排序方式: 共有43条查询结果,搜索用时 31 毫秒
41.
Allan P. Jarvis Jr. Theresa A. Grdina Maureen F. Sullivan 《In vitro cellular & developmental biology. Plant》1986,22(10):589-596
Summary A new microencapsulation technology, developed for the encapsulation of living cells, has been demonstrated to be useful for
the study of growth and differential gene expression using Friend erythroleukemic cells cultured at high cell densities. Using
this technology, cultures of FL Clone 745 cells were encapsulated within semipermeable membranes composed of cross-linked
alginic acid and poly-l-lysine. Cell growth studies measuring total cell number demonstrated an average generation time of 8.5 h in 5% (vol/vol)
microcapsule cultures vs. 8.0 h in suspension cultures. Similar microcapsule cultures were serially propagated for more than
90 cell generations (13 sequential passages) with no significant change in this growth rate. In addition, final culture densities
of greater than 1.0×108 cells/ml of intracapsular volume were attained using a 3% (vol/vol) microcapsule culture in conjunction with a standard refeeding
schedule. Comparison of the level of dimethyl sulfoxide-induced hemoglobin production in suspension and microcapsule cultures
demonstrated that the total amount of hemoglobin produced on a per cell basis was comparable in both systems. Due to the retention
characteristics of the semipermeable membrane, the concentration of detergent-released hemoglobin, relative to other released
protein, was approximately twofold higher in microcapsule cultures than in control suspension cultures. 相似文献
42.
43.
Yoshiko Arimitsu Masaaki Seki Minoru Nakao Kenji Miyamoto 《Microbiology and immunology》1994,38(4):269-272
Results of a passive microcapsule agglutination test using 2 Japanese strains, Borrelia garinii and Borrelia afzelii, were comparable to that of the passive microcapsule agglutination test using a Swiss strain Borrelia burgdorferi IRS in detecting antibodies of an early stage of Lyme disease patients in Japan. MCAT with microcapsule sensitized strain IRS seems likely to become one of the important tools for the early diagnosis of Lyme disease in the world. The highest titer of MCAT was detected in the serum samples of patients which were taken around 2 weeks after erythema chronicum migrans. The test is very simple and can be employed easily in diagnostic laboratories. 相似文献