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141.
Incorporation of photosynthetically fixed 14C was studied at different time intervals of 12, 24, and 36 h in various plant parts—leaf 1 to 4 from apex, roots, and rhizome—into primary metabolites—sugars, amino acids, and organic acids, and secondary metabolites—essential oil and curcumin—in turmeric. The youngest leaves were most active in fixing 14C at 24 h. Fixation capacity into primary metabolites decreased with leaf position and time. The primary metabolite levels in leaves were maximal in sugars and organic acids and lowest in amino acids. Roots as well as rhizome received maximum photoassimilate from leaves at 24 h; this declined with time. The maximum metabolite concentrations in the roots and rhizome were high in sugars and organic acids and least in amino acids. 14C incorporation into oil in leaf and into curcumin in rhizome was maximal at 24 h and declined with time. These studies highlight importance of time-dependent translocation of 14C-primary metabolites from leaves to roots and rhizome and their subsequent biosynthesis into secondary metabolite, curcumin, in rhizome. This might be one of factors regulating the secondary metabolite accumulation and rhizome development. 相似文献
142.
植被综合生态质量时空变化动态监测评价模型 总被引:3,自引:3,他引:3
为了能掌握全国植被综合生态质量的高低及其时空变化,构建了既能反映植被生产力又能反映植被覆盖度的植被综合生态质量指数,建立了植被综合生态质量指数年际对比和多年变化趋势评价模型。利用构建的指数和评价模型,以2017年作为监测评价的当年,以2000—2017年作为评价的多年时段,对全国植被综合生态质量时空变化进行了监测评价。结果表明:(1)2017年全国大部地区植被综合生态质量指数高于2000—2016年多年平均值,生态质量偏好;2017年福建、广西、海南、广东、云南植被综合生态质量位居全国前五位,构建的植被综合生态质量指数及其年际对比模型可以定量反映全国植被综合生态质量的空间差异和年际差异。(2)全国有90.7%的区域2000—2017年植被综合生态质量指数呈提高趋势,东北地区西部、内蒙古东部、华北大部、西北地区东部、西南地区东部、华南西部等地生态质量指数提升明显,构建的植被综合生态质量指数多年变化趋势评价模型可以定量反映植被生态质量的多年变化趋势和幅度。(3)南方大部地区2000—2017年平均年植被综合生态质量指数在50.0以上,北方大部地区在50.0以下;我国中东部大部地区在20.0以上,西部大部地区在20.0以下,表明南方大部地区年植被生态质量好于北方、中东大部好于西部。可见,构建的植被综合生态质量指数及其年际对比和多年变化趋势评价模型,能够监测评价当年和多年全国植被综合生态质量的时空变化,可为掌握全国植被生态质量动态提供模型和方法。 相似文献
143.
《Current biology : CB》2022,32(10):2272-2280.e6
144.
ESR spin trapping technique was used to detect and analyze free radical formation. When 6-hydroxydomine (6-OHDA) was incubated alone or in the presence of a free radical generating system (H2O2 and FeSO4), hydroxyl free radicals were observed in a concentration-dependent manner. Glutathione was found to be the most effective scavenger of the ESR signal when compared with vitamin E or Mannitol. The addition of ethanol resulted in the formation of the pure hydroxyethyl free radicals. The amount of hydroxyethyl free radicals in the system was dependent upon the concentration of ethanol and the formation of hydroxyethyl free radicals correlated well with the extent of lipid peroxidation and the loss of enzymic activity of the membrane-bound (Na+, K+)-ATPase. We suggest that in the biological system ethanol may potentiate the neurotoxicity of 6-OHDA with the formation of hydroxyethyl free radicals, which are longer-lived and far more damaging to membranes that the hydroxyl radicals. These data lead us to further hypothesize that the neuronal degeneration caused by 6-OHDA and other compounds that generate free radicals could be potentiated in the presence of ethanol. 相似文献
145.
