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121.
The antitumor effect of the combined administration with recombinant human interleukin-2 (rIL-2) and sizofiran (SPG), a single glucan of Shizophyllum commune Fries, was studied in vivo in C57BL/6 mice intraperitoneally inoculated with EL-4 lymphoma. The effect was evaluated by a) comparing the survival time of the mice, b) analysis of the intraperitoneal cell population in Giemsa-stained specimens, c) surface marker analysis of peritoneal exudative cells with flow cytometry, d) cytotoxic assay of cells against EL-4 and Yac-1 lymphoma, and e) elimination of some cell populations by monoclonal antibodies, to identify the antitumor-effector cells showing cytotoxic activity. The survival of mice given both rIL-2 and SPG was significantly longer than the control mice or those given SPG alone or rIL-2 alone. It was demonstrated that the administration of SPG and/or rIL-2 to the EL-4 lymphoma-bearing mice activated immune-response cells in the peritoneal cavity such as T lymphocytes, NK cells, or macrophages, which might be effective in reducing lymphoma cells. The combination of rIL-2 and SPG administration appears to activate the antitumor- immune response at the tumor site more effectively than when either agent was administered alone.  相似文献   
122.
猪精子凝集素的纯化,性质及其作用   总被引:3,自引:1,他引:2  
用胎球蛋白-Sepharose亲和层析和凝胶过滤层析从精子和精浆中分离纯化了猪精子凝集素(简称BSL)。BSL的血凝活性只被若干糖蛋白和聚糖所抑制。BSL的分子量为56kd,由分子量分别为13.6kd(β)和16.0kd(α)的两个不同的亚基以α1β3所组成。BSL为糖蛋白,含中性糖3.2%,不含唾液酸。用ELISA法测定猪精子中BSL的含量及分布,表明70%嵌入在精子膜中,25%结合在精子表面,  相似文献   
123.
以IL-8免疫的BALB/C小鼠脾细胞与Sp2/0或653小鼠骨髓瘤细胞融合构建了淋巴细胞杂交瘤克隆I8-S2和I8-63。ELISA叠加试验(ELISA Additivity Test)表明这两杂交瘤克隆分泌的单抗分别识别IL-8分子的不同表位。IL-8能激活人颗粒细胞,引起细胞内Ca~(2 )浓度([Ca~(2 )]_i)上升。通过流式细胞仪分析[Ca~(2 )]_i的变化,发现两个克隆单抗对IL8激活细胞的活力具有不同的中和作用。克隆I8-S2具有很强的中和作用,而克隆18-63则不然。上述结果提示IL-8的激活细胞活力局限于该分子的某表位。  相似文献   
124.
纯系间数量性状主基因差异的遗传分析   总被引:5,自引:0,他引:5  
将Elston模型应用于存在主基因差异的系间杂交的分离世代分析,提出利用似然函数分析数量性状的尺度效应、主基因分离比例以及主基因效应和微基因效应的方法,并应用于水稻遗传实验,结果表明,半矮秆籼稻品种南京11号带有的隐性矮秆主基因,效应大约为40-56cm,微基因效应以加性为主,且主、微型基因存在显著互作,但互作和微基因效应均远小于基因效应。  相似文献   
125.
Population-based data have not been readily available on relatively short-term changes in weight. Therefore, we sought to determine the nature of self-reported substantial (> 10%) weight change over one year in a representative sample of the US population which participated in the 1989 National Health Interview Survey (NHIS). Across all ages, a larger proportion of women than men reported both weight loss as well as weight gain of any amount (18.9% vs. 16.1% for weight loss and 20.0% vs. 16.1% for weight gain). In sex-specific logistic regression analyses, significant risk factors common to both sexes for substantial weight loss included divorced/separated marital status, smoking, increased number of blood pressure checks, increased BMI (body mass index) and increased number of bed days. Black race reduced the risk of weight loss for both men and women. Sex-specific risk factors for weight loss in men only were widowhood or never married marital status, while increasing age was a protective factor in women only. Concerning weight gain > 10% over the past year, increased number of blood pressure checks and having one or more diabetic parents were significant risk factors among both men and women; while never being married, increased age, BMI, and education exerted a protective effect in both sexes. For women only, risk factors for weight gain included black race, increased number of contacts with a health professional, and being unemployed. Intention to lose weight was associated with both weight gain and weight loss in both sexes, although it did not serve as a confounder in any of these relationships. A greater likelihood of substantial weight loss among women relative to men was diminished for persons with higher BMI, higher number of blood pressure checks, being widowed, divorced or separated, and intention to lose weight. A greater likelihood of substantial weight gain among women relative to men was diminished for persons with low BMI. The results of this cross-sectional study of weight change, involving a one-year follow-up period, generally correspond with the results obtained by longitudinal studies involving a longer follow-up.  相似文献   
126.
