全文获取类型
收费全文 | 5002篇 |
免费 | 460篇 |
国内免费 | 842篇 |
出版年
2024年 | 15篇 |
2023年 | 111篇 |
2022年 | 109篇 |
2021年 | 161篇 |
2020年 | 248篇 |
2019年 | 263篇 |
2018年 | 265篇 |
2017年 | 310篇 |
2016年 | 323篇 |
2015年 | 304篇 |
2014年 | 295篇 |
2013年 | 354篇 |
2012年 | 197篇 |
2011年 | 244篇 |
2010年 | 190篇 |
2009年 | 243篇 |
2008年 | 274篇 |
2007年 | 282篇 |
2006年 | 286篇 |
2005年 | 232篇 |
2004年 | 221篇 |
2003年 | 186篇 |
2002年 | 219篇 |
2001年 | 153篇 |
2000年 | 116篇 |
1999年 | 114篇 |
1998年 | 106篇 |
1997年 | 77篇 |
1996年 | 60篇 |
1995年 | 73篇 |
1994年 | 53篇 |
1993年 | 30篇 |
1992年 | 43篇 |
1991年 | 21篇 |
1990年 | 22篇 |
1989年 | 18篇 |
1988年 | 13篇 |
1987年 | 10篇 |
1986年 | 9篇 |
1985年 | 8篇 |
1984年 | 8篇 |
1983年 | 4篇 |
1982年 | 13篇 |
1981年 | 2篇 |
1980年 | 6篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1977年 | 2篇 |
1974年 | 2篇 |
1958年 | 2篇 |
排序方式: 共有6304条查询结果,搜索用时 15 毫秒
991.
Exotic species typically lose most of their associated parasites during long-distance spread. However, the few parasites that are co-introduced may have considerable adverse impacts on their novel hosts, including mass mortalities. We present a comprehensive inventory of parasites known to infect 38 species of exotic invertebrates established in the Great Lakes, as well as 16 invertebrate species predicted to arrive in the near future, all of them crustaceans. Based on a literature analysis, we identified a total of 277 parasite taxa associated with the examined invertebrates in their native ranges and/or invaded areas. Of these parasites, 56 species have been documented to cause various pathologies in their intermediate or final hosts, with humans and fishes being the most frequently affected host categories. Potentially harmful parasites were identified in 61% of the invaders for which published information was retrieved (in their ranges outside of the Great Lakes), with molluscs and crustaceans hosting the highest numbers of such parasites. The results of our study provide a baseline for further assessment and management of the parasitological risks posed by exotic species to the Great Lakes. 相似文献
992.
Joana Almeida Wouter M.J. Achten Bruno Verbist Reindert F. Heuts Eddie Schrevens Bart Muys 《Journal of Industrial Ecology》2014,18(6):898-908
This study reports on the carbon, water, and energy footprints of tomatoes grown in a greenhouse in Northern Italy and two possible future variations of heating and carbon dioxide (CO2) fertilization on the current setup. The heat supply in place, consisting of natural gas (NG) and canola oil combustion, is compared to cogeneration and incineration of municipal solid waste for heating and CO2 from industrial exhaust for fertilization. As a benchmark, the current system is also compared to a conventional system, in which heat is delivered solely based on NG. Each kilogram (kg) of fresh tomatoes (“Cuore di Bue” variety) produced in the current greenhouse emits 2.28 kg CO2 equivalents (eq) and uses 95.5 megajoules (MJ) eq energy and 122 liters (L) of water. Relative to the system in place, the carbon footprint (CF) is 57.5% and 18% higher with conventional NG heating and cogeneration and is 40% lower with waste valorization. Further, 33%, 55%, and 63% less energy and 9%, 96%, and 14% less water are used in the conventional, cogeneration, and waste valorization scenarios, respectively. This confirms that there are multiple strategies to reduce the impact of the tomato production under consideration. 相似文献
993.
