全文获取类型
收费全文 | 51354篇 |
免费 | 18029篇 |
国内免费 | 110篇 |
专业分类
69493篇 |
出版年
2024年 | 17篇 |
2023年 | 202篇 |
2022年 | 211篇 |
2021年 | 737篇 |
2020年 | 3111篇 |
2019年 | 4657篇 |
2018年 | 4912篇 |
2017年 | 4806篇 |
2016年 | 4503篇 |
2015年 | 4434篇 |
2014年 | 4426篇 |
2013年 | 4898篇 |
2012年 | 4037篇 |
2011年 | 4281篇 |
2010年 | 3719篇 |
2009年 | 2706篇 |
2008年 | 2905篇 |
2007年 | 2268篇 |
2006年 | 2314篇 |
2005年 | 1941篇 |
2004年 | 1605篇 |
2003年 | 1678篇 |
2002年 | 1414篇 |
2001年 | 1018篇 |
2000年 | 583篇 |
1999年 | 441篇 |
1998年 | 173篇 |
1997年 | 187篇 |
1996年 | 160篇 |
1995年 | 134篇 |
1994年 | 123篇 |
1993年 | 112篇 |
1992年 | 114篇 |
1991年 | 92篇 |
1990年 | 66篇 |
1989年 | 78篇 |
1988年 | 66篇 |
1987年 | 57篇 |
1986年 | 42篇 |
1985年 | 58篇 |
1984年 | 52篇 |
1983年 | 24篇 |
1982年 | 41篇 |
1981年 | 23篇 |
1980年 | 20篇 |
1979年 | 21篇 |
1978年 | 4篇 |
1977年 | 7篇 |
1976年 | 6篇 |
1973年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
992.
993.
994.
995.
996.
997.
Jonathan Hurtado Dhiraj Acharya Huafang Lai Haiyan Sun Somanath Kallolimath Herta Steinkellner Fengwei Bai Qiang Chen 《Plant biotechnology journal》2020,18(1):266-273
Chikungunya virus (CHIKV) is a mosquito‐transmitted alphavirus, and its infection can cause long‐term debilitating arthritis in humans. Currently, there are no licensed vaccines or therapeutics for human use to combat CHIKV infections. In this study, we explored the feasibility of using an anti‐CHIKV monoclonal antibody (mAb) produced in wild‐type (WT) and glycoengineered (?XFT) Nicotiana benthamiana plants in treating CHIKV infection in a mouse model. CHIKV mAb was efficiently expressed and assembled in plant leaves and enriched to homogeneity by a simple purification scheme. While mAb produced in ?XFT carried a single N‐glycan species at the Fc domain, namely GnGn structures, WT produced mAb exhibited a mixture of N‐glycans including the typical plant GnGnXF3 glycans, accompanied by incompletely processed and oligomannosidic structures. Both WT and ?XFT plant‐produced mAbs demonstrated potent in vitro neutralization activity against CHIKV. Notably, both mAb glycoforms showed in vivo efficacy in a mouse model, with a slight increased efficacy by the ?XFT‐produced mAbs. This is the first report of the efficacy of plant‐produced mAbs against CHIKV, which demonstrates the ability of using plants as an effective platform for production of functionally active CHIKV mAbs and implies optimization of in vivo activity by controlling Fc glycosylation. 相似文献
998.
999.
Xiaohui Yang Yu Yang Jian Ling Jiantao Guan Xiao Guo Daofeng Dong Liping Jin Sanwen Huang Jun Liu Guangcun Li 《Plant biotechnology journal》2020,18(2):364-372
Traditional approaches for sequencing insertion ends of bacterial artificial chromosome (BAC) libraries are laborious and expensive, which are currently some of the bottlenecks limiting a better understanding of the genomic features of auto‐ or allopolyploid species. Here, we developed a highly efficient and low‐cost BAC end analysis protocol, named BAC‐anchor, to identify paired‐end reads containing large internal gaps. Our approach mainly focused on the identification of high‐throughput sequencing reads carrying restriction enzyme cutting sites and searching for large internal gaps based on the mapping locations of both ends of the reads. We sequenced and analysed eight libraries containing over 3 200 000 BAC end clones derived from the BAC library of the tetraploid potato cultivar C88 digested with two restriction enzymes, Cla I and Mlu I. About 25% of the BAC end reads carrying cutting sites generated a 60–100 kb internal gap in the potato DM reference genome, which was consistent with the mapping results of Sanger sequencing of the BAC end clones and indicated large differences between autotetraploid and haploid genotypes in potato. A total of 5341 Cla I‐ and 165 Mlu I‐derived unique reads were distributed on different chromosomes of the DM reference genome and could be used to establish a physical map of target regions and assemble the C88 genome. The reads that matched different chromosomes are especially significant for the further assembly of complex polyploid genomes. Our study provides an example of analysing high‐coverage BAC end libraries with low sequencing cost and is a resource for further genome sequencing studies. 相似文献