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排序方式: 共有589条查询结果,搜索用时 15 毫秒
51.
Toumazou C Cass T 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2007,362(1484):1321-1328
The accurate monitoring of the physiological status of cells, tissues and whole organisms demands a new generation of devices capable of providing accurate data in real time with minimal perturbation of the system being measured. To deliver on the promise of cell-bionics advances over the past decade in miniaturization, analogue signal processing, low-power electronics, materials science and protein engineering need to be brought together. In this paper we summarize recent advances in our research that is moving us in this direction. Two areas in particular are highlighted: the exploitation of the physical properties inherent in semiconductor devices to perform very low power on chip signal processing and the use of gene technology to tailor proteins for sensor applications. In the context of engineered tissues, cell-bionics could offer the ability to monitor the precise physiological state of the construct, both during 'manufacture' and post-implantation. Monitoring during manufacture, particularly by embedded devices, would offer quality assurance of the materials components and the fabrication process. Post-implantation monitoring would reveal changes in the underlying physiology as a result of the tissue construct adapting to its new environment. 相似文献
52.
Hye-Min Kim You-Kyung Chang Soo-In Ryu Sung-Gweon Moon Soo-Bok Lee 《Journal of Molecular Catalysis .B, Enzymatic》2007,49(1-4):98-103
Pyrococcus horikoshii trehalose-synthesizing glycosyltransferase employed a galactose as an acceptor in the glucosyl transfer reaction with an NDP-Glc donor. The reaction produced a non-reducing transfer product in a yield of more than 30% based on the molar concentration of donor used. The transfer product was purified by paper chromatography and preparative HPLC, and its glycosidic structure was confirmed by 13C nuclear magnetic resonance to be -d-glucopyranosyl -d-galactopyranoside. Interestingly, this trehalose analogue disaccharide inhibited the action of several disaccharidases, including a trehalase. The analogue competitively inhibited porcine kidney and rat intestinal trehalases with Ki values of 0.68 and 3.7 mM, respectively. It also competitively inhibited other intestinal disaccharidases such as sucrase, maltase, and isomaltase with respective Ki values of approximately 0.66, 3.0, and 2.1 mM. Accordingly, this trehalose analogue would be a potentially indigestible disaccharide, effectively inhibiting intestinal brush border disaccharidases. 相似文献
53.
van der Schoot SC Nuijen B Flesch FM Gore A Mirejovsky D Lenaz L Beijnen JH 《AAPS PharmSciTech》2007,8(3):E78-E87
The purpose of this research was to develop a stable bladder instillation of EO-9 for the treatment of superficial bladder
cancer. First, stability and dissolution studies were performed. Subsequently, the freeze-drying process was optimized by
determination of the freeze-drying characteristics of the selected cosolvent/water system and differential scanning calorimetry
analysis of the formulation solution. Furthermore, the influence of the freeze-drying process on crystallinity and morphology
of the freeze-dried product was determined with x-ray diffraction analysis and scanning electron microscopy, respectively.
Subsequently, a reconstitution solution was developed. This study revealed that tert-butyl alcohol (TBA) can be used to both
dramatically improve the solubility and stability of EO-9 and to shorten the freeze-drying cycle by increasing the sublimation
rate. During freeze drying, 3 TBA crystals were found: TBA hydrate-ice crystals, crystals of TBA hydrate, and a third crystal,
probably composed of TBA hydrate crystals containing ≈90% to 95% TBA. Furthermore, it was shown that crystallization of TBA
hydrate was inhibited in the presence of both sodium bicarbonate (NaHCO3) and mannitol. Addition of an annealing step resulted in a minor increase in the crystallinity of the freeze-dried product
and formation of the δ-polymorph of mannitol. A stable bladder instillation was obtained after reconstitution of the freeze-dried
product (containing 8 mg of EO-9, 20 mg of NaHCO3, and 50 mg of mannitol per vial) to 20 mL with a reconstitution solution composed of propylene glycol/water for injection
(WfI)/NaHCO3/sodium edetate 60%/40%/2%/0.02% vol/vol/wt/wt, followed by dilution with Wfl to a final volume of 40 mL.
