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11.
Role of cellular antioxidants in metal-induced damage 总被引:23,自引:0,他引:23
M. Sugiyama 《Cell biology and toxicology》1994,10(1):1-22
12.
Escherichia coli, genetically engineered with a mercury(II)-sensitive promoter and the lux genes from Vibrio fischeri, were used as microbial bioluminescent sensors for the detection of mercury. Evaluation of this genetic construction was carried out by determining the effects of various parameters on cell suspensions maintained at constant conditions in a small 100-mL vessel. The strongest light intensities and quickest induction times occurred with cells in the midexponential growth phase maintained at 28 degrees C, concentrated to 1 x 10(9) cells/mL, mixed at very fast speeds, and aerated at 2 vvm (volume of air per volume of culture per minute) during light measurement in the small vessel. The cells were sensitive to the mercuric ion in the range of 20 nM to 4 muM (4 to 800 ppb), and the total response time was on the order of 1 hour, depending on the above parameters. The cells exhibited great specificity for mercury. The cells had almost equal specificity for organic and inorganic forms of the mercuric ion and responded more weakly to the mercurous ion. A simple, inexpensive, durable miniature probe (3 mL) was constructed and operated using the optimum parameters found in the small vessel as a guide. The range of sensitivity to the mercuric ion detected in the probe was 10 nM to 4 muM when aeration was provided. (c) 1993 John Wiley & Sons, Inc. 相似文献
13.
14.
Mercury evaporation from undisturbed iron‐humus podzol lysimeters was measured over 3 months after treatment with HgCl2 spiked with radioactive 203Hg. The relative evaporation rate from HgCl2 treated soils followed the sum of two exponential functions. Because evaporation asymptotically approaches zero with time, the integral of the fit curve represents the evaporative loss in percent of atmospheric deposition. For the soil investigated, about 5% of atmospheric Hg deposition was reemitted into the atmosphere. It is hypothesized that mercury evaporation can decrease the leaching of mercury in and from soil significantly; this effect is probably increasing with decreasing rain acidity or soil acidity. Mercury deposited as soluble salt remains susceptible to reemission to air for 300 d after incorporation into the soil matrix. Indications are found that Hg evaporation from soils in geological background areas predominantly derives from recent atmospheric Hg deposition and not from geological sources. 相似文献
15.
Paul Allen 《Biological trace element research》1995,50(3):193-208
The interaction of mercury and cadmium with lead was investigated by exposingOreochromis aureus to two heavy metals simulataneously. The chronic accumulation prolife of lead was determined by analyzing the liver, brain,
gill filaments, intestine, caudal muscle, spleen, trunk kidney, and gonads following exposure to lead alone and in mixtures
with mercury and cadmium. Nominal exposure concentrations of lead were 0.05, 0.10, 0.50, and 1.00 mg/L. Mixtures of lead (0.50
or 0.05 mg/L) with cadmium (0.05 mg/L) and lead (0.50 or 0.05 mg/L) with mercury (0.05 mg/L) were also used. Following 140
d of exposure to lead, the highest concentrations of lead consistently accumulated in the trunk kidney. The concentration
of lead in the kidney was decreased by coexposure to mercury or cadmium, but increased in the muscle and liver. Under all
exposure regimes, the median concentration of lead in the muscle exceeded safety levels recommended for human consumption.
In a food fish, such asO. aureus, a knowledge of toxic metal accumulation patterns is of great importance. 相似文献
16.
The effects of inorganic mercury (HgII) and methylmercury (MeHg) on the colonization of artificial substrates by periphytic
diatoms were studied using indoor freshwater microcosms. These consisted of a mixed biotope– water column + natural sediment
– with rooted macrophyte cuttings (Elodea densa) and benthic bivalve molluscs (Corbicula fluminea).The periphyton was collected
on glass slides in the water column after 34and 71 days. The two Hg sources were introduced either by daily additions to the
water column, or once at the beginning into the sediment, using two nominal concentrations: water column, 0.5 μgL-1 and 2 μg L-1 for both compounds: sediment, 0.5 mg kg-1 (fw) and 2 mgkg-1 (fw) for MeHg and 1 mg kg-1 (fw) and 10 mgkg-1 (fw) for HgII. Several complementary criteria were used to analyse the structural and functional perturbations induced: cell
density, species richness, diatom size, relative abundance. Exposure to MeHg added to the water column resulted in reduced
cell density and changes in species composition with enhancement of e.g. Fallacia pygmaea or Nitzschia palea; inorganic Hg
had less effect on the population structure. After contamination via the sediment, the effects of the two compounds were less
pronounced than for the water source.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
17.
