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21.
22.
Colony formation by variant Chinese hamster cells highly resistant to adenine analogs and deficient in adenine phosphoribosyltransferase (APRT) activity was measured after co-cultivation with APRT+, CHO-K1 cells in medium containing one of three different adenine analogs. Depending upon the density of APRT+ cells and the specific adenine analog, large differences in the recovery of APRT? colonies were observed. The particular adenine analog and APRT+ cell density were more significant factors in the recovery of APRT? colonies than the concentration of the analog or the level of APRT activity. The number of wild-type cells (CHO-K1) required to inhibit formation of APRT? colonies by 50% (mean lethal density; MLD50) with 65 μg/ml 8-aza-adenine (AzA) as the selective drug was 8.0 × 105 cells/100 mm dish (1.5 × 104/cm2). With 100 μg/ml 2,6-diaminopurine (DAP) the MLD50 for CHO-K1 was 4.0 × 105 cells/100 mm dish (7.3 × 103/cm2). The MLD50 for CHO-K1 when the DAP concentration was decreased to 50 μg/ml was only slightly higher, 5 × 105 cells/100 mm dish (9.1 × 103/cm2). The most toxic effect was observed with 2-fluoroadenine (FA). The MLD50 for CHO-K1 in 2 μg/ml FA was 4.5 × 104 cells/100 mm dish (8.2 × 102/cm2), a cell density which permits minimal direct contact between APRT+ and APRT? cells. The toxic effects of FA on individually resistant, APRT? cells were found to be mediated by metabolites released into the medium by dying APRT+ cells. This metabolite toxicity to APRT? cells was also demonstrated in mixtures with cells having only 8% of wild-type APRT activity. The MLD50 for these APRT+ (8%) cells in 2 μg/ml FA was 7.5 × 104 cells/100 dish (1.4 × 103/cm2), a small difference from the MLD50 for cells with wild-type levels of APRT activity. The differences in the recovery of APRT? colonies from mixtures with APRT+ cells in these three adenine analogs are critical to the design of procedures for the selection of APRT? cells from populations of APRT+ cells and emphasize the importance of establishing the parameters of metabolic cooperation, not only in terms of cell density but also with regard to the particular selective agent, in any experiment designed to determine precise mutation rates or to test putative mutagens upon mammalian cells in culture. 相似文献
23.
Résumé Avant d'entrer en contact, les surfaces de fusion des processus palatins subissent des modifications. Certaines cellules épithéliales superficielles deviennent turgescentes, alors que d'autres conservent un aspect dense et aplati, elles se lysent et éclatent. Des noyaux semblables à ceux des assises profondes se retrouvent en surface, paraissant migrer vers le bourgeon antagoniste. Des projections filamenteuses apparaissent, quelques unes établissant un contact avec l'épithélium opposé, d'autres restant seulement sous forme d'expansion. Ces observations sont concordantes en microscopie photonique et en microscopie électronique à balayage.
Modification of the medial epithelium of the palatal shelves of mice at the prefusion stage. A light and scanning electron microscopy study
Summary The fusion surfaces of the palatal processes are modified before coming into contact. Several superficial epithelial cells become hypertrophied, undergo lysis and burst, whereas others remain flattened and densely stained. Nuclei, similar to those localized in deeper layers are found on the surface and seem to migrate towards the opposite process. Filamentous projections appear and some establish a linkage with the opposite epithelium, whereas others expand freely from the surface. Light microscope observations agree with scanning electron microscopy findings.相似文献
24.
