New technique in optical resolutions

The present paper illustrates the development of an advanced technique in optical resolution. Both of the amphetamine enantiomers can be obtained by a two-step distillation in nearly quantitative yield without any loss of the resolving agent. It is proved that the second-order interactions (H-bond) are sufficient for separation of enantiomers by distillation. © 1994 Wiley-Liss, Inc.     

热带林茎流收集及计算方法探索

热带林茎流收集及计算方法探索周光益,吴仲民,李意德,陈步峰（中国林科院热带林业研究所,广州５１０５２０）ＣｏｌｌｅｃｔｉｏｎａｎｄＣａｌｃｕｌａｔｉｏｎＭｅｔｈｏｄｓｆｏｒＳｔｅｍｆｌｏｗｉｎＴｒｏｐｉｃａｌＦｏｒｅｓｔ．￥ＺｈｏｕＧｕａｎｇｙｉ;Ｗ...     

Analytical study of the effects of some soil and plant parameters on root growth due to absorption of one mobile ion: A free-boundary model

The object of our study is: a model for root growth through a free-boundary problem and the effects resulting from differences in nutrient availability and transport of only one mobile nutrient between the root surface and the rhizosphere produced by an absorption Michaelis-Menten for low and high concentrations. The model equations are solved by two methods: the quasi-stationary method and the balance integral method. The numerical solutions are used to compute radial root growth. Curves of nutrient concentration at the root-soil interface, curve as a function of root radius as well as curves representing root radius as a function of time are plotted. The parameters which are varied are the root absorption power, flux velocity at the root surface, efflux, rhizosphere radius, diffusion coefficient, buffer power, and maximum influx. The two methods show the theoretical results for radial root growth in the range of low and high concentrations. The balance integral method provides more detailed information.     

Bayesian analysis of linkage between genetic markers and quantitative trait loci. I. Prior knowledge

Summary Prior information on gene effects at individual quantitative trait loci (QTL) and on recombination rates between marker loci and QTL is derived. The prior distribution of QTL gene effects is assumed to be exponential with major effects less likely than minor ones. The prior probability of linkage between a marker and another single locus is a function of the number and length of chromosomes, and of the map function relating recombination rate to genetic distance among loci. The prior probability of linkage between a marker locus and a quantitative trait depends additionally on the number of detectable QTL, which may be determined from total additive genetic variance and minimum detectable QTL effect. The use of this prior information should improve linkage tests and estimates of QTL effects.     

Extra-weak chemiluminescence of drugs XV. Method for determining stability of Toki-shakuyaku-san extract granules

The purpose of the study was to evaluate the quality of Toki-shakuyaku-san extract granules (TJ-23) using chemiluminescence (CL). A linear relationship was obtained between the log value of the CL of TJ-23 and the reaction temperature. An excellent correlation (r= 0.999) was found between the slope of this curve (ΔA) and the colour intensity due to the browning reaction occurring at the early stage of the Maillard reaction.     

The enhancement of iron-dependent luminol peroxidation by 2,2′-Dipyridyl and nitrilotriacetate

The generation of radicals from luminol and H₂O₂, in the presence of iron and iron chelates was monitored by measuring the chemiluminescence produced by further oxidation of these radicals. 2,2′-Dipyridyl enhanced the production of chemiluminescence in the presence of FeSO₄, farritin and haemosiderin but not FeCI₃ or horseance of both FeSO₄ and FeCI₃ but not ferritin or haemosiderin. The enhancement of chemiluminescence by iron chelation may have analytical applications and the process by which these iron chelates are able to generate radicals from the nitrogenous base luminol may be similar to that responsible for their toxic effects on DNA.     

Effect of oxygen abstraction on the peroxidase–luminol–perborate system: Relevance to the HRP enhanced chemiluminescence mechanism

Abstraction of oxygen from the HRP enhanced chemiluminescence system has no significant effect on the chemiluminescence generated. It is, therefore, proposed that in the peroxidase-luminol-perborate system at pH 7.3, chemiluminescence is generated by a direct reaction of diazaquinones with hydrogen peroxide and not, as generally assumed, from the reaction of luminol radicals with the molecular oxygen.     

Characterization of peroxidases in luminol chemiluminescence coupled with copper-catalysed oxidation of cysteamine

Hydrogen peroxide formed during the course of the copper(II)-catalysed oxidation of cysteamine with oxygen was continuously determined by a peroxidase (POD)-catalysed luminol chemiluminescence (CL) method. Horseradish peroxidase (HRP), lactoperoxidase (LPO) and Arthromyces ramosus peroxidase (ARP) were used as a CL catalyst. The respective PODs gave specific CL intensity-time profiles. HRP caused a CL delay, and ARP gave a time-response curve which followed the production rate of H₂O₂. LPO gave only a weak CL flash which decayed promptly. These differences of CL response curves could be explained in terms of the different reactivities of PODs for superoxide anion and the different formation rate of luminol radicals in the peroxidation of luminol catalysed by POD.     

天花粉蛋白与FMP复合物的晶体结构

用浸泡法得到了天花粉蛋白（ＴＣＳ）与ＦＭＰ复合物的晶体,在ＳＩＭＥＮＮＳＸ－２００Ｂ面探测器系统上收集了一套２．０分辨率的Ｘ射线衍射数据。用同晶差值傅立叶法解析了复合物的结构,经Ｘ—ＰＬＯＲ程序修正得到了ＴＣＳ—ＦＭＰ复合物的分子结构并找出了１９７个水分子,最后的Ｒ因子为０．１７２,键长和键角的ＲＭＳ偏差分别为０．０１５和２．９２２度。ＴＣＳ—ＦＭＰ复合物中,ＦＭＰ与天花粉蛋白分子有较好的结合,其结合位置正处于根据三维结构和突变体信息推测的Ｎ一糖苷酶活性口袋之中。它的类嘌呤环夹在Ｙ７０和Ｙ１１１两个侧链环之间,与Ｙ７０环近乎平行,其Ｎ７和Ｎ６分别与ＴＣＳ分子的Ｇ１０９４羰基氧和Ｉ７１的Ｎ成氢键,Ｎ３靠近Ｒ１６３的侧链,其磷酸根则伸向活性口袋的底部,与Ｅ１８９、Ｅ１６０和Ｒ１６３等残基作用。     

工业固体废弃物的急性生物毒性研究

工业固体废弃物的急性生物毒性研究谢思琴,顾宗濂,周德智（中国科学院南京土壤研究所,南京２１０００８）Ａｃｕｔｅｂｉｏｔｏｘｉｃｉｔｙｏｆｉｎｄｕｓｔｒｉａｌｓｏｌｉｄｗａｓｔｅｓ￥．ＸｉｅＳｉｑｉｎ;ＧｕＺｏｎｇｌｉａｎａｎｄＺｈｏｕＤｅｚｈｉ（Ｉｎ...