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981.
Mary Ann Lila Smith L. Art Spomer 《In vitro cellular & developmental biology. Plant》1987,23(1):67-70
Summary Objective, accurate, non-intrusive measurement of in vitro cell growth was realized through microcomputerized video image
analysis. Recently-released video and digitizing hardware and software were incorporated into an analytical system which accurately
quantified visual differences between cultures on a cell number or fresh mass basis. Sequential measurements during culture
incubation further detected and quantified subtle changes in colony area and density resulting from growth. Each measurement
was acquired rapidly, without encroaching on the in vitro environment, so cell growth was undisturbed. Custom software routines
coordinated the quantification of this detailed record into precise cumulative growth curves. 相似文献
982.
《Expert review of proteomics》2013,10(4):321-323
Evaluation of: Ghosh D, Li Z, Tan XF, Lim TK, Mao Y, Lin Q. RAQ based quantitative proteomics approach validated the role of calcyclin binding protein (CacyBP) in promoting colorectal cancer metastasis. Mol. Cell Proteomics 12(7), 1865–1880 (2013).The use of iTRAQ-based relative quantification is demonstrated by Ghosh et al. to analyze the downstream effectors of a calcyclin-binding protein, which is proposed to promote colorectal cancer progression. These findings are reviewed and discussed in relation to the need to establish robust in vitro model cell lines for identification of cancer-specific pathways and to confirm the leads generated by proteomic analysis. 相似文献
983.
Christian Genty Sabine Palle Laurence Vanelle Sandrine Bourrin Christian Alexandre 《Biotechnic & histochemistry》1994,69(3):160-164
We have developed a colorimetric method for evaluating the number of osteoblastic cells in culture without destroying the cells. This assay is based on the staining of basophilic cellular compounds with methylene blue. The dye bound by the cells is released at low pH and measured in a spectrophotometer at 662 nm. Linear correlations exist between the absorbance measured by the methylene blue assay and the number of cells seeded, the total cellular protein content, and thymidine labeling. This colorimetric method has the advantage of preserving cell integrity. After destaining, scanning electron microscopy can be performed on well preserved cell morphology. 相似文献
984.
Subcellular Distribution of Tyrosine Hydroxylase in Some Catecholaminergic Rat Brain Areas Determined by a Quantitative Immunoblot Assay 总被引:2,自引:2,他引:0
The subcellular distribution of the protein tyrosine hydroxylase (TH) after fractionation of rat brain tissue was studied by a sensitive technique of immunoblot quantification in the dopaminergic nigrostriatal and the dorsal noradrenergic pathways and in the ventrolateral medulla. This repartition indicates that in all catecholaminergic regions of the cell bodies studied, the contribution of the nerve endings to the total TH amount is very low (less than 7%), in contrast to that observed in the terminal fields. The correlative subcellular determination of the TH amount and activity in the same tissue could be a useful approach for studying experimentally induced mechanisms of catecholamine synthesis modulation in different brain catecholaminergic pathways. 相似文献
985.
986.
Susanne H. Kirsch Jean-Pierre KnappWolfgang Herrmann Rima Obeid 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2010,878(1):68-75
Folates act as essential coenzymes in many biological pathways. Alteration in folate form distribution might have biological significance, especially in relation to certain genetic polymorphisms. We developed a stable-isotope dilution ultra performance liquid chromatography–mass spectrometry (UPLC–MS/MS) method for quantification of the folate forms 5-methyltetrahydrofolate (5-methylTHF), 5-formylTHF, 5,10-methenylTHF, THF, and folic acid in serum. After extraction using an ion exchange and mixed mode solid-phase, samples were separated and detected using an UPLC–MS/MS system. The quantification limits were between 0.17 nmol/L (5-formylTHF) and 1.79 nmol/L (THF), and the assay was linear up to 100 nmol/L (5-methylTHF) and 10 nmol/L (5-formylTHF, 5,10-methenylTHF, THF, and folic acid). The intraassay CVs for 5-methylTHF and 5-formylTHF were 2.0% and 7.2%, respectively. Mean recoveries were between 82.3% for THF and 110.8% for 5,10-methenylTHF. Concentrations of total folate measured by the new method showed a strong correlation with those measured by an immunologic assay (r = 0.939; p < 0.001). The mean total folate from 32 apparently healthy subjects was 18.09 nmol/L, of which 87.23% was 5-methylTHF. Concentrations of homocysteine showed a better correlation to the total folate measured by the new method compared to that obtained by an immunologic assay. We also confirmed that MTHFR polymorphism has a significant effect on folate distribution in this small population of non-supplemented subjects. 相似文献
987.
A non-destructive method for the relative growth quantification of algae or cyanobacteria in the culture has been developed.
It is based on image analysis and does not require any special equipment. Results provided by this method were compared with
those provided by a chlorophyll a assessment, with a correlation factor of over 88%. 相似文献
988.