Quirks of dye nomenclature. 5. Rhodamines

Rhodamines were first produced in the late 19^th century, when they constituted a new class of synthetic dyes. These compounds since have been used to color many things including cosmetics, inks, textiles, and in some countries, food products. Certain rhodamine dyes also have been used to stain biological specimens and currently are widely used as fluorescent probes for mitochondria in living cells. The early history and current biological applications are sketched briefly and an account of the ambiguities, complications and confusions concerning dye identification and nomenclature are discussed.     

长脉冲1064nm激光联合酮康唑软膏封包病甲治疗甲真菌病的临床疗效观察

目的观察长脉冲1064 nm激光联合酮康唑软膏封包病甲治疗甲真菌病的临床疗效。方法 将73例甲真菌病门诊患者随机分为两组:联合组(37例,病甲75个)采用长脉冲1064 nm激光联合酮康唑软膏封包病甲治疗;对照组(36例,病甲87个)单纯使用激光治疗。每周1次,连续治疗8周,治疗6个月后随访评估。比较两种方法治疗甲真菌病的临床有效率,观察1064 nm激光联合酮康唑软膏封包病甲治疗甲真菌病患者的临床疗效。结果 联合组治疗甲真菌病的总的临床有效率(74.67%)高于对照组(63.22%),3种临床类型在联合组的临床有效率均高于对照组,尤其近端甲下型(PSO)的临床有效率明显提高,但差异无统计学意义;当9≤SCIO≤15时,联合组治疗甲真菌病的临床有效率高于对照组,差异有统计学意义。结论 对于9≤SCIO≤15的甲真菌病患者,长脉冲1064 nm激光联合酮康唑软膏封包病甲治疗甲真菌病的临床疗效显著高于单纯激光治疗。     

角膜屈光手术后屈光回退的手术治疗进展*

屈光回退是角膜屈光手术后的并发症之一,其治疗方法主要为药物治疗和手术治疗。对于需要再次手术的患者,应根据初次手术方式、距离初次手术时间、回退度数,在充分评价角膜情况后合理选择增效术,确保手术的安全性和有效性。目前,准分子激光原位角膜磨镶术和飞秒激光小切口角膜基质透镜取出术是治疗近视和近视散光的主要手术方式。本文就两者术后屈光回退手术治疗的适应症、不同增效术的优缺点及注意事项作一综述。     

脾脏保留手术对外伤性脾破裂患者免疫功能的影响

目的:探究脾脏保留手术对外伤性脾破裂患者免疫功能的影响。方法:选取2015年8月~2018年9月我院收治的外伤性脾破裂患者83例进行回顾性分析,根据手术方式不同分为两组,对照组(41例)患者给予脾脏切除术,观察组(42例)患者给予脾脏保留手术。比较两组患者的手术时间、术中出血量、下床活动时间、术后1d引流量、抢救成功率及治疗前后CD3~+、CD4~+、CD8~+和Tuftsin因子水平和并发症的发生情况。结果:治疗后,观察组患者的手术时间、术中出血量、术后下床时间和术后1d引流量均显著短于或低于对照组,而救治成功率显著高于对照组(P0.05)。两组患者治疗后的CD3~+、CD4~+和CD4~+/CD8~+水平均较治疗前显著下降,且观察组以上指标均显著高于对照组(P0.05)。对照组治疗后血清Tuftsin因子水平较治疗前显著下降,而观察组血清Tuftsin因子水平较治疗前显著升高,并显著高于对照组(P0.05)。观察组患者的总并发症发生率为7.14%,较对照组(24.39%)显著降低(P0.05)。结论:与脾脏切除术相比,脾脏保留手术可显著改善外伤性脾破裂患者的免疫功能,且手术效果更好,安全性更高。     

经皮激光汽化术联合杜仲腰痛丸治疗腰椎间盘突出症的效果观察

目的:研究经皮激光汽化术联合杜仲腰痛丸治疗腰椎间盘突出症的临床效果。方法:选择2016年4月~2018年4月我院脊柱骨科收治的106例腰椎间盘突出症患者,随机分为两组。对照组单独采用经皮激光汽化术治疗,观察组联合口服杜仲腰痛丸治疗,每次8粒,每天3次。比较两组的治疗有效率,治疗前后的VAS评分、JOA评分,血清白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平的改变情况。结果:治疗后,观察组的治疗有效率为88.68%(47/53),明显高于对照组[73.58%(39/53)](P0.05)。两组治疗后的VAS评分值均较治疗前明显降低(P0.05),JOA评分值均较对照组明显升高(P0.05),且观察组VAS评分值明显低于对照组,JOA评分值显著高于对照组(P0.05)。两组治疗后的血清IL-1β、TNF-α水平均较治疗前明显降低(P0.05),且观察组血清IL-1β、TNF-α水平明显低于对照组(P0.05)。两组均未发生神经损伤和无椎间盘炎等并发症。结论:经皮激光汽化术联合杜仲腰痛丸治疗腰椎间盘突出症的临床效果明显优于单独采用经皮激光汽化术治疗,其可以显著改善患者的生活质量,降低疼痛程度,其作用机制可能与有降低患者血清炎症介质IL-1β、TNF-α的表达有关。     

