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101.
Genotypic variation in seedling root architectural traits and implications for drought adaptation in wheat (Triticum aestivum L.) 总被引:1,自引:0,他引:1
Ahmad M. Manschadi Graeme L. Hammer John T. Christopher Peter deVoil 《Plant and Soil》2008,303(1-2):115-129
Root system characteristics are of fundamental importance to soil exploration and below-ground resource acquisition. Root
architectural traits determine the in situ space-filling properties of a root system or root architecture. The growth angle
of root axes is a principal component of root system architecture that has been strongly associated with acquisition efficiency
in many crop species. The aims of this study were to examine the extent of genotypic variability for the growth angle and
number of seminal roots in 27 current Australian and 3 CIMMYT wheat (Triticum aestivum L.) genotypes, and to quantify using fractal analysis the root system architecture of a subset of wheat genotypes contrasting
in drought tolerance and seminal root characteristics. The growth angle and number of seminal roots showed significant genotypic
variation among the wheat genotypes with values ranging from 36 to 56 (degrees) and 3 to 5 (plant−1), respectively. Cluster analysis of wheat genotypes based on similarity in their seminal root characteristics resulted in
four groups. The group composition reflected to some extent the genetic background and environmental adaptation of genotypes.
Wheat cultivars grown widely in the Mediterranean environments of southern and western Australia generally had wider growth
angle and lower number of seminal axes. In contrast, cultivars with superior performance on deep clay soils in the northern
cropping region, such as SeriM82, Baxter, Babax, and Dharwar Dry exhibited a narrower angle of seminal axes. The wheat genotypes
also showed significant variation in fractal dimension (D). The D values calculated for the individual segments of each root system suggested that, compared to the standard cultivar Hartog,
the drought-tolerant genotypes adapted to the northern region tended to distribute relatively more roots in the soil volume
directly underneath the plant. These findings suggest that wheat root system architecture is closely linked to the angle of
seminal root axes at the seedling stage. The implications of genotypic variation in the seminal root characteristics and fractal
dimension for specific adaptation to drought environment types are discussed with emphasis on the possible exploitation of
root architectural traits in breeding for improved wheat cultivars for water-limited environments. 相似文献
102.
103.
Habitat structure, the physical arrangement of objects in space, affects animal populations, ecological interactions, species diversity, and ecosystem functioning. Conventional forest management practices that reduce habitat structural complexity are thus under reappraisal, and there are increasing efforts to understand how habitat structure affects functionally important species. We investigate here the effect of habitat structures on small mammal dens and their interactions with widespread antagonistic species (wild boars). Among habitat structures we considered tree dimension (diameter), shrub cover, rock cover, dead wood volume, and stump area. Ground cover with rooting signs measured wild boar disturbance. The number of small mammal dens was related positively to rock cover and tree diameter, and negatively to wild boar disturbance. Additionally, a positive interaction between wild boar disturbance and tree diameter emerged, showing that the effect of big trees on small mammal nesting was more evident with higher levels of disturbance. This study suggests that habitat structures that can be affected by forest management provide effective refuges for functionally important species. The effect of habitat structure on species survival and interactions thus needs to be addressed to understand ecosystem management and functioning. 相似文献
104.
Chang-Gi Kim Dae In Kim Hyo-Jeong Kim Ji Eun Park Bumkyu Lee Kee Woong Park Soon-Chun Jeong Kyung Hwa Choi Joo Hee An Kang-Hyun Cho Young Soon Kim Hwan Mook Kim 《Journal of Plant Biology》2009,52(3):251-258
We conducted a 2-year field assessment of the gene flow from genetically modified (GM) chili pepper (Capsicum annuum L.), containing the PepEST (pepper esterase) gene, to a non-GM control line “WT512” and two commercial hybrid cultivars, “Manidda” and “Cheongpung Myeongwol
(CM).” After seeds were collected from the pollen-recipient non-GM plants, hybrids between them and the GM peppers were screened
by a hygromycin assay. PCR with the targeting hpt gene was performed to confirm the presence of transgenes in hygromycin-resistant seedlings. Out of 7,071 “WT512” seeds and
6,854 “Manidda” seeds collected in 2006, eight and 12 hybrids, respectively, were detected. In 2007, 33 hybrids from 3,456
“WT512” seeds and 50 hybrids from 3,457 “CM” seeds were found. The highest frequency of gene flow, 6.19%, was observed in
that 2007 trial. These results suggest that a limited isolation distance would be sufficient to prevent gene flow from GM
to conventionally bred chili peppers. 相似文献
105.
