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Host‐plant associated genetic divergence of two Diatraea spp. (Lepidoptera: Crambidae) stemborers on novel crop plants 下载免费PDF全文
Andrea L. Joyce Miguel Sermeno Chicas Leopoldo Serrano Cervantes Miguel Paniagua Sonja J. Scheffer M. Alma Solis 《Ecology and evolution》2016,6(23):8632-8644
Diatraea lineolata and Diatraea saccharalis (Lepidoptera: Crambidae) are moths with stemboring larvae that feed and develop on economically important grasses. This study investigated whether these moths have diverged from a native host plant, corn, onto introduced crop plants including sorghum, sugarcane, and rice. Diatraea larvae were collected from these four host plants throughout the year in El Salvador and were reared on artificial diet until moths or parasitoids emerged. Adult moths were subsequently identified to species. Amplified fragment length polymorphisms (AFLPs) and mitochondrial DNA cytochrome oxidase I (COI) were used to examine whether or not there was genetic divergence of D. lineolata or D. saccharalis populations on the four host plants. Percent parasitism was also determined for each moth on its host plants. D. lineolata was collected from corn in the rainy season and sorghum in the dry season. D. saccharalis was most abundant on sugarcane in the rainy season and sorghum in the dry season. The AFLP analysis found two genetically divergent populations of both D. lineolata and D. saccharalis. Both moths had high levels of parasitism on their dominant host plant in the rainy season, yet had low levels of parasitism on sorghum in the dry season. The presence of two genotypes of both Diatraea spp. on sorghum suggest that host‐associated differentiation is occurring on this novel introduced crop plant. 相似文献
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Effects of heat and drought stress on post‐illumination bursts of volatile organic compounds in isoprene‐emitting and non‐emitting poplar 下载免费PDF全文
Werner Jud Elisa Vanzo Ziru Li Andrea Ghirardo Ina Zimmer Thomas D. Sharkey Armin Hansel Jörg‐Peter Schnitzler 《Plant, cell & environment》2016,39(6):1204-1215
Over the last decades, post‐illumination bursts (PIBs) of isoprene, acetaldehyde and green leaf volatiles (GLVs) following rapid light‐to‐dark transitions have been reported for a variety of different plant species. However, the mechanisms triggering their release still remain unclear. Here we measured PIBs of isoprene‐emitting (IE) and isoprene non‐emitting (NE) grey poplar plants grown under different climate scenarios (ambient control and three scenarios with elevated CO2 concentrations: elevated control, periodic heat and temperature stress, chronic heat and temperature stress, followed by recovery periods). PIBs of isoprene were unaffected by elevated CO2 and heat and drought stress in IE, while they were absent in NE plants. On the other hand, PIBs of acetaldehyde and also GLVs were strongly reduced in stress‐affected plants of all genotypes. After recovery from stress, distinct differences in PIB emissions in both genotypes confirmed different precursor pools for acetaldehyde and GLV emissions. Changes in PIBs of GLVs, almost absent in stressed plants and enhanced after recovery, could be mainly attributed to changes in lipoxygenase activity. Our results indicate that acetaldehyde PIBs, which recovered only partly, derive from a new mechanism in which acetaldehyde is produced from methylerythritol phosphate pathway intermediates, driven by deoxyxylulose phosphate synthase activity. 相似文献
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为了研究红缘天牛成虫对寄主植物杏树衰弱树及木段挥发物的生理及行为反应,本文用6种挥发物,分别以不同浓度的单一组份对红缘天牛进行了电生理和行为测试;又在这6种单组份化合物对天牛行为选择表现为引诱活性的浓度范围内选择EAG值最高的浓度,以4-6种化合物等体积制成7种组合配方进行EAG和行为测试。结果表明:单一组份R-柠檬烯、反-2-己烯醛、丁酸丁酯、S-柠檬烯、异戊醇和3-蒈烯等6种单一化合物在预设的8个浓度梯度范围内,都能引起红缘天牛产生一定的生理反应,但行为选择实验的结果没有统计学意义。7种组合配方中,R4对红缘天牛成虫引诱活性最强(P0.01),引诱率达到76.67%,R1次之(P0.05),R4与R1的区别在于增加了丁酸丁酯;即丁酸丁酯在R4配方中具有明显的增效作用。 相似文献
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梨小食心虫Grapholita molesta(Busck),是一种世界性的蛀果害虫。利用产卵选择试验研究了15种寄主植物挥发物对梨小食心虫受孕雌虫的产卵引诱活性。室内试验表明:受孕雌虫对不同挥发物的产卵选择趋性不同,其中对叶醇和己酸丁酯表现出明显的产卵选择趋性。为验证寄主挥发物对受孕雌虫的实际诱捕效果,进行了田间诱捕试验。结果发现,挥发物中己酸丁酯、苯甲腈、叶醇、E-2-己烯醛以及乙酸己酯诱捕到的雌虫均为受孕雌虫,显著高于空白对照及其他物质,其中己酸丁酯的平均诱捕量最多,苯甲腈次之,E-2-己烯醛、乙酸己酯这两种物质的平均诱捕量分别为第三、第四,叶醇的诱捕量低于乙酸己酯,其余物质的诱捕量均较低。综合室内及田间试验结果发现,寄主植物挥发物对受孕雌虫的引诱作用也随它们之间的作用距离表现出一定的差异性。 相似文献
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Olfactory cues explain differential attraction of the striped rice stem borer to different varieties of rice plant 下载免费PDF全文
In Iranian rice fields, different varieties of rice are cultivated which are differentially impacted by females of the rice stem borer, Chilo suppressalis. To elucidate the role odours may play in their host plant finding behaviour, female Ch. suppressalis were exposed to four varieties of rice plants and their volatiles in a four‐arm olfactometer. In whole plant tests, Ch. suppressalis were significantly attracted to the variety previously characterized as most susceptible, least attracted to one characterized as semisusceptible, and showed no attraction to those varieties characterized as semi‐ and highly resistant. Tests using headspace volatile extracts yielded similar results in the case of the most susceptible variety, but showed no attraction to the semisusceptible and highly resistant varieties, and low attraction to the semiresistant variety. Subsequent analysis of the volatile composition identified a panel of 27 components, some of which were either unique to, or abundantly present in, particular varieties, and may explain the observed variation in their attractiveness. Our findings show that rice plant volatiles can play a role in the host selection behaviour of this pest species, and we suggest compounds which may be important to this process and the future application of volatiles in rice pest management programs. 相似文献