全文获取类型
收费全文 | 62134篇 |
免费 | 18708篇 |
国内免费 | 2768篇 |
专业分类
83610篇 |
出版年
2024年 | 115篇 |
2023年 | 482篇 |
2022年 | 585篇 |
2021年 | 1176篇 |
2020年 | 3577篇 |
2019年 | 5133篇 |
2018年 | 5292篇 |
2017年 | 5344篇 |
2016年 | 5025篇 |
2015年 | 4869篇 |
2014年 | 5047篇 |
2013年 | 5910篇 |
2012年 | 4555篇 |
2011年 | 4970篇 |
2010年 | 4267篇 |
2009年 | 3390篇 |
2008年 | 3640篇 |
2007年 | 2989篇 |
2006年 | 2888篇 |
2005年 | 2367篇 |
2004年 | 1992篇 |
2003年 | 2002篇 |
2002年 | 1677篇 |
2001年 | 1338篇 |
2000年 | 910篇 |
1999年 | 696篇 |
1998年 | 371篇 |
1997年 | 319篇 |
1996年 | 270篇 |
1995年 | 265篇 |
1994年 | 254篇 |
1993年 | 224篇 |
1992年 | 218篇 |
1991年 | 188篇 |
1990年 | 148篇 |
1989年 | 125篇 |
1988年 | 108篇 |
1987年 | 103篇 |
1986年 | 116篇 |
1985年 | 79篇 |
1984年 | 96篇 |
1983年 | 78篇 |
1982年 | 109篇 |
1981年 | 60篇 |
1980年 | 76篇 |
1979年 | 46篇 |
1978年 | 39篇 |
1977年 | 16篇 |
1976年 | 18篇 |
1974年 | 12篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
51.
52.
Growth and death rates of aboveground plant parts were measured in a mature forest and four different-aged deciduous broadleaf forests regeneratede after clear-cutting, with special reference to rates for woody parts (stems and branches) of different diameters (ø) in rerms of the pipe model theory (Shinozaki et al., 1964). The total biomass increment of woody parts of trees higher than 1.3 m varied within a range of 2.1-4.6 ton ha?1 yr?1, the increase beingdue largely to the growth of canopy trees exposed to direct sunlight. Biomass increments of small (ø<1 cm) and medium (1≤ø<5 cm) woody parts were negligibly small except in the youngest forest, and changes in aboveground woody biomass with forest age after clear-cutting mainly resulted from accumulation of large (5 cm<ø) woody parts of canopy trees. Biomass loss of trees due to death and grazing increased with forest age from 4.0 to 8.3 ton ha?1 yr?1. Recovery of leaf and small wood falls was observed at the early stage of regeneration, while large wood falls increased during regeneration. Flower and fruit fall was markedly higher in the mature forest than in the other four forest types. Mortality of woody parts became higher with forest age and was 20, 5.0 and 0.46% yr?1 for small, medium and large parts, respectively, at the mature stage. Aboveground net production of the forest was in therange 7.6-13.3 ton ha?1 yr?1 with the undergrowth vegetation lower than 1.3 m being 0.4-1.4 ton ha?1 yr?1. Production recovered rapidly at an early stage of regeneration and was highest in mature forest. 相似文献
53.
