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91.
Ferguson plots demonstrated that corresponding malate dehydrogenase (MDH) isozymes of Durrant's L and S flax genotrophs differ in apparent molecular weight (MW) and also in net negative charge. The MW differences explain heritable differences in electrophoretic relative mobility (R
m) between corresponding L and S isozymes. The MW for each MDH isozyme was higher for L than for S and resulted in a slowerR
m for L. The net negative charge for each isozyme was higher for L than for S. MDH isozymes also differ in MW within L and S. MW was lower for isozymes in leaves from the bottom of the stem than in leaves from the top of the stem, particularly in L. Integration of information on the MDH isozyme system in the flax genotrophs and information on the peroxidase system suggests the possibility that common modifier loci may controlR
m in both enzymes.The financial assistance of the Natural Sciences and Engineering Research Council of Canada is acknowledged with thanks. 相似文献
92.
The genetics of sympatric Arctic charr [Salvelinus alpinus (L.)] populations from Loch Rannoch, Scotland 总被引:3,自引:0,他引:3
S. E. Hartley C. McGowan † R. B. Greek † A. F. Walker ‡ 《Journal of fish biology》1992,41(6):1021-1031
Two morphologically and ecologically distinct forms of Arctic charr, Salvelinus alpinus , are found in Loch Rannoch, Scotland. The differences in morphology are adaptations to different modes of life, one being pelagic, the other benthic. Both forms have been the subjects of extensive genetic studies including cytogenetics, nuclear and mitochondrial DNA analysis, and protein electrophoresis. Significant differences between the two forms are revealed by some techniques but not others and provide evidence for the reproductive isolation of these two morphs. The findings are discussed in relation to the derivation of sympatry and the phylogenetics of Arctic charr. 相似文献
93.
Photoautotrophic cell supension cultures of C. rubrum exhibit a C3-type of photosynthesis. Yet, up to 20% of total CO2-fixation is directly incorporated into malate and aspartate during short-term photosynthesis. The rate of 14C-labeling of malate and aspartate was doubled if the pH of the medium of the cells was increased from the normal value of 4.5 to 6.0 or 7.0. In vivo 31P-NMR spectroscopy demonstrated that an increase in the external pH from 4.5 to 6.3 increased the cytosolic pH by 0.3 units and the vacuolar pH by about 1.3 units. Possible mechanisms for the effect of extracellular pH on intracellular pH and PEP-carboxylase-dependent carboxylation reactions are discussed. 相似文献
94.
J. L. Starr E. K. Tomaszewski M. Mundo-Ocampo J. G. Baldwin 《Journal of nematology》1996,28(4):565-568
Meloidogyne sp. from five pecan (Carya illinoensis) orchards in Texas were distinctive in host range and iszoyme profiles from common species of Meloidogyne but were morphologically congruent with Meloidogyne partityla Kleynhans, a species previously known only in South Africa. In addition to pecan, species of walnut (Juglans hindsii and J. regia) and hickory (C. ovata) also were hosts. No reproduction was observed on 15 other plant species from nine families, including several common hosts of other Meloidogyne spp. Three esterase phenotypes and two malate dehydrogenase phenotypes of M. partityla were identified by polyacrylamide gel electrophoresis. Each of these isozyme phenotypes was distinct from those of the more common species M. arenaria, M. hapla, M. incognita, and M. javanica. 相似文献
95.
Glycine enhanced the sensitivity of maize phosphenolpyruvate carboxylase to the activator glucose 6-phosphate and reduced the sensitivity of the enzyme to the inhibitors malate and aspartate. The effects of glycine on the kinetic constants for these other effectors were greater than its effect on the Km for substrate, raising the Ki(malate) 11-fold and reducing Ka(glucose6-P) 7-fold, while reducing the Km(PEP) by 3-fold. Kinetically saturating levels of glycine and glucose 6-phosphate acted synergistically to raise Ki(malate) higher than that observed with either activator alone. Glycine and glucose 6-phosphate also synergistically reduced aspartate inhibition. Dual inhibitor analysis indicated that aspartate and malate bind in a mutually exclusive manner, and thus probably compete for the same inhibitor site. In contrast, the synergism between glycine and glucose 6-phosphate indicate that these activators bind at separate sites. Glycine also reduced the Km(Mg) by 3-fold but had no significant effect on the Km of bicarbonate.Abbreviation PEP
phosphoenolpyruvate 相似文献
96.