东北地区生态环境中的Se及其生态效应   总被引:3,自引:3,他引:0  
对东北地区不同环境要素Se的分布与转化规律的研究表明,基岩、土壤、粮食和动物毛Se含量分别为0.12、0.150-0.540、0.0096-0.0765和0.0405-0.1414μg.g^-1;城市和农村儿童发Se含量为0.460和0.182μg.g^-1。根据不同要素Se含量和生态效应,可将该区分为Se适宜区、缺乏区和过渡区3个1级区,每个1级区又分为高Se源区的低Se源区。  相似文献   
127.
腐殖酸对小麦抗旱性的生理效应   总被引:14,自引:2,他引:12  
在干旱气候条件下,喷施腐殖酸钠(HA)、黄腐酸钠(FA)可降低土壤水分损耗,提高小麦叶片持水能力,叶片细胞超氧物歧化酶(SOD)、过氧化氢酶(CAT)活性明显提高,丙二醛(MDA)含量和电解质渗出率明显降低,可减缓叶绿素降解,增强光合速率和光合产物积累,延缓植株衰老,小麦抗旱性增强,千粒重增加.  相似文献   
128.
苗雪梅  冯周琴 《蛇志》1995,7(3):4-6
采用青龙胶丸治疗脑梗塞后慢性脑功能不全50例。结果表明,在连续治疗3个月后,慢性脑功能不全所致的头痛、头晕、失眠、生活主动性减退、对周围事物反应能力减退等症状的有效率均达80%以上。神经心理学检查证实其对指向记忆、联想学习、人像特点联想回忆、认识能力等均有较好的改善作用。治疗前后血液流变学指标的改善,提示青龙胶丸降低纤维蛋白原、降低血液粘度,具有抗凝、解聚、溶栓、扩张血管、改善微循环的作用。认为是其对慢性脑功能不全发挥较好治疗作用的药理基础之一。  相似文献   
129.
中国茶对变形链球菌的抑菌作用   总被引:2,自引:0,他引:2  
对36只spraque-Dawley鼠分4组给予诱龋饲料(Diet2000,keys),并连续5天在口腔内接种变链菌6715菌株,分别给予我国主要产茶区的三种茶浸液:龙井绿茶(B组),四川涪陵红茶(C组),乌龙茶(D组),先用0.5g茶和50ml双蒸馏水加热煮沸过滤后浸泡10分钟以及对照组(A组)用双蒸馏水为饮料喂养实验鼠。实验60天后,杀鼠观察计算鼠磨牙面的菌落形成单位(CFU)和菌斑指数(plaquescore)均显著下降。实验结果,实验组和对照组菌斑指数下降值顺序如下:C组>D组>B组>A组,并与实验组含氟量有相关性。很多研究表明变链菌是较为肯定的致龋菌、茶中氟化物对变链菌生长、粘附都有抑制作用。本研究选用茶来源广泛、价廉、无毒安全等优点,为开拓新的防龋方法进行探索。  相似文献   
130.
Abstract. In order to explore whether seed size affects plant response to elevated CO2, plants grown from red oak (Quercus rubra L.) acorns were studied for differences in their first year response to CO2 concentrations of 350 and 700 μl/l. Overall, at final harvest, total biomass of plants grown in elevated CO2 were 47 % larger than that of plants grown in ambient CO2. There were significant interactions between CO2 treatments and initial acorn mass for total biomass, as well as for root, leaf, and stem biomass. Although total biomass increased with increasing initial acorn mass for both high and ambient CO2 plants, high CO2 plants exhibited a greater increase than ambient CO2 plants, as indicated by a steeper slope in high CO2 plants. However, CO2 levels did not affect biomass partitioning traits, such as root/shoot ratio, leaf, stem, and root weight ratios, and leaf area ratio. These results suggest that variation in seed size or initial plant size can cause intraspecific variation in response to elevated CO2.  相似文献   
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