James M. May 《The Journal of membrane biology》1989,108(3):227-233
Summary The sulfhydryl reagent 5, 5-dithiobis (2-nitrobenzoic acid) (DTNB) was used to study the functional role of an exofacial sulfhydryl group on the human erythrocyte hexose carrier. Above 1mm DTNB rapidly inhibited erythrocyte 3-O-methylglucose influx, but only to about half of control rates. Efflux was also inhibited, but to a lesser extent. Uptake inhibition was completely reversed by incubation and washing with 10mm cysteine, whereas it was only partially reduced by washing in buffer alone, suggesting both covalent and noncovalent interactions. The covalent thiol-reversible reaction of DTNB occurred on the exofacial carrier, since (i) penetration of DTNB into cells was minimal, (ii) blockade of potential uptake via the anion transporter did not affect DTNB-induced hexose transport inhibition, and (iii) DTNB protected from transport inhibition by the impermeant sulfhydryl reagent glutathione-maleimide-I. Maltose at 120mm accelerated the covalent transport inhibition induced by DTNB, whereas 6.5 m cytochalasin B had the opposite effect, indicating under the one-site carrier model that the reactive sulfhydryl is on the outward-facing carrier but not in the substrate-binding site. In contrast to glutathione-maleimide-I, however, DTNB did not restrict the ability of the carrier to reorient inwardly, since it did not affect equilibrium cytochalasin B binding. Thus, carrier conformation determines exposure of the exofacial carrier sulfydryl, but reaction of this group may not always lock the carrier in an outward-facing conformation. 相似文献
54.
Adam Bertl 《The Journal of membrane biology》1989,109(1):9-19
Summary The membrane of mechanically prepared vesicles ofChara corallina has been investigated by patch-clamp techniques. This membrane consists of tonoplast as demonstrated by the measurement of ATP-driven currents directed into the vesicles as well as by the ATP-dependent accumulation of neutral red. Addition of 1mm ATP to the bath medium induced a membrane current of about 3.2 mA·m–2 creating a voltage across the tonoplast of about –7 mV (cytoplasmic side negative). On excised tonoplast patches, currents through single K+-selective channels have been investigated under various ionic conditions. The open-channel currents saturate at large voltage displacements from the equilibrium voltage for K+ with limiting currents of about +15 and –30 pA, respectively, as measured in symmetric 250mm KCl solutions. The channel is virtually impermeable to Na+ and Cl–. However, addition of Na+ decreases the K+ currents. TheI–V relationships of the open channel as measured at various K+ concentrations with or without Na+ added are described by a 6-state model, the 12 parameters of which are determined to fit the experimental data. 相似文献
55.
This paper describes a set of mathematical models to calculate runoff, soil erosion and chemical substance yields for drainage areas. The models represent different degrees of complexity and details of describing hydrological and hydrochemical processes. The availability of initial information and the requirements of the task determine the choice of a model for each particular case. These models have been written as PC/AT programs, to enable their use by a wide range of specialists. The models have been tested in different physical and geographical conditions, e.g. in some small watersheds on the Karelian Isthmus, west of Lake Ladoga, and in experimental catchment areas located in the Valdai Hills area in the southern part of Lake Ladoga drainage area. In further applications the drainage area models have been combined with models of the recipient water body, to obtain a single model of a watershed-lake system. The results of tests have proved that the models can be used for evaluation, simulation and forecasting of the processes of rainfall and snowmelt runoff, infiltration, evaporation, water erosion and pollutant wash-off from drainage areas. 相似文献
56.
Synopsis One perspective emphasizing the importance of stochastic processes in determining coral reef fish assemblages implies that there is little organization in species richness, abundance structure, and spatial distribution. We examine the degree to which this perspective is correct by analyzing distribution of fishes on a collection of patch reefs (Discovery Bay, Jamaica). We ask the question whether these patches accumulate species and individuals in a manner consistent with stochastic expectations. To address this question we use two conceptual models, each permitting a different insight. One assumes that fish are distributed stochastically on patches while the other assumes presence of restrictions on fish distribution due to habitat structure. For each conceptual model we use two types of benchmark: we compare observed patterns to those predicted by theoretical models, and we also compare observed patterns to those obtained from a random reallocation of fish individuals to patches. We found that the conceptual model assuming stochastic processes appeared to provide weaker explanation of patterns than the conceptual model that includes restrictions due to habitat structure. Further, and more importantly, we found that (i) the community is shaped by a mixture of stochastic and non-stochastic mechanisms, and (ii) the stochastic assembly processes decrease in importance for species restricted to fewer microhabitat types and sites. Our study therefore indicates that patches do accumulate individuals and species in a manner consistent with stochastic expectations, however, this applies primarily to the habitat generalists (unrestricted species). By the same token, increased habitat specialization by some species imposes constraints on the stochastic model such that it eventually fails. 相似文献
57.
