Comparison of surface topography of three species of Diphyllobothrium (Cestoda,pseudophyllidea) by scanning electron microscopy

Plerocercoids of different sizes as well as adult worms of D. dendriticum, D. latum and D. ditremum were studied using scanning electron microscopy (S.E.M.). In the plerocercoids there were found distinct differences in appearance and length of microtriches between these three species, while the microtriches of adult worms were more similar. A regional difference in microtrix appearance was found in the larvae of D. ditremum and D. dendriticum. This was not apparent with S.E.M. in adult worms. The length of ‘body’ microtriches in D. dendriticum varied with the length of the larvae. The topography of the genital atrium of mature and gravid proglottids in adult worms of these three species is also described.     

Loss of Hymenolepis diminuta from the rat

One, 5, 15 and 30 worm infections of Hymenolepis diminuta were established in juvenile or adult male (Hooded Rowett or Sprague-Dawley) rats. Worm numbers and weight, and egg output were determined from day 15 to day 85 post infection. Gradual worm loss occurred only from 15 and 30 worm infections. In 5, 15 and 30 worm infections worm weight decreased from day 19 to day 50 but no weight loss occurred in single worm infections. The size range of individual worms from a multiple infection of a single rat increased markedly following infection. Adult rats showed a greater worm loss and harboured smaller worms than juvenile rats. The data will fit either a competitive or an immunological model.     

Distribution of trichostrongylid nematodes in the abomasum of sheep

The distributions of Haemonchus contortus, Ostertagia circumcincta and Trichostrongylus axei during their development in lambs were studied by freezing and sectioning the abomasum and then recovering the worms from the contents and mucosa of each section. In previously worm-free lambs, the distribution of fourth stage H. contortus was very regular, the frequency of larvae being highest in the anterior fundic area and then decreasing in each section towards the pylorus. The distributions of the early larval stages of O. circumcincta and T. axei were variable but tended to be biomodal, with one peak of larvae in the fundic area and a second in the pyloric area. It is suggested that the difference in distribution between H. contortus and the other two species may be due to a difference in the rate of response of exsheathed third stage larvae to stimuli in the abomasum. In the later stages of development, H. contortus and O. circumcincta tended to migrate towards the pyloric area. In previously infected lambs, the distribution of inhibited early-fourth stage H. contortus was similar to that of normally developing fourth stage larvae in previously worm-free lambs and appeared to be unaffected by the host response which caused an altered distribution and rejection of late-fourth stage and adult worms. The rate of rejection of adult O. circumcincta differed between the fundic and pyloric areas of the abomasum, which suggests that the host response in these two areas may be different.     

Butterfly milkweed extract as a feeding deterrent of the wireworm, Melanotus communis

Crude extracts of butterfly milkweed, Asclepias tuberosa L., were tested for deterrent properties against the corn wireworm, Melanotus communis Gyll (Coleoptera:Elateridae). When offered milkweed-treated and control baits, wireworms fed almost exclusively on untreated food. In no-choice tests, there was a significantly lower frequency of damage to treated baits than control baits. Extended exposure of wireworms to the extract did not affect their survival, feeding vigor, or preference in subsequent feeding tests. Seed corn treated with crude extract exhibited slight phytotoxicity in the form of delayed germination and depressed growth rates.

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Résumé Les propriétés dissuadantes des extraits bruts d'Asclepias tuberosa L. ont été testées contre Melanotus communis Gyll. Quand on a présenté des appâts traités avec A. tuberosa et des appâts témoins, les taupins ont consommé presque exclusivement l'aliment non traité. Dans des expériences sans choix, les appâts traités étaient significativement moins attaqués que les appâts témoins. Une exposition prolongée des taupins à l'extrait n'avait pas modifié leur survie, leur appétit ou leur préférence dans des expériences alimentaires ultérieures. Des graines de maïs traitées avec des extraits bruts ont présenté une légère phytotoxicité sous la forme d'une germination retardée et d'un taux de croissance ralenti.

     

The response of lambs to vaccination at weaning with irradiated Trichostrongylus colubriformis larvae: segregation into 'responders' and 'non-responders'

Dineen J. K., Gregg P. and Lascelles A. K. 1978. The response of lambs to vaccination at weaning with irradiated Trichostrongylus colubriformis larvae: segregation into ‘responders’ and ‘non-responders’. International Journal for Parasitology8: 59–63. Groups of colostrum fed and colostrum deprived lambs were vaccinated with irradiated Trichostrongylus colubriformis larvae at weaning (3 months) and challenged with normal infective larvae. As there was no significant difference between the worm counts of these groups after challenge, it was concluded that the failure of lambs, generally, to respond to vaccination as vigorously as mature sheep, was not due to ‘feed-back inhibition’ of the immune response by the action of maternal antibody.However the results showed that the lambs segregated into ‘responders’ and ‘non-responders’. The mean worm count of responders was 1560 whereas for non-responders this was 24,340. A responder was defined as an animal whose worm count was less than the lower limit of the 99 % confidence interval of unvaccinated controls. These results suggest that genetically-determined factors play an important role in the responsiveness of lambs to vaccination.Whereas counts of globule leucocytes in duodenal tissues were negatively correlated with worm counts (i.e. positively correlated with resistance), counts of both eosinophils and neutrophils were positively correlated with worm counts. These findings suggest that either globule leucocytes are involved in the resistance mechanism, or they are by-products of cellular events involved in resistance. On the other hand it is unlikely that either eosinophils or neutrophils are involved in the mechanism of resistance.     

