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111.
Massimo Di Giulio 《Journal of molecular evolution》1997,45(6):571-578
A highly complex RNA world, as is sometimes presented in view of the widespread and diversified use of RNA enzymes, would
have encountered many difficulties in passing to a world with catalysis mediated by proteins. These difficulties can be overcome
by postulating a very early relationship between the nucleotide and the amino acid components. In particular, after asserting
that some characteristics expressed by (nucleotide) coenzymes in catalysis are easier to understand if a close and early relationship
between these coenzymes and amino acids is hypothesized, a model is presented for the origin of the enzyme–coenzyme complex.
This model is essentially based on an intermediate formed by a tRNA-like molecule covalently linked to a polypeptide. The
model attributes the majority of the catalytic role in the ribonucleoprotein world to the latter complex and thus it takes
into account the birth of the key intermediate in the origin of protein synthesis—namely, peptidyl-tRNA, which would have
otherwise been extremely difficult to select. The predictions of the model are discussed along with its robustness, using
the data derived from the study of intermediary metabolism and those from molecular biology. Finally, the appearance of the
genetic code in the late phase of the ribonucleopeptide world is discussed.
Received: 13 January 1997 / Accepted: 25 July 1997 相似文献
112.
Rates of development of stem nematode (Ditylenchus dipsaci) in white clover (Trifolium repens) seedlings were found to be linearly related to temperature. Basal developmental temperature (Tb) was 3 °C, and the thermal constant (S) for development of gravid adult females from freshly laid eggs was 270 accumulated day-degrees above the Tb. Only 12% at 20 °C and 4% at 4 °C of the gravid female nematodes inoculated into seedling axils successfully penetrated seedling epidermis. These nematodes slowly migrated within the seedling and after a lag of 5 days at 20 °C started to lay eggs. The maximal rate of egg production was temperature-dependent, being 0.8 and 3.1 eggs female⁻¹ day⁻¹ at 10 and 20 °C, respectively. Nematodes emigrated rapidly from infested stolons when they were immersed in water, with rates being highest at 25 °C and lowest at 4 °C. The sensitivity to temperature of many of the parameters that govern nematode population dynamics indicates that climatic changes will have a marked effect upon this host-parasite system. A study of infested stolons from the field indicated that nematode numbers increased up to 3,000 or more before tissue senesence, triggered by nematode damage, caused a mass emigration of nematodes from the stolon. 相似文献
113.
Reproduction and development of Pratylenchus penetrans were studied on genetically transformed ladino clover roots. Solitary females developing on transformed roots in nutrient gellan gum medium (pH 5.5) deposited 1.2, 1.5, 1.6, 1.8, and 2.0 eggs per day at the respective temperatures of 17, 20, 25, 27, and 30 °C. The number of eggs deposited was highly correlated with temperature. A reduction in egg-laying rates at the start of hatching was observed at all temperatures. Juvenile mortality was higher at 17 °C (50.4%), 20 °C (50.3%), and 30 °C (58.4%) than at 25 °C (34.6%) and 27 °C (37.6%). Life-cycle (egg deposition to egg deposition) duration was 46, 38, 28, 26, and 22 days at the respective temperatures. The developmental zero degrees (°C) and the effective accumulative temperatures (degree-days) required for hatching, female emergence, and onset of oviposition (completion of one generation) of P. penetrans were estimated to be 2.7 and 200, 4.2 and 548, and 5.1 and 564, respectively. Pratylenchus penetrans reproduces over a wide range of temperatures. 相似文献
114.