Productivity is strongly associated with terrestrial species richness patterns, although the mechanisms underpinning such patterns have long been debated. Despite considerable consumption of primary productivity by fire, its influence on global diversity has received relatively little study. Here we examine the sensitivity of terrestrial vertebrate biodiversity (amphibians, birds and mammals) to fire, while accounting for other drivers. We analyse global data on terrestrial vertebrate richness, net primary productivity, fire occurrence (fraction of productivity consumed) and additional influences unrelated to productivity (i.e., historical phylogenetic and area effects) on species richness. For birds, fire is associated with higher diversity, rivalling the effects of productivity on richness, and for mammals, fire's positive association with diversity is even stronger than productivity; for amphibians, in contrast, there are few clear associations. Our findings suggest an underappreciated role for fire in the generation of animal species richness and the conservation of global biodiversity. 相似文献
146.
Wu‐Xia Qiu Xiao‐Li Ma Xiao Lin Fan Zhao Di‐Jie Li Zhi‐Hao Chen Ke‐Wen Zhang Ru Zhang Pai Wang Yun‐Yun Xiao Zhi‐Ping Miao Kai Dang Xiao‐Yang Wu Ai‐Rong Qian 《Journal of cellular and molecular medicine》2020,24(1):317-327
Microtubule actin cross‐linking factor 1 (Macf1) is a spectraplakin family member known to regulate cytoskeletal dynamics, cell migration, neuronal growth and cell signal transduction. We previously demonstrated that knockdown of Macf1 inhibited the differentiation of MC3T3‐E1 cell line. However, whether Macf1 could regulate bone formation in vivo is unclear. To study the function and mechanism of Macf1 in bone formation and osteogenic differentiation, we established osteoblast‐specific Osterix (Osx) promoter‐driven Macf1 conditional knockout mice (Macf1f/fOsx‐Cre). The Macf1f/fOsx‐Cre mice displayed delayed ossification and decreased bone mass. Morphological and mechanical studies showed deteriorated trabecular microarchitecture and impaired biomechanical strength of femur in Macf1f/fOsx‐Cre mice. In addition, the differentiation of primary osteoblasts isolated from calvaria was inhibited in Macf1f/fOsx‐Cre mice. Deficiency of Macf1 in primary osteoblasts inhibited the expression of osteogenic marker genes (Col1, Runx2 and Alp) and the number of mineralized nodules. Furthermore, deficiency of Macf1 attenuated Bmp2/Smad/Runx2 signalling in primary osteoblasts of Macf1f/fOsx‐Cre mice. Together, these results indicated that Macf1 plays a significant role in bone formation and osteoblast differentiation by regulating Bmp2/Smad/Runx2 pathway, suggesting that Macf1 might be a therapeutic target for bone disease. 相似文献
147.
利用2001-2010年EOS/MODIS17A3卫星遥感资料,对广西植被净初级生产力(NPP)时空特征及其影响因素进行分析.结果表明:(1)NPP 表现出明显的年际变化,2005年植被年均 NPP 最小为625 gC??m-2??a-1,2003年最大,为714 gC??m-2??a-1,十年间广西植被年NPP平均值为662 gC??m-2??a-1;(2)不同植被类型NPP有较大差异,森林、灌木、农作物的NPP 平均值分别为834、614、517 gC??m-2??a-1;(3)十年间广西区年均NPP为显著下降趋势,且年均气温和降水对NPP时间变化作用显著,而日照时数对 NPP 时间变化的作用不显著;(4)广西区NPP空间格局形成主要影响因素为坡度,其次为经度,再次为地貌特征、纬度和降水;(5)非喀斯特区域北热带季雨林、南亚热带季雨林化/季雨化常绿阔叶林年均 NPP 大于喀斯特地区,相反,喀斯特地区中亚热带常绿阔叶林,农作物年均NPP大于非喀斯特地区.整体而言,广西非喀斯特地区植被NPP为683 gC??m-2??a-1,喀斯特地区植被NPP为620 gC??m-2??a-1. 相似文献
148.