Published: August 3, 2007 相似文献
54.
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56.
Banchet-Cadeddu A Martinez A Guillarme S Parietti V Monneaux F Hénon E Renault JH Nuzillard JM Haudrechy A 《Bioorganic & medicinal chemistry letters》2011,21(8):2510-2514
Our goal in the search for potentially bioactive analogues of KRN 7000 was to design an easy synthetic approach to a library of analogues using a strategy recently developed in our laboratory based on a Nucleophilic addition followed by an Epoxide Opening (the NEO strategy). Through the use of a common pivotal structure, a new C-galactoside ester analogue (23) was synthesized which showed an encouraging TH2 biased response during preliminary biological tests. 相似文献
57.
Xiao Q Liu Y Qiu Y Yao Z Zhou G Yao ZJ Jiang S 《Bioorganic & medicinal chemistry letters》2011,21(12):3613-3615
A new series of linear dimeric compounds mimicking naturally occurring annonaceous acetogenins have been synthesized by bivalent analogue design, and their cytotoxicities have been evaluated against the growth of cancer cells by MTT method. Most of these compounds show selective action favored to human cancer cell lines over normal cell lines, and compound 9 with bis-terminal benzoquinone functionality exhibits an IC50 = 0.40 μM against MCF7 cell lines. This work mentions that appropriate conformational constraints might be a useful optimizing tool for this unique class of anticancer compounds. 相似文献
58.
A fluorescent fatty acid probe, DAUDA, selectively displaces two myristates bound in human serum albumin 总被引:1,自引:0,他引:1
Wang Y Luo Z Shi X Wang H Nie L Huang M 《Protein science : a publication of the Protein Society》2011,20(12):2095-2101
11-(Dansylamino) undecanoic acid (DAUDA) is a dansyl-type fluorophore and has widely used as a probe to determine the binding site for human serum albumin (HSA). Here, we reported that structure of HSA-Myristate-DAUDA ternary complex and identified clearly the presence of two DAUDA molecules at fatty acid (FA) binding site 6 and 7 of HSA, thus showing these two sites are weak FA binding sites. This result also show that DAUDA is an appropriate probe for FA site 6 and 7 on HSA as previous studied, but not a good probe of FA binding site 1 that is likely bilirubin binding site on HSA. 相似文献
59.
Here we present first dinucleotide affinity resins for purification of proteins that specifically recognize the 5' end of mRNA. Constructed resins possess either a naturally occurring mono- or trimethylated cap or their analogues resistant towards enzymatic degradation, bearing a CH(2) bridge between β and γ position of the 5',5'-triphosphate chain. All cap analogues were attached to a polymer support (EAH-Sepharose) through the carboxylic group that had been generated by derivatization of the 2',3'-cis diol of the second nucleotide in the cap structure with levulinic acid. 相似文献
60.
抗吡虫啉单克隆抗体的制备及鉴定 总被引:1,自引:0,他引:1
为制备灵敏度高,特异性强的抗吡虫啉单克隆抗体,建立经济、快速、准确的吡虫啉残留免疫学分析方法,采用分子模拟技术分析吡虫啉及其类似农药的电荷分布后,选择1-[6-(2-羧乙硫基-3-吡啶)甲基]-N-硝基-2-咪唑啉亚胺 (H1) 作为免疫半抗原,1-(6-氯-3-吡啶甲基)-3-羧丙基-N-硝基-2-咪唑啉亚胺 (H2) 作为包被半抗原,利用NHS酯法将H1和H2分别与牛血清蛋白 (BSA) 和卵清蛋白 (OVA) 偶联合成免疫原与包被原。免疫BALB/c小鼠后,采用常规杂交瘤技术共获得2株稳定分泌抗吡虫 相似文献