排序方式: 共有78条查询结果,搜索用时 15 毫秒
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研究了斜生褐孔菌多糖对人类肝癌HepG-2细胞凋亡的诱导作用。采用噻唑蓝法(MTT法)观察了斜生褐孔菌多糖对HepG-2细胞生长的影响,用透射电镜观察了细胞形态,用DNA Ladder检测了细胞凋亡,用流式细胞仪检测了细胞凋亡率;同时采用逆转录-聚合酶链反应法(RT-PCR法)研究了不同浓度斜生褐孔菌多糖作用后HepG-2细胞中Bax和Bcl-2基因mRNA转录水平的变化。结果表明斜生褐孔菌多糖能抑制HepG-2细胞增殖,并呈时间剂量依赖关系;电镜观察、DNALadder和流式细胞仪检测均证实了斜生褐孔菌多糖能够诱导HepG-2细胞凋亡;经斜生褐孔菌多糖处理后,HepG-2细胞中Bax基因mRNA转录水平增强,而Bcl-2基因mRNA无明显变化。证明了斜生褐孔菌多糖具有抑制HepG-2细胞生长及诱导HepG-2细胞凋亡的作用,这可能与调节Bcl-2和Bax基因表达水平有关。 相似文献
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Heidy Schimann Jason Vleminckx Christopher Baraloto Julien Engel Gaelle Jaouen Eliane Louisanna Sophie Manzi Audrey Sagne Mélanie Roy 《Biotropica》2020,52(3):444-456
The influence exerted by tree communities, topography, and soil chemistry on the assembly of macrofungal communities remains poorly understood, especially in highly diverse tropical forests. Here, we used a large dataset that combines inventories of macrofungal Basidiomycetes fruiting bodies, tree species composition, and measurements for 16 soil physicochemical parameters, collected in 34 plots located in four sites of lowland rain forests in French Guiana. Plots were established on three different topographical conditions: hilltop, slope, and seasonally flooded soils. We found hyperdiverse Basidiomycetes communities, mainly comprising members of Agaricales and Polyporales. Phosphorus, clay contents, and base saturation in soils strongly varied across plots and shaped the richness and composition of tree communities. The latter composition explained 23% of the variation in the composition of macrofungal communities, probably through high heterogeneity of the litter chemistry and selective effects of biotic interactions. The high local heterogeneity of habitats influenced the distribution of both macrofungi and trees, as a result of diversed local soil hydromorphic conditions associated with contrasting soil chemistry. This first regional study across habitats of French Guiana forests revealed new niches for macrofungi, such as ectomycorrhizal ones, and illustrates how macrofungi inventories are still paramount to can be to understand the processes at work in the tropics. Abstract in Spanish is available with online material. 相似文献
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基于流式细胞技术的灵芝基因组大小估测 总被引:2,自引:0,他引:2
以药典规定的灵芝正品来源灵芝Ganoderma lucidum作为研究对象,利用已完成全基因组测序的黑曲霉Aspergillus niger作为内标,通过机械破碎菌丝体的方法获得合适浓度的细胞核悬液,碘化丙啶荧光染色后成功应用流式细胞术进行基因组大小估测。经过优化材料培养、样品制备、上机分析等实验条件,估测得出灵芝基因组大小(48.98±0.60)Mb,为灵芝基因组学研究提供重要数据。该方法简捷稳定,在蕈菌范围内,首次得到了全基因组测序数据与光学图谱结果验证,为蕈菌基因组学研究提供重要技术平台。 相似文献
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安徽琅琊山大型真菌区系多样性 总被引:4,自引:1,他引:3
对安徽省琅琊山自然保护区大型真菌区系多样性进行了分析.结果表明该地区共有大型真菌107种,分属于25科56属,其中优势科为多孔菌科Polyporaceae(20种,占18.69%)、口蘑科Tricholomataceae(14种,占13.08%)、丝膜菌科Cortinariaceae(11种,占10.28%)、红菇科Russulaceae(10种,占9.35%),以上4科仅占琅琊山大型真菌总科数的16%,而种数占全部种数的51.4%;优势属有鹅膏属Amanita(8种,占7.48%)、栓菌属Trametes(7种,占6.54%)、乳菇属Lactarius(5种,占4.67%)、小皮伞属Marasmius(5种,占4.67%)、红菇属Russula(5种,占4.67%),共包括30种,占全部种数的28.04%.从属的区系地理成分上可分为:世界性分布成分(66.07%)、泛热带分布成分(19.64%)、北温带分布成分(10.71%)、东亚分布成分(1.79%).琅琊山大型真菌主要是以世界广布成分为主,其次是泛热带成分和北温带成分,这种分布状况可能与该地区的气候、地理和植被等自然条件的特征有着密切的关系.从种的组成上可划分为世界性分布种(70.09%)、温带分布种(14.95%)、热带-亚热带分布种(14.02%)及中国-日本共有种(0.93%)等4个分布类型,表现出一定的热带向温带过渡的区系特征. 相似文献
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Eef Arnolds 《Plant biosystems》2013,147(6):779-795
Abstract The motives for the conservation of fungi are briefly outlined. The data and methods needed for successful use of fungi in environmental conservation are mentioned. A survey is given of the main habitats of threatened fungi in Western and Central Europe, including data on the significance of their mycoflora, the causes of decrease and possible measures to improve the situation. The value of macrofungi as bio-indicators for environmental quality is discussed and demonstrated with three examples: the indicator value of wood-inhabiting fungi for undisturbed forest sites; of ectomycorrhizal fungi for air pollution stress in forests and of saprotrophic fungi for the duration of undisturbed grassland use. Lists of indicator species with different indicator values for air pollution stress and grassland use are provided. 相似文献
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Phylogenetic diversity of macromycetes and woody plants along an elevational gradient in Eastern Mexico
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Marko Gómez‐Hernández Guadalupe Williams‐Linera Deborah J. Lodge Roger Guevara Eduardo Ruiz‐Sanchez Etelvina Gándara 《Biotropica》2016,48(5):577-585
Phylogenetic information provides insight into the ecological and evolutionary processes that organize species assemblages. We compared patterns of phylogenetic diversity among macromycete and woody plant communities along a steep elevational gradient in eastern Mexico to better understand the evolutionary processes that structure their communities. Macrofungi and trees were counted and identified in eight sites from 100 to 3500 m asl, and sequence data retrieved from GenBank for the same or closely related species were used to reconstruct their phylogenies. Patterns of species richness and phylogenetic diversity were similar for both macrofungi and trees, but macromycete richness and diversity peaked at mid‐elevations, whereas woody plant richness and diversity did not show significant trends with elevation. Phylogenetic similarity among sites was low for both groups and decreased as elevational distance between sites increased. Macromycete communities displayed phylogenetic overdispersion at low elevations and phylogenetic clustering at high elevations; the latter is consistent with environmental filtering at high elevation sites. Woody plants generally exhibited phylogenetic clustering, consistent with the potential importance of environmental filtering throughout the elevational gradient. 相似文献