全文获取类型
收费全文 | 19634篇 |
免费 | 1881篇 |
国内免费 | 2637篇 |
出版年
2024年 | 105篇 |
2023年 | 446篇 |
2022年 | 521篇 |
2021年 | 670篇 |
2020年 | 744篇 |
2019年 | 893篇 |
2018年 | 741篇 |
2017年 | 887篇 |
2016年 | 847篇 |
2015年 | 813篇 |
2014年 | 1056篇 |
2013年 | 1552篇 |
2012年 | 848篇 |
2011年 | 1057篇 |
2010年 | 873篇 |
2009年 | 1107篇 |
2008年 | 1205篇 |
2007年 | 1125篇 |
2006年 | 1041篇 |
2005年 | 839篇 |
2004年 | 776篇 |
2003年 | 672篇 |
2002年 | 555篇 |
2001年 | 511篇 |
2000年 | 507篇 |
1999年 | 446篇 |
1998年 | 337篇 |
1997年 | 313篇 |
1996年 | 276篇 |
1995年 | 254篇 |
1994年 | 250篇 |
1993年 | 213篇 |
1992年 | 196篇 |
1991年 | 185篇 |
1990年 | 150篇 |
1989年 | 132篇 |
1988年 | 111篇 |
1987年 | 115篇 |
1986年 | 120篇 |
1985年 | 78篇 |
1984年 | 97篇 |
1983年 | 74篇 |
1982年 | 110篇 |
1981年 | 67篇 |
1980年 | 76篇 |
1979年 | 41篇 |
1978年 | 43篇 |
1977年 | 19篇 |
1976年 | 18篇 |
1974年 | 12篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
91.
Cannibalism as a life boat mechanism 总被引:2,自引:0,他引:2
Under certain conditions a cannibalistic population can survive when food for the adults is too scarce to support a non-cannibalistic population. Cannibalism can have this lifeboat effect if (i) the juveniles feed on a resource inaccessible to the adults; and (ii) the adults are cannibalistic and thus incorporate indirectly the inaccessible resource. Using a simple model we conclude that the mechanism works when, at low population densities, the average yield, in terms of new offspring, due to the energy provided by one cannibalized juvenile is larger than one. 相似文献
92.
93.
Ronald E. Koes Cornelis E. Spelt Jos N. M. Mol Anton G. M. Gerats 《Plant molecular biology》1988,10(4):375-385
Chalcone synthase (CHS) genes in Petunia hybrida comprise a multigene family containing at least 7 complete members in the strain Violet 30 (V30). Based on a high sequence homology in both coding and non-coding sequence, a number of CHS genes can be placed into two subfamilies. By restriction fragment length polymorphism (RFLP) analysis it was shown that both chromosomes II and V carry one of these subfamilies, in addition to the other CHS genes identified so far. Members of a subfamily were found to be closely linked genetically. Analysis of the Petunia species that contributed to the hybrid nature of P. hybrida (P. axillaris, P. parodii, P. inflata and P. violacea) shows that none of the CHS gene clusters is specific for either one of the parents and therefore did not arise as a consequence of the hybridization. The number of CHS genes within a subfamily varies considerably among these Petunia species. From this we infer that the CHS subfamilies arose from very recent gene duplications. 相似文献
94.
P. M. Abuja M. Schmuck I. Pilz P. Tomme M. Claeyssens H. Esterbauer 《European biophysics journal : EBJ》1988,15(6):339-342
Limited proteolysis (papain) of the cellobiohydrolase I (CBH I, 65 kDa) from Trichoderma reesei led to the seperation of two functional domains: a core protein (55 kDa) containing the active site, and a C-terminal glycopeptide (10 kDa) implicated in binding to the insoluble matrix (cellulose). The quaternary structures of the intact CBH I and its core in solution are now compared by small angle X-ray scattering (SAXS) measurements. The molecular parameters derived for the core (Rg=2.09 nm, Dmax=6.5 nm) and for the intact enzyme (Rg=4.27 nm, Dmax=18 nm) indicate very different shapes. The resulting models show a tadpole-like structure for the intact enzyme where the isotropic part coincides with the core protein and the flexible tail part should be identified with the C-terminal glycopeptide. Thus in this enzyme, functional differentiation is reflected in structural peculiarities.Abbreviations SAXS
small angle X-ray scattering
- SDS-PAGE
SDS-polyacrylamide gel electrophoresis
- IEF-PAG
polyacrylamide gel isoelectric focusing; cellobiohydrolase (CBH, 1,4--glucan cellobio hydrolase (E.C.3.2.1.91))
- Dmax
maximum diameter
- Rg
radius of gyration 相似文献
95.
