全文获取类型
收费全文 | 1522篇 |
免费 | 86篇 |
国内免费 | 41篇 |
出版年
2023年 | 21篇 |
2022年 | 27篇 |
2021年 | 35篇 |
2020年 | 32篇 |
2019年 | 40篇 |
2018年 | 48篇 |
2017年 | 37篇 |
2016年 | 34篇 |
2015年 | 57篇 |
2014年 | 78篇 |
2013年 | 133篇 |
2012年 | 32篇 |
2011年 | 85篇 |
2010年 | 54篇 |
2009年 | 67篇 |
2008年 | 60篇 |
2007年 | 63篇 |
2006年 | 55篇 |
2005年 | 42篇 |
2004年 | 42篇 |
2003年 | 38篇 |
2002年 | 47篇 |
2001年 | 34篇 |
2000年 | 15篇 |
1999年 | 32篇 |
1998年 | 25篇 |
1997年 | 31篇 |
1996年 | 12篇 |
1995年 | 22篇 |
1994年 | 15篇 |
1993年 | 15篇 |
1992年 | 10篇 |
1991年 | 12篇 |
1990年 | 9篇 |
1989年 | 12篇 |
1988年 | 13篇 |
1987年 | 5篇 |
1985年 | 28篇 |
1984年 | 22篇 |
1983年 | 27篇 |
1982年 | 43篇 |
1981年 | 41篇 |
1980年 | 31篇 |
1979年 | 25篇 |
1978年 | 11篇 |
1977年 | 11篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1974年 | 4篇 |
1973年 | 7篇 |
排序方式: 共有1649条查询结果,搜索用时 250 毫秒
131.
Linda Näsvik Öjemyr Amandine Maréchal Henrik Vestin Brigitte Meunier Peter R. Rich Peter Brzezinski 《BBA》2014
We have studied internal electron transfer during the reaction of Saccharomyces cerevisiae mitochondrial cytochrome c oxidase with dioxygen. Similar absorbance changes were observed with this yeast oxidase as with the previously studied Rhodobacter sphaeroides and bovine mitochondrial oxidases, which suggests that the reaction proceeds along the same trajectory. However, notable differences were observed in rates and electron-transfer equilibrium constants of specific reaction steps, for example the ferryl (F) to oxidized (O) reaction was faster with the yeast (0.4 ms) than with the bovine oxidase (~ 1 ms) and a larger fraction CuA was oxidized with the yeast than with the bovine oxidase in the peroxy (PR) to F reaction. Furthermore, upon replacement of Glu243, located at the end of the so-called D proton pathway, by Asp the PR → F and F → O reactions were slowed by factors of ~ 3 and ~ 10, respectively, and electron transfer from CuA to heme a during the PR → F reaction was not observed. These data indicate that during reduction of dioxygen protons are transferred through the D pathway, via Glu243, to the catalytic site in the yeast mitochondrial oxidase. This article is part of a Special Issue entitled: 18th European Bioenergetic Conference. 相似文献
132.
Minenosuke Matsutani Kota Fukushima Chiho Kayama Misato Arimitsu Hideki Hirakawa Hirohide Toyama Osao Adachi Toshiharu Yakushi Kazunobu Matsushita 《BBA》2014
The bacterial aerobic respiratory chain has a terminal oxidase of the heme-copper oxidase superfamily, comprised of cytochrome c oxidase (COX) and ubiquinol oxidase (UOX); UOX evolved from COX. Acetobacter pasteurianus, an α-Proteobacterial acetic acid bacterium (AAB), produces UOX but not COX, although it has a partial COX gene cluster, ctaBD and ctaA, in addition to the UOX operon cyaBACD. We expressed ctaB and ctaA genes of A. pasteurianus in Escherichia coli and demonstrated their function as heme O and heme A synthases. We also found that the absence of ctaD function is likely due to accumulated mutations. These COX genes are closely related to other α-Proteobacterial COX proteins. However, the UOX operons of AAB are closely related to those of the β/γ-Proteobacteria (γ-type UOX), distinct from the α/β-Proteobacterial proteins (α-type UOX), but different from the other γ-type UOX proteins by the absence of the cyoE heme O synthase. Thus, we suggest that A. pasteurianus has a functional γ-type UOX but has lost the COX genes, with the exception of ctaB and ctaA, which supply the heme O and A moieties for UOX. Our results suggest that, in AAB, COX was replaced by β/γ-Proteobacterial UOX via horizontal gene transfer, while the COX genes, except for the heme O/A synthase genes, were lost. 相似文献
133.
Shuxia Peng Ke ZhouWenjia Wang Zengqiang GaoYuhui Dong Quansheng Liu 《Biochemical and biophysical research communications》2014
Swt1 is an RNA endonuclease that plays an important role in quality control of nuclear messenger ribonucleoprotein particles (mRNPs) in eukaryotes; however, its structural details remain to be elucidated. Here, we report the crystal structure of the C-terminal (CT) domain of Swt1 from Saccharomyces cerevisiae, which shares common characteristics of higher eukaryotes and prokaryotes nucleotide binding (HEPN) domain superfamily. To study in detail the full-length protein structure, we analyzed the low-resolution architecture of Swt1 in solution using small angle X-ray scattering (SAXS) method. Both the CT domain and middle domain exhibited a good fit upon superimposing onto the molecular envelope of Swt1. Our study provides the necessary structural information for detailed analysis of the functional role of Swt1, and its importance in the process of nuclear mRNP surveillance. 相似文献
134.
135.
The aerial parts of Symphyopappus compressus afrorded three new kolavane derivatives and those of S. reticulatus ten diterpenes of this type, two of them esterified with long chain fatty acids. Furthermore, a dihydroxyphenylpropanol, also esterified with long chain fatty acids, was present in both species. The roots of S. reticulatus afforded a new tremetone derivative. The structures were elucidated by spectroscopic methods and some chemical transformations. 相似文献
136.
