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51.
The interactions between the mycorrhizal fungusGlomus mosseae, the plant pathogenPythium ultimum, and a pathogen-antagonistTrichoderma aureoviride in the rhizosphere ofTagetes erecta (marigold) were studied for their effects on plant growth in a peat-perlite substrate. Mycorrhizal fungus inoculation protected the plant againstP. ultimum, since both phytomass production and foliar development were higher in mycorrhizal plants.T. aureoviride had no effect on nonmycorrhizal plants in the presence or absence ofP. ultimum. However, more biomass was produced by mycorrhizal plants whenT. aureoviride was present, whether or not soil was infested withP. ultimum. ei]R Rodriguez-Kabana 相似文献
52.
Fourier transform infrared spectroscopic study of ion binding and intramolecular interactions in the polar head of digalactosyldiacylglycerol 总被引:2,自引:0,他引:2
Lipid bilayers composed of digalactosyldiacyl-glycerol (DGDG), that is, Galp1-6Galp1-3DAG, a non-ionic lipid of the thylakoid membrane of chloroplasts, aggregate in aqueous media containing mono- and divalent cations in amounts above a threshold concentration (Ct) of about 1.0, 4.7 and 10.0 mM for Ca2+, Mg2+ and Na+, respectively. In this work, we found that above Ct the DGDG membranes do not undergo fusion and that the aggregation can be reversed, or disrupted. This means that the perturbation induced by the salts results from adsorption, or complexation of the ions in the polar head of DGDG. To investigate this question, we used Fourier transform infrared (FTIR) spectroscopy to identify the molecular sites in DGDG which are modified by interaction, or adduct formation with CaCl2, MgCl2 and NaCl. We also determined whether the ions affect the intramolecular hydrogen bonding between the sn2 ester C = O and the carbon-6 of the -anomer of galactose (Gal). The major conclusions are: (i) the salts do not affect, at least directly, the, ester carbonyl region of DGDG, (ii) the most probable sites of binding, or adsorption, for the ions are the ring oxygen, and (iii) the ring hydroxyls are the sites of either ion complexation or intra- and intermolecular H-bonding in interacting DGDG membranes. Within this framework, the complexation of the ions with Gal might induce total or partial dehydration of the galactolipid headgroup and thus provides the means to overcome the repulsive hydration forces that hinder aggregation of the DGDG membranes.Abbreviations DGDG
digalactosyldiacylglycerol
- EDTA
ethylenediaminetetracetic acid
- FTIR
Fourier transform infrared
- Gal
galactose
- GIDG
D-glucosyldiacylglycerol
- Glyc
glycerol
- LHCII
chloroplast light harvesting complex II
- MGDG
monogalactosyldiacylglycerol
- PC
phosphatidylcholine
- PG
phosphatidylglycerol
- PS
phosphatidylserine
- SQDG
sulfoquinovosyl-diacylglycerol
Correspondence to: M. Fragata 相似文献
53.
La Salada de Chiprana Lake, located in the Ebro River basin, northeastern Spain, is the only permanent and deep water hypersaline ecosystem in all of western Europe. With a total surface of 31 ha and a maximum depth of 5.6 m, it has several basins bounded by elongated sandstone-bodies or ribbons which are paleochannels of Miocene age. Its salinity varied from 30 to 73 g 1–1 during the 1989 hydrological cycle and the most abundant ions were magnesium and sulphate. Depth-time distributions of major physico-chemical variables demonstrated that the lake was stratified in two distinctive layers during most of the year. The chemocline disappeared only in October, with the complete overturn of the water column. In the deep water, three conditions occurred which allowed development of green sulphur bacteria populations: (1) oxygen depletion, (2) presence of hydrogen sulphide and (3) presence of light. Benthic microbial mats covered the sediments of shallow shores of moderate slope. 相似文献
54.
The possibility of continuously measuring the heat produced by microorganisms in an ordinary laboratory fermentor was studies. An inventory of the heat flows influencing the temperature of the culture was made. The magnitude and standard deviation in these heat flows were studied from theoretical and practical viewpoints. Calibration procedures were tested, and a model describing the heat flows in steady state and during dynamic conditions was made. Microbial heat production could be calculated accurately with the help of this model, appropriate temperature measurements, and equipment properties measured during the calibration procedures. It was found that the measurement of heat production could be done with an accuracy similar to that in the O(2) uptake measurement. (c) 1993 John Wiley & Sons, Inc. 相似文献
55.
Kinetics of competitive inhibition and cometabolism in the biodegradation of benzene, toluene, and p-xylene by two Pseudomonas isolates 总被引:1,自引:0,他引:1
Two Pseudomonas species (designated strains B1 and X1) were isolated from an aerobic pilot-scale fluidized bed reactor treating groundwater containing benzene, toluene, and p-xylene (BTX). Strain B1 grew with benzene and toluene as the sole sources of carbon and energy, and it cometabolized p-xylene in the presence of toluene. Strain X1 grew on toluene and p-xylene, but not benzene. In single substrate experiments, the appearance of biomass lagged the consumption of growth substrates, suggesting that substrate uptake may not be growth-rate limiting for these substrates. Batch tests using paired substrates (BT, TX, or BX) revealed competitive inhibition and cometabolic degradation patterns. Competitive inhibition was modeled by adding a competitive inhibition term to the Monod expression. Cometabolic transformation of nongrowth substrate (p-xylene) by strain B1 was quantified by coupling xylene transformation to consumption of growth substrate (toluene) during growth and to loss of biomass during the decay phase. Coupling was achieved by defining two transformation capacity terms for the cometabolizing culture: one that relates consumption of growth substrate to the consumption of nongrowth substrate, and second that relates consumption of biomass to the consumption of nongrowth substrate. Cometabolism increased decay rates, and the observed yield for strain B1 decreased in the presence of p-xylene. (c) 1993 Wiley & Sons, Inc. 相似文献
56.
