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11.
R. R. Colwell 《Biodiversity and Conservation》1992,1(4):342-345
Concluding remarks from the joint IUBS/IUMS workshop on Biodiversity amongst microorganisms and its relevance held in Amsterdam on 7–8 September 1991. An international microbial ecology programme can be justified in its own right now that appropriate investigative tools have been developed. Microorganisms influence global change, and indicate global health and environmental quality. At the same time, an inventory of the world's microbial species and their properties is required, together with associated culture collections and genomes. Sampling methods need to be standardized, both for species and functions. Extreme environments are a particularly rich source of microbial genomes, and endangered habitats should be sampled as a matter of priority. Cataloguing and conserving the world's microbial biodiversity is justifiable and scientifically important. 相似文献
12.
Phagotrophic phytoflagellates in microbial food webs 总被引:7,自引:2,他引:5
Karen Glaus Porter 《Hydrobiologia》1988,159(1):89-97
Phagotrophy by pigmented flagellates is known from the literature but has recently been rediscovered in the context of microbial
food webs. Particle ingestion rates were found to be equivalent for pigmented and nonpigmented microflagellates in both field
and laboratory studies. Ingestion rates of the chrysophytes Ochromonas danica, O. minuta, and Poterioochromonas malhamensis, the dinoflagellate Peridinium inconspicuum, and the cryptophytes Cryptomonas ovata and C. erosa were compared with those of two nonpigmented Monas species using 0.57 μm polystyrene beads as a food source. Ingestion rates were 0.31 to 3.17 beads/cell/h and filtration rates
were 10−7 to 10−8 ml/cell/h with no detectable difference between pigmented and nonpigmented forms. Ingestion rates in unpigmented Monas species showed a linear increase with increasing particle concentration from 1.9 × 106 to 1.6 × 107 beads/ml.
Light and DOC levels in the range of those encountered by phytoflagellates in the field also influenced laboratory measurements
of bead ingestion by Poterioochromonas malhamensis. Ingestion rates decreased and photosynthesis increased over the natural PAR light range from 0 to 1800 microeinsteins/s/m2. At 40 microeinsteins/s/m2 maximum ingestion rates and high rates of photosynthesis occurred simultaneously. Ingestion rates decreased above 4 mgC/l
supplied as glucose. DOC levels commonly occurring in Lake Oglethorpe range from 3.5 to 10.0 mgC/l. These studies suggest
that mixotrophy, the trophic utilization of particulate food and dissolved organic matter as well as photosynthetically fixed
organic matter, is a balanced process that can be regulated by environmental conditions.
In field studies during a chrysophyte bloom, phytoflagellate grazing exceeded heterotrophic microflagellate grazing and constituted
up to 55% of the bactivory of all microflagellates, ciliates, rotifers, and crustaceans combined. Neither bacterial abundance,
light nor temperature were good predicters of grazing rates for the phagotrophic phytoflagellate association as a whole during
this unstratified period. Phagotrophs are often most abundant at the metalimnetic plate during stratification. 相似文献
13.
Spatial patterns and feeding of meiobenthic harpacticoid copepods in relation to resident microbial flora 总被引:2,自引:2,他引:0
Distributional and feeding relationships of harpacticoid copepods and their microbial prey were examined in a tidal channel at Great Sippewissett Marsh. A horizontal zonation of photosynthetic microorganisms was composed of: 1) a diatom area; 2) a purple sulfur bacterial (Thiocapsa sp.) area; and 3) a clear area. Four species of harpacticoid copepods were associated with given areas. Leptocaris brevicornis occurred in very high densities in the diatom area but in relatively low densities in other areas. Mesochra lilljeborgi occurred in significantly higher densities in the purple and clear areas.Feeding experiments, using resident microbial flora labelled with NaH14CO3 and 3H-thymidine, were conducted to determine which foods are 1) ingested but simply pass through the gut and 2) ingested, and retained. These experiments indicated that L. brevicornis ingested diatoms and the heterotrophs associated with the diatoms, but only retained the heterotrophic portion. Microscopic examination indicated that diatoms were passed out intact in feces. Oscillatoria sp. (cyanobacterium) was not ingested. Mesochra lilljeborgi ingested Spirulina sp. (cyanobacterium), Thiocapsa sp., and the heterotrophs associated with Thiocapsa but only retained the Thiocapsa label.These data for harpacticoids suggest that spatial distributions of meiofauna may be closely coupled with microbial food organisms which they consume. Also, that while several microbial foods may be ingested, only certain microbes are digested and assimilated as a food resource, further indicating the complexity of feeding relationships among the meiofauna. 相似文献
14.
Abstract Microbial transformation of N , N -dimethyl- p -phenylene diamine (DMPDA), a microbial product formed from the fungicide fenaminosulf ( p -dimethylaminobenzenediazo sodium sulfonate) was studied by enriching microbes in soils treated with the amine. Microorganisms isolated from DMPDA-treated soil belonged to the genera of Micrococcus, Alcaligenes , and Corynebacterium . Of the various isolates, Alcaligenes DM4 showed maximal growth on DMPDA utilizing it as sources of carbon and nitrogen. When grown in mineral salts basal medium containing 0.05% DMPDA to serve as carbon and nitrogen sources, Alcaligenes DM4 grew exponentially up to 18 h. Even though the characterization of the complete pathway of microbial degradation of DMPDA could not be carried out due to the auto-oxidation of the compound, the initial transformation product of DMPDA by Alcaligenes DM4 has been identified as a dimer. The dimer is generated into the culture medium presumably by the extra-cellular oxidase of Alcaligenes DM4 . It is suggested that the risk-benefit evaluation on the use of fenaminosulf is to be made taking into consideration the microbial transformations of the fungicide. 相似文献
15.
