全文获取类型
收费全文 | 8199篇 |
免费 | 1094篇 |
国内免费 | 1239篇 |
出版年
2024年 | 36篇 |
2023年 | 217篇 |
2022年 | 253篇 |
2021年 | 363篇 |
2020年 | 522篇 |
2019年 | 558篇 |
2018年 | 486篇 |
2017年 | 513篇 |
2016年 | 496篇 |
2015年 | 447篇 |
2014年 | 510篇 |
2013年 | 625篇 |
2012年 | 383篇 |
2011年 | 441篇 |
2010年 | 382篇 |
2009年 | 430篇 |
2008年 | 370篇 |
2007年 | 414篇 |
2006年 | 377篇 |
2005年 | 337篇 |
2004年 | 238篇 |
2003年 | 250篇 |
2002年 | 216篇 |
2001年 | 197篇 |
2000年 | 165篇 |
1999年 | 152篇 |
1998年 | 136篇 |
1997年 | 119篇 |
1996年 | 82篇 |
1995年 | 88篇 |
1994年 | 70篇 |
1993年 | 76篇 |
1992年 | 74篇 |
1991年 | 48篇 |
1990年 | 49篇 |
1989年 | 31篇 |
1988年 | 29篇 |
1987年 | 25篇 |
1986年 | 18篇 |
1985年 | 45篇 |
1984年 | 80篇 |
1983年 | 36篇 |
1982年 | 55篇 |
1981年 | 34篇 |
1980年 | 23篇 |
1979年 | 9篇 |
1978年 | 10篇 |
1977年 | 5篇 |
1976年 | 3篇 |
1975年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
941.
M. J. Kennard B. J. Pusey A. H. Arthington B. D. Harch S. J. Mackay 《Hydrobiologia》2006,572(1):33-57
Multivariate predictive models are widely used tools for assessment of aquatic ecosystem health and models have been successfully
developed for the prediction and assessment of aquatic macroinvertebrates, diatoms, local stream habitat features and fish.
We evaluated the ability of a modelling method based on the River InVertebrate Prediction and Classification System (RIVPACS)
to accurately predict freshwater fish assemblage composition and assess aquatic ecosystem health in rivers and streams of
south-eastern Queensland, Australia. The predictive model was developed, validated and tested in a region of comparatively
high environmental variability due to the unpredictable nature of rainfall and river discharge. The model was concluded to
provide sufficiently accurate and precise predictions of species composition and was sensitive enough to distinguish test
sites impacted by several common types of human disturbance (particularly impacts associated with catchment land use and associated
local riparian, in-stream habitat and water quality degradation). The total number of fish species available for prediction
was low in comparison to similar applications of multivariate predictive models based on other indicator groups, yet the accuracy
and precision of our model was comparable to outcomes from such studies. In addition, our model developed for sites sampled
on one occasion and in one season only (winter), was able to accurately predict fish assemblage composition at sites sampled
during other seasons and years, provided that they were not subject to unusually extreme environmental conditions (e.g. extended
periods of low flow that restricted fish movement or resulted in habitat desiccation and local fish extinctions). 相似文献
942.
943.
Samantha Jane Hughes 《Hydrobiologia》2006,553(1):27-41
Temporal and spatial distribution patterns of lotic larval trichopteran assemblages in relation to environmental variables
were investigated in Madeiran streams using multivariate analyses. TWINSPAN classification detected distinct faunal assemblages
related to spatial factors between non-polluted high altitude sites and lower lying enriched sites where tolerant taxa were
predominant but showed strong seasonal shifts in species composition and abundance. The 15 TWINSPAN end groups were grouped
into five arbitrary clusters based upon the seasonal and spatial changes in the trichopteran assemblages detected by the analysis.
Significant differences between environmental variables (distance from source, altitude, temperature, conductivity, alkalinity
and nitrate) and the trichopteran assemblages (using trichopteran based metrics) of these clusters were confirmed by the Kruskal-Wallis
test (H) and Dunn’s test. Chemical classification of samples within the clusters revealed a strong association between trichopteran
assemblages and water quality. Canonical Correspondence Analysis and Monte Carlo global permutation tests also identified
significant associations between the larval assemblages and physicochemical variables such as temperature and conductivity
along a strong physical gradient (altitude, slope) and nitrate along a weaker seasonal gradient. Analysis of functional feeding
group distribution patterns clearly showed that mid to high altitude indigenous woodland sites were trophically diverse whilst
the lower reaches of the islands streams are trophically impoverished with strong seasonal shifts between two feeding groups
of enrichment tolerant taxa. Trichopteran shredders are exclusive to indigenous woodland sites, indicating a limited distribution
associated with land use, allochthonous input and habitat destruction. The results indicate that several ‘environmental filters’
operate at different levels upon the islands trichopteran fauna, producing temporally and spatially distinct ‘subsets’ of
species best able to exploit conditions and resources at a given site or time, confounding the direct comparison of these
insular systems with the findings of the River Continuum Concept, traditionally associated with unaffected continental lotic
systems. 相似文献
944.