In order to study the variability in nutrient concentrations in four tissues of Q. ilex in relation to soil properties, we selected fifteen stands in both Quercus ilex forests and Q. ilex-Pinus halepensis mixed forests. These stands had developed on soils derived from eight different parent materials. Three soil groups were differentiated according to their chemical properties: calcareous soils, siliceous soils, and volcanic soils. Across sites, nutrient concentrations were generally less variable in current-year tissues than in older tissues. Nitrogen and potassium showed the lowest variability among sites, their concentrations in current-year leaves ranging from 1.17% to 1.39% for N and from 0.53% to 0.68% for K. There were few statistically significant correlations between tissue element concentrations, the most frequent being the antagonistic relationship between calcium and magnesium. Nitrogen concentration in current-year leaves was negatively correlated with soil chemical fertility (nitrogen, phosphorus and potassium). This may reflect a nutritional imbalance between nitrogen and other nutrients, some of which may be more limiting than nitrogen to Q. ilex growth in Catalonia forests. Negative correlations were also found between plant magnesium and soil calcium, and positive correlations between plant calcium and soil calcium. 相似文献
25.
A common sequence in the inverted terminal repetitions of human and avian adenoviruses 总被引:8,自引:0,他引:8
The termini of the avian chick embryo lethal orphan (CELO) virus DNA have been sequenced. The results revealed a 63-bp-long inverted terminal repetition (ITR) which shared the sequence ATAATA with all adenovirus termini, thus far analyzed. The CELO virus ITR differed from those of the mammalian adenoviruses in two major aspects: (i) it is not a perfect duplication; (ii) it begins with a 5'-guanylic acid residue instead of the cytidylic acid normally observed in adenoviruses. 相似文献
26.
R. A. E. Tilney-Bassett Dr. O. A. L. Abdel-Wahab 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1982,62(2):185-191
Summary Two distinct segregation patterns are recognized after G X W plastid crosses in Pelargonium. Type I parents produce offspring in which maternal zygotes are frequent, biparental intermediate, and paternal zygotes rare (MZ>BPZ>PZ), as defined by the presence or absence of green or white plastids in the young embryos into which the zygotes develop. Type II parents produce offspring in which maternal and paternal zygotes are frequent with biparental zygotes the least frequent class (MZ>BPZPr
1
Pr
1. Type II plants, which do not breed true, are regarded as heterozygotes — Pr
1
Pr
2. The nuclear gene is symbolized as Pr as it is presumed to control alternative patterns of plastid segregation through an effect on plastid replication.Selfs and intercrosses of heterozygous plants segregate in an unexpected 1:1 ratio and not the expected 3:1 (1:2:1). The alternative homozygote — Pr
2
Pr
2 — could not be detected. Reciprocal crosses between heterozygotes (Pr
1
Pr
2) and homozygotes (Pr
1
Pr
1) give the expected 1:1 ratio when the Pr
2 allele is derived from the male, whereas there is often, but not always, a highly significant deviation from 1:1 when the Pr
2 allele is derived from the female.A simple explanation, which is not wholly satisfactory, is to assume that Pr
2 is a gametophytic lethal on the female side. An alternative, or additional, explanation is that an incompatibility mechanism is involved in which Pr
1 is a self-compatible allele, Pr
2 a self-incompatible allele, and Pr
1-Pr
2 cross-compatible alleles. Successful fertilization is then determined by sporophytic control on the male side and gametophytic control on the female side. 相似文献
27.
Milena Sedliaková Viera Slezáriková Jela Brozmanová František Mašek Viktória Bayerová 《Mutation research》1980,71(1):15-23
3 wild-type strains of E. coli, namely K12 AB2497, B/r WP2 and 15 555-7v proficient in excision and post-replication repair, differ markedly in their UV resistance. To elucidate this difference, the influence was investigated of induction by application of inducing fluence (IF) before lethal fluence (LF) on repair processes after LF. In cells distinguished by low UV resistance (E. coli 15 555-7; E. coli B/r WP2), dimer excision was less complete in cultures irradiated with IF + LF than in cultures irradiated with LF only. The highly resistant E. coli K12 AB2497 performed complete excision both after IF + LF or after LF alone. All 3 types of cell survived better after IF + LF than after LF only. Because, in most strains so far investigated, the application of IF reduced dimer excision and increased survival, dimer excision per se does not appear important for survival.We conclude that the rate and completeness of dimer excision can serve as a measure of efficiency of the excision system whose action is necessary for repair of another lesion. Cells of all investigated strains could not resume DNA replication and died progressively when irradiated with LF and post-incubated with chloramphenicol (LF CAP+). Thus, it appears that inducible proteins are necessary for repair in all wild-type E. coli cells give with potentially lethal doses of UV irradiation. 相似文献
28.
