全文获取类型
收费全文 | 1265篇 |
免费 | 70篇 |
国内免费 | 28篇 |
专业分类
1363篇 |
出版年
2024年 | 4篇 |
2023年 | 13篇 |
2022年 | 5篇 |
2021年 | 7篇 |
2020年 | 20篇 |
2019年 | 24篇 |
2018年 | 19篇 |
2017年 | 34篇 |
2016年 | 31篇 |
2015年 | 30篇 |
2014年 | 28篇 |
2013年 | 45篇 |
2012年 | 18篇 |
2011年 | 23篇 |
2010年 | 25篇 |
2009年 | 50篇 |
2008年 | 59篇 |
2007年 | 78篇 |
2006年 | 61篇 |
2005年 | 62篇 |
2004年 | 39篇 |
2003年 | 31篇 |
2002年 | 49篇 |
2001年 | 64篇 |
2000年 | 52篇 |
1999年 | 18篇 |
1998年 | 25篇 |
1997年 | 22篇 |
1996年 | 35篇 |
1995年 | 25篇 |
1994年 | 37篇 |
1993年 | 31篇 |
1992年 | 34篇 |
1991年 | 30篇 |
1990年 | 41篇 |
1989年 | 20篇 |
1988年 | 35篇 |
1987年 | 9篇 |
1986年 | 28篇 |
1985年 | 17篇 |
1984年 | 17篇 |
1983年 | 11篇 |
1982年 | 18篇 |
1981年 | 9篇 |
1980年 | 14篇 |
1978年 | 6篇 |
1977年 | 4篇 |
1976年 | 6篇 |
排序方式: 共有1363条查询结果,搜索用时 0 毫秒
71.
Constitutive presence of antibiotic resistance genes within the bacterial community of a large subalpine lake 下载免费PDF全文
Andrea Di Cesare Ester M. Eckert Alessia Teruggi Diego Fontaneto Roberto Bertoni Cristiana Callieri Gianluca Corno 《Molecular ecology》2015,24(15):3888-3900
The fate of antibiotic resistance genes (ARGs) in environmental microbial communities is of primary concern as prodromal of a potential transfer to pathogenic bacteria. Although of diverse origin, the persistence of ARGs in aquatic environments is highly influenced by anthropic activities, allowing potential control actions in well‐studied environments. However, knowledge of abundance and space–time distribution of ARGs in ecosystems is still scarce. Using quantitative real‐time PCR, we investigated the presence and the abundance of twelve ARGs (against tetracyclines, β‐lactams, aminoglycosides, quinolones and sulphonamides) at different sampling sites, depths and seasons, in Lake Maggiore, a large subalpine lake, and in the area of its watershed. We then evaluated the correlation between each ARG and a number of ecological parameters in the water column in the deepest part of the lake. Our results suggest the constitutive presence of at least four ARGs within the bacterial community with a high proportion of bacteria potentially resistant to tetracyclines and sulphonamides. The presence of these ARGs was independent of the total bacterial density and temperature. The dynamics of tet(A) and sulII genes were, however, positively correlated with dissolved oxygen and negatively to chlorophyll a, suggesting that the resistant microbes inhabit specific niches. These observations indicate that the lake is a reservoir of antibiotic resistances, highlighting the need of a deeper understanding of the sources of ARGs and the factors allowing their persistence in waters. 相似文献
72.
Sedimentation in Boreal Lakes—The Role of Flocculation of Allochthonous Dissolved Organic Matter in the Water Column 总被引:1,自引:0,他引:1
We quantified sedimentation of organic carbon in 12 Swedish small boreal lakes (<0.48 km2), which ranged in dissolved organic carbon (DOC) from 4.4 to 21.4 mg C l−1. Stable isotope analysis suggests that most of the settling organic matter is of allochthonous origin. Annual sedimentation
of allochthonous matter per m2 lake area was correlated to DOC concentration in the water (R
2 = 0.41), and the relationship was improved when sedimentation data were normalized to water depth (R
2 = 0.58). The net efflux of C as CO2 from the water to the atmosphere was likewise correlated to DOC concentration (R
2 = 0.52). The losses of organic carbon from the water column via mineralization to CO2 and via sedimentation were approximately of equal importance throughout the year. Our results imply that DOC is a precursor
of the settling matter, resulting in an important pathway in the carbon cycle of boreal lakes. Thus, flocculation of DOC of
terrestrial origin and subsequent sedimentation could lead to carbon sequestration by burial in lake sediments. 相似文献
73.