The excretion of mercury, copper and zinc in urine, and mercury in whole blood andplasma, was determined in 40 chloralkali workers exposed to mercury vapour and 40age-matched referents. The Hg concentrations in whole blood, plasma and urine werehigher in the exposed group (35 nmol l, 30 nmol l,and 11.5 nmol mmol creatinine, respectively) in comparison with thereference group (15 nmol l, 6.3 nmol l, and 1.8nmol mmol creatinine, respectively). The urinary copper excretionwas similar in the two groups, while U-Zn excretion was significantly higher (P = 0.04)in the exposed group, median 0.83 mmol mmol creatinine versus 0.76mnmol mmol creatinine in the reference group. In a subgroup of exposedworkers with current U-Hg above 11.5 nmol lmmolcreatinine (20 mg g creatinine) the medianU-Zn was 1.1 mmol mmol creatinine. In both groups smokers had highU-Zn levels than non smokers. When both U-Hg and smoking were taken into account in alinear regression model, there was a significant association between U-Hg and U-Zn inthe combined group of exposed and referents (P = 0.002). This study indicates thatmercury exposure in humans, as in animals, causes increased urinary excretion of zinc.The mechanisms may be induced synthesis of metallothionein in the kidneys, displacementof Zn from preexisting metallothionein by Hg, or a decreased reabsorption of zinc in thekidneys owing to a slight tubular dysfunction. 相似文献
18.
蛋白质晶体的优化生长 总被引:3,自引:0,他引:3
蛋白质晶体的优化生长是获得高质量蛋白质晶体, 进而得到高精度晶体结构的有效途径.针对不同的晶体生长方法,已尝试了不同的优化手段,这对改善某些蛋白质晶体的质量显示了明显的成效.然而,鉴于蛋白质晶体生长的多样性与复杂性,这些方面均未发展成为实用的技术.文章综述了这类研究进展,分析了各手段的利弊,并指出了应着重解决的问题. 相似文献
19.
Andrzej J. Żelazowski Jadwiga A. Szymańska Czesklaw S. Cierniewski 《Chemico-biological interactions》1980,33(1):115-125
Two homogenous fractions of hepatic metallothioneins ((Cd,Zn) MT-1 and (Cd,Zn) MT-2) and renal metal binding proteins ((Bi,Cu) BP-1 and (Bi,Cu) BP-2) were isolated from rats exposed to heavy metals and specific antisera to them were produced in rabbits.These antisera were tested by immunodiffusion and immunoelectrophoresis for their ability to bind different fractions of hepatic Cd,Zn -metallothionein and renal (Bi,Cu)-, (Hg,Cu)- and (Cd,Cu)-binding proteins. It was found that anti (Bi,Cu) BP antisera did not cross-react with hepatic (Cd,Zn) MT-1 and (Cd,Zn) MT-2. Strong immunological cross-reactions were detected between anti (Bi,Cu) BP antisera and individual forms of (Cd,Cu)-, (Hg,Cu)- and (Bi,Cu)-binding proteins isolated from rat kidneys. 相似文献
20.
Dissolved and suspended mercury. species in the Wabigoon River (Ontario,Canada): seasonal and regional variations 总被引:1,自引:1,他引:0
Jackson T. A. Parks J. W. Jones P. D. Woychuk R. N. Sutton J. A. Hollinger J. D. 《Hydrobiologia》1982,91(1):473-487
Seasonal and regional variations in the speciation, sediment-water partitioning, and dynamics of mercury (Hg) were studied
at selected sites along the Hg-polluted Wabigoon River, and at unpolluted headwater and tributary sites, during April–September,
1979. ‘Dissolved’ and ‘particulate’ forms of Hg in the water were separated by continuous-flow centrifugation in the field.