Hiroshi Fukamachi Shinko Kato Makoto Asashima Masao Ichinose Yasuhito Yuasa 《Development, growth & differentiation》2013,55(9):786-791
The importance of epithelial–mesenchymal interaction on the development of gastro‐intestinal (GI) organs has been repeatedly reported, but its molecular mechanism has not been fully understood though several factors including hepatocyte growth factor and endothelin‐3 have been shown to mediate it. Activins have been demonstrated to play important roles in the regulation of organogenesis in vertebrates, but their roles in the regulation of growth and differentiation of GI organs remain to be solved. In the present study, we examined expression of activins in developing rat GI tract, and found that inhibin bA encoding activin A was specifically expressed by GI mesenchymes, while inhibin bB encoding activin B was expressed by both epithelial and mesenchymal components. We then examined the effect of activin A on the growth of fetal rat GI epithelial cells in primary culture. We found that activin A inhibited the growth of forestomach and glandular stomach epithelial cells while it stimulated the growth of colonic epithelial cells. These results suggest that activin A secreted from GI mesenchymes region‐specifically regulates the growth of attaching epithelial cells. We thus conclude that activin A mediates epithelial‐mesenchymal interaction in the developing GI tract. 相似文献
149.
Jaekwang Jeong Ivonne Lisinski Anil K. G. Kadegowda Hyunsu Shin F. B. Peter Wooding Brian R. Daniels Jerome Schaack Ian H. Mather 《Traffic (Copenhagen, Denmark)》2013,14(9):974-986
Milk lipid is secreted by a unique process, during which triacylglycerol droplets bud from mammary cells coated with an outer bilayer of apical membrane. In all current schemes, the integral protein butyrophilin 1A1 (BTN) is postulated to serve as a transmembrane scaffold, which interacts either with itself or with the peripheral proteins, xanthine oxidoreductase (XOR) and possibly perilipin‐2 (PLIN2), to form an immobile bridging complex between the droplet and apical surface. In one such scheme, BTN on the surface of cytoplasmic lipid droplets interacts directly with BTN in the apical membrane without binding to either XOR or PLIN2. We tested these models using both biochemical and morphological approaches. BTN was concentrated in the apical membrane in all species examined and contained mature N‐linked glycans. We found no evidence for the association of unprocessed BTN with intracellular lipid droplets. BTN‐enhanced green fluorescent protein was highly mobile in areas of mouse milk‐lipid droplets that had not undergone post‐secretion changes, and endogenous mouse BTN comprised only 0.5–0.7% (w/w) of the total protein, i.e. over 50‐fold less than in the milk‐lipid droplets of cow and other species. These data are incompatible with models of milk‐lipid secretion in which BTN is the major component of an immobile global adhesive complex and suggest that interactions between BTN and other proteins at the time of secretion are more transient than previously predicted. The high mobility of BTN in lipid droplets marks it as a potential mobile signaling molecule in milk . 相似文献
150.
In the developing peanut (Arachis hypogaea L.) kernels, the period between 15 and 35 days after podding (DAP) was identified as the active period of oil-filling. The
period of active oil-filling was associated with a decrease in the starch, soluble sugars and proteins so as to make available
the energy and carbon skeleton for the synthesis of oil. The oil content in the mature kernels decreased by 11, 12 and 25
per cent with Zn, S and Zn+S deficiency, respectively. In addition, proteins and starch content decreased significantly while
that of soluble sugars increased slightly. The activity of malate dehydrogenase and glucose-6-phosphate dehydrogenase also
decreased due to Zn as well as S deficiency. The deficiency treatments resulted in a decrease in phospholipids, free fatty
acids and triacylglycerols in mature kernels. Further the proportion of 16∶0 and 18∶2 decreased while that of 18∶1 increased
in developing kernels. 相似文献