长江流域若干水体寡毛类区系组成及相似性分析 总被引:5,自引:0,他引:5
研究了长江流域十余个水体水栖寡毛类的区系组成及分布。共鉴定水栖寡毛类53种,隶3科27属,其中仙女虫科15属31种(占58.49%);颤蚓科11属20种(占37.74%);线蚓科1属2种(占3.77%).在河流、草型湖泊和藻型湖泊这三类水体中,以草型湖泊的物种数最多,达36种;其次为长江干流,34种;藻型湖泊仅24种。对寡毛类的种间关系及不同生境寡毛类种类组成的相似性进行了聚类分析,结果表明,寡毛类的种类分布具有明显的地域性,据此可将其划分为6类(A-F组).
相似文献
74.
M.J. Siegert A. Behar M. Bentley D. Blake S. Bowden P. Christoffersen C. Cockell H. Corr D. C. Cullen H. Edwards A. Ellery C. Ellis-Evans G. Griffiths R. Hindmarsh D. A. Hodgson E. King H. Lamb L. Lane K. Makinson M. Mowlem J. Parnell D. A. Pearce J. Priscu A. Rivera M. A. Sephton M. R. Sims A . M. Smith M. Tranter J. L. Wadham G. Wilson J. Woodward 《Reviews in Environmental Science and Biotechnology》2007,6(1-3):161-179
Antarctic subglacial lakes have, over the past few years, been hypothesised to house unique forms of life and hold detailed
sedimentary records of past climate change. Testing this hypothesis requires in situ examinations. The direct measurement
of subglacial lakes has been considered ever since the largest and best-known lake, named Lake Vostok, was identified as having
a deep water-column. The Subglacial Antarctic Lake Environments (SALE) programme, set up by the Scientific Committee on Antarctic
Research (SCAR) to oversee subglacial lakes research, state that prior exploration of smaller lakes would be a “prudent way
forward”. Over 145 subglacial lakes are known to exist in Antarctica, but one lake in West Antarctica, officially named Ellsworth
Subglacial Lake (referred to hereafter as Lake Ellsworth), stands out as a candidate for early exploration. A consortium of
over 20 scientists from seven countries and 14 institutions has been assembled to plan the exploration of Lake Ellsworth.
An eight-year programme is envisaged: 3 years for a geophysical survey, 2 years for equipment development and testing, 1 year
for field planning and operation, and 2 years for sample analysis and data interpretation. The science experiment is simple
in concept but complex in execution. Lake Ellsworth will be accessed using hot water drilling. Once lake access is achieved,
a probe will be lowered down the borehole and into the lake. The probe will contain a series of instruments to measure biological,
chemical and physical characteristics of the lake water and sediments, and will utilise a tether to the ice surface through
which power, communication and data will be transmitted. The probe will pass through the water column to the lake floor. The
probe will then be pulled up and out of the lake, measuring its environment continually as this is done. Once at the ice surface,
any water samples collected will be taken from the probe for laboratory analysis (to take place over subsequent years). The
duration of the science mission, from deployment of the probe to its retrieval, is likely to take between 24 and 36 h. Measurements
to be taken by the probe will provide data about the following: depth, pressure, conductivity and temperature; pH levels;
biomolecules (using life marker chips); anions (using a chemical analyzer); visualisation of the environment (using cameras
and light sources); dissolved gases (using chromatography); and morphology of the lake floor and sediment structures (using
sonar). After the probe has been retrieved, a sediment corer may be dropped into the lake to recover material from the lake
floor. Finally, if time permits, a thermistor string may be left in the lake water to take time-dependent measurements of
the lake’s water column over subsequent years. Given that the comprehensive geophysical survey of the lake will take place
in two seasons during 2007–2009, a two-year instrument and logistic development phase from 2008 (after the lake’s bathymetry
has been assessed) makes it possible that the exploration of Lake Ellsworth could take place at the beginning of the next
decade. 相似文献
75.