Interactive effects of environmental stress and inbreeding on reproductive traits in a wild bird population 总被引:3,自引:0,他引:3
1. Conservation biologists are concerned about the interactive effects of environmental stress and inbreeding because such interactions could affect the dynamics and extinction risk of small and isolated populations, but few studies have tested for these interactions in nature. 2. We used data from the long-term population study of song sparrows Melospiza melodia on Mandarte Island to examine the joint effects of inbreeding and environmental stress on four fitness traits that are known to be affected by the inbreeding level of adult birds: hatching success, laying date, male mating success and fledgling survival. 3. We found that inbreeding depression interacted with environmental stress to reduce hatching success in the nests of inbred females during periods of rain. 4. For laying date, we found equivocal support for an interaction between parental inbreeding and environmental stress. In this case, however, inbred females experienced less inbreeding depression in more stressful, cooler years. 5. For two other traits, we found no evidence that the strength of inbreeding depression varied with environmental stress. First, mated males fathered fewer nests per season if inbred or if the ratio of males to females in the population was high, but inbreeding depression did not depend on sex ratio. Second, fledglings survived poorly during rainy periods and if their father was inbred, but the effects of paternal inbreeding and rain did not interact. 6. Thus, even for a single species, interactions between the inbreeding level and environmental stress may not occur in all traits affected by inbreeding depression, and interactions that do occur will not always act synergistically to further decrease fitness. 相似文献
945.
Antimicrobial peptides temporins B and L induce formation of tubular lipid protrusions from supported phospholipid bilayers
下载免费PDF全文
![点击此处可从《Biophysical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The binding of the antimicrobial peptides temporins B and L to supported lipid bilayer (SLB) model membranes composed of phosphatidylcholine and phosphatidylglycerol (4:1, mol/mol) caused the formation of fibrillar protrusions, visible by fluorescent microscopy of both a fluorescent lipid analog and a labeled peptide. Multicolor imaging at low peptide-to-lipid ratios (P/L < approximately 1:5) revealed an initial in-plane segregation of membrane-bound peptide and partial exclusion of lipid from the peptide-enriched areas. Subsequently, at higher P/L numerous flexible lipid fibrils were seen growing from the areas enriched in lipid. The fibrils have diameters <250 nm and lengths of up to approximately 1 mm. Fibril formation reduces the in-plane heterogeneity and results in a relatively even redistribution of bound peptide over the planar bilayer and the fibrils. Physical properties of the lipid fibrils suggest that they have a tubular structure. Our data demonstrate that the peptide-lipid interactions alone can provide a driving force for the spontaneous membrane shape transformations leading to tubule outgrowth and elongation. Further experiments revealed the importance of positive curvature strain in the tubulation process as well as the sufficient positive charge on the peptide (>/=+2). The observed membrane transformations could provide a simplified in vitro model for morphogenesis of intracellular tubular structures and intercellular connections. 相似文献
946.
Abdel-Mottaleb Y Clynen E Jalali A Bosmans F Vatanpour H Schoofs L Tytgat J 《FEBS letters》2006,580(26):6254-6258
The very first member of K(+) channels toxins from the venom of the Iranian scorpion Odonthobuthus doriae (OdK1) was purified, sequenced and characterized physiologically. OdK1 has 29 amino acids, six conserved cysteines and a pI value of 4.95. Based on multiple sequence alignments, OdK1 was classified as alpha-KTx 8.5. The pharmacological effects of OdK1 were studied on six different cloned K(+) channels (vertebrate Kv1.1-Kv1.5 and Shaker IR) expressed in Xenopus laevis oocytes. Interestingly, OdK1 selectively inhibited the currents through Kv1.2 channels with an IC50 value of 183+/-3 nM but did not affect any of the other channels. 相似文献
947.
Lehmann L Perrin N Rousset F 《Evolution; international journal of organic evolution》2006,60(6):1137-1151
Limited dispersal may favor the evolution of helping behaviors between relatives as it increases their relatedness, and it may inhibit such evolution as it increases local competition between these relatives. Here, we explore one way out of this dilemma: if the helping behavior allows groups to expand in size, then the kin-competition pressure opposing its evolution can be greatly reduced. We explore the effects of two kinds of stochasticity allowing for such deme expansion. First, we study the evolution of helping under environmental stochasticity that may induce complete patch extinction. Helping evolves if it results in a decrease in the probability of extinction or if it enhances the rate of patch recolonization through propagules formed by fission of nonextinct groups. This mode of dispersal is indeed commonly found in social species. Second, we consider the evolution of helping in the presence of demographic stochasticity. When fecundity is below its value maximizing deme size (undersaturation), helping evolves, but under stringent conditions unless positive density dependence (Allee effect) interferes with demographic stochasticity. When fecundity is above its value maximizing deme size (oversaturation), helping may also evolve, but only if it reduces negative density-dependent competition. 相似文献
948.