Novel dye for detection of callus embryo by confocal laser scanning fluorescence microscopy

In the present study a new luminescent dye 3‐N‐(2‐pyrrolidinylacetamido)benzanthrone (AZR) was synthesized. Spectroscopic measurements of the novel benzanthrone 3‐aminoderivative were performed in seven organic solvents showing strong fluorescence. The capability of the prepared dye for visualization has been tested on flax, red clover and alfalfa to determinate the embryo in plant callus tissue cultures. Callus cells were stained with AZR and further analysed utilizing confocal laser scanning fluorescence microscopy. Performed experiments show high visualization effectiveness of newly synthesized fluorescent dye AZR that is efficient in fast and relatively inexpensive diagnostics of callus embryos that are problematic due to in vitro culture specificity.     

Quantitative Microproteomics Based Characterization of the Central and Peripheral Nervous System of a Mouse Model of Krabbe Disease

1. Download : Download high-res image (139KB)
2. Download : Download full-size image

Highlights

• •Quantitative microproteomics to study the CNS and PNS of the Twitcher mouse.
• •10plex TMT experiments on corpus callosum, motor cortex and sciatic nerves extracts.
• •More than 400 proteins groups deregulated between Twitcher and wildtype mice.
• •New insights into the molecular mechanisms of Krabbe disease.

     

Facile Patterning of Laser‐Induced Graphene with Tailored Li Nucleation Kinetics for Stable Lithium‐Metal Batteries

A facile and scalable approach is reported to stabilize the lithium‐metal anode by regulating the Li nucleation and deposition kinetics with laser‐induced graphene (LIG). By processing polyimide (PI) films on copper foils with a laser, a 3D‐hierarchical composite material is constructed, consisting of a highly conductive copper substrate, a pillared array of flexible PI, and most importantly, porous LIG on the walls of the PI pillars. The high number of defects and heteroatoms present in LIG significantly lowers the Li nucleation barrier compared to the copper foil. An overpotential‐free Li nucleation process is identified at current densities lower than 0.2 mA cm^?2. Theoretical computations reveal that the defects serve as nucleation centers during the heterogeneous nucleation of lithium. By adopting such composites, ultrastable lithium‐metal anodes are obtained with high Coulombic efficiencies of ≈99%. Full lithium‐metal cells based on LiFePO₄ cathodes with a material loading of ≈15 mg cm^?2 and a negative/positive ratio of 5/1 could be cycled over 250 times with a capacity loss of less than 10%. The current work highlights the importance of nucleation kinetics on the stability of metallic anodes and demonstrates a practical method toward long lasting Li‐metal batteries.     

Bioimaging of multiple elements by high‐resolution LA‐ICP‐MS reveals altered distribution of mineral elements in the nodes of rice mutants

Inter‐vascular transfer in rice (Oryza sativa) nodes is required for delivering mineral elements to developing tissues, which is mediated by various transporters in the nodes. However, the effect of these transporters on distribution of mineral elements in the nodes at a cellular level is still unknown. Here, we established a protocol for bioimaging of multiple elements at a cellular level in rice node by laser ablation‐inductively coupled plasma‐mass spectrometry (LA‐ICP‐MS), and compared the mineral distribution profile between wild‐type (WT) rice and mutants. Both relative comparison of mineral distribution normalized by endogenous ¹³C and quantitative analysis using spiked standards combined with soft ablation gave valid results. Overall, macro‐nutrients such as K and Mg were accumulated more in the phloem region, while micro‐nutrients such as Fe and Zn were highly accumulated at the inter‐vascular tissues of the node. In mutants of nodal Zn transporter OsHMA2, Zn localization pattern in the node tissues did not differ from that of WT; however, Zn accumulation in the inter‐vascular tissues was lower in uppermost node I but higher in the third upper node III compared with the WT. In contrast, Si deposition in the mutants of three nodal Si transporters Lsi2, Lsi3 and Lsi6 showed different patterns, which are consistent with the localization of these transporters. This improved LA‐ICP‐MS analysis combined with functional characterization of transporters will provide further insight into mineral element distribution mechanisms in rice and other plant species.     

An interactive surgical simulation tool to assess the consequences of a partial glossectomy on a biomechanical model of the tongue

Oral cancer surgery has a negative influence on the quality of life (QOL). As a result of the complex physiology involved in oral functions, estimation of surgical effects on functionality remains difficult. We present a user-friendly biomechanical simulation of tongue surgery, including closure with suturing and scar formation, followed by an automated adaptation of a finite element (FE) model to the shape of the tongue. Different configurations of our FE model were evaluated and compared to a well-established FE model. We showed that the post-operative impairment as predicted by our model was qualitatively comparable to a patient case for five different tongue maneuvers.