Fasciola hepatica in rats: Effects of age and infective dose

Groups of 6 male rats, aged either 5, 10, 15 or 25 weeks old, were infected with either 1, 5, 10, 15 or 20 metacercariae of Fasciola hepatica. The prepaient period among all groups ranged from 57 to 60 days, and the size of worms recovered was inconsistent. Within each age group of rats the proportion of metacercariae which developed to maturity was the same irrespective of the dose of metacercariae. These results indicate that neither a crowding effect nor competitive inhibition occurred. Five-week-old rats were generally more susceptible to infection than other age groups. Some variation in the expression of age resistance in 10-week-old animals suggests that it is at about this age that factors involved in age resistance develop.     

Characterization of the nonregular regions of proteins by a contortion index

Nonstructured regions in proteins that provide the link between two regular structured regions play a significant role in maintaining the scaffold of the protein. Not only do they act as connectors between two regular secondary structural elements of proteins but they also provide the necessary turn or reversal in the polypeptide chain. This incorporates flexibility in the structure. Thus an understanding of the structural aspects of the nonregular regions is necessary to have a better insight into these features. We can assume the nonregular region to be a contorted polypeptide segment tethered by regular secondary structured regions at both ends. To describe the undulating nature of the nonregular regions, we introduce a parameter called the "contortion index." This index describes how tortuously the region is organized. Our analysis shows that the contortion index is related to other physicochemical parameters and can be used to characterize the nonregular regions of proteins.     

Cranial anatomy of the extinct amphisbaenian Rhineura hatcherii (Squamata, Amphisbaenia) based on high-resolution X-ray computed tomography

The fossilized skull of a small extinct amphisbaenian referable to Rhineura hatcherii Baur is described from high-resolution X-ray computed tomographic (HRXCT) imagery of a well-preserved mature specimen from the Brule Formation of Badlands National Park, South Dakota. Marked density contrast between bones and surrounding matrix and at bone-to-bone sutures enabled the digital disarticulation of individual skull elements. These novel visualizations provide insight into the otherwise inaccessible three-dimensionally complex structure of the bones of the skull and their relationships to one another, and to the internal cavities and passageways that they enclose. This study corrects several previous misidentifications of elements in the rhineurid skull and sheds light on skull construction generally in "shovel-headed" amphisbaenians. The orbitosphenoids in R. hatcherii are paired and entirely enclosed within the braincase by the frontals; this is in contrast to the condition in many extant amphisbaenians, in which a large azygous orbitosphenoid occupies a topologically distinct area of the skull, closing the anterolateral braincase wall. Rhineura hatcherii retains a vestigial jugal and a partially fused squamosal, both of which are absent in many extant species. Sculpturing on the snout of R. hatcherii represents perforating canals conveying sensory innervation; thus, the face of R. hatcherii receives cutaneous innervation to an unprecedented degree. The HRXCT data (available at www.digimorph.org) corroborate and extend previous hypotheses that the mechanical organization of the head in Rhineura is organized to a large degree around its burrowing lifestyle.     

The influence of high- and low-fibre diets on the activity of piperazine against Oesohagostomum spp. in pigs

An in vivo study evaluated the effect of diet on the efficacy of piperazine against modular worms of pigs. Twenty pigs, later allocated into five groups, were each infected (and 37 days later re-infected) with 3000 infective larvae of a mixed isolate of Oesophagostomum dentatum and Oesophagostomum quadrispinulatum. Beginning on day 23 post infection (p.i.), pigs in groups 1 and 2 were fed a low-fibre diet consisting of 70% barley flour and 30% protein concentrate, while pigs in groups 3, 4 and 5 were fed a high-filure diet consisting of 55% barley flour, 21% oat-husk meal and 24% protein concentrate. On day 42 p.i., pigs in groups 1 and 3 were orally dosed with 200 mg piperazine dihydrochloride (Ascarex D, 53%) per kg bodyweight, the recommended dose, while pigs in group 4 were given 100 mg kg⁻¹. Groups 2 and 5 served as non-treated controls for the respective dictary regimens. Eight days after treatment, the pigs were slaughtered and worms recovered from the caecum and large intestine (divided into five sections) and counted. The mean worn count reduction (WCR) in group 1 (full-dose piperazine with low-fibre diet) was 89.9%, while the high-fibre diet in group 3 increased the WCR to 99.4%. In group 4, where the pigs were fed the high-fibre diet and treated with only 100 mg piperazine kg⁻¹, the WCR was 90.9%, identical to the “low fibre” group 1 treated with twice this piperazine dose. There was a zero efficacy recorded against immature worms in ali three treated groups. The high-fibre diet improved the efficacy of piperazine against more pathogenic and generally more tolerant O. quadrispinulatum to 99.2% compared with 84.3% at the low-fibre diet.