首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   1772篇
  免费   322篇
  国内免费   418篇
  2024年   19篇
  2023年   60篇
  2022年   51篇
  2021年   68篇
  2020年   80篇
  2019年   103篇
  2018年   108篇
  2017年   117篇
  2016年   97篇
  2015年   87篇
  2014年   96篇
  2013年   95篇
  2012年   74篇
  2011年   91篇
  2010年   82篇
  2009年   99篇
  2008年   114篇
  2007年   93篇
  2006年   100篇
  2005年   82篇
  2004年   80篇
  2003年   91篇
  2002年   58篇
  2001年   56篇
  2000年   51篇
  1999年   40篇
  1998年   38篇
  1997年   25篇
  1996年   34篇
  1995年   26篇
  1994年   29篇
  1993年   36篇
  1992年   30篇
  1991年   24篇
  1990年   28篇
  1989年   21篇
  1988年   12篇
  1987年   10篇
  1986年   12篇
  1985年   22篇
  1984年   9篇
  1983年   8篇
  1982年   9篇
  1981年   10篇
  1980年   12篇
  1979年   10篇
  1978年   5篇
  1977年   4篇
  1976年   2篇
  1958年   4篇
排序方式: 共有2512条查询结果,搜索用时 31 毫秒
61.
黄土丘陵半干旱区不同作物生态效能研究   总被引:5,自引:0,他引:5  
深入研究特定生态环境条件下不同作物生产力及其生态效能,对于合理调控农田生态系统,探讨提高系统生产力途径具有重要意义。60年代以来,在国际生物学计划(IBP)的影响下,美国、日本、菲葎宾等国先后进行了小麦、水稻、大豆等作物农田生态效能研究。我国于“六·五”期间分别在南方稻区、黄淮海平原以及北方灌区等不同农业区开展了多种作物种植制度生产力问题研究,并取得重  相似文献   
62.
Primary productivity has been measured routinely at Lake Tahoe since 1967, and a number of mechanisms underlying variability in the productivity record have now been identified. A long-term trend due to nutrient loading dominates the series. Seasonality also is prominent, apparently controlled by direct physical factors unrelated to the trophic cascade. A 3-yr cycle has been detected and several possible mechanisms are considered. Irregular fluctuations also are present, caused in part by isolated events (a forest fire) and recurring but variable phenomena (spring mixing). Except possibly for the 3-yr cycle, the known sources of variability appear to operate bottom-up through direct physical and chemical effects on the phytoplankton.  相似文献   
63.
64.
The profiles of photon flux density incidented on a tubularloop photobioreactor in the day could be altered by inclining the bioreactor at an angle with the horizontal. The photon flux density at noon decreased with increasing angle of inclination, whereas the photon flux density in the early morning and late afternoon increased with increasing angle of inclination. The overall photosynthetic radiance received by the bioreactor inclined at 0, 25, 45, and 80 degrees was 1:0.89:0.77:0.62. Regardless of the angle of bioreactor inclination, the overall biomass output rate of a fed-batch culture over an 8-h/day period was comparable (26-36 g-biomass m(-2) bioreactor surface area day(-1)). As a bioreactor inclined at an angle occupied smaller land area, and daily biomass output rate per land area of a bioreactor inclined at 80 degrees (130 g-biomass m(-2) land) was about six times of that obtainable at horizontal position (21-g biomass m(-2) land). The bioenergetics growth yield from the absorbed photosynthetic radiance was not a constant but an inverse function of the photon flux density. The quasi-steady state chlorophyll content of the Chlorella cells varied between 36 and 63 mg g(-1) cells. Photoinhibition of the maximum photosynthetic capacity was not observed in this study.  相似文献   
65.
An expression vector for G-CSF, pASLB3-3, was constructed and introduced into Namalwa KJM-1 cells (Hosoi et al., 1988), and cells resistant to 100 nM of methotrexate (MTX) were obtained. Among them, the highest producer, clone SC57, was selected and the productivity of this clone was further characterized. The maximal production of G-CSF was at the most 1.8 g/ml/day using a 25 cm2 tissue culture flask, even though the cell number was above 7×105 cells/ml. The limiting factors at high density were analyzed as the deficiency of nutrients, such as glucose, cysteine and serine, and pH control. The depression of specific G-CSF productivity per cell under the batch culture conditions was overcome by using a perfusion culture system, BiofermenterTM (Sato, 1983) with modifications of nutrients supplementation by a dialysis membrane and/or dissolved oxygen (DO) supplementation by microsilicone fibers. ITPSGF medium was modified to elevate concentrations of amino acids and glucose by 2.0- and 2.5-times, respectively. Under the control of pH at 7.4 and DO at 3 ppm, the specific G-CSF productivity was not depressed even at high cell density (above 1×107 cells/ml), and the amount of G-CSF reached 41 g/ml. These results indicated the possibility of finding the optimum culture conditions for the production of recombinant proteins by Namalwa KJM-1 cells.Abbreviations ABTS 2,2-Azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid - BSA Bovine Serum Albumin - BSA-PBS Phosphate-buffered Saline without Ca2+ and Mg2+ containing Bovine Serum Albumin - dhfr Dihydrofolate Reductase - DO Dissolved Oxygen - G-CSF Granulocyte Colony-stimulating Factor - HEPES 4-(2-Hydroxyethyl)-1-piperazineethansulfonic Acid - IFN Interferon - MTX Methotrexate - PBS(-) Phosphate-buffered saline without Ca2+ and Mg2+ - Tween-PBS Phosphate-buffered saline without Ca2+ and Mg2+ containing 0.05% of Tween 20  相似文献   
66.