Cladoceran populations, as revealed by sedimentary remains, were studied in six lakes which by diatom evidence have become
strongly acidified during the past 30 years. The accumulation rates of cladoceran remains and in two of the most strongly
acidified lakes both relative and absolute numbers ofBosmina greatly increased during this period. Species numbers were not reduced, but there were shifts in relative abundances of cladoceran
species.Bosmina longispina dominated all the lakes. Low pH (4.7) seemed not to be straight-forwardly harmful toDaphnia longispina, but the relative abundances of this taxon were reduced because ofBosmina. 相似文献
76.
J. F. Talling 《Hydrobiologia》1986,138(1):139-160
Although some study of the subject began in 1899, wide-ranging information from African water-bodies has only become available since 1950. Important developments included the establishment of long-term centres of research, the adoption of improved methods for quantitative algal sampling, the more intensive study of environmental conditions, the beginnings of experimental testing, and the improvement of taxonomic knowledge.At higher latitudes (> 20 °) examples of pronounced algal seasonality are long-established; they are accompanied and influenced by marked changes in radiant energy income and so water temperature, and often by effects of seasonal water input. Illustrations are given from lakes in Morocco and South Africa.More generally in Africa, including the tropical belt, annual patterns of phytoplankton seasonality are usually either dominated by hydrological features (water input-output) or by hydrographic ones (water-column structure and circulation). Examples of both types are discussed, together with instances (e.g. L. Volta) of combined hydrological and hydrographic regulation. In both the seasonal abundance of diatoms is often distinct and complementary to that of blue-green algae, with differing relationships to vertical mixing and water retention.Horizontal variability in the seasonal cycle is especially pronounced in the larger or morphometrically subdivided lakes. Some inshore-offshore differentiation is also known to affect phytoplankton quantity (e.g. L. George) and species composition (e.g. L. Victoria). Longitudinal differentiation is common in elongate basins especially when with a massive or seasonal inflow at one end (e.g. L. Turkana, L. Nubia, L. Volta); occasional terminal upwelling can also be influential (e.g. southern L. Tanganyika). Such examples grade into the longitudinally differentiated seasonality of flowing river-reservoir systems, as studied on the Blue and White Niles.The annual amplitude of population density, expressed in orders of magnitude (=log10 units), is one measure of seasonal variability. It can exceed 3 orders both in systems subject to hydrological wash-out (e.g. Nile reservoirs) and in the more variable species components of lakes of long retention (e.g. L. Victoria). Low amplitudes can be characteristic of some components (e.g. green algae in L. Victoria) or of total algal biomass (e.g. L. George, L. Sibaya).Seasonal changes may be subordinated to inter-annual ones, especially in shallow and hydrologically unstable lakes (e.g. L. Nakuru). 相似文献
77.
C. Granado-Lorencio J. Lobón Cerviá C. R. M. Araujo Lima 《Biodiversity and Conservation》2007,16(3):679-692
The Neotropical region is renowned for its high biodiversity, and the Amazon River basin contains the highest number of fish
species of any river system in the world. In recent years, habitat fragmentation and exploitation of biotic resources have
threatened biological integrity and provoked to need for sustainable management and conservation of the Amazon River system.
We studied 36 floodplain lakes along 2000 km of the Amazon River. The fish assemblages associated with flood forests are moderately
diverse, with low species dominance and reduced populations. To detect nestedness of fish assemblage composition in floodplain
lakes, a nested subset analysis was performed on species presence–absence. The incidence matrix (species × lakes) was maximally
packed using the Nestedness Temperature Calculator software. The results of ranking lakes and species allow us to establish
targets for conservation. Such strategy for sustainable management should be focused on maintaining the Amazonian biodiversity. 相似文献
78.
Total concentrations of algal pigments, organic C, C, N, P and S were determined in surface sediments from the littoral zone
of 21 lakes in ice-free areas of northern Victoria Land (Antarctica) with different climatic and environmental conditions.
Concentrations of major ions and nutrients were also determined in water samples from the same lakes. The latter samples had
extremely variable chemical compositions; however, all the lakes resulted oligotrophic. Pigment concentrations in surface
sediments were comparable to those reported for other Antarctic lakes and lower than those in oligotrophic lakes at lower
latitudes. Cyanophyta, Chlorophyta and Bacillariophyta were the main taxa identified. These taxa correspond to those reported
in previous microscopy-based studies on Antarctic phytoplankton and phytobenthos. Discriminant Function Analysis and Canonical
Correspondence Analysis of data indicate that the distribution of pigments in these Victoria Land lakes depends mainly on
their geographical location (particularly the distance from the sea) and nutrient status. 相似文献
79.