Abrams PA 《Evolution; international journal of organic evolution》2006,60(3):427-439
Mathematical models of consumer-resource systems are used to explore the evolution of traits related to resource acquisition in a generalist consumer species that is capable of exploiting two resources. The analysis focuses on whether evolution of traits determining the capture rates of two resources by a consumer species produce one generalist, two specialists, or all three types, when all types are characterized by a common fitness function. In systems with a stable equilibrium, evolution produces one generalist or two specialists, depending on the second derivative of the trade-off relationship. When there are sustained population fluctuations, the nature of the trade-off between the consumer's capture rates of the two resources still plays a key role in determining the evolutionary outcome. If the trade-off is described by a choice variable between zero and one that is raised to a power n, polymorphic states are possible when n > 1, which implies a positive second derivative of the curve. These states are either dimorphism, with two relatively specialized consumer types, or trimorphism, with a single generalist type and two specialists. Both endogenously driven consumer-resource cycles, and fluctuations driven by an environmental variable affecting resource growth are considered. Trimorphic evolutionary outcomes are relatively common in the case of endogenous cycles. In contrast to a previous study, these trimorphisms can often evolve even when new lineages are constrained to have phenotypes very similar to existing lineages. Exogenous cycles driven by environmental variation in resource growth rates appear to be much less likely to produce a mixture of generalists and specialists than are endogenous consumer-resource cycles. 相似文献
949.
The designed antimicrobial peptide KIGAKIKIGAKIKIGAKI possesses enhanced membrane selectivity for bacterial lipids, such as phosphatidylethanolamine and phosphatidylglycerol. The perturbation of the bilayer by the peptide was first monitored using oriented bilayer samples on glass plates. The alignment of POPE/POPG model membranes with respect to the bilayer normal was severely altered at 4 mol% KIGAKI while the alignment of POPC bilayers was retained. The interaction mechanism between the peptide and POPE/POPG bilayers was investigated by carefully comparing three bilayer MLV samples (POPE bilayers, POPG bilayers, and POPE/POPG 4/1 bilayers). KIGAKI induces the formation of an isotropic phase for POPE/POPG bilayers, but only a slight change in the 31P NMR CSA line shape for both POPE and POPG bilayers, indicating the synergistic roles of POPE and POPG lipids in the disruption of the membrane structure by KIGAKI. 2H NMR powder spectra show no reduction of the lipid chain order for both POPG and POPE/POPG bilayers upon peptide incorporation, supporting the evidence that the peptide acts as a surface peptide. 31P longitudinal relaxation studies confirmed that different dynamic changes occurred upon interaction of the peptide with the three different lipid bilayers, indicating that the strong electrostatic interaction between the cationic peptide KIGAKI and anionic POPG lipids is not the only factor in determining the antimicrobial activity. Furthermore, 31P and 2H NMR powder spectra demonstrated a change in membrane characteristics upon mixing of POPE and POPG lipids. The interaction between different lipids, such as POPE and POPG, in the mixed bilayers may provide the molecular basis for the KIGAKI carpet mechanism in the permeation of the membrane. 相似文献
950.
Many promising therapeutics are currently awaiting their clinical application. Due to their low capability of cell membrane crossing, these compounds do not reach their site of action. One way to overcome this problem might be the fusion of these agents to cell-penetrating peptides (CPP), which are able to shuttle various cargoes across cellular membranes. One disadvantage in using CPP in drug delivery is their low metabolic stability. The aim of our work was to increase the proteolytic resistance of the CPP hCT(9-32), a truncated C-terminal fragment of human calcitonin. Thus, we synthesised six modified N-terminally carboxyfluorescein labelled hCT(9-32) derivatives by replacing positions 12 and/or 16 of hCT(9-32) with either N-methylphenylalanine or d-phenylalanine, respectively. By using confocal laser scanning microscopy we showed that the modifications did neither affect the peptide internalisation efficiency in HeLa nor HEK 293T cells. The metabolic stability of the peptides was investigated in human blood plasma and HEK 293T cell culture supernatant. To analyse the degradation patterns, we used RP-HPLC and MALDI-TOF mass spectrometry. However, we found for all of the new derivatives high metabolic stabilities. In blood plasma, the half-lives for five of the six peptides increased compared to unmodified hCT(9-32). The degradation patterns showed a distinct stabilisation in the N-terminal part of the modified peptides, in the C-terminal part, we found some cleavage to a minor extent. Furthermore, we studied the conformation of the peptides by CD spectroscopy and demonstrated that they possess no cell toxicity. Since our metabolically more stable compounds are still able to pass the cell membrane they provide powerful tools as drug delivery vectors. 相似文献