全文获取类型
收费全文 | 9634篇 |
免费 | 1489篇 |
国内免费 | 804篇 |
出版年
2024年 | 51篇 |
2023年 | 268篇 |
2022年 | 284篇 |
2021年 | 384篇 |
2020年 | 470篇 |
2019年 | 508篇 |
2018年 | 441篇 |
2017年 | 438篇 |
2016年 | 490篇 |
2015年 | 486篇 |
2014年 | 540篇 |
2013年 | 751篇 |
2012年 | 453篇 |
2011年 | 511篇 |
2010年 | 463篇 |
2009年 | 472篇 |
2008年 | 502篇 |
2007年 | 537篇 |
2006年 | 427篇 |
2005年 | 384篇 |
2004年 | 355篇 |
2003年 | 313篇 |
2002年 | 293篇 |
2001年 | 248篇 |
2000年 | 190篇 |
1999年 | 181篇 |
1998年 | 159篇 |
1997年 | 142篇 |
1996年 | 104篇 |
1995年 | 123篇 |
1994年 | 98篇 |
1993年 | 105篇 |
1992年 | 99篇 |
1991年 | 90篇 |
1990年 | 65篇 |
1989年 | 67篇 |
1988年 | 50篇 |
1987年 | 46篇 |
1986年 | 39篇 |
1985年 | 46篇 |
1984年 | 48篇 |
1983年 | 40篇 |
1982年 | 35篇 |
1981年 | 19篇 |
1980年 | 24篇 |
1979年 | 15篇 |
1978年 | 14篇 |
1977年 | 15篇 |
1976年 | 12篇 |
1975年 | 10篇 |
排序方式: 共有10000条查询结果,搜索用时 265 毫秒
71.
Clifford A. Siegfried 《Hydrobiologia》1985,122(2):175-180
The life history characteristics, population dynamics and production of Pontoporeia hoyi in Lake George, New York, were studied from May 1981 through October 1982. P. hoyi, in terms of both density and standing crop, is the most prevalent member of the deep water macrobenthos of Lake George. It reproduces in the winter, with young being released in the late winter-early spring. At the southernmost study site, young released in the spring grew to 6–7 mm in length and bred during their first winter. At the remaining sites, P. hoyi required two years to complete its life cycle. This difference in life history characteristics can be related to food availability and temperature differences. The open waters of the south end of Lake George are not only more productive but are also more closely associated with the littoral zone, providing a wealth of bacteria-rich detritus for benthic deposit feeders. The greater food availability in the south basin of Lake George is reflected in significantly larger brood sizes and smaller size at maturity for P. hoyi populations from the south end of the lake.The southernmost study site has significantly greater P. hoyi density and standing crop than all other sites. The cohort of the year dominated density and standing crop at the southern site while the cohort of the previous year dominated standing crop at the other sites. Peak abundance ranged from 600 · m–2 at the north site to 2 900 · m–2 at the south site. Cohort production ranged from 2g · m–2 at the north site to 15g · m–2 at the south site. 相似文献
72.
Five analogs of leucine enkephalin containing the CH2S group as an amide bond replacement were evaluated with respect to resistance toward degradation by human serum in an HPLC-based assay using both ultraviolet and electrochemical detection. Analogs with the modification at the 1-2, 2-3, 3-4, or 4-5 peptide linkages demonstrated half-lives of 118, 85, 134, and 318 min vs. 12 min for the parent peptide. A pseudopeptide analog with additional D-Ala2 protection had a half-life of greater than 1000 min, while the potent [D-Ala2]-leucine enkephalin analog showed approximately a 10-fold increase in stability. The significant increase in stability for a compound with protection only at the C-terminus suggests that serum enzymes may have greater specificity toward backbone changes than previously realized. 相似文献
73.
60-Hz electric fields: detection by female rats 总被引:1,自引:0,他引:1
Female rats were trained to detect a vertical, 60-Hz electric field using the same apparatus and procedure we used previously to study behavioral detection of the field by male rats. Each rat was trained individually to press a lever in the presence of the field and not to press in its absence. Correct detections occasionally produced a food pellet. The probability of detecting the field increased as field strength increased. The threshold of detection--ie, the field strength required for detections at a probability of 0.5 after correction for errors--varied among rats between 3 and 10 kV/m. Behavioral detection by female rats was indistinguishable from that by male rats. 相似文献
74.
The performance of The Subhuman Primate Pregnancy Test Kit was evaluated for routine detection of early (days 19-21) pregnancy in the rhesus monkey. Out of 123 confirmed matings, 19 resulted in pregnancy. In the pregnant animals the kit had an accuracy of 73.7%. In the nonpregnant females the accuracy was higher, 88.5%. False positives were encountered in ovariectomized females as well as adult intact males. 相似文献
75.
Synopsis Laboratory studies were conducted on 15 schools of blackchin shiners, Notropis heterodon, to determine if they altered their structure in response to changing environmental demands. The hypothesis tested was that fish schools should sacrifice a flat, hydrodynamically efficient structure in favour of an unobstructed visual field in the presence of a predator by staggering in the vertical plane. Ten schools were exposed for two weeks to a simple environment with only a current. For the next two-week period a predator was added. Five control schools were exposed to the simple environment for both two-week periods. Six of the ten treated schools increased their staggering in the vertical dimension as predicted while none of the control schools changed. This result was suggestive that hydrodynamic advantages were sacrificed. Respirometer experiments indicated these fish were capable of achieving some hydrodynamic benefits from schooling but these benefits may be a function of fish size. 相似文献
76.