Phylogenetic analysis of freshwater sponges provide evidence for endemism and radiation in ancient lakes 总被引:2,自引:0,他引:2
Meixner MJ Lüter C Eckert C Itskovich V Janussen D von Rintelen T Bohne AV Meixner JM Hess WR 《Molecular phylogenetics and evolution》2007,45(3):875-886
Morphologic and phylogenetic analysis of freshwater sponges endemic to lakes in Central Sulawesi, Siberia and South-East Europe is presented. We also analyzed several cosmopolitan sponge species from Eurasia and North America and included sponge sequences from public databases. In agreement with previous reports [Addis, J.S., Peterson, K.J., 2005. Phylogenetic relationships of freshwater sponges (Porifera, Spongillina) inferred from analyses of 18S rDNA, COI mtDNA, and ITS2 rDNA sequences. Zool. Scr. 34, 549-557], the metaniid sponge Corvomeyenia sp. was the most deeply branching species within a monophyletic lineage of the suborder Spongillina. Pachydictyum globosum (Malawispongiidae) and Nudospongilla vasta (Spongillidae), two morphologically quite distinct species from Sulawesi were found in a joint clade with Trochospongilla (Spongillidae) rendering Trochospongilla paraphyletic. Furthermore, Ochridaspongia sp., another Malawispongiidae, clustered far away from that clade, together with Ephydatia fluviatilis, making the latter family polyphyletic. The Lubomirskiidae endemic to Lake Baikal, Lubomirskia abietina, Baikalospongia bacillifera, B. intermedia, and Swartschewskia papyracea formed a well-supported clade that was most closely linked to the genus Ephydatia (99.9% identity over a total length of 2169 concatenated nucleotide positions). Our study indicates the frequent and independent origin of sponge species endemic to different freshwater ecosystems from a few cosmopolitan founder species. The highly specific primer sets newly developed here facilitate work on the molecular phylogeny and DNA barcoding of sponges. 相似文献
80.
1. A survey of lakes in Anglesey and Snowdonia, Wales, in the summer of 1997 recorded changes in the triclad fauna which had occurred since earlier studies in the 1950s and in 1973. Two native species, Polycelis tenuis and Dugesia polychroa, and two immigrants, Planaria torva and especially Dugesia tigrina, have increased their range. The triclad fauna has remained unchanged with time in eleven out of sixteen lakes in Snowdonia, in contrast to only one out of fourteen lakes on Anglesey. This supports the hypothesis produced in the 1950s, that the triclad fauna of the ion-poor, unproductive lakes in Snowdonia would change little with time, being maintained by ecological interactions, whereas the ion-rich, productive lakes on Anglesey, from which native Dugesia spp. and Dendrocoelum lacteum are historically absent, would increase in triclad diversity. 2. An examination of national records for the distribution of D. tigrina and Pl. torva indicated their continuing dispersal in both still and running waters in mainland Britain, with the former species having the greater frequency of occurrence. 3. The reasons for this may be that D. tigrina is (a) more easily dispersed, (b) a more opportunistic, catholic feeder, (c) a more vigorous competitor, (d) able to reproduce asexually and (e) able to prey to some extent on other triclad species, as shown in the laboratory but not yet confirmed in the field. The absence of D. tigrina and Pl. torva from unproductive lakes may be explained in terms of low temperature and feeding mode, and a low standing crop of gastropods, respectively. 4. Seven surveys (1961–1997) of the triclad fauna (six species) of Colemere, England, revealed that D. tigrina was confined to one small area of the littoral zone until the mid-1980s, after which it spread fairly rapidly to occupy the entire shore by 1997. Its expansion has been to the detriment of all the native species, particularly P. tenuis, except Dd. lacteum. The possible extinction of Pl. torva may be linked to a numerical reduction in snails, particularly Potamopyrgus jenkinsi. 5. Dugesia tigrina has an adverse effect on the native triclad fauna in lakes, particularly when only a few species are present. This has been explained in terms of the availability of a wider variety of food. The presence of a larger number of triclad species denies the immigrant food items, particularly crustaceans and snails, the food refuges of Dd. lacteum and Dugesia spp., respectively. However, the long-term study of Colemere has shown that more diverse triclad faunas may also be vulnerable to this immigrant. The effect of Pl. torva on native triclads remains uncertain. 相似文献