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81.
HMG proteins are abundant chromosomal non-histone proteins. It has been suggested that the HMG proteins may play an important
role in the structure and function of chromatin. In the present study, the binding of HMG proteins (HMG1/2 and HMG14/17) to
the core DNA sequence of DNaseI hypersensitive site 2 (HS2core DNA sequence, -10681-10970 bp) in the locus control region
(LCR) of the human β-like globin gene cluster has been examined by using both thein vitro nucleosome reconstitution and the gel mobility shift assays. Here we show that HMG1/2 can bind to the naked HS2core DNA sequence,
however, HMG14/17 cannot. Using thein vitro nucleosome reconstitution we demonstrate that HMG14/17 can bind to the HS2core DNA sequence which is assembled into nucleosomes
with the core histone octamer transferred from chicken erythrocytes. In contrast, HMG1/2 cannot bind to the nucleosomes reconstitutedin vitro with the HS2core DNA sequence. These results indicate that the binding patterns between HMG proteins and the HS2core DNA
sequence which exists in different states (the naked DNA or thein vitro reconstituted nucleosomal DNA) are quite different. We speculate that HMG proteins might play a critical role in the regulation
of the human β-like globin gene’s expression. 相似文献
82.
The typical eukaryote interphase nuclei were reconstructed in vitro following incubation of chromosomes of primitive eukaryote dinoflagellate Crypthecodinium cohnii E. in extracts of S phase eggs of Xenopus laevis L. Micrococcal nuclease digestion experiments indicated that the reconstructed nuclei contained typical nucleosomes. Since the chromosomes of C. cohnii do not contain histone and nucleosomes, these data suggest that nucleosome assembly is independent of specific DNA sequences, nuclear membranes and Lamin. Moreover, it has been demonstrated that Topoisomerase Ⅱ was partially involved in the process of nucleosome assembly. Taken together, these findings suggest that nuclear assembly is independent of formation of nucleosomes and that histone and non-histone may be the decisive factors in this process. 相似文献
83.
Changes in hemocytes of Plutella xylostella after parasitism by Diadegma semiclausum 总被引:1,自引:0,他引:1
Huang F Shi M Yang YY Li JY Chen XX 《Archives of insect biochemistry and physiology》2009,70(3):177-187
We examined the changes of hemocytes in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae), due to parasitism by the endoparasitoid Diadegma semiclausum (Hymenoptera: Ichneumonidae). Necrosis of prohemocytes in different stages was observed while cell death was absent in the mature hemocytes in the parasitized larvae, which was related to the declined total hemocyte count per microliter (THC). THC in the host hemolymph declined sharply by 12 h post-parasitization and then remained at a low level. When hemocytes of the parasitized larvae were cultured in vitro, encapsulation ability was suppressed coincidently with the inhibited spreading ability; however, such effects were transient. Simultaneously, activation of the prophenoloxidae from the hemocytes was inhibited. Unlike the results of previous studies, the decrease in hemocytes, which was due to the necrosis of the prohemocytes instead of the mature hemocytes in our study, was not responsible for the impaired encapsulation. Our studies suggest that parasitism by D. semiclausum have some effects on hematopoietic regulation and on hemocyte immune reaction of P. xylostella larvae. 相似文献
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Jianing Fu Julien Zuber Mercedes Martinez Brittany Shonts Aleksandar Obradovic Hui Wang Sai-ping Lau Amy Xia Elizabeth E. Waffarn Kristjana Frangaj Thomas M. Savage Michael T. Simpson Suxiao Yang Xinzheng V. Guo Michelle Miron Takashi Senda Kortney Rogers Adeeb Rahman Megan Sykes 《Cell Stem Cell》2019,24(2):227-239.e8
87.
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Ex vivo differentiation of umbilical cord blood progenitor cells in the presence of placental conditioned medium 总被引:2,自引:1,他引:1
Chivu M Diaconu CC Brasoveanu L Alexiu I Bleotu C Banceanu G Miscalencu D Cernescu C 《Journal of cellular and molecular medicine》2002,6(4):609-620
Hematopoetic stem cells (HSC) are the progenitors for the lympho-hematopoietic system, with long lifespan and high proliferation potential. Transplantation of HSC from bone marrow or peripheral blood represents a standard therapy in severe hematological conditions. A possible alternative source of HSC is the umbilical cord blood, prepared by various separation procedures followed by expansion in cultures supplemented with hematopoietic growth factors. In order to check the effects of placental conditioned medium (PCM) from placental cells culture upon viability of HSC, we added plasma, PCM, dimetil sulfoxyde or hemin in HSC cultures. Flow cytometry or direct scoring of solid cultures using CD45+, CD34+, CD71+ and CD14+ fluorescent-labeled monoclonal antibodies evaluated the effects upon cell proliferation and colony forming ability of HSC cultures, versus controls. PCM produced the highest proliferation, followed by plasma, DMSO and hemin. PCM improved the survival time and maintained a higher proportion of immature cells. PCM stimulates the differentiation towards myeloid lineage progenitor cells (>90% being CD45+), increasing the percentage of CD14+, granulocites /monocytes precursors. It is highly suggestive that PCM contains growth factors or cytokines, which regulate the development of HSC. Characterization of these factors is in progress. 相似文献
90.
Casein kinase II is composed of two catalytic (a) and two regulatory () subunits, the amino acid sequences of the and subunits are highly conserved between species. To examine whether heterologous casein kinase II could be formed, recombinant and subunits from human andDrosophila were reconstituted from inclusion bodies. Casein kinase II containing either human andDrosophila orDrosophila and human subunits exhibited enzymatic properties similar to those of the homologous holoenzymes with regard to specific activity, salt optima, and autophosphorylation. However, renaturation and reconstitution of casein kinase II was dependent on the type of subunits and the redox conditions, with theDrosophila subunits requiring more reduced conditions. Chimeric subunits prepared from human andDrosophila cDNA revealed that the N-terminal region was responsible for the requirement for the reduced redox state during renaturation. TheN-terminal region also affected solubility and electrophoretic mobility of the subunit. 相似文献