Elisa Marchetti Sara Alberghini Andrea Battisti Andrea Squartini Piero Baronio 《Biocontrol Science and Technology》2009,19(5):463-473
The Cry9Aa entomocidal toxin from Bacillus thuringiensis ssp. galleriae (Btg) and an epiphytic Pseudomonas sp. derivative carrying the cloned cry9Aa gene from Btg are active against the pine processionary moth Thaumetopoea pityocampa and the laboratory model species Galleria mellonella. A laboratory study was conducted to investigate the side effects of the Cry9Aa toxin and the engineered bacterium on the post-embryonic development of Exorista larvarum, a larval parasitoid of forest lepidopterous defoliators, cultured in the factitious host G. mellonella. In a first experiment, the purified toxin and the commercial Bt preparation Foray 48B induced a mortality of G. mellonella sixth-instar larvae significantly higher than that of the distilled water control. In parallel, the development of E. larvarum in this host was assessed, but no significant difference was found for any of the parasitoid parameters examined (i.e., eggs oviposited, percentage of puparia and adults and puparial weights). In subsequent experiments, cry9Aa-Pseudomonas suspension significantly increased the mortality of sixth instar G. mellonella larvae compared to untransformed Pseudomonas sp. suspension and distilled water. As to the parasitoid parameters, the cry9Aa-Pseudomonas did not significantly affect the number of oviposited eggs, percentage of puparia and puparial weights. It can be concluded that the post-embryonic development of E. larvarum was not affected by host treatment with either Cry9Aa toxin or cry9Aa-Pseudomonas under the laboratory conditions tested. Although direct effects on parasitoid performance have not been shown, indirect effects could still occur and need to be considered in future studies concerning the effects of genetically modified Bt-derivatives. 相似文献
106.
Achromobacter xylosoxidans is known to utilize d-glucose via the modified Entner-Doudoroff pathway. Although d-gluconate dehydratase produced from this bacterium was purified and partially characterized previously, a gene that encodes
this enzyme has not yet been identified. To obtain protein information on bacterial d-gluconate dehydratase, we partially purified d-gluconate dehydratase in A. xylosoxidans and investigated its biochemical properties. Two degenerate primers were designed based on the N-terminal amino acid sequence
of the partially purified d-gluconate dehydratase. Through PCR performed using degenerate primers, a 1,782-bp DNA sequence encoding the A. xylosoxidans
d-gluconate dehydratase (gnaD) was obtained. The deduced amino acid sequence of A. xylosoxidans gnaD showed strong similarity with that of proteins belonging to the dihydroxy-acid dehydratase/phosphogluconate dehydratase family
(COG0129). This is in contrast to the archaeal d-gluconate dehydratase, which belongs to the enolase superfamily (COG4948). The phylogenetic tree showed that A. xylosoxidans
d-gluconate dehydratase is closer to the 6-phosphogluconate dehydratase than the dihydroxy-acid dehydratase. Interestingly,
a clade containing A. xylosoxidans enzyme was clustered with proteins annotated as a second and a third dihydroxy-acid dehydratase in the genomes of Clostridium acetobutylicum (Cac_ilvD2) and Streptomyces ceolicolor (Sco_ilvD2, Sco_ilvD3), indicating that the function of these enzymes is the dehydration of d-gluconate. 相似文献
107.
顽拗植物龙眼基因组DNA提取方法的研究 总被引:20,自引:2,他引:20
为从顽拗植物龙眼(Dimocarpus longan Lour.)叶片中获得可供后续分子生物学操作的基因组DNA.针对其组织细胞内富含多酚、多糖、单宁及色素等物质的特点,采用改进的CTAB法和SDS法,即在核裂解之前先破碎细胞.将存在于细胞质中的次生物质去除后再裂解细胞桉.结合其它一些改进措施.提取到的DNA沉淀呈纯白色.极易溶解于TE中。两种改进方法的OD260和OD280比值分别达到1.82和1.73,其鲜叶基因组DNA产量分别为103ug/g和127ug/g:RAPD扩增条带清晰,丰富.完全满足后续分子生物学操作的要求,其中改良CTAB方法效果更为理想,与之埘比.传统的CTAB法和SDS法提取到的DNA沉淀呈浅黄色甚至红褐色,很难被TE溶解,其OD260和OD280比值均低于1.5,也得不到扩增产物。 相似文献
108.