E. C. Jorgenson 《Journal of nematology》1984,16(2):154-158
Granular and liquid commercial humates, with micronutrients, and a microbial fermentation product were compared in several combinations with nematicides for their effects on cotton lint yield and root-knot nematode suppression. Fumigant nematicides effectively reduced cotton root galling caused by root-knot nematodes, and cotton lint yields increased. Organophosphates and carbamates were not effective. Occasionally, cotton lint yields were increased or maintained with combination treatments o f humates, micronutrients, and a microbial fermentation product, but galling o f cotton roots by root-knot nematodes was usually not reduced by these treatments. 相似文献
16.
Abstract From chemostat enrichments conducted at dilution rates of 0.025, 0.12 and 0.25 h−1 glutamate- and aspartate-fermenting bacteria were isolated. The dominant aspartate-fermenting strains in all these enrichments belonged to the genus Campylobacter , whereas 3 dissimilar types of glutamate-fermenting bacteria predominated at the different dilution rates. One of these strains was identified as Clostridium cochlearium . The remaining two were designated as strain DKglu16 (glutamate → acetate + propionate + ammonium + carbon dioxide) and DKglu21 (glutamate → acetate + formate + ammonium + carbon dioxide). Grown in continuous culture under glutamate limitation, strain DKglu16 (μmax = 0.13 h−1 ; K s = 1.9 μM) outcompeted C. cochlearium (μmax = 0.36 h−1 ; K s = 7 μM) at low dilution rates, but was outgrown at higher rates of dilution (0.044 h−1 ). In glutamate-limited continuous culture the competitiveness of strain DKglu16 increased considerably when lactate was added to the feed in addition to glutamate. 相似文献
17.
Proteins and peptides in water-restricted environments 总被引:5,自引:0,他引:5
M Waks 《Proteins》1986,1(1):4-15
18.
Specific protein inhibitors of microbial serine proteinases were isolated from wheat (Triticum aestivum L.), rye (Secale cereale L.) and triticale using affinity chromatography on subtilisin-Sepharose 4B. The wheat inhibitor had an isoelectric point (pI) at pH 7.2, while the rye inhibitor consisted of two forms with pI values of 6.8 and 7.1. In triticale, two components were present with pIs 7.2 and 6.8. All the inhibitors had M
r values of approx. 20 000. The isolated proteins were effective inhibitors of subtilisins Carlsberg and BPN, and of fungal proteinases (EC 3.4.21.14) from the genus Aspergillus, but they were completely inactive against trypsin (EC 3.4.21.4) chymotrypsin (EC 3.4.21.1) and pancreatic elastase (EC 3.4.21.36). The inhibitors formed complexes with subtilisin in a molar ratio of 1:1. The results of chemical modifications seem to indicate that the isolated inhibitors have methionine residues in their reactive sites.Abbreviation pI
isoelectric point 相似文献
19.
Three post-emergence herbicides (2,4-D, picloram and glyphosate) were applied to samples of an Alberta agricultural soil at
concentrations of 0, 2, 20, and 200 μg g−1. The effects of these chemicals on certain microbial variables was monitored over 27 days. All herbicides caused enhancement
of basal respiration but only for 9 days following application, and only for concentrations of 200 μg g−1. Substrate-induced respiration was temporarily depressed by 200 μg g−1 picloram and 2,4-D, and briefly enhanced by 200 μg g−1 glyphosate. It is concluded that because changes in microbial variables only occurred at herbicide concentrations of much
higher than that which occurs following field application, the side-effects of these chemicals is probably of little ecological
significance. 相似文献
20.
Comparative study of two meromictic basins of Lake Banyoles (Spain) with sulphur phototrophic bacteria 总被引:1,自引:1,他引:0
The annual limnological dynamics of two meromictic basins of Lake Banyoles (C-III and C-IV) have been studied and compared
on the basis of their physical, chemical and biological characters. Stability values calculated for both basins gave 865 g
cm cm−2 and 495 g cm cm−2 for C-III and C-IV respectively. These values are in agreement with the fact that C-IV was almost completely mixed during
winter. In this basin, during stratification, the monimolimnion increased in thickness as the stability increased. Isolation
of the respective monimolimnia resulted in the development of anoxic conditions and the accumulation of sulphide in both C-III
and C-IV, which favoured the development of dense populations of sulfur phototrophic bacteria. The purple sulphur bacterium
Chromatium minus and the green sulphur bacterium Chlorobium phaeobacteroides were identified as the main components of these photosynthetic populations. The different depths at which the O2/H2S boundary was situated in both basins (and consequently the different light intensity reaching this zone) determined the
growth of these bacteria. Light intensities at the chemocline of C-IV reached values up to 5% of surface incident light. In
contrast, in C-III this variable was sensibly lower, with values depending on season and seldom reaching 1%. Phototrophic
bacteria were consequently found earlier in C-IV than in C-III, where no significant concentrations were found until August.
Finally stability is discussed as an important factor controlling chemical and biological dynamics in meromictic lakes. 相似文献