Summary We present a simple model of habitat selection in which individuals differ in their ability to discriminate between resource sites' profitabilities. The model investigates the effects of violating the ideal assumption of the well-known ideal free distribution (IFD). We show that (1) variability in perceptual limits within a population can significantly change the distribution of foraging animals even though the mean perceptual limit is the same, (2) the direction of this change depends on the proportion of the population that choose randomly between resource sites and (3) better perceivers are more likely to be found at individually more profitable sites, which, because of undermatching with respect to the IFD, are also the absolutely more profitable sites. We note that variability in perceptual limits almost always led to an undermatching of organisms to resources, thereby extending previous workers' results implying that the incorporation of any form of perceptual limits leads to undermatching with respect to the IFD. 相似文献
58.
59.
M. C. Léglise P. Darodes de Tailly J. L. Vignot M. A. Le Bot A.-M. Le Roux C. Riché 《Cell biology and toxicology》1996,12(1):39-53
A cellular model of hematopoiesis which would be more convenient than bone marrow (BM) progenitors and directly relevant to human pathology is needed in order to investigate xenobiotic toxicity. Human umbilical cord blood (HCB), previously shown to be able to repopulate BM, provides a powerful in vitro model of normal human hematopoiesis. In order to validate the use of normal HCB progenitors as targets for dose-related myelosuppression, we used clonogenic assays and expansion in a liquid culture of progenitor-enriched cell suspensions from HCB. A series of 8 reference molecules, doxorubicin, cytosine-arabinoside, 5-fluorouracil, 3-azido-3-deoxythymidine, acetylsalicyclic acid, sodium valproate and two cephalosporin antibiotics, were tested. In vitro 50% inhibition concentrations (IC50) were compared to those observed or reported with BM progenitors, and to the values of plasma concentrations from treated patients. HCB progenitors as in vitro targets for cytotoxic molecules were easy to access and handle, and their use was sensitive, specific and reproducible. They gave results similar to BM progenitors and allowed a qualitative approach to cellular metabolism and toxicity using morphological, flow cytometric and chromatographic methods.Abbreviations ARA-CC
cytosine arabinoside
- AS
acetylsalicylic acid
- AZTT
3-azido-3-deoxythymidine
- BFUU
burst-forming units
- BM
bone marrow
- CFU
colony-forming units
- DOXX
doxorubicin
- FU
5-fluorouracil
- glyAA
glyAcophorin A
- HCB
human umbilical cord blood
- IU
international units
- PCMEM
human placenta-conditioned medium
- VA
sodium valproate 相似文献
60.
Christoph Meier Walter Senn Rudolf Hauser Manfred Zimmermanne 《Journal of mathematical biology》1994,32(6):563-572
The classical Nicholson-Bailey model for a two species host-parasitoid system with discrete generations assumes random distributions of both hosts and parasitoids, randomly searching parasitoids, and random encounters between the individuals of the two species. Although unstable, this model induced many investigations into more complex host-parasitoid systems. Local linearized stability analysis shows that equilibria of host parasitoid systems within the framework of a generalized Nicholson-Bailey model are generally unstable. Stability is only possible if host fertility does not exceede
4=54.5982 and if superparasitism is unsuccessful. This special situation has already been discovered by Hassell et al. (1983) in their study of the effects of variable sex ratios on host parasitoid dynamics. We discuss global behaviour of the Hassell-Waage-May model using KAM-theory and illustrate its sensitivity to small perturbations, which can give rise to radically different patterns of the population dynamics of interacting hosts and parasitoids. 相似文献