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991.
1. The naturally occurring mitochondrial ATPase inhibitor inhibits the mitochondrial ATPase (F1) non-competitively.2. The interaction between inhibitor and inhibitor-depleted F1 or submitochondrial particles is diminished when the ratio of ATP/ADP is low or when energy is generated by substrate oxidation.3. The dissociation of the inhibitor from coupled Mg-ATP particles is promoted when substrates are being oxidized. This results in the appearance of a large uncoupler-stimulated ATPase activity. Activation of the uncoupler-stimulated ATPase activity is also achieved by incubation of the particles with ADP.4. The ATPase activity of Mg-ATP particles is determined by the turnover capacity of F1. When endogenous inhibitor is removed, energy dissipation becomes the rate-limiting step. This energy dissipation can be activated by an uncoupler.5. Evidence is presented for the existence of a non-inhibited intermediate F1-inhibitor complex. 相似文献
992.
F.-Nora Vögtle Björn Brändl Austin Larson Manuela Pendziwiat Marisa W. Friederich Susan M. White Alice Basinger Cansu Kücükköse Hiltrud Muhle Johanna A. Jähn Oliver Keminer Katherine L. Helbig Carolyn F. Delto Lisa Myketin Dirk Mossmann Nils Burger Noriko Miyake Audrey Burnett Ingo Helbig 《American journal of human genetics》2018,102(4):557-573
993.
Thatjana Gardeitchik Miski Mohamed Benedetta Ruzzenente Daniela Karall Sergio Guerrero-Castillo Daisy Dalloyaux Mariël van den Brand Sanne van Kraaij Ellyze van Asbeck Zahra Assouline Marlene Rio Pascale de Lonlay Sabine Scholl-Buergi David F.G.J. Wolthuis Alexander Hoischen Richard J. Rodenburg Wolfgang Sperl Zsolt Urban Eva Morava 《American journal of human genetics》2018,102(4):685-695
994.
Rosaria Saletti Simona Reina Maria G.G. Pittalà Andrea Magrì Vincenzo Cunsolo Salvatore Foti Vito De Pinto 《BBA》2018,1859(9):806-816
VDACs three isoforms (VDAC1, VDAC2, VDAC3) are integral proteins of the outer mitochondrial membrane whose primary function is to permit the communication and exchange of molecules related to the mitochondrial functions. We have recently reported about the peculiar over-oxidation of VDAC3 cysteines. In this work we have extended our analysis, performed by tryptic and chymotryptic proteolysis and UHPLC/High Resolution ESI-MS/MS, to the other two isoforms VDAC1 and VDAC2 from rat liver mitochondria, and we have been able to find also in these proteins over-oxidation of cysteines. Further PTM of cysteines as succination has been found, while the presence of selenocysteine was not detected. Unfortunately, a short sequence stretch containing one genetically encoded cysteine was not covered both in VDAC2 and in VDAC3, raising the suspect that more, unknown modifications of these proteins exist. Interestingly, cysteine over-oxidation appears to be an exclusive feature of VDACs, since it is not present in other transmembrane mitochondrial proteins eluted by hydroxyapatite. The assignment of a functional role to these modifications of VDACs will be a further step towards the full understanding of the roles of these proteins in the cell. 相似文献
995.
Monika Oláhová Wan Hee Yoon Kyle Thompson Sharayu Jangam Liliana Fernandez Jean M. Davidson Jennifer E. Kyle Megan E. Grove Dianna G. Fisk Jennefer N. Kohler Matthew Holmes Annika M. Dries Yong Huang Chunli Zhao Kévin Contrepois Zachary Zappala Laure Frésard Daryl Waggott Matthew T. Wheeler 《American journal of human genetics》2018,102(3):494-504
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998.