The effect of pre- and posttreatment incubation of UV-irradiated and ethyl methanesulphonate (EMS) treated cells with non-toxic concentrations of inhibitors of de novo purine synthesis (dnPS) on expression of potentially lethal and premutational damage at the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in V79 cells has been examined. The concentrations of inhibitors used were shown to profoundly perturb de novo DNA synthesis, by measurements of [14C]formate uptake, and cell cycle progression by flow cytofluorimetry. Postincubation in 6-methyl mercaptopurine ribonucleoside (MMPR) usually but not invariably potentiated the cytotoxic effects of UV and EMS but azaserine (AZS) and methotrexate (MTX) were without effect. No effects on mutant frequencies were observed on posttreatment with any of these agents. Caffeine produced the least effect on dnPS, but invariably potentiated lethal damage. This potentiation of lethal damage is not mediated by dnPS inhibition as has been suggested for Chinese hamster ovary (CHO) cells. 相似文献
29.
Judi E Hewitt Conrad A Pilditch 《Journal of experimental marine biology and ecology》2004,306(1):95-112
Epifaunal suspension-feeding bivalves can play important roles in marine ecosystems affecting macrobenthic communities, benthic boundary layers and benthic-pelagic coupling, not just by their presence but also by any changes in feeding behaviour. While seston quality and quantity have consistently been found to be important influences on the feeding rates of suspension-feeding bivalves, factors stressing individuals are also likely to be important, as they may affect energy-dependent thresholds of response. We postulated that (1) history of seston quantity would affect how suspension feeders deal with increases in total suspended particulates, and (2) high-seston concentrations would affect feeding rates more in individuals whose energy reserves were low after spawning. Three sites were selected for short-term (1 day) feeding experiments on the pinnid bivalve, Atrina zelandica. At one site, the experiment was run pre-and postspawning. Atrina exhibited high rejection of filtered particles (mostly 75% to 100%) and high organic absorption efficiencies (0.9-1) at all seston levels. Strong differences in the response of feeding behaviour to increased seston concentrations were observed between A. zelandica from the different sites, with lesser differences observed between times. The site-specific feeding responses to seston concentrations observed are likely to affect our ability to model responses of A. zelandica to sediment loading and to influence the importance of A. zelandica to benthic-pelagic coupling. 相似文献
30.
Comparison of the Virulence of Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus
Sadao Mizobuchi Junzaburo Minami Fu Jin Osamu Matsushita Akinobu Okabe 《Microbiology and immunology》1994,38(8):599-605
The virulence of methicillin-resistant Staphylococcus aureus (MRSA) was compared with that of methicillin-sensitive S. aureus (MSSA), using 13 MRSA and 7 MSSA strains isolated from clinical specimens. The infectivity and lethality of the two groups were examined as to the inoculum required to infect 50% of guinea pigs (ID50) and to kill 50% of mice (LD50), respectively. The mean ID50 [log10 colony forming units (CFU)] for MRSA strains was 7.1 ± 0.60 standard deviation, which was 1.5 higher than that for MSSA strains (P < 0.001). The mean LD50 (log10 CFU) for MRSA strains was 9.0 ± 0.42, being 1.1 higher than that for MSSA strains (P = 0.001). Pretreatment of mice with cyclophosphamide decreased the mean LD50 for MRSA strains more than that for MSSA strains, resulting in the difference in the mean LD50 being insignificant (P = 0.502). These results indicate that MRSA is less virulent than MSSA in normal hosts, but that they are equally virulent in immunocompromised hosts. The growth of MRSA strains was much slower than that of MSSA strains in the lag phase, although their growth rates were almost the same in the exponential growth phase, suggesting that the difference in virulence between them may be at least partly due to such a difference in growth. 相似文献