An electrophoretic procedure for separating the molecular forms of catechol-O-methyltransferase in cellulose acetate gel is described; the zones of enzyme activity were revealed by autoradiography. The electrophoretic patterns of the enzyme in several tissues and cell lines derived from four different species are presented.This investigation was supported by Grant 500.6/Ric. 70/1981 from the Italian Ministry of Health and by the Italian Ministry of Education. We are grateful to Dr. M. Castagnola for useful advice and help with thin-layer chromatography. 相似文献
77.
Merritt Robert B. Kroon Wendy Hoffman Wienski Deborah A. Vincent Karen A. 《Biochemical genetics》1984,22(7-8):669-686
Genetic variation is described at 15 loci in 2 neotenic and 12 nonneotenic populations of red-spotted newts. Though high levels of genetic similarity (I=0.990) were found among all populations, allele frequencies at six of the eight most polymorphic loci show significant heterogeneity across populations. Change in allele frequencies at two of these loci (Pep-2 and Ldh-1) is significantly correlated with latitude. Interspecific homologies are established for newt peptidases based on substrate specificities and lactate dehydrogenases based on tissue distribution, thermal stability, and kinetic properties. Nonneotenic populations are highly variable (H=0.157) and neotenic populations are only slightly, but significantly, less variable (H=0.120). The high levels of heterozygosity detected in nonneotenic populations may result from large effective population size and/or environmental heterogeneity. The unexpectedly high heterozygosity values obtained for the neotenic populations may indicate adult dispersal or the presence of some previously undetected red efts at these localities. In any case, a major change in life history has apparently had little effect on the genetic structure of these populations.This research was supported by grants from the Blakeslee Fund of Smith College. 相似文献
78.
Summary Striated Ciliary Roots (SCRs), about 3 m long, are attached to the basal bodies of branchial crown ciliated epithelial cells ofOwenia. These SCRs appear to consist of 5–7-nm diameter filaments organized in a cross-striation pattern with an apparent variable periodicity of 50 to 80 nm. The most exciting observation emerging from this study is the constant and conspicuous close spatial relationship between SCRs and fairly well developed Golgi apparatus. By enhancing contrast and preservation of cell components, the OsFeCN postfixation-staining of material prefixed in glutaraldehyde in the presence of calcium has revealed some fine-structural details within the SCR-Golgi Association. By means of the calcium precipitation method, with antimonate or oxalate in conjunction with X-ray microanalysis, we have identified calcium within SCR dark bands and SCR-associated Golgi bodies. The ability to bind calcium makes the Golgi apparatus a likely candidate for Ca2+ regulation of putative contraction of the SCRs and/or ciliary motility. The slight period variability measured in the SCRs and cytochemical localization of Mg2+, Ca2+-dependent ATPase activities associated with cross striations support the view that theOwenia SCRs may be contractile organelles.The striking and specific close structural association between the Golgi apparatus and the SCR showing Ca2+-binding capabilities suggests that some sort of Ca2+-mediated functional relationship between these organelles may exist.Abbreviations SCR
striated ciliary root
- OsFeCN method
osmium tetroxide-ferricyanide method
- EDTA
ethylenediamine tetraacetic acid
- EGTA
ethyleneglycol-bis-(-aminoethyl ether) N,N-tetraacetic acid
- ATP
adenosine 5-triphosphoric acid
- ATPase
adenosine triphosphatase
- ASW
artificial sea-water 相似文献
79.
Breeding vervet monkeys in a closed environment 总被引:1,自引:0,他引:1
J V Seier 《Journal of medical primatology》1986,15(5):339-349
A breeding group of vervet monkeys produced 95 offspring over five years. Fetal wastage for 114 pregnancies was 16.7%. Uterine sizes were recorded for 103 pregnancies in various stages of gestation. This enabled diagnosis of pregnancy, estimation of stage of gestation, and prediction of parturition to within two weeks. Detailed observations were made on dental eruption, pelage development, and growth of 57 infants. 相似文献
80.
J. A. Horrocks 《International journal of primatology》1986,7(1):31-47
Life history data are presented for a population of vervets, Cercopithecusaethiops sabaeus, in Barbados, West Indies. The data were obtained from two habituated troops and from vervets captured during
a large-scale trapping program. Individuals of known age from one troop were weighed periodically, and separate growth curves
generated for males and females. The mean weight of captured adult females was 3.3 kg; that of adult males, 5.3 kg. The average
age at sexual maturity is estimated at 34 months for females and 60 months for males. Vervets give birth throughout the year,
but most infants are born between April and July. The average interbirth interval following a surviving infant is 11.8 months.
The mortality of juveniles is heaviest between birth and 2 years of age and decreases thereafter. Males emigrate from their
natal troops at sexual maturity and one incident of a juvenile female emigrating is reported. 相似文献