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排序方式: 共有349条查询结果,搜索用时 15 毫秒
61.
Here we present the first isolation of major histocompatibility complex (MHC) class I genes from two ancient fish, paddlefish (Polyodon spathula) and Chinese sturgeon (Acipenser sinensis). Seventeen sequences obtained showed high polymorphism and positive natural selection with dN/dS > 1. Evolutionary relationships revealed that sequences from paddlefish and Chinese sturgeon distinguished from other vertebrate class I and had an intermingling of alleles, which indicates that Acipenseriformes have a common ancestral gene of class I and a trans-species polymorphism across Acipenseriformes. We also found clear evidence of recombination among class I genes of paddlefish and Chinese sturgeon. 相似文献
62.
John Edwards 《FEBS letters》2010,584(5):1047-1053
Using conservation of synteny I show how the four thymosins expressed by teleost fish are related to the three of tetrapods, which is not evident from their protein sequences. This clarification was aided by identification of a novel thymosin of reptilians that replaces the β10 thymosin of mammals. Recent reconstruction of the ancestral vertebrate genome suggests that divergence of β-thymosins began with duplication preceding the two rounds of whole genome duplication. 相似文献
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64.
Interannual variation in experimental field conditions produce variability in the results of experiments monitored over multiple years, termed here “year effects.” When experimental treatments are replicated in separate years, interannual variation may influence treatment effects and produce significant treatment by initiation‐year interactions. Understanding the frequency and strength of these effects requires initiating identical experiments across years. We conducted a review of literature covering more than 500 experimental articles published in 7 journals between 1966 and 2008. Only 5% of the 276 general ecological field studies initiated experiments in multiple years. This rarity was even more evident in the journal Restoration Ecology, in which none of the 173 surveyed experimental studies initiated experiments in multiple years. In contrast, 48% of the 58 field experiments published in an agronomy journal were replicated across years. We found only 17 studies that tested treatment by initiation‐year interactions. Despite their rarity, 76% of these studies found significant interactions between treatment and initiation year. We conclude that the results of many ecological field experiments are likely to be contingent on the year in which they are implemented. We discuss the importance of treatment by initiation‐year interactions in ecology and restoration, factors that have hindered the inclusion of temporal replication in the past, and some suggestions for the appropriate design and analysis of temporally replicated experiments. We argue for more deliberate investigation of temporal contingency in ecological experimentation, especially in the field of restoration ecology, which may be particularly sensitive to treatment by initiation‐year interactions. 相似文献
65.
巴音布鲁克高寒人工草地土壤可培养微生物区系特征 总被引:1,自引:0,他引:1
以巴音布鲁克建植期为4、8、15和20年的4种人工草地为对象,以天然草地为对照,采用平板表面涂抹法对0~20cm、20~40cm的土壤微生物区系特征进行了分析。结果表明:细菌数量以建植期4年的草地土壤最多,20年最少,8年、15年人工草地分别是天然草地的1.19和0.58倍;真菌数量为4年人工草地>20年人工草地>15年人工草地>8年人工草地>天然草地;放线菌数量以天然草地最多,8年人工草地次之,20年、4年、15年人工草地依次降低;细菌、真菌以及放线菌数量均在8月达到最大值,9月最小;细菌与植被盖度呈显著正相关(P<0.05),微生物总数与植被盖度呈极显著正相关(P<0.01),真菌与土壤含水量呈极显著正相关(P<0.01),与电导率呈显著正相关(P<0.05),pH值对放线菌的影响最大;影响微生物类群总数的主要生态因子是植被盖度和建植年限。 相似文献
66.
Mary A. Napier Irving H. Goldberg Otto D. Hensens Ray S. Dewey Jerrold M. Liesch Georg Albers-Schönberg 《Biochemical and biophysical research communications》1981,100(4):1703-1712
On the basis of spectroscopic evidence, opening of a five-membered cyclic carbonate ring (1,3-dioxolan-2-one) in the C15-subunit of the previously determined partial structure (Fig. 1) of the major neocarzinostatin chromophore (NCS-Chrom ), is proposed to account for its base-catalyzed methanolysis to NCS-Chrom . NCS-Chrom , apparently an authentic natural product present as a minor component in all preparations of NCS studied, was found to be formally equivalent to the hydrolysis/decarboxylation product of the cyclic carbonate functionality in NCS-Chrom . The mercaptan-dependent DNA strand-scission activity, equivalent for NCS-Chrom , and , is independent of the integrity of the cyclic carbonate ring system and implicates a secondary site in the C15-substructure for mercaptan activation. 相似文献
67.
68.
Klaas Nicolay Klaas J. Hellingwerf Robert Kaptein Wil N. Konings 《Biochimica et Biophysica Acta (BBA)/Molecular Cell Research》1982,720(3):250-258
13C-nuclear magnetic resonance was used to study the metabolism of [2-13C]acetate in suspensions of Rhodopseudomonas sphaeroides. In the dark, in logarithmic-phase cells the 13C label appeared first in butyrate C-2 and C-4 and subsequently in glutamate C-4 and succinate C-2 and C-3. In the light, synthesis of poly(β-hydroxybutyrate) (PHB) takes place. Butyrate synthesis seems to be independent of PHB synthesis or degradation activity. Starved, logarithmic-phase cells also show massive synthesis of PHB in the dark. Stationary-phase cells incorporate 13C predominantly into glutamate and succinate. No significant butyrate biosynthesis can be detected in the dark or during illumination. The incorporation of label in PHB is very slow in these cells and most probably originates from exchange of 12C for 13C into PHB. This might indicate slow turnover without net synthesis of the polymer occurring under these conditions. The results are discussed in relation to the redox state and the availability of metabolic energy for biosynthetic reactions in the dark and during illumination of cell suspensions of Rps. sphaeroides. 相似文献
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70.
Dominique Jolly I. Colin Prentice Raymonde Bonnefille Aziz Ballouche Martin Bengo Patrice Brenac Guillaume Buchet David Burney Jean-Pierre Cazet Rachid Cheddadi Therese Edorh Hilaire Elenga Saida Elmoutaki Joel Guiot Fouzia Laarif Henry Lamb Anne-Marie Lezine Jean Maley Muaka Mbenza Odile Peyron Maurice Reille Isabelle Reynaud-Farrera Guy Riollet Jim C. Ritchie Emile Roche Louis Scott Immaculate Ssemmanda Herbert Straka Mohammed Umer Elise Van Campo Sikiminywa Vilimumbalo Annie Vincens Martyn Waller 《Journal of Biogeography》1998,25(6):1007-1027
Biome reconstruction from pollen and plant macrofossil data provides an objective method to reconstruct past vegetation. Biomes for Africa and the Arabian peninsula have been mapped for 6000 years bp and provide a new standard for the evaluation of simulated palaeovegetation distributions. A test using modern pollen data shows the robustness of the biomization method, which is able to predict the major vegetation types with a high confidence level. The application of the procedure to the 6000 years data set (pollen and plant macrofossil analyses) shows systematic differences from the present that are consistent with the numerous previous regional and continental interpretations, while providing a more extensive and more objective basis for such interpretations. Madagascar, eastern, southern and central Africa show only minor changes in terms of biomes, compared to present. Major changes in biome distributions occur north of 15°N, with steppe in many low-elevation sites that are now desert, and temperate xerophytic woods/scrub and warm mixed forest in the Saharan mountains. These shifts in biome distributions imply significant changes in climate, especially precipitation, between 6000 years and present, reflecting a change in monsoon extent combined with a southward expansion of Mediterranean influence. 相似文献