Imprints of multiple glacial refugia in the Pyrenees revealed by phylogeography and palaeodistribution modelling of an endemic spider
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Leticia Bidegaray‐Batista Alejandro Sánchez‐gracia Giulia Santulli Luigi Maiorano Antoine Guisan Alfried P. Vogler Miquel A. Arnedo 《Molecular ecology》2016,25(9):2046-2064
Mediterranean mountain ranges harbour highly endemic biota in islandlike habitats. Their topographic diversity offered the opportunity for mountain species to persist in refugial areas during episodes of major climatic change. We investigate the role of Quaternary climatic oscillations in shaping the demographic history and distribution ranges in the spider Harpactocrates ravastellus, endemic to the Pyrenees. Gene trees and multispecies coalescent analyses on mitochondrial and nuclear DNA sequences unveiled two distinct lineages with a hybrid zone around the northwestern area of the Catalan Pyrenees. The lineages were further supported by morphological differences. Climatic niche‐based species distribution models (SDMs) identified two lowland refugia at the western and eastern extremes of the mountain range, which would suggest secondary contact following postglacial expansion of populations from both refugia. Neutrality test and approximate Bayesian computation (ABC) analyses indicated that several local populations underwent severe bottlenecks followed by population expansions, which in combination with the deep population differentiation provided evidence for population survival during glacial periods in microrefugia across the mountain range, in addition to the main Atlantic and Mediterranean (western and eastern) refugia. This study sheds light on the complexities of Quaternary climatic oscillations in building up genetic diversity and local endemicity in the southern Europe mountain ranges. 相似文献
999.
Strong and stable geographic differentiation of swamp buffalo maternal and paternal lineages indicates domestication in the China/Indochina border region 总被引:2,自引:0,他引:2
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Yi Zhang Yongfang Lu Marnoch Yindee Kuan‐Yi Li Hsiao‐Yun Kuo Yu‐Ten Ju Shaohui Ye Md Omar Faruque Qiang Li Yachun Wang Vu Chi Cuong Lan Doan Pham Bounthong Bouahom Bingzhuang Yang Xianwei Liang Zhihua Cai Dianne Vankan Wallaya Manatchaiworakul Nonglid Kowlim Somphot Duangchantrasiri Worawidh Wajjwalku Ben Colenbrander Yuan Zhang Peter Beerli Johannes A. Lenstra J. Stuart F. Barker 《Molecular ecology》2016,25(7):1530-1550
The swamp type of the Asian water buffalo is assumed to have been domesticated by about 4000 years BP, following the introduction of rice cultivation. Previous localizations of the domestication site were based on mitochondrial DNA (mtDNA) variation within China, accounting only for the maternal lineage. We carried out a comprehensive sampling of China, Taiwan, Vietnam, Laos, Thailand, Nepal and Bangladesh and sequenced the mtDNA Cytochrome b gene and control region and the Y‐chromosomal ZFY, SRY and DBY sequences. Swamp buffalo has a higher diversity of both maternal and paternal lineages than river buffalo, with also a remarkable contrast between a weak phylogeographic structure of river buffalo and a strong geographic differentiation of swamp buffalo. The highest diversity of the swamp buffalo maternal lineages was found in south China and north Indochina on both banks of the Mekong River, while the highest diversity in paternal lineages was in the China/Indochina border region. We propose that domestication in this region was later followed by introgressive capture of wild cows west of the Mekong. Migration to the north followed the Yangtze valley as well as a more eastern route, but also involved translocations of both cows and bulls over large distances with a minor influence of river buffaloes in recent decades. Bayesian analyses of various migration models also supported domestication in the China/Indochina border region. Coalescence analysis yielded consistent estimates for the expansion of the major swamp buffalo haplogroups with a credibility interval of 900 to 3900 years BP. The spatial differentiation of mtDNA and Y‐chromosomal haplotype distributions indicates a lack of gene flow between established populations that is unprecedented in livestock. 相似文献
1000.
心血管疾病的流行是一个全球性的现象,在我国,其患病率也不断增长。目前尚无有效的治疗方法以解决心肌细胞损失这一关键问题,而干细胞移植很可能成为新的治疗方法。C-kit+心脏干细胞(cardiac stem/progenitor cell,CSCs)的发现证实了CSCs的存在,并为心脏的再生和修复治疗带来了新的曙光。C-kit+CSCs在心肌梗死、心力衰竭等心脏疾病中的作用得到了多项体内外实验及临床试验的证实,但由于目前尚缺乏直接的充分的证据证明内源性或外源性的c-kit+CSCs可分化为相当数量的成熟的具有功能的心肌细胞,其治疗机制尚存争议,同时,将其应用于临床仍面临多个问题。 相似文献