全文获取类型
收费全文 | 3904篇 |
免费 | 206篇 |
国内免费 | 164篇 |
出版年
2024年 | 5篇 |
2023年 | 49篇 |
2022年 | 98篇 |
2021年 | 89篇 |
2020年 | 97篇 |
2019年 | 139篇 |
2018年 | 136篇 |
2017年 | 96篇 |
2016年 | 91篇 |
2015年 | 106篇 |
2014年 | 217篇 |
2013年 | 314篇 |
2012年 | 140篇 |
2011年 | 265篇 |
2010年 | 192篇 |
2009年 | 189篇 |
2008年 | 172篇 |
2007年 | 176篇 |
2006年 | 171篇 |
2005年 | 172篇 |
2004年 | 118篇 |
2003年 | 96篇 |
2002年 | 71篇 |
2001年 | 48篇 |
2000年 | 28篇 |
1999年 | 37篇 |
1998年 | 47篇 |
1997年 | 48篇 |
1996年 | 39篇 |
1995年 | 38篇 |
1994年 | 26篇 |
1993年 | 41篇 |
1992年 | 44篇 |
1991年 | 23篇 |
1990年 | 24篇 |
1989年 | 28篇 |
1988年 | 18篇 |
1987年 | 14篇 |
1986年 | 17篇 |
1985年 | 53篇 |
1984年 | 141篇 |
1983年 | 87篇 |
1982年 | 93篇 |
1981年 | 55篇 |
1980年 | 41篇 |
1979年 | 32篇 |
1978年 | 22篇 |
1977年 | 8篇 |
1975年 | 9篇 |
1974年 | 7篇 |
排序方式: 共有4274条查询结果,搜索用时 31 毫秒
101.
To facilitate structural studies of glycoproteins, reverse-phase high-pressure liquid chromatography (HPLC) methods have been developed for preparative isolation of glycopeptides and have been applied to human ceruloplasmin as an example of glycopeptides containing glucosamine (GlcN) and to human immunoglobulin D (IgD) for glycopeptides containing galactosamine (GalN). The use of RP-P columns and of trifluoroacetic acid and heptafluorobutyric acid as counterions was investigated. Various elution systems (both isocratic and programmed gradient) were used with n-propanol to assess the relative hydrophilicity of the peptides. The procedure developed for the GlcN glycopeptides of ceruloplasmin enabled purification of nine major chymotryptic peptides (ranging in size from 15 to 29 residues) and also of many minor peaks. These were characterized by amino acid and endgroup analysis, and the complete sequence of five was determined. These represent three different sites of GlcN attachment in the amino-terminal half of the ceruloplasmin chain. The procedures developed have enabled isolation of glycopeptides from ceruloplasmin having a single GlcN oligosaccharide attached; the latter are valuable for study of the structure and function of the carbohydrate groups. Separation of GalN glycopeptides from IgD was more difficult because of the high content of GalN in the hinge. Purification and sequence analysis was aided by partial removal of sugar by treatment with HF and by other methods. Four (or five) GalN oligosaccharides are attached to serine or threonine residues in the IgD hinge region, and all but one are in close proximity in the repeating sequence Ala-Thr-Thr-Ala-Pro-Ala-Thr-Thr. 相似文献
102.
Determination of proton dissociation constants by ion-exchange high-performance liquid chromatography 总被引:1,自引:0,他引:1
R J De Wit 《Analytical biochemistry》1982,123(2):285-290
The common methods to determine dissociation constants of solutes, e.g., uv spectrophotometry, potentiometry, and conductimetry, are accurate but require at least 1 nmol of compound. High-performance liquid chromatography (HPLC) allows 1 pmol of a uv-absorbing compound to be detected. By adjusting the polarity of the mobile phase, reverse and normalphase properties of an ion-exchanger can be minimized, resulting in a high correlation between charge and retardation of the solute. Thus, the degree of ionization of several compounds was monitored in mobile-phase compositions of different pH values using cation exchange. The pK values of several pterin derivatives corresponded to those obtained by other methods. In addition, pK values of two unidentified pterin derivatives were determined, using only 20 pmol of each. 相似文献
103.
Jan T. Keltjens William B. Whitman C. Gerda Caerteling Annette M. van Kooten Ralph S. Wolfe Godfried D. Vogels 《Biochemical and biophysical research communications》1982,108(2):495-503
2-Mercaptoethanesulfonic acid (coenzyme M), or a derivative of it, and a yellow chromophore, known as the nickel-containing tetrapyrrole factor F430, occur in the prosthetic group of methylcoenzyme M reductase in an equimolar amount, and bound to each other; this enzyme catalyzes the final step of methane production. The prosthetic group, which is called coenzyme MF430, was isolated from the purified enzyme and was extracted from cells. The presence of coenzyme M was confirmed by a bioassay using Methanobrevibacter ruminantium and by the use of chemical and physicochemical analyses. 相似文献
104.
Hugo J. Niggli Peter A. Cerutti 《Biochemical and biophysical research communications》1982,105(3):1215-1223
The nucleosomal distribution of cis-syn cyclobutyl-type thymine photodimers was determined in normal human skin fibroblasts following irradiation with low doses of far-ultraviolet light at 254 nm and nearultraviolet light at 313 nm. The thymine photodimer concentrations were determined by high pressure liquid chromatography in acid hydrolysates of total cellular DNA and of nucleosomal core- and chromatosomal-DNA. The lesion concentrations in linker-DNA were calculated from these data. While thymine photodimers were distributed uniformely following 254 nm irradiation they were enriched by a factor of 2.4 – 4.2 in nucleosomal linker DNA after exposure to 313 nm light. 相似文献
105.
