全文获取类型
收费全文 | 9191篇 |
免费 | 648篇 |
国内免费 | 554篇 |
专业分类
10393篇 |
出版年
2023年 | 103篇 |
2022年 | 112篇 |
2021年 | 152篇 |
2020年 | 199篇 |
2019年 | 608篇 |
2018年 | 423篇 |
2017年 | 253篇 |
2016年 | 214篇 |
2015年 | 270篇 |
2014年 | 386篇 |
2013年 | 564篇 |
2012年 | 286篇 |
2011年 | 377篇 |
2010年 | 300篇 |
2009年 | 355篇 |
2008年 | 342篇 |
2007年 | 358篇 |
2006年 | 332篇 |
2005年 | 300篇 |
2004年 | 247篇 |
2003年 | 219篇 |
2002年 | 207篇 |
2001年 | 187篇 |
2000年 | 195篇 |
1999年 | 168篇 |
1998年 | 179篇 |
1997年 | 148篇 |
1996年 | 138篇 |
1995年 | 135篇 |
1994年 | 125篇 |
1993年 | 129篇 |
1992年 | 113篇 |
1991年 | 111篇 |
1990年 | 107篇 |
1989年 | 86篇 |
1988年 | 106篇 |
1987年 | 83篇 |
1986年 | 73篇 |
1985年 | 157篇 |
1984年 | 259篇 |
1983年 | 141篇 |
1982年 | 184篇 |
1981年 | 173篇 |
1980年 | 132篇 |
1979年 | 121篇 |
1978年 | 121篇 |
1977年 | 122篇 |
1976年 | 69篇 |
1975年 | 72篇 |
1974年 | 60篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
21.
22.
M. Masuyama 《Biometrical journal. Biometrische Zeitschrift》1991,33(6):725-733
A mathematical model of the linearized growth curve and its physiological interpretation by the introduction of proper age, which is proportional to the chronological age, are presented here. In the second phase, but not in the first phase, this constant of proportionality is highly correlated with the age at menarche. 相似文献
23.
ABSTRACT With countless “natural” experiments triggered by the COVID-19-associated physical distancing, one key question comes from chronobiology: “When confined to homes, how does the reduced exposure to natural daylight arising from the interruption of usual outdoor activities plus lost temporal organization ordinarily provided from workplaces and schools affect the circadian timing system (the internal 24 h clock) and, consequently, health of children and adults of all ages?” Herein, we discuss some ethical and scientific facets of exploring such natural experiments by offering a hypothetical case study of circadian biology. 相似文献
24.
Aorta smooth myosin contains two types of light chain, LC20 and LC17, which fold together with the N-terminal region of each heavy chain to form the globular head region of myosin. We demonstrate an altered conformation of LC20 after its separation from heavy chain by high concentrations of urea, on the basis of the following evidende: 1) A polyclonal antibody against LC20 was not able to recognize this conformationally altered form; 2) Myosin reconstituted from heavy chains and urea-dissociated light chains exhibited extremely low ATPase activity. Circular dichroism unfolding profiles showed that light chains dissociated from heavy chains by SDS appeared to be more stable than those generated by urea dissociation. 相似文献
25.
26.
Caroline S. Page Franz M. Matschinsky Paule E. Lacy Susan Conant 《Biochimica et Biophysica Acta (BBA)/General Subjects》1977,497(2):408-414
Electrophysiological studies of cultured rat pancreatic β-cells using intracellular microelectrodes show that exogenous insulin over the range of 0.1–10.0 μg/ml inhibits the electrical activity due to 27.8 mM glucose in a dose-related manner. This inhibitory effect is manifested by a mean increase of the membrane potential from about ?20 to ?30 mV and inhibition of the manner of cells impaled showing spike activity from 60 to less than 10%. The inhibitory influence of insulin is rapid occuring within 5 min for the highest level used. The results provide evidence for a negative feedback role of insulin in regulating its own release. 相似文献
27.
SYNOPSIS. A new species of Microsporida, Burenella dimorpha sp. n., representing a new family, Burenellidae fam. n. and genus, is described on the basis of light- and electron-microscope observations. The family is characterized by 2 sequences of sporogony, each sequence having morphologically different sporonts and spores. The parasite infects the tropical fire ant, Solenopsis geminata (Fabricius), producing distinct pathologic manifestations (clearing of the cuticle and eye malformation) and death in the pupal stage of development. Transmission of the infection per os to healthy S. geminata, to the Southern fire ant, Solenopsis xyloni McCook, and to the red and black imported fire ants, Solenopsis invicta Buren and Solenopsis richteri Forel, is reported. 相似文献
28.
The semidian rhythm in flowering response of Pharbitis nil in relation to dark period time measurement and to a circadian rhythm 总被引:1,自引:0,他引:1
When seedlings of Pharbitis nil Choisy, cv. Violet, are exposed to a single inductive dark period at 27°C, brief interruptions with red light (R) can be promotive after 2–3 h of darkness but increasingly inhibitory to flowering up to the 8–9th h of darkness. This rhythmic response to R interruptions can be advanced in phase by > 1 h when the preceding light period is interrupted with far-red (FR) 2 h before darkness (FR -2 h) or with FR – 15 h, whereas FR –8 h or FR–22 h retard the rhythm. These shifts in the R interruption rhythm are paralleled by equal shifts in the length of the dark period required for flowering. Brief FR interruptions of darkness displayed a similar rhythm which was also advanced by FR –2 h and retarded by FR –8 h. We conclude therefore that the semidian rhythm in the light, which we have previously described, continues through at least the first 12 h of darkness, is manifested in the R interruption rhythm, and determines the critical night length. A circadian rhythm with a marked effect on flowering was also identified, but several lines of evidence suggest that the circadian and semidian rhythms have independent additive effects on flowering and do not appear to show phase interaction. 相似文献
29.
Murine histocompatibility antigens were solubilized from the spleens and lungs of C57BL/6 (H-2b) animals with hypertonic salt (3 M KC1). Aggregate-free soluble antigens were incubated with nonadherent lymph node cells from BALB/c (H-2d) mice for 18 hr prior to their use as responder cells in the mixed-lymphocyte reaction (MLR). It was found that the generation of cytotoxic cells was suppressed while the proliferative response was not affected. The observed suppression was not due to a shift in the kinetics of the generation of cytotoxicity as determined throughout a 10-day culture period. The suppression was specific in that the response in MLR to unrelated H-2f stimulator cells and the subsequent generation of cytotoxic cells were unchanged. Using various H-2 recombinant strains as target cells in the assay of cell-mediated lympholysis, suppression of cytotoxicity was observed when the D end, but not the K end, was shared with the C57BL/6 strain from which the antigens were derived. 相似文献
30.
Many biomedical experiments require the qualitative and quantitative localization of trace elements with high sensitivity
and good spatial resolution. The feasibility of measuring the chemical form of the elements, the time course of trace element
metabolism, and conducting experiments in living biological systems are also important requirements for biological trace element
research. Nuclear analytical techniques that employ ion or photon beams have grown in importance in the past decade and have
led to several new experimental approaches. Some of the important features of these methods are reviewed here along with their
role in trace element research. Examples of their use are given to illustrate potential for new research directions. It is
emphasized that the effective application of these methods necessitates a closely integrated multidisciplinary scientific
team. 相似文献