全文获取类型
收费全文 | 2657篇 |
免费 | 139篇 |
国内免费 | 628篇 |
出版年
2024年 | 11篇 |
2023年 | 45篇 |
2022年 | 70篇 |
2021年 | 64篇 |
2020年 | 109篇 |
2019年 | 143篇 |
2018年 | 147篇 |
2017年 | 136篇 |
2016年 | 147篇 |
2015年 | 140篇 |
2014年 | 142篇 |
2013年 | 210篇 |
2012年 | 131篇 |
2011年 | 129篇 |
2010年 | 100篇 |
2009年 | 151篇 |
2008年 | 150篇 |
2007年 | 163篇 |
2006年 | 132篇 |
2005年 | 124篇 |
2004年 | 92篇 |
2003年 | 117篇 |
2002年 | 102篇 |
2001年 | 81篇 |
2000年 | 60篇 |
1999年 | 55篇 |
1998年 | 40篇 |
1997年 | 54篇 |
1996年 | 33篇 |
1995年 | 34篇 |
1994年 | 31篇 |
1993年 | 37篇 |
1992年 | 28篇 |
1991年 | 20篇 |
1990年 | 28篇 |
1989年 | 25篇 |
1988年 | 15篇 |
1987年 | 15篇 |
1986年 | 8篇 |
1985年 | 9篇 |
1984年 | 17篇 |
1983年 | 9篇 |
1982年 | 29篇 |
1981年 | 5篇 |
1980年 | 6篇 |
1979年 | 5篇 |
1978年 | 4篇 |
1977年 | 6篇 |
1976年 | 4篇 |
1973年 | 4篇 |
排序方式: 共有3424条查询结果,搜索用时 15 毫秒
21.
Ingemar Renberg 《Hydrobiologia》1986,143(1):379-385
The first part of the paper discusses the significance of using either concentration or accumulation values for expressing the results of investigations of lake sediment cores aimed at studying the history of heavy metal pollution. Neither the values for heavy metal concentration in the lake sediment, whether expressed per gram dry sediment or per gram soluble (organic) sediment, nor the values for their total annual accumulation per unit area of the lake bottom, can, on their own, provide an accurate picture of past pollution conditions, but when considered in combination they render a fairly reliable and detailed interpretation. The second part of the paper deals with Pb, Cd and Hg analyses of cores of varved sediments from several lakes in N. Sweden. Pb and Cd pollution increased during the second half of the 19th century. In most lakes, Hg pollution seems first to have started during the 20th century. Marked increases in both concentration and accumulation of heavy metals took place during the 20th century. For recent decades, the estimated accumulation rates of heavy metals from anthropogenic sources are: Pb 0.5–1.5 µg cm–2 yr–1, Cd 15–30 ng cm–2 yr–1 and Hg 1–2 ng cm–2 yr–1, Higher values were recorded in lakes affected by local emissions. 相似文献
22.
23.
The temperature-dependence of local melting within the subfragment-2 region of rabbit skeletal muscle myosin has been investigated using an enzyme-probe technique. Rate constants of fragmentation of two long subfragment-2 particles (61,000 Mr and 53,000 Mr per polypeptide chain) and a short subfragment-2 particle (34,000 Mr per polypeptide chain) by three different enzymes (alpha-chymotrypsin, trypsin and papain) have been determined over the temperature range 5 to 40 degrees C. We followed the time-course of digestion at specific sites at high (I = 0.50, pH 7.3) and low (physiological, I = 0.15, pH 7.3) ionic strengths by electrophoresis of the digestion products on sodium dodecyl sulfate-containing gels. All rate constants were corrected for the intrinsic temperature-dependence of the enzymes by comparison with model substrates. Normalized rate constant versus temperature profiles for the three enzyme-probes are similar in showing that local melting in long subfragment-2 (61,000 Mr) occurs in two distinct stages as was observed earlier for the intact myosin rod. Over the temperature range 5 to 25 degrees C a restricted region at Mr = 53,000 to 50,000 from the N terminus of the rod (the light meromyosin/heavy meromyosin junction) shows the highest susceptibility to proteolytic cleavage. At temperatures above 25 degrees C local melting was detected by all three enzymes at several specific sites within the hinge domain (Mr = 53,000 to 34,000). Activation energies for cleavage at the susceptible sites were similar for the three enzyme probes. They suggest that this region of the myosin rod has significantly lower thermal stability than the flanking light meromyosin and short subfragment-2 segments. These results, together with other physico-chemical studies, point to the hinge domain of the myosin cross-bridge as an important functional element in the mechanism of force generation in muscle. 相似文献
24.