The structure of sinaicinone, isolated from the aerial parts of the Egyptian medicinal plant Hypericum sinaicum, has been elucidated by means of spectroscopic data such as UV, IR, MS, 1D and 2D NMR spectra, and chemical degradation. It is a complex adamantanyl derivative with a unique skeleton and oxygenated side chains. 相似文献
137.
Koseki M Hirano K Masuda D Ikegami C Tanaka M Ota A Sandoval JC Nakagawa-Toyama Y Sato SB Kobayashi T Shimada Y Ohno-Iwashita Y Matsuura F Shimomura I Yamashita S 《Journal of lipid research》2007,48(2):299-306
Lipid rafts on the cell surface are believed to be very important as platforms for various cellular functions. The aim of this study was to know whether defective lipid efflux may influence lipid rafts on the cell surface and their related cellular functions. We investigated macrophages with defective lipid efflux from ATP binding cassette transporter A1-deficient (Abca1-KO) mice. Lipid rafts were evaluated by the following two novel probes: a biotinylated and protease (subtilisin Carlsberg)-nicked derivative of theta-toxin and a fluorescein ester of polyethylene glycol-derived cholesterol. Lipid rafts in Abca1-KO macrophages were increased, as demonstrated by both probes. Moreover, activities of nuclear factor kappaB, mRNA and intracellular distribution, and secretion of tumor necrosis factor-alpha (TNF-alpha) were examined after stimulation by lipopolysaccharides (LPSs). LPS-induced responses of the activation of nuclear factor kappaB and TNF-alpha were more prompt and accelerated in the Abca1-KO macrophages compared with wild-type macrophages. Modification of lipid rafts by cyclodextrin and nystatin corrected the abnormal response, suggesting an association between the increased lipid rafts and abnormal TNF-alpha secretion. We report here that Abca1-KO macrophages with defective lipid efflux exhibited increased lipid rafts on the cell surface and accelerated TNF-alpha secretion. 相似文献
138.
Santos-Silva T Dias JM Dolla A Durand MC Gonçalves LL Lampreia J Moura I Romão MJ 《Journal of molecular biology》2007,370(4):659-673
Sulphate-reducing bacteria have a wide variety of periplasmic cytochromes involved in electron transfer from the periplasm to the cytoplasm. HmcA is a high molecular mass cytochrome of 550 amino acid residues that harbours 16 c-type heme groups. We report the crystal structure of HmcA isolated from the periplasm of Desulfovibrio gigas. Crystals were grown using polyethylene glycol 8K and zinc acetate, and diffracted beyond 2.1 A resolution. A multiple-wavelength anomalous dispersion experiment at the iron absorption edge enabled us to obtain good-quality phases for structure solution and model building. DgHmcA has a V-shape architecture, already observed in HmcA isolated from Desulfovibrio vulgaris Hildenborough. The presence of an oligosaccharide molecule covalently bound to an Asn residue was observed in the electron density maps of DgHmcA and confirmed by mass spectrometry. Three modified monosaccharides appear at the highly hydrophobic vertex, possibly acting as an anchor of the protein to the cytoplasmic membrane. 相似文献
139.
Dalal A. Al-Mutairi James D. Craik Ines Batinic-Haberle Ludmil T. Benov 《Biochimica et Biophysica Acta (BBA)/General Subjects》2007
Cell proliferation is notably dependent on energy supply and generation of reducing equivalents in the form of NADPH for reductive biosynthesis. Blockage of pathways generating energy and reducing equivalents has proved successful for cancer treatment. We have previously reported that isomeric Zn(II) N-methylpyridylporphyrins (ZnTM-2(3,4)-PyP4+) can act as photosensitizers, preventing cell proliferation and causing cell death in vitro. The present study demonstrates that upon illumination, ZnTM-3-PyP inactivates glucose-6-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase, NADP+ -linked isocitrate dehydrogenase, aconitase, and fumarase in adenocarcinoma LS174T cells. ZnTM-3-PyP4+ was significantly more effective than hematoporphyrin derivative (HpD) for inactivation of all enzymes, except aconitase and isocitrate dehydrogenase. Enzyme inactivation was accompanied by aggregation, presumably due to protein cross-linking of some of the enzymes tested. Inactivation of metabolic enzymes caused disruption of cancer cells' metabolism and is likely to be one of the major reasons for antiproliferative activity of ZnTM-3-PyP. 相似文献
140.
Andrey Karyakin Domantas Motiejunas Rebecca C. Wade Christiane Jung 《Biochimica et Biophysica Acta (BBA)/General Subjects》2007
Recently we have developed a new approach to study protein–protein interactions using Fourier transform infrared spectroscopy in combination with titration experiments and principal component analysis (FTIR-TPCA). In the present paper we review the FTIR-TPCA results obtained for the interaction between cytochrome P450 and the redox partner protein in two P450 systems, the Pseudomonas putida P450cam (CYP101) with putidaredoxin (P450cam–Pdx), and the Bacillus megaterium P450BM-3 (CYP102) heme domain with the FMN domain (P450BMP–FMND). Both P450 systems reveal similarities in the structural changes that occur upon redox partner complex formation. These involve an increase in β-sheets and α-helix content, a decrease in the population of random coil/310-helix structure, a redistribution of turn structures within the interacting proteins and changes in the protonation states or hydrogen-bonding of amino acid carboxylic side chains. We discuss in detail the P450cam–Pdx interaction in comparison with literature data and conclusions drawn from experiments obtained by other spectroscopic techniques. The results are also interpreted in the context of a 3D structural model of the Pdx–P450cam complex. 相似文献