The spring dispersal ofSitona lineatus L. (Coleoptera; Curculionidae) was investigated on a Danish farm.S. lineatus dispersed by flight in the early spring on sunny, calm days with temperatures above ca. 15°C.
Two thirds of the population ofS. lineatus dispersed from perennial leguminous crops (clover and lucerne) in the first period of flight activity. The next dispersal
did not occur until one month later despite several intermediate flight activity periods. The first period of dispersal occurred
before the germination of the spring sown summer host crop,Vicia faba L. The field bean crop was infested in three later invasions during a period of more than three weeks.
The aggregation pheromone, 4-methyl-3,5-heptanedione, had a significant effect on captures of both males and females in cone
traps placed on the ground. There was no effect of the pheromone on captures in yellow sticky traps placed 1.5 m above ground.
The pheromone effect is discussed in relation to behavioural observations.
Both types of traps may be used in a survey system for monitoring spring dispersal ofS. lineatus and optimal timing of insecticide spraying. However, the pheromone cone traps were highly specific whereas all kinds of flying
insects were caught in the yellow sticky traps, thus making the latter traps less suitable for monitoring. 相似文献
57.
Robin W. Renaut 《Hydrobiologia》1993,267(1-3):75-98
Benthic microbial mats are common in the alkaline hydromagnesite-magnesite playa lakes of Interior British Columbia. Four main zones are recognized based on mat morphology that can be related to the type and duration of wetting. From the basin margin toward the playa centre they are: (i) vegetated hummocky ground; (ii) polygonal hummocky ground; (iii) low-domal and stratiform mats, and (iv) laterally continuous and pustular mats. Mats in peripheral mudflats are commonly mineralized by hydromagnesite, mostly precipitated by capillary evaporation of shallow groundwaters. Mats forming in the ephemeral lake tend to have lower carbonate content.Although widespread, the mats are poorly preserved in the Holocene sedimentary record. Underlying sediments are commonly weakly bedded, disturbed or massive. Desiccation, dehydration, wetting-drying cycles, and grazing by invertebrates cause fragmentation of mats at the surface, facilitating erosion. Cryogranulation, interstitial mineral precipitation, vesiculation, bioturbation, compaction, and volume changes associated with diagenesis, disrupt and destroy lamination in the upper few centimetres. Most surviving organic matter is lost by early microbial degradation. 相似文献
58.
Dianye Zhang Lu Wang Shuqi Qin Dan Kou Siyu Wang Zhihu Zheng Josep Peñuelas Yuanhe Yang 《Global Change Biology》2023,29(14):3910-3923
The status of plant and microbial nutrient limitation have profound impacts on ecosystem carbon cycle in permafrost areas, which store large amounts of carbon and experience pronounced climatic warming. Despite the long-term standing paradigm assumes that cold ecosystems primarily have nitrogen deficiency, large-scale empirical tests of microbial nutrient limitation are lacking. Here we assessed the potential microbial nutrient limitation across the Tibetan alpine permafrost region, using the combination of enzymatic and elemental stoichiometry, genes abundance and fertilization method. In contrast with the traditional view, the four independent approaches congruently detected widespread microbial nitrogen and phosphorus co-limitation in both the surface soil and deep permafrost deposits, with stronger limitation in the topsoil. Further analysis revealed that soil resources stoichiometry and microbial community composition were the two best predictors of the magnitude of microbial nutrient limitation. High ratio of available soil carbon to nutrient and low fungal/bacterial ratio corresponded to strong microbial nutrient limitation. These findings suggest that warming-induced enhancement in soil nutrient availability could stimulate microbial activity, and probably amplify soil carbon losses from permafrost areas. 相似文献
59.
频繁的人类生产活动使植被遭到破坏,造成基岩裸露的石漠化现象,严重制约了喀斯特地区自然和社会经济的发展,随着生态修复工程的大力开展,微生物群落的结构和功能在生态修复中逐渐受到重视,因为微生物作为喀斯特生态系统的重要组成部分,不仅在物质循环过程中起着重要作用,也在喀斯特生态系统修复中占有十分重要的地位。所以微生物群落结构和功能的研究可以作为衡量生态系统稳定性的重要指标。就中国喀斯特地区的典型植被恢复的不同阶段、成土过程、不同的土地利用方式、矿山修复过程以及不同水域中的微生物群落结构及功能等方面的研究状况进行系统梳理,结合实例综合论述喀斯特地区生态修复过程中微生物作用的研究进展,以期为喀斯特地区生态修复提供参考。 相似文献
60.
Protein liquid-liquid phase separation drives the dynamic assembly of membraneless organelles for fulfilling different physiological functions. Under diseased condition, protein may undergo liquid-to-solid condensation to form pathological amyloid aggregates closely associated with neurodegenerative diseases. Chemical probe serves as an important chemical tool not only for exploring the basic principle of the dynamic assembly of different protein condensates in vitro and in cell but also for clinical diagnosis and therapeutics of the related diseases. In this review, we first introduce chemical probes to image and regulate protein condensates. Then, we summarized three different categories of chemical probes including general amyloid dye, selective positron emission tomography tracer, and disaggregating binder, which feature distinct interaction pattern and activity upon binding to different pathological amyloid fibrillar aggregates. Next, we discuss the development of chemical probes for tracking protein amorphous aggregates in cells. Finally, we point out future direction in expanding the probes’ chemical space and applications. 相似文献