W D McCubbin G E Willick C M Kay 《Biochemical and biophysical research communications》1973,50(3):926-933
The fluorescent analog of adenosine triphosphate (ATP)1 1,N6-ethenoadenosine triphosphate, (εATP), has been utilized as a substitute for ATP in the myosin and heavy meromyosin ATPase systems. For myosin, the analog εATP replaced ATP with a somewhat larger Km (2.6 × 10?4 mole ??1 for εATP as opposed to 8.8 × 10?5 mole ??1 for ATP), indicating that the apparent affinity of the enzyme for εATP is less than for ATP. Perhaps of more interest, further comparison yielded a Vmax for εATP about two and one half times the value for ATP (20 μmole PO4 sec?1 g protein?1 as opposed to 8.1 μmole sec?1 g protein?1). Results for the HMM-εATPase system were similar, yielding a Km value of 1.47 × 10?4 mole ??1 and a Vmax of 54.2 μmole PO4 sec?1 g protein?1, as opposed to corresponding Km and Vmax values of 1.23 × 10?4 mole ??1 and 20.4 μmole PO4 sec?1 g protein?1, respectively for the HMM-ATP interaction. The pH dependence of εATPase for both systems was comparable to ATP, suggesting a similarity in the mechanism of hydrolysis of the two nucleotides. Activation of εATPase by Ca2+ in the presence of 0.5 M KCl was comparable to ATPase for both systems, but inhibition by Mg2+ seemed to be more effective for εATPase. These results indicate that εATP is an excellent substitute for ATP in the myosin and heavy meromyosin systems and because of its insertion into the active site of these muscle proteins, it promises to be a very useful probe for conformation studies at this level. 相似文献
25.
Shifts in macrophyte species composition as a result of eutrophication and pollution in Dutch transboundary streams over the past decades 总被引:1,自引:0,他引:1
Carleen M. L. Mesters 《Journal of Aquatic Ecosystem Stress and Recovery (Formerly Journal of Aquatic Ecosystem Health)》1995,4(4):295-305
The catchment areas of transboundary streams in the Netherlands have been subject to increasing agricultural and industrial activities over the past decades. To evaluate the effects of these activities on the aquatic vegetation, a study has been carried out in 28 Dutch transboundary lowland streams. Recent data on distribution of 58 aquatic plant species and their growth forms were compared with historical data and were correlated with abiotic variables. Most of these streams lost species that are characteristic for streams and are sensitive to turbidity, eutrophication and pollution (e.g. Potamogeton alpinus, P. polygonifolius, P. densus, Ranunculus peltatus ssp. heterophyllus, Callitriche stagnalis and Myriophyllum alterniflorum.) Species, not common in streams but tolerant to turbidity, eutrophication or pollution (e.g. Potamogeton trichoides, Elodea nuttallii) appeared in many streams or increased in abundance. There was also a shift in growth forms: submerged species decreased or were replaced by emergent/floating-leaved species. Correspondence analysis was carried out to study the relation between the observed changes and the abiotic characteristic of the streams. The magnitude of the shift in species composition was positively correlated with the PO4
3- concentration and pH (which was highly correlated with Cd2+) of the water. This leads to the hypothesis that increased input of sewage, agricultural and industrial water causes a change in species composition and main growth forms of aquatic plant species in lowland streams. 相似文献
26.
The extent of contamination of soils by toxic heavy metals not only depends on the rate of loading of the metal but also on the nature of the adsorbing surfaces, the degree of alkalinity or acidity of the soil and the presence of aqueous complexant ligands. This work reports on the role of pH on the retention of Cd, Hg, Pb and Zn by two soils