Ecosystem models play a crucial role in understanding and evaluating the combined impacts of rising atmospheric CO2 concentration and changing climate on terrestrial ecosystems. However, we are not aware of any studies where the capacity of models to simulate intra‐ and inter‐annual variation in responses to elevated CO2 has been tested against long‐term experimental data. Here we tested how well the ecosystem model APSIM/AgPasture was able to simulate the results from a free air carbon dioxide enrichment (FACE) experiment on grazed pasture. At this FACE site, during 11 years of CO2 enrichment, a wide range in annual plant production response to CO2 (?6 to +28%) was observed. As well as running the full model, which includes three plant CO2 response functions (plant photosynthesis, nitrogen (N) demand and stomatal conductance), we also tested the influence of these three functions on model predictions. Model/data comparisons showed that: (i) overall the model over‐predicted the mean annual plant production response to CO2 (18.5% cf 13.1%) largely because years with small or negative responses to CO2 were not well simulated; (ii) in general seasonal and inter‐annual variation in plant production responses to elevated CO2 were well represented by the model; (iii) the observed CO2 enhancement in overall mean legume content was well simulated but year‐to‐year variation in legume content was poorly captured by the model; (iv) the best fit of the model to the data required all three CO2 response functions to be invoked; (v) using actual legume content and reduced N fixation rate under elevated CO2 in the model provided the best fit to the experimental data. We conclude that in temperate grasslands the N dynamics (particularly the legume content and N fixation activity) play a critical role in pasture production responses to elevated CO2, and are processes for model improvement. 相似文献
994.
Daniele Colombaroli Immaculate Ssemmanda Vanessa Gelorini Dirk Verschuren 《Global Change Biology》2014,20(9):2903-2914
Rainfall controls fire in tropical savanna ecosystems through impacting both the amount and flammability of plant biomass, and consequently, predicted changes in tropical precipitation over the next century are likely to have contrasting effects on the fire regimes of wet and dry savannas. We reconstructed the long‐term dynamics of biomass burning in equatorial East Africa, using fossil charcoal particles from two well‐dated lake‐sediment records in western Uganda and central Kenya. We compared these high‐resolution (5 years/sample) time series of biomass burning, spanning the last 3800 and 1200 years, with independent data on past hydroclimatic variability and vegetation dynamics. In western Uganda, a rapid (<100 years) and permanent increase in burning occurred around 2170 years ago, when climatic drying replaced semideciduous forest by wooded grassland. At the century time scale, biomass burning was inversely related to moisture balance for much of the next two millennia until ca. 1750 ad , when burning increased strongly despite regional climate becoming wetter. A sustained decrease in burning since the mid20th century reflects the intensified modern‐day landscape conversion into cropland and plantations. In contrast, in semiarid central Kenya, biomass burning peaked at intermediate moisture‐balance levels, whereas it was lower both during the wettest and driest multidecadal periods of the last 1200 years. Here, burning steadily increased since the mid20th century, presumably due to more frequent deliberate ignitions for bush clearing and cattle ranching. Both the observed historical trends and regional contrasts in biomass burning are consistent with spatial variability in fire regimes across the African savanna biome today. They demonstrate the strong dependence of East African fire regimes on both climatic moisture balance and vegetation, and the extent to which this dependence is now being overridden by anthropogenic activity. 相似文献
995.
996.
997.
Binnur Eroglu Donald E. Kimbler Junfeng Pang Justin Choi Demetrius Moskophidis Nathan Yanasak Krishnan M. Dhandapani Nahid F. Mivechi 《Journal of neurochemistry》2014,130(5):626-641
Traumatic brain injury (TBI) induces severe harm and disability in many accident victims and combat‐related activities. The heat‐shock proteins Hsp70/Hsp110 protect cells against death and ischemic damage. In this study, we used mice deficient in Hsp110 or Hsp70 to examine their potential requirement following TBI. Data indicate that loss of Hsp110 or Hsp70 increases brain injury and death of neurons. One of the mechanisms underlying the increased cell death observed in the absence of Hsp110 and Hsp70 following TBI is the increased expression of reactive oxygen species‐induced p53 target genes Pig1, Pig8, and Pig12. To examine whether drugs that increase the levels of Hsp70/Hsp110 can protect cells against TBI, we subjected mice to TBI and administered Celastrol or BGP‐15. In contrast to Hsp110‐ or Hsp70i‐deficient mice that were not protected following TBI and Celastrol treatment, there was a significant improvement of wild‐type mice following administration of these drugs during the first week following TBI. In addition, assessment of neurological injury shows significant improvement in contextual and cued fear conditioning tests and beam balance in wild‐type mice that were treated with Celastrol or BGP‐15 following TBI compared to TBI‐treated mice. These studies indicate a significant role of Hsp70/Hsp110 in neuronal survival following TBI and the beneficial effects of Hsp70/Hsp110 inducers toward reducing the pathological consequences of TBI.
998.
