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301.
302.
J.-O. Karlsson 《Journal of neurochemistry》1980,34(5):1184-1190
Abstract: Rapidly transported fucose-labelled glycoproteins from axons of rabbit retinal ganglion cells were solubilized with nonionic detergents. The labelled glycoproteins carried hydrophobic sites as revealed by a reversible binding to a hydrophobic matrix. A major portion of the labelled glycoproteins carried α-D-mannopyranoside (or glucopyranoside) residues, as shown by a specific interaction with the lectin from Lens culinaris. Three major groups of labelled polypeptides capable of binding to the lectin are described. 相似文献
303.
Incubation of Arthrobotrys dactyloides conidia in the presence of Radopholus citrophilus in lectin solutions with their corresponding sugars did not alter the stimulation of trap formation in solutions containing lectins alone. The lack of inhibition of lectin-stimulated trap formation by sugars or by lectin denaturation and the lack of lectin specificity indicate that the carbohydrate-binding regions of the particular lectins studied are not the stimulatory moieties of these macromolecules. 相似文献
304.
305.
A cultured soybean cell line, SB-1 was used to evaluate the initial interaction between the soybean cells andRhizobium japonicum. Co-culturing ofR. japonicum with SB-1 cells in suspension resulted in strain-specific polar attachment. This attachment can be inhibited by galactose and antibodies raised against seed soybean agglutinin (SBA). A lectin was purified from SB-1 cells which shares properties with SBA in terms of immunological reactivity, sugar binding activity, polypeptide molecular weight and peptide maps. When the SB-1 cells were co-cultured withR. japonicum for three weeks in solid agar medium, histological staining revealed bacterial penetration into certain SB-1 cells. Furthermore, there were focal regions of cells with prominent nuclei representing actively proliferating regions. These observations are analogous to that ofin vivo nodule initiation in soybean roots. 相似文献
306.
柞蚕蛹血淋巴中凝集素的分离鉴定 总被引:2,自引:1,他引:1
在柞蚕蛹血淋巴中可以测得血凝活力,但在大肠杆菌诱导后血淋巴中血凝滴度有很大的升高.本文报道了分离正常柞蚕蛹血淋巴中凝集素的提取步骤.所得的凝集素在SDS垂直板电泳中表现单一条带,免疫扩散呈现单一的沉淀带,分子量为40,000道尔顿.制剂能凝集兔、鸭、豚鼠、羊、马及人的A、B、O和AB型的红细胞.其凝集活力可被半乳糖,乳糖,及N-乙酰半乳糖胺所抑制. 诱导后的柞蚕蛹血淋巴的凝集素成分比较复杂,经亲和层析后的制剂在免疫琼脂双扩散盘中呈现二条沉淀带,在垂直电泳中至少有二条带. 相似文献
307.
The direct double-antibody enzymelinked immunosorbent assay system was used in the detection and measurement of seed lectins from peanut (Arachis hypogaea L.) and soybean (Glycine max L.) plants (PSL and SBL, respectively) that had been inoculated with their respective rhizobia. Concentrations of PSL dropped to undetectable levels in peanut roots at 9 d and stems and leaves at 27 d after planting; SBL could no longer be detected in soybean roots at 9 d and in stems and leaves at 12 d. A lectin antigenically similar to PSL was first detected in root nodules of peanuts at 21 d reaching a maximum of 8 g/g at 29 d then decreasing to 2.5 g/g at 60 d. There was no evidence of a corresponding lectin in soybean nodules.Sugar haemagglutination inhibition tests with neuraminidase-treated human blood cells established that PSL and the peanut nodule lectin were both galactose/lactose-specific. Further tests with rabbit blood cells demonstrated a second mannosespecific lectin in peanut nodule extracts that was not detected in root extracts of four-week-old inoculated plants or six-week-old uninoculated plants, although six-week-old root extracts from inoculated plants showed weak lectin activity. The root extracts from both nodulated and uninoculated plants contained another peanut lectin that agglutinated rabbit but not human blood cells. Haemagglutination by this lectin was, however, not inhibited by simple sugars but a glycoprotein, asialothyroglobulin, was effective in this respect.Abbreviations DAS
double antibody sandwich
- ELISA
enzyme-linked immunosorbent assay
- PBS
phosphate-buffered saline
- PSL
peanut seed lectin
- SBL
soybean lectin 相似文献
308.
用4种不同的植物凝集素制成亲和吸附剂从成熟的稻胚中分离相应内源受体,发现“双丰1号”成熟稻胚中不合与麦胚凝集素(WGA)结合的物质,但含有少量与自身凝集素(S-RGL)和“寒丰”稻胚凝集素(K-RGL)结合的物质(0.1~0.2 mg/g胚),并含有较大量的与伴刀豆球蛋白(conA)结合的物质(1.0~1.5 mg/g胚)。用凝胶电泳分析受体组成,在低pH-不连续PAGE图谱中,conA受体有2条带,S-KGL和H-RGL受体均只有1条迁移率相同的带。SDS-IPAGE图谱显示,conA受体有7条多肽,S-RGL和H-RGL受体具有迁移率相同的8条多肽。说明两种受体的分子性质相同。 相似文献
309.
We have examined the properties and subcellular localization of phytohemagglutinin (PHA), the major lectin of the common bean (Phaseolus vulgaris.), in the axis cells of nearly mature and imbibed mature seeds. On a protein basis the axis contained about 15% as much PHA as the cotyledons. Localization of PHA was done with an indirect immunolabeling method (rabbit antibodies against PHA, followed by colloidal gold particles coated with goat antibodies against rabbit immunoglobulins) on ultra-thin cryosections which were embedded in plastic on the grids after the immunolabeling procedure. The embedding greatly improved the visualization of the subcellular structures. The small (4 nm) collodial gold particles, localized with the electron microscope, were found exclusively over small vacuoles or protein bodies in all the cell types examined (cortical parenchyma cells, vascular-bundle cells, epidermal cells). The matrix of these vacuoles-protein bodies appears considerably less dense than that of the protein bodies in the cotyledons, but the results confirm that in all parts of the embryo PHA is localized in similar structures.Abbreviations IgG
immunoglobulin G
- Mr
relative molecular weight
- PBS
phosphate-buffered saline
- PHA
phytohemagglutinin
- SDS-PAGE
sodium dodecyl sulfate polyacrylamide gel electrophoresis 相似文献
310.
Sodium dodecyl sulfate polyacrylamide gel electrophoresis, immunoblotting, lectin binding, and 125I surface labeling of sporozoites were used to probe sporozoites of the rat coccidian, Eimeria nieschulzi. Analysis of silver stained gels revealed >50 bands. Surface iodination revealed about 14 well labeled, and about 10 weakly labeled but potential, surface proteins. The most heavily labeled surface proteins had molecular masses of 60, 53–54, 45, 28, 23–24, 17, 15, 14, 13, and 12 kD. Following electrophoresis and Western blotting, 2 of the 12 125I labeled lectin probes bound to two bands on the blots, which collectively indicated that two bands were glycosylated. Concanavalin A (ConA) specifically recognized a band at 53 kD, which may represent a surface glycoprotein, and a lectin derived from Osage orange (MPA) bound to a single band at 82–88 kD, that may also be a surface molecule. Immunoblotting using sera collected from rats inoculated orally with oocysts, as well as sera from mice hyperimmunized with sporozoites, revealed that many surface molecules appear to be immunogenic. 相似文献