Although soluble aluminium (Al) has long been recognised as an important limitation to plant growth on acid soils, the biochemical basis of Al toxicity has not been elucidated. Aluminium accumulation in the cell wall may be important, especially the reaction of Al with calcium (Ca) pectate. A study was conducted to investigate the effects of six ligands, citrate, malate, galacturonate, fluoride, sulfate and chloride, on the sorption of Al by Ca pectate prepared from two sources of pectin that differed in degree of methyl esterification (DE). The sorption of Al by Ca pectate increased linearly with increase in Al added from 25 to 100 µM (or 50 to 200 µM in the case of Al2(SO4)3). There was a significant reduction in Al sorption in the presence of those ligands that form strong complexes with Al, especially citrate and, to a lesser extent, malate and fluoride. There was little difference in Al sorption by Ca pectate prepared from pectin of differing DE. Calcium in the supernatant solution increased linearly by 1.5 nmol for each 1 nmol increase in Al sorbed. The results support the hypothesis that strong complexes of Al with organic and inorganic ligands reduce Al sorption by Ca pectate in the cell wall. 相似文献
97.
Mahmood Vessal Seyed Mohammad Bagher Tabei 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》1996,113(4):757-763
Cytoplasmic malate dehydrogenase from ovine liver Echinococcus granulosus protoscolices was purified 22-fold by QAE- and SP-Sephadex chromatography. The pH optimum of the enzyme was 8.0 in either Tris-HCl or barbital buffer. The κm values of oxaloacetate and NADH were 0.400 ± 0.018 and 0.410 ± 0.038 mM, respectively. The enzyme lost about 90% of its activity when heated for 2 min at 65°C. A 61.4% inhibition of the enzyme was noted at 4 mM concentration of diethyl pyrocarbonate. A 3 mM concentration of fructose 1,6-diphosphate inhibited the enzyme by 76.5%. The inhibition was non-competitive with respect to NADH with a κi value of 0.85 mM. A 75% inhibition of the enzyme was noted at 1 mM concentration of mebendazole that inhibited the enzyme upon competing with NADH with a κi value of 0.176 mM. A 2-mM concentration of citrate almost doubled the enzyme activity. The enzyme was inhibited at high concentrations of either substrate. The enzyme was not inhibited by p-hydroxymercuribenzoate or fumarate. The enzyme was absolutely specific for NADH as a cofactor. The properties of this enzyme are compared with those of the enzyme from the host liver, the cyst fluid and some other animal sources. The results are discussed in terms of the differences among the properties of the host liver, the cyst fluid and the protoscolices enzymes. The biochemical basis for the use of mebendazole in the treatment of echinococcosis is also elucidated. 相似文献
98.
Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) EC 1.1.1.40, malic enzyme, has been purified 40-fold to a homogeneous state using affinity chromatography and gel permeation chromatography. The Mr is 260–265 K with four subunits each of 64–65 K. The enzyme has some competitive or non-competitive inhibitors, particularly some of the Krebs cycle acids and exhibits a rapid rise in activity at the same time as activity of the enzymes of the Krebs cycle are decreasing in the tomato mitochrondrion. The malic enzyme is restricted to the cytosol. The relevance of this information to malate metabolism in plants is discussed. 相似文献
99.
J. H. Adams J. H. Koeslag 《European journal of applied physiology and occupational physiology》1989,59(3):189-194
Post-exercise ketosis is known to be suppressed by physical training and by a high carbohydrate diet. As a result it has often been presumed, but not proven, that the development of post-exercise ketosis is closely related to the glycogen content of the liver. We therefore studied the effect of 1 h of treadmill running on the blood 3-hydroxybutyrate and liver and muscle glycogen concentrations of carbohydrate-loaded trained (n = 72) and untrained rats (n = 72). Resting liver and muscle glycogen levels were 25%-30% higher in the trained than in the untrained animals. The resting 3-hydroxybutyrate concentrations of both groups of rats were very low: less than 0.08 mmol.l-1. Exercise did not significantly influence the blood 3-hydroxybutyrate concentrations of trained rats, but caused a marked post-exercise ketosis (1.40 +/- 0.40 mmol.l-1 h after exercise) in the untrained animals, the time-course of which was the approximate inverse of the changes in liver glycogen concentration. Interpreting the results in the light of similar data obtained after a normal and low carbohydrate diet it has been concluded that trained animals probably owe their relative resistance to post-exercise ketosis to their higher liver glycogen concentrations as well as to greater peripheral stores of mobilizable carbohydrate. 相似文献
100.