Meike Engelbrecht Esther Bochet Patricio García‐Fayos 《Biological journal of the Linnean Society. Linnean Society of London》2014,111(2):241-251
Diaspores of many plant species inhabiting open vegetation in semi‐arid environments secrete mucilage after wetting (myxospermy) that glues the diaspores to the ground and prevents movement when the mucilage dries. In the present study, we test whether mucilage secretion can be considered as a selective response to soil erosion in plant species inhabiting semi‐arid environments. We relate the amount and type of mucilage secretion by seeds of Helianthemum violaceum and Fumana ericifolia (Cistaceae) to the number of raindrop impacts needed to remove these seeds after gluing them with their own mucilage to the ground and also the time that these seeds resist water run‐off without detaching. We also compare the amount of seed mucilage production by plants growing in habitats without erosion and plants affected by severe erosion by fitting mixed effect models. Our results show an important phenotypic variation in the amount of mucilage secretion in both species, although it is suggested that the effect of mucilage secretion in the rate of seed removal by erosion is species‐ and mechanism‐dependent. For F. ericifolia, the amount of mucilage secreted by the seeds is directly proportional to their resistance to raindrop impacts and is positively related to the intensity of the erosive processes that the plants experience. Nevertheless, all the seeds resist the force of run‐off during 60 min, irrespective of the amount of mucilage they produce. In H. violaceum, mucilage secretion per se, and not the amount of mucilage produced by the seeds, has an effect on the rate of seed removal by erosive processes. Furthermore, cellulosic fibrils were found only in the mucilage of F. ericifolia but not in H. violaceum. Overall, our results only partially support the hypothesis that a selective response to soil erosion exists. © 2013 The Linnean Society of London, Biological Journal of the Linnean Society, 2014, 111 , 241–251. 相似文献
999.
Jay Rasmussen Brandon H Gilroyed Tim Reuter Sandor Dudas Norman F Neumann Aru Balachandran 《朊病毒》2014,8(1):136-142
Prions, the causative agent of chronic wasting disease (CWD) enter the environment through shedding of bodily fluids and carcass decay, posing a disease risk as a result of their environmental persistence. Plants have the ability to take up large organic particles, including whole proteins, and microbes. This study used wheat (Triticum aestivum L.) to investigate the uptake of infectious CWD prions into roots and their transport into aerial tissues. The roots of intact wheat plants were exposed to infectious prions (PrPTSE) for 24 h in three replicate studies with PrPTSE in protein extracts being detected by western blot, IDEXX and Bio-Rad diagnostic tests. Recombinant prion protein (PrPC) bound to roots, but was not detected in the stem or leaves. Protease-digested CWD prions (PrPTSE) in elk brain homogenate interacted with root tissue, but were not detected in the stem. This suggests wheat was unable to transport sufficient PrPTSE from the roots to the stem to be detectable by the methods employed. Undigested PrPTSE did not associate with roots. The present study suggests that if prions are transported from the roots to the stems it is at levels that are below those that are detectable by western blot, IDEXX or Bio-Rad diagnostic kits. 相似文献
1000.
A statistical simulation model for field testing of non‐target organisms in environmental risk assessment of genetically modified plants 下载免费PDF全文
Paul W. Goedhart Hilko van der Voet Ferdinando Baldacchino Salvatore Arpaia 《Ecology and evolution》2014,4(8):1267-1283
Genetic modification of plants may result in unintended effects causing potentially adverse effects on the environment. A comparative safety assessment is therefore required by authorities, such as the European Food Safety Authority, in which the genetically modified plant is compared with its conventional counterpart. Part of the environmental risk assessment is a comparative field experiment in which the effect on non‐target organisms is compared. Statistical analysis of such trials come in two flavors: difference testing and equivalence testing. It is important to know the statistical properties of these, for example, the power to detect environmental change of a given magnitude, before the start of an experiment. Such prospective power analysis can best be studied by means of a statistical simulation model. This paper describes a general framework for simulating data typically encountered in environmental risk assessment of genetically modified plants. The simulation model, available as Supplementary Material, can be used to generate count data having different statistical distributions possibly with excess‐zeros. In addition the model employs completely randomized or randomized block experiments, can be used to simulate single or multiple trials across environments, enables genotype by environment interaction by adding random variety effects, and finally includes repeated measures in time following a constant, linear or quadratic pattern in time possibly with some form of autocorrelation. The model also allows to add a set of reference varieties to the GM plants and its comparator to assess the natural variation which can then be used to set limits of concern for equivalence testing. The different count distributions are described in some detail and some examples of how to use the simulation model to study various aspects, including a prospective power analysis, are provided. 相似文献