The Hg and other pollutants such as wood chips and salt had been discharged from a chlor-alkali plant and paper mill at Dryden,
Ontario.
Concentrations and loadings of particulate methyl mercury (CH3Hg+) and total particulate Hg (and loadings of total ‘dissolved’ Hg) were greatest during the spring flood (April-May) owing
to accelerated resuspension and transport of sediments. Concentrations of ‘dissolved’ CH3Hg+, however, were highest in the summer (July–September), probably reflecting stimulation of microbial methylating activity
by elevated temperatures, together with factors such as reduced levels of metal-scavenging particulates and minimal dilution
by runoff. Total dissolved Hg concentrations were relatively high in September at polluted sites only, possibly because of
desorption from sediments due to elevated concentrations of Cl− ions. Loadings of dissolved CH3Hg+ tended to be high in the summer but were generally depressed (suggesting sorption by suspended particles) during the major
spring-flood episode in May.
During July–August dissolved CH3Hg+ was a function of total dissolved Hg, suggesting rapid biomethylation of desorbed inorganic Hg; but in general dissolved
and suspended CH3Hg+ levels depended on environmental variables and were unrelated to total Hg concentrations. In the summer only, total dissolved
Hg was a function of dissolved Cl−. Hg species in particulates were associated with sulfides, hydrated Fe and Mn oxides, organic matter (notably high molecular
weight humic and humic-Fe components), and selenium (Se); but CH3Hg+ and total Hg differed in their specific preferences for binding agents, implying that binding sites discriminate between
CH3Hg+ and Hg2+ ions. CH3Hg+ was associated with sulfide and (in the spring only) with Fe oxides, whereas total Hg was associated with organic matter
and Se and with DTPA- and NaOH-extractable Fe in the spring but with Mn oxide and NaOH-extractable organics in the summer.
Sulfides were most abundant in May, indicating that they were eroded from bottom sediments, but Fe and Mn oxides were most
abundant in the summer, probably owing to activities of filamentous iron bacteria and other micro-organisms.
Particulate Hg was 98–100% nonextractable by mild solvents such as Ca acetate, CaCl2, dilute acetic acid, and (at polluted sites only) DTPA solutions, suggesting that the particulate Hg mobilized in the spring
may not be readily available to organisms; association with Se and high molecular weight humic matter also supports this hypothesis.
Hg probably becomes more bio-available in the summer, as suggested by the upsurge in dissolved CH3Hg+ and total dissolved Hg levels, and by increases in the solubility of particulate Hg in acetic acid, DTPA, H2O2, and NaOH solutions, as well as an increase in the relative importance of lower molecular weight fractions of NaOH-extractable
Hg (in September).
Regional variations in Hg speciation and partitioning reflected a gradient in sediment composition from wood chips near Dryden
to silt-clay mud further downstream. Hg in silt-clay mud relatively far (> 35 km) downstream from the source of pollution
or in unpolluted areas appeared to be more readily solubilized by Cl− ions or chelators such as DTPA, more readily methylated (as indicated by downstream increases in dissolved CH3Hg+ levels and CH3Hg+/total Hg ratios), and was to a greater degree organically bound (H2O2-extractable), and thus was probably more bio-available, than Hg in wood-chip deposits. Possible explanations include weaker
binding of Hg by the mud, the more finely divided state of the mud, and improved microbial growth at lower concentrations
of toxic pollutants. Owing to enrichment in sulfides and Fe oxides, resuspended wood-chip sediments were especially efficient
scavengers of CH3Hg+.
The results indicate that in any pollution abatement plan aimed at lowering the Hg levels in the biota of lakes fed by the
Wabigoon River, immobilization, removal, or detoxification of dissolved as well as particulate forms of Hg in the river would
probably have to be considered. Possibly, Hg species could be ‘scrubbed’ from the river water by increasing the suspended
load and by sedimentation and treatment with Hg-binding agents in special receiving basins. 相似文献