Endothelium-derived stromal cells contribute to hematopoietic bone marrow niche formation

1. Download : Download high-res image (146KB)
2. Download : Download full-size image

     

EZH1 repression generates mature iPSC-derived CAR T cells with enhanced antitumor activity

1. Download : Download high-res image (136KB)
2. Download : Download full-size image

     

Reconstitution of water-oxidizing complex in manganese-depleted photosystem 2 preparations with synthetic manganese complexes

Four synthetic manganese complexes in which Mn atoms have different coordination environments and valence states were used to reconstitute water-oxidizing complex (WOC) in Mn-depleted photosystem 2 preparations. Three Mn-complexes restored a significant rate of electron transfer and oxygen evolution except one complex in which Mn atom ligated to the O-atoms within the ligands by covalent linkage. The effect of coordination environment of the Mn-atom within the Mn-complexes on their efficiencies in reconstituting the electron transport and oxygen evolution was analysed.     

Epigenetic memory of coronavirus infection in innate immune cells and their progenitors

1. Download : Download high-res image (346KB)
2. Download : Download full-size image

     

Dipeptidylpeptidase 4 promotes survival and stemness of acute myeloid leukemia stem cells

1. Download : Download high-res image (93KB)
2. Download : Download full-size image

     

Harnessing matrix stiffness to engineer a bone marrow niche for hematopoietic stem cell rejuvenation

1. Download : Download high-res image (238KB)
2. Download : Download full-size image

     

Lamin B1 deletion in myeloid neoplasms causes nuclear anomaly and altered hematopoietic stem cell function

1. Download : Download high-res image (150KB)
2. Download : Download full-size image

     

Interconverting Gating Modes of a Nonselective Cation Channel from the Tapeworm Echinococcus granulosus Reconstituted on Planar Lipid Bilayers

A 107-pS (symmetrical 150 mm KCl), nonselective cation channel was reconstituted from a microsomal membrane fraction of the larval stage of the tapeworm Echinococcus granulosus. Most of the time, it displayed a high open probability (>0.95) irrespective of either the applied voltage, Ca²⁺, Ba²⁺, or tetraethylammonium concentration. Nevertheless, in contrast with this ``leaklike' behavior, less frequently this ``all-the-time-open' channel reversibly entered two different kinetic modes. One of them was characterized by lower P _o values and some voltage sensitivity (V _?≅ 129 mV, and an equilibrium constant for channel closing changing e-fold per 63-mV change) the kinetic analysis revealing that it resulted from the appearance of voltage-sensitivity in the mean closed times and a sixfold increase in the equilibrium constant for channel closing at 0 mV. The other mode was characterized by a very fast open-close activity leading to poorly resolved current levels and a P _o around 0.6–0.7 which, occasionally and in a voltage-sensitive manner, entered a long-lived nonconducting state. However, the rare nature of these mode-shifting transitions precluded a more detailed analysis of their kinetics. The conductive properties of the channel were not affected by these switches. Model gating alone does not seem to ensure any physiological role of this channel and, instead, some other channel changes must occur if this phenomenon were to be of regulatory importance in vivo. Thus, mode-shifting might constitute an alternative target for channel activity modulation also in tapeworms. Received: 30 August 1996/Revised: 31 January 1997     

T-cell gene therapy

In the past year, a number of human gene therapy trials involving the adoptive transfer of genetically modified T lymphocytes have been reported. These include trials of adenosine deaminase gene transfer in children with severe combined immunodeficiency syndrome, a gene-marking study of Epstein—Barr virus-specific cytotoxic T cells, and trials of gene-modified T cells expressing suicide or viral resistance genes in patients infected with HIV. Additional strategies for T-cell gene therapy currently being pursued in the clinic involve the engineering of novel T-cell receptors that impart antigen specificity for virally infected or malignant cells.     

Aspartate availability limits hematopoietic stem cell function during hematopoietic regeneration

1. Download : Download high-res image (124KB)
2. Download : Download full-size image