B.S. Griffiths S. Caul J. Thompson A.N.E. Birch C. Scrimgeour M.N. Andersen J. Cortet A. Messéan C. Sausse B. Lacroix P.H. Krogh 《Plant and Soil》2005,275(1-2):135-146
Field trials were established at three European sites (Denmark, Eastern France, South-West France) of genetically modified
maize (Zea mays L.) expressing the CryIAb Bacillus thuringiensis toxin (Bt), the near-isogenic non-Bt cultivar, another conventional maize cultivar and grass. Soil from Denmark was sampled
at sowing (May) and harvest (October) over two years (2002, 2003); from E France at harvest 2002, sowing and harvest 2003;
and from SW France at sowing and harvest 2003. Samples were analysed for microbial community structure (2003 samples only)
by community-level physiological-profiling (CLPP) and phospholipid fatty acid analysis (PLFA), and protozoa and nematodes
in all samples. Individual differences within a site resulted from: greater nematode numbers under grass than maize on three
occasions; different nematode populations under the conventional maize cultivars once; and two occasions when there was a
reduced protozoan population under Bt maize compared to non-Bt maize. Microbial community structure within the sites only
varied with grass compared to maize, with one occurrence of CLPP varying between maize cultivars (Bt versus a conventional
cultivar). An overall comparison of Bt versus non-Bt maize across all three sites only revealed differences for nematodes,
with a smaller population under the Bt maize. Nematode community structure was different at each site and the Bt effect was
not confined to specific nematode taxa. The effect of the Bt maize was small and within the normal variation expected in these
agricultural systems. 相似文献
109.
Moiseeva OV Belova OV Solyanikova IP Schlömann M Golovleva LA 《Biochemistry. Biokhimii?a》2001,66(5):548-555
Chlorocatechol 1,2-dioxygenase (CC 1,2-DO), chloromuconate cycloisomerase (CMCI), chloromuconolactone isomerase (CMLI), and dienolactone hydrolase (DELH), the key enzymes of a new modified ortho-pathway in Rhodococcus opacus 1CP cells utilizing 2-chlorophenol via a 3-chlorocatechol branch of a modified ortho-pathway, were isolated and characterized. CC 1,2-DO showed the maximum activity with 3-chlorocatechol; its activity with catechol and 4-chlorocatechol was 93 and 50%, respectively. The enzyme of the studied pathway had physicochemical properties intermediate between the pyrocatechase of ordinary and chlorocatechase of modified pathways described earlier for this strain. In contrast to the enzymes investigated earlier, CMCI of the new pathway exhibited high substrate specificity. The enzyme had K
m for 2-chloromuconate of 142.86 M, V
max = 71.43 U/mg, pH optimum around 6.0, and temperature optimum at 65°C. CMCI converted 2-chloromuconate into 5-chloromuconolactone. CMLI converted 5-chloromuconolactone into cis-dienolactone used as a substrate by DELH; this enzyme did not convert trans-dienolactone. DELH had Km for cis-dienolactone of 200 M, V
max = 167 U/mg, pH optimum of 8.6, and temperature optimum of 40°C. These results confirm the existence of a new modified ortho-pathway for utilization of 2-chlorophenol by R. opacus 1CP. 相似文献
110.
pH-dependent conformational changes of ferricytochrome c induced by electrode surface microstructure
pH-dependent processes of bovine heart ferricytochrome c have been investigated by electronic absorption and circular dichroism (CD) spectra at functionalized single-wall carbon nanotubes (SWNTs) modified glass carbon electrode (SWNTs/GCE) using a long optical path thin layer cell. These methods enabled the pH-dependent conformational changes arising from the heme structure change to be monitored. The spectra obtained at functionalized SWNTs/GCE reflect electrode surface microstructure-dependent changes for pH-induced protein conformation, pKa of alkaline transition and structural microenvironment of the ferricytochrome c heme. pH-dependent conformational distribution curves of ferricytochrome c obtained by analysis of in situ CD spectra using singular value decomposition least square (SVDLS) method show that the functionalized SWNTs can retain native conformational stability of ferricytochrome c during alkaline transition. 相似文献