An Extended,Problem-Based Learning Laboratory Exercise on the Diagnosis of Infectious Diseases Suitable for Large Level 1 Undergraduate Biology Classes

The development and evaluation of a two-week laboratory class, based on the diagnosis of human infectious diseases, is described. It can be easily scaled up or down, to suit class sizes from 50 to 600 and completed in a shorter time scale, and to different audiences as desired. Students employ a range of techniques to solve a real-life and relevant problem, and are introduced to the range and type of infectious agents, their routes of transmission and risk factors, clinical symptoms and diagnoses, and their treatment and prevention. No infectious material is used, and the practical is very inexpensive and easy to prepare. Six ‘patients’ are diagnosed, using their symptoms, patient histories, temperature records, serology, blood and faecal slide examination, and bacteriological isolation from blood, faeces and cerebrospinal fluid.     

Cryo-EM Structure of the Full-length hnRNPA1 Amyloid Fibril

Heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) is a multifunctional RNA-binding protein that is associated with neurodegenerative diseases, such as amyotrophic lateral sclerosis and multisystem proteinopathy. In this study, we have used cryo-electron microscopy to investigate the three-dimensional structure of amyloid fibrils from full-length hnRNPA1 protein. We find that the fibril core is formed by a 45-residue segment of the prion-like low-complexity domain of the protein, whereas the remaining parts of the protein (275 residues) form a fuzzy coat around the fibril core. The fibril consists of two fibril protein stacks that are arranged into a pseudo-2₁ screw symmetry. The ordered core harbors several of the positions that are known to be affected by disease-associated mutations, but does not encompass the most aggregation-prone segments of the protein. These data indicate that the structures of amyloid fibrils from full-length proteins may be more complex than anticipated by current theories on protein misfolding.     

Integrating information literacy training into an inquiry-based introductory biology laboratory

ABSTRACT

Information literacy is an essential skill for biologists; however, most biology curricula do not intentionally integrate information literacy into classroom and laboratory exercises. There is evidence that developing information literacy skills in undergraduates improves their research skills, writing, and GPAs. Our objective was to integrate information literacy skills into a first semester introductory biology laboratory with a multi-week, inquiry-based module that leverages primary literature. Here we describe the module, which challenges students to develop and test a hypothesis related to parental care behaviour in birds. Students form hypotheses based on literature searching done during librarian-led information literacy sessions, produce an annotated bibliography, collect and analyse video data of barn swallows feeding their offspring, and present their findings. Analysis of students’ annotated bibliographies indicates that 83% of the referenced papers were appropriate for developing their specific hypotheses. The key elements ofa successful information literacy training plan include faculty-librarian collaboration, multiple classroom or laboratory sessions that introduce or utilize information literacy, and relevance ofthe information literacy training to an assignment. By introducing information literacy early inbiology curricula, departments can develop tiered information literacy plans that incorporate opportunities for students to use and refine these skills throughout their studies.     

Laboratory studies of the effects of short‐term isolation on aggressive behaviour in fish

A review of the literature on the effects of short‐term isolation on aggressive behaviour in a variety of fishes reveals different approaches and often conflicting results. This diversity is attributed to a number of factors, including different experimental conditions and specific differences in habitat, behavioural repertoire and social system. Some common trends are identified and the main hypotheses proposed to account for the different results are discussed. Some methodological issues as well as proximate and ultimate mechanisms are examined, and the importance of a standard experimental paradigm in comparative research emphasised.     

Laboratory models,causal explanation and group selection

We develop an account of laboratory models, which have been central to the group selection controversy. We compare arguments for group selection in nature with Darwin's arguments for natural selection to argue that laboratory models provide important grounds for causal claims about selection. Biologists get information about causes and cause-effect relationships in the laboratory because of the special role their own causal agency plays there. They can also get information about patterns of effects and antecedent conditions in nature. But to argue that some cause is actually responsible in nature, they require an inference from knowledge of causes in the laboratory context and of effects in the natural context. This process, cause detection, forms the core of an analogical argument for group selection. We discuss the differing roles of mathematical and laboratory models in constructing selective explanations at the group level and apply our discussion to the units of selection controversy to distinguish between the related problems of cause determination and evaluation of evidence. Because laboratory models are at the intersection of the two problems, their study is crucial for framing a coherent theory of explanation for evolutionary biology.     

Evaluation of Occupational Injuries among Young Workers in West Virginia

We compared workplace injuries between young (16 to 19 years of age) and adult workers using West Virginia Workers' Compensation database. All workers injured between January 1 and December 31, 1995 were included in the analysis. The industry-specific injury incidence rates between young and adults workers were significantly different with lower rates of injury in young workers in all sectors except service sector. In the service sector the young workers had significantly higher injury rates than adults (rate ratios for young workers were 2.28, 1.92, and 2.94 when compared with age groups 20-24, 25-34, and >34, respectively). Estimates of the proportional injury ratio (PIR) indicated significantly greater risk of finger (PIR 1.62) and hand (PIR 1.66) injuries and burns (PIR 3.27) and lacerations (PIR 1.69) in the young workers. The proportion of injuries occurring in the summer months was higher in the young than in the adults (35.2% vs. 27.0%), particularly in the service sector (79.6% vs. 25.9%). Higher injury rates in young workers compared to adults in the service sector may be explained by the seasonal employment of young workers in West Virginia.