎａｔａ Ｄｅｃｎｅ）和常绿树火烧花（Ｍａｙｏｄｅｎｄｒｏｎ ｉｇｅｕｍ Ｋｕｒｚ）１６年生（测定开始时年龄）的立木的径向生长进行了６ａ的测定。研究结果表明,所测立木的年均胸围生长量在１．５～２．５ｃｍ（胸径生长量为０．４８～０．８０ｃｍ）之间,年间有一定的差异。年生长     

Long-term results of evaporation rate in xerothermic Oak altitudinal vegetation stage in Southern Slovakia

The paper presents the results of the model computation of actual and potential evapotranspiration as well as evaporation measurements from the GGI-3000 Pan (evaporimeter) at 3 selected stations of Slovakia for the periods 1971–2000 (2 stations) and 1986–2000 (1 station). The localities are situated in the most southern parts of Slovakia in 1^st Oak stage and on southern slopes of the Carpathian promontories. A model computation of the monthly totals of actual and potential evapotranspiration was performed by a common solution of energy and water balance equations of the top one-meter layer of the soil. The model outputs are compared to the measurement data. The dependence of the ratio of actual evapotranspiration/ evaporation measured by the GGI-3000 Pan (E/E _p) on relative evapotranspiration (E/E _o), when E, E _o are calculated, is linear with a high correlation coefficient during the season from April to October. The paper also gives the comparison results of the mean monthly and annual air temperatures, temperatures of active and saturated surface with the mean monthly temperature of free water surface in the GGI-3000 Pan at these 3 stations (Bratislava-Koliba, Somotor and Žihárec). It was found that the mean free water surface temperature for the period from April to October is close to the mean temperature of saturated surface during that period. The mean temperatures of free water surface in the GGI-3000 Pan from April to October tend to increase for the period 1971–2000.     

Meta-basic estimates the size of druggable human genome

We present here the estimation of the upper limit of the number of molecular targets in the human genome that represent an opportunity for further therapeutic treatment. We select around ∼6300 human proteins that are similar to sequences of known protein targets collected from DrugBank database. Our bioinformatics study estimates the size of ‘druggable’ human genome to be around 20% of human proteome, i.e. the number of the possible protein targets for small-molecule drug design in medicinal chemistry. We do not take into account any toxicity prediction, the three-dimensional characteristics of the active site in the predicted ‘druggable’ protein families, or detailed chemical analysis of known inhibitors/drugs. Instead we rely on remote homology detection method Meta-BASIC, which is based on sequence and structural similarity. The prepared dataset of all predicted protein targets from human genome presents the unique opportunity for developing and benchmarking various in silico chemo/bio-informatics methods in the context of the virtual high throughput screening.     

A surgically confirmed case of breast sparganosis showing characteristic mammography and ultrasonography findings

A case of breast sparganosis was confirmed by surgical excision of a worm (fragmented into 5 pieces) in a 59-year-old Korean woman suffering from a palpable mass in the left breast. Mammography and ultrasonography characteristically revealed the presence of several well-defined, isodense and hypoechoic tubular masses, in the upper quadrant of the left breast, each mass consisting of a continuous cord- or worm-like structure. During surgery, a long segment of an actively moving sparganum of Spirometra sp. and 4 small fragments of the same worm, giving a total length of 20.3 cm, were extracted from the upper outer quadrant of the left breast and the axillary region. The infection source remains unclear, because the patient denied ingesting any snake or frog meat or drinking untreated water.     

Three clinical cases of cutaneous larva migrans

Three cases of cutaneous larva migrans (CLM) were diagnosed in a returnee from a trip to Thailand and in 2 domestic farmers during July and September, 2003. The linear and serpiginous skin lesions on the lower extremities were presented in all 3 cases. Routine laboratory findings were normal. In the imported case, a 650 x 30 micrometer sized filariform nematode larva, presumably a species of hookworm, was detected in the lesion. All cases were treated with 400 mg albendazole once daily for 3-5 days, and their skin lesions gradually improved. In the present study, a causative agent of CLM was isolated for the first time in the Republic of Korea. Moreover, we speculate that CLM is prevalent in farmers who are in frequent contact with soil in the Republic of Korea.     

Peripheral gangrene in patients with severe falciparum malaria: report of 3 cases

Peripheral gangrene, characterized by distal ischemia of the extremities, is a rare complication in patients with falciparum malaria. Patients with this complication have generally undergone early amputation of the affected areas. In this report, we describe 3 adult Thai patients presented at the Hospital for Tropical Diseases, Bangkok, with high grade of fever ranged 6-9 days, jaundice, acute renal failure, respiratory failure, alteration of consciousness and shock. Two patients had gangrene developed at the lower extremities on day 1 of hospitalization and 1 patient had gangrene developed on day 3. Blood smears revealed hyperparasitemia with Plasmodium falciparum. These patients were diagnosed as having severe malaria with peripheral gangrene. The resolution of gangrene was successfully achieved by treatment with artesunate and conservative treatment in 2 of 3 cases.     

利用Red重组工程技术解决外源基因在大肠杆菌染色体lac操纵子中的表达

为了应用Red重组工程技术实现外源基因在大肠杆菌染色体上的表达, 寻找染色体上外源蛋白的稳定高效表达位点, 使用Red重组工程系统和kan/sacB无痕迹修饰技术, 将易于定量分析的荧光素酶报告基因替换DY330染色体lac操纵子中的lacZ基因。检测该位点的表达效率结果显示: 大肠杆菌染色体上lac操纵子能够高效稳定表达外源基因, 初步证明了染色体可以作为外源蛋白或抗原的表达载体, 不会影响细菌的生长繁殖。     

利用GIS和多变量分析估算青藏高原月降水

随着空间降水信息需求的日益增加,空间降水插值已被广泛应用。降水区域不同,插值方法不同;时间尺度不同,插值方法也不相同。适合于所有地区的通用降水插值模型是不存在的。青藏高原自然地理特征独特,分析高原降水的时空格局意义重要。以青藏高原及其周边地区140个气象站点的月降水信息及其该地区的数字高程数据(DEM)为基础,利用G IS工具,对比分析了五种插值方法在青藏高原不同降水年份(以1998年、1997年分别代表丰水及欠水年份)的干湿季(1998年的干湿季分别以12月份和8月份为代表,1997年的干湿季分别以1月份和7月份为代表)月降水插值中的应用,并对整个高原地区的干季和湿季的月降水进行制图。这5种插值方法分别是:克里金插值法、反距离加权法、样条法、混合插值法Ⅰ和混合插值法Ⅱ,前3种插值方法未考虑海拔高度对降水的影响,而混合插值法则将高程作为降水的重要影响因子。结果表明:①在干季,无论是丰水还是欠水年份,月降水量都比较少,高程对降水量的影响较小,在月降水插值时可不考虑高程的影响,克里金法的月降水插值精度最高。②在湿季,月降水量较多,高程的影响较大,混合插值法比局部插值法及克里金插值法的精度高,尤以混合插值法Ⅱ(多元回归和样条法的综合)的精度最高。③干季,整个高原的月降水很少,西部和北部降水最少,东部和南部相对较多;湿季,高原的月降水较多,空间格局表现为由东南向西北递减。