Water quality and plankton periodicity was studied in two mine lakes near Jos in the younger granite area of Plateau State, Nigeria. The investigation was carried out for 8 months. Transparency, pH and NO3-N were significantly higher in Lake II while DOM, alkalinity, PO 4 3– -P, BOD and chloride were significantly higher in Lake I. The order of dominance in Lake I was Bacillariophyceae, Cyanophyceae, Chlorophyceae and Dinophyceae while for Lake II; Chlorophyceae, Cyanophyceae, Bacillariophyceae and Dinophyceae. In Lake I the zooplankton in order of dominance were Cladocera, Rotifera, Copepoda, and nauplii and in Lake II Cladocera, Copepoda, Rotifera and nauplii. Although both lakes would seem unproductive based on the PO 4 3– -P and NO 3 -N levels, Lake I appears more productive than Lake II.  相似文献   
67.
Comparative limnology of Sambhar and Didwana lakes (Rajasthan,NW India)   总被引:1,自引:1,他引:0  
Two alkaline saline inland lakes of Indian arid region were studied during 1984 and 1985, to assess functioning and interaction of various environmental and biological factors. Changes in physical and chemical variables, planktonic composition, chlorophyll content and phytoplankton primary productivity were examined.Salinity in both lakes fluctuated from almost fresh water (1.80), to hypersaline (300) and acted as the main controlling factor for almost all the biotic parameters. Maximum total alkalinities were 2162 mg l–1 and 2090 mg l–1, respectively in Sambhar and Didwana lakes. Dissolved oxygen ranged from completely anoxic conditions to maxima of 11.68 and 7.29 mg 1–1, respectively in Sambhar and Didwana lakes. Nutrient enrichment in the lakes was low.The phytoplankton species composition of Sambhar lake was reduced from an earlier reported 20 genera to only 11 (Nostoc, Microcystis, Spirulina, Aphanocapsa, Oscillatoria, Merismopedia, Nitzschia, Navicula, Synedra, Cosmarium and Closterium). Phytoplankton of Didwana was composed of only 9 genera including Anabaena and Nodularia. Sambhar lake, which once contained Artemia, is now totally devoid of them. On the other hand, Artemia was the most dominant zooplankter in Didwana lake at a salinity range of 15–288. Other zooplankters such as Moina, Cyclops and Brachionus flourished at lower salinity levels in Didwana lake. The seasonal quantitative and qualitative phyto- and zooplankton changes in relation to salinity are documented.  相似文献   
68.
Annual net production was estimated in the secondary coppice forest near Tokyo, which was dominated by a deciduous oak,Quercus serrata Thunb. Lateral growth of stems and old branches was directly estimated by examining the annual rings for 35 shoots in a clear-cut quadrat of 10m×10m. Phytomasses of current organs were also weighed in the quadrat. Preharvest losses of current organs were determined by twelve 0.5 m2 litter traps for fine litter and twelve 6 m2 quadrats for woody litter. Branch production was also assessed indirectly by use of the stem-branch allometry and death of branches. The results of the indirect method were in sufficient agreement with the result of the direct one. Grazing loss of leaves from the canopy was estimated directly from the loss in leaf area and indirectly from the animal faeces caught by the litter traps. Net production of the canopy trees was 149 kg a−1 year−1, in which leaf production was 36.9 kg. Animals grazed about 14% of the leaf area by the end of the growing season. True consumption of leaves by animals was 7.6% of leaf production or 10% of leaf mass. Production of undergrowth, mainly a dwarf bamboo,Pleioblastus chino Makino, was 28 kg a−1 year−1, being 15% of the total stand production. Productivity of this forest was significantly higher than that of cool-temperate deciduous broadleaf forests.  相似文献   
69.
A “planted core” system was developed to test the effect of short term (1–2 weeks) experimental manipulation of environmental parameters on edaphic microalgae under field conditions. A large number of small cores (surface area = 7 cm2) were collected, randomized and replanted in the marsh in fiddler crab exclosures with appropriate experimental treatments. Daily enrichment of the cores with NH4+ resulted in significant increases in edaphic primary productivity and levels of chlorophyll a in both summer and winter seasons in the short-Spartina marsh. Enrichment with a complete nutrient solution caused no further increases. Nutrient enrichment of creekbank sediments was much less stimulatory to the resident algal assemblage. In both sites, but especially in the creekbank, the removal of fiddler crab grazers resulted in significant increases in chlorophyll a and productivity. Experimental manipulation of light intensity showed that the average light intensity reaching the sediment surface was saturating for chlorophyll production in the short-Spartina marsh. A reciprocal transplant experiment involving unfertilized cores from the short-Spartina marsh and creekbank marsh demonstrated that NH4+ inputs occurring in the creekbank site rapidly alleviated nitrogen limitation of edaphic algae from short-Spartina marsh. Algae in creekbank cores incubated in the short-Spartina marsh were unable to sustain high productivity once the original standing stock of NH4+ declined.  相似文献   
70.
Summary Differences in the productivity of establishedP. radiata plantations on pasture and forest soils were found to be reproducible withP. radiata seedlings in a glasshouse environment. The growth of seedlings on pasture soil exceeded that of seedlings on native forest soil (the ‘primary pasture effect’). After a history of pine on both native and pasture soils a residual effect of pasture on seedling growth was evident (the ‘secondary pasture effect’). However, the effect of a history of pine plantation (the ‘pine effect’) was to decrease the productivity of both native and pasture soil as assessed by seedling growth. These effects were not related to changes due to land management in mycorrhizal infection or in soil structure. The analysis of seedling growth leads to the conclusion that soil fertility, particularly the availability of nitrogen and phosphorus, has changed.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号