KOLOTKIN, RONETTE L, SUSAN HEAD, ALAN BROOKHART. Construct validity of the Impact of Weight on Quality of Life questionnaire. The Impact of Weight on Quality of Life questionnaire (IWQOL) is a 74-item self-report, condition-specific instrument that (1) assesses the effect of weight on quality of life in eight key areas, and (2) may be used as a treatment outcome measure and/or an evaluation tool for healthcare policy makers and third-party payers. This study explores IWQOL construct validity and provides new information on internal consistency, treatment effects, and differences between men and women. IWQOL total scores correlated highly with other measures of overall quality of life, and subscale scores correlated well with counterparts in the assessment battery. Internal consistency estimates for the IWQOL scales generally were high. For the women, 4-week participants, and the total sample, pretreatment-posttreatment differences were significant for all IWQOL scales and total score. For men, treatment differences were significant for the total score and all subscales except for Work and Mobility. Treatment differences for 2-week participants were significant for all scales except for Work. Consistent with previous IWQOL study results, the Comfort With Food scale scores reflected more discomfort at posttreatment as compared with pretreatment. The IWQOL, already translated into French and Italian, currently is demonstrating clinical and research utility as a quality-of-life outcome measure for clinical trials of antiobesity drugs and surgical treatments for patients with obesity. 相似文献
115.
三化螟种群系统的最优管理决策 总被引:1,自引:0,他引:1
以三化螟Tryporyza invertulas(Walker)种群动态模型和水稻产量损失预测模型为基础,根据水稻插植期、品种抗性,保护利用自然天敌和杀虫剂多次使用等控制措施以及它们的各种不同组合对该虫种群动态、水稻产量损失串和净收益的影响,以净收益最大为目标函数,研究三化螟种群的最优管理决策。其中,对昆虫种群动态模拟方法作了一点改进,它综合了前人所提出的种群动态模型的优点。建立的系统模型能够提供包括农业防治、生物防治和化学防治措施在内的、对三化螟种群实施有效管理的最优决策方案。 相似文献
116.
117.
Regio- and stereo-specific nitrile hydrolysis by the nitrile hydratase from Rhodococcus AJ270 总被引:1,自引:0,他引:1
Alan J.Blakey JohnColby EdwinWilliams CatherineO'Reilly 《FEMS microbiology letters》1995,129(1):57-61
Abstract Acid phosphatase activity was measured in individual cells by determining their optical densities through a scanning confocal laser microscope. The naphthol AS-TR (3-hydroxy-2-naphtoic acid 4'-chloro-2'-methylanilide) phosphate-hexazotized para-rosanilin method was used to visualise the acid phosphatase content in the light microscope. Evidence was obtained that the amount of enzyme varied in exponential growth phase cells as the fission age increased. By comparing the acid phosphatase activity with the rate of food vacuole formation, it appeared that the amount of enzyme inside the cells decreased in early clonal life, whereas the rate of food uptake increased. It was assumed that the reduction of acid phosphatase content could lead to a more extended life of vacuoles and to a decreased membrane recycling rate. In turn, the reduced supply of membrane available for food vacuole formation could partly be responsible for the decrease of the food uptake rate observed after the initial increase. 相似文献
118.
短尾猴种群生态学研究:Ⅲ.年龄结构和生命表 总被引:4,自引:4,他引:0
本文研究了黄山短猴(Macaca thibetana)种群年龄结构和雌性生命表。根据毛色、个体大小和行为等指标,可将其种群划分为6个年龄组,即婴猴组、少年组、青年组、年轻成年组、中年组和老年组。雌性短尾猴的静态生命表可较真实地反映了它在自然保护下的自下而上状况,其瞬时增长率(γ)为正值,表明只要加强对短尾猴现有栖息环境的保护,种群才会得到发展。 相似文献
119.
植物生活史对策的进化 总被引:19,自引:0,他引:19
植物生活史对策的进化班勇(中国林业科学院林业研究所,北京100091)EvolutionofLifeHistoryStrategyinPlants¥BanYong(ResearchInstituteofForestry,ChineseAcademyo... 相似文献
120.
四川55种鱼生活史型的研究 总被引:1,自引:0,他引:1
四川55种鱼生活史型的研究刁晓明,罗一兵李波(西南农业大学水产系,重庆630716)(重庆大学计算机系,630000)LifeHistoryPatternsof55FishSpeciesinSichuan¥DiaoXiaoming;LuoYibing... 相似文献