A model is proposed for the effect of gramicidin A on the order and structure of phospholipid dispersions. According to this model, the addition of gramicidin A influences the surrounding lipids via two independent mechanisms. The first arises from a drop in surface pressure for those lipids substantially bounded by gramicidin A. The second mechanism arises from the increase in the phospholipid headgroup spacing due to the small polar region of the polypeptide. The model provides an explanation for the currently available NMR, X-ray diffraction and Langmuir monolayer results. The model also suggests mechanisms for the ability of gramicidin A to trigger a transition of the lipid from the lamellar to hexagonal II phase, the dependence of this transition on the lipid chain length and the formation of a lamellar phase with lysophosphatidylcholine.Abbreviations NMR
nuclear magnetic resonance
- DMPC
dimyristoylphosphatidylcholine
- S
molecular order parameter
- CSA
chemical shift anisotropy
- DPPC
dipalmitoylphosphati-dylcholine
- LPC
lysophosphatidylcholine 相似文献
96.
Data on the physico-chemical properties of proteins from soybean, groundnut, sesame seed, sunflower seed, safflower seed,
mustard seed, rapeseed and cotton seed are fairly extensive. An examination of the available data on high molecular weight
proteins suggests that there are similarities in many of their properties. In this report the similarity in amino acid composition,
size and shape, molecular weight, secondary structure, subunit composition, association-dissociation at high and low pH, stability
towards denaturants, hydrolysis by enzymes and quaternary structure of the high molecular weight proteins is discussed. Based
on these similarities a model has been proposed for the associationdissociation, denaturation and reassociation behaviour
of the high molecular weight proteins of oilseeds. 相似文献
97.
A soil nitrogen model was used for a 4-year simulation of nitrogen dynamics and nitrate leaching, both during grass ley growth
and after ploughing a grass ley. Model results were compared with field measurements of soil mineral-N status and leaching.
A soil water and heat model provided daily values for abiotic conditions, which were used as driving variables in the nitrogen
simulation.
Simulated values for mineral-N levels in the soil agreed well with field data for the first 3 years of the simulation. During
the final year the model predicted considerably higher levels of soil mineral-N content compared with measurements. To reach
the mineral-N level measured at the time of ploughing the ley, the simulated N-uptake by plants had to be increased by 8 g
N m−2.
Simulations of nitrate leaching suggested that estimates of leaching based on measurements in tile-drained plots can be considerably
underestimated. Accurate quantification of leaching in tile-drained plots often requires additional information on water-flow
paths.
A substantial increase in simulated and measured values for the mineral-N content of the soil occurred after ploughing the
ley. In the simulation, most of the increase was due to a high crop residue input and the absence of a growing crop after
ploughing. Litter accumulations in the soil during the 4-year period contributed little to the increase in soil mineral-N. 相似文献
98.
99.
J. Bastow Wilson 《Plant and Soil》1988,109(1):128-130
In two experiments, wheat plants growing in solutions of different nitrogen concentration were subjected to root pruning. In higher concentrations of nitrogen the growth rate was higher, and the proportional allocation of growth to shoot higher, but pruning did not affect the allocation of growth at either level of nitrogen. This result gives no support to Thornley's source-sink model of the control of shoot: root ratio. 相似文献
100.
J. S. Beckmann M. Soller 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1988,76(2):228-236
Summary By making use of pedigree information and information on marker-genotypes of the parent and F-1 individuals crossed to form an F-2 population, it is possible to carry out a linkage analysis between marker loci and loci affecting quantitative traits in a cross between segregating parent populations that are at fixation for alternative alleles at the QTL, but share the same alleles at the marker loci. For two-allele systems, depending on marker allele frequencies in the parent populations, 2–4 times as many F-2 offspring will have to be raised and scored for markers and quantitative traits in order to provide power equivalent to that obtained in a cross between fully inbred lines. Major savings in number of F-2 offspring raised can be achieved by scoring each parent pair for a large number of markers in each chromosomal region and scoring F-1 and F-2 offspring only for those markers for which the parents were homozygous for alternative alleles. For multiple allele systems, particularly when dealing with hypervariable loci, only 10%–20% additional F-2 offspring will have to be raised and scored to provide power equivalent to that obtained in a cross between inbred lines. When a resource population contains novel favorable alleles at quantitative trait loci that are not present (or rare) in a commercial population, analyses of this sort will enable the loci of interest to be identified, mapped and manipulated effectively in breeding programs.Contribution no. 2124-E, 1987 series from The Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel 相似文献