Pei-Lu Chiu Peter P. Fu Shen K. Yang 《Biochemical and biophysical research communications》1982,106(4):1405-1411
-3,4-, 5,6-, 8,9-, and 10,11-dihydrodiols formed from the metabolism of 7-fluorobenz[a]anthracene by rat liver microsomes were isolated by reversed-phase high performance liquid chromatography. Ultraviolet absorption, mass, and NMR spectral analyses indicated that the 5,6- and 8,9-dihydrodiols were preferentially in quasi-diaxial conformations, whereas the 3,4- and 10,11-dihydrodiols were preferentially in quasi-diequatorial conformations. CPK space-filling models suggest that the quasi-diaxial conformation is primarily the result of electronic repulsion between the fluorine and the hydroxyl oxygen. These findings provide a structural basis in the interpretation of the carcinogenic potencies of some fluorinated polycyclic aromatic hydrocarbons. 相似文献
106.
Metabolism and activation of benzo[a]pyrene by mouse and rat skin in short-term organ culture and in vivo 总被引:1,自引:0,他引:1
The metabolic activation of BP was examined in mouse and rat skin in vivo and in short-term organ culture. In mouse skin, larger quantities of ether- and water-soluble metabolites were formed and more BP became bound covalently to DNA and protein than in rat skin. Qualitative differences in the formation of dihydrodiol metabolites and of BP-deoxyribonucleoside adducts between mouse and rat skin were also observed. Organ culture techniques may not provide a true model of metabolic activation in vivo because it was found that the covalent binding of BP to DNA and protein was reduced in skin maintained in culture despite an accumulation of dihydrodiol and other ether-soluble metabolites. In addition, the proportions of the syn- and anti-isomers of BP-7,8-diol 9,10-oxide involved in the formation of adducts with deoxyguanosine differed between skin treated in organ culture and in vivo. 相似文献
107.
A new method was developed to study the unsupplemented tryptophan hydroxylase system in brain tissue slices from the raphe nuclei of the rat by high-performance liquid chromatography (HPLC) with fluorescence detection. Tryptophan hydroxylase activity was measured by determining 5-hydroxytryptophan (5-HTP) accumulation in raphe nuclei slices containing all of the enzyme system (the hydroxylase, tetrahydrobiopterin, and dihydropteridine reductase) in the presence of NSD-1055 (an inhibitor of aromatic l-amino acid decarboxylase). An optimum temperature was observed at 25°C and the reaction progressed linearly for 60 min. The hydroxylation of tryptophan was maximal by the addition of 0.2 mM tryptophan in the medium. A maximum 1.5-fold activation was shown at 0.2 mM 6-methyltetrahydropterin in the presence of 10 mM dithiothreitol. Dithiothreitol alone did not affect the activity. A 1.5-fold activation was observed when incubation was carried out under gas phase of 95% oxygen and 5% CO2 instead of air. The activity was inhibited by 75% at 10?4 M p-chlorophenylalanine. Both A-23187, a calcium ionophore, and dibutyryl cyclic AMP (DBc-AMP) stimulated the hydroxylation of tryptophan. The activation by A-23187 plus DBc-AMP was more than additive, suggesting the two activating mechanisms by Ca2+ and cyclic AMP may be operating synergistically. 相似文献
108.
J.Peter H. Burbach Xinchang Wang Marjanne van Ittersum 《Biochemical and biophysical research communications》1982,108(3):1165-1171
Arginine-vasopressin and oxytocin, peptides which serve as putative precursors for neurotrophic fragments, were digested in the presence of the respective 14C-Tyr2- and nonapeptides with a purified synaptic membrane preparation of rat brain. In this preparation aminopeptidase activity predominates in the conversion of these peptides. The disappearance of intact peptide and the release of free 14C-Tyr and 14C-GlyNH2 was followed simultaneously with time by HPLC. Oxytocin was about four times more resistant to proteolysis than arginine-vasopressin as measured by slower disappearance of intact oxytocin, and reflected by the slower release of 14C-Tyr, but not of 14C-GlyNH2 from oxytocin. Comparison of degradation rates of structure analogues showed that peptides having Ile in position 3, as oxytocin, were more resistant than analogues having Phe in position 3, as arginine-vasopressin. The data demonstrate that arginine-vasopressin and oxytocin differ markedly in susceptibility to the aminopeptidase activity in brain synaptic membranes, and indicate that this difference resides primarily in the amino acid residue in position 3. It is suggested that the difference in susceptibility may affect the pattern of neurotrophic metabolites in brain. 相似文献
109.
Peter Miethe Ingeborg Jansen Uwe Niedermeyer Udo Kragl Regine Haftendorn Maria-Regina Kula Christian Wandrey Karl-Heinz Mohr Helmut-Walter Meyer 《Biocatalysis and Biotransformation》1992,7(1):61-73
The possibility of using the enzyme (R)-Oxynitrilase in a biphasic lyotropic liquid crystal/dibutylether system has been demonstrated. This reaction system is applicable for the continuous production of (R)-benzaldehydecyanohydrin in a fixed bed reactor. The optical purity was between 94 and 96% ee and independent of the flow rate. The space time yield was maximal (2650 g/(1*d)) at a flow rate of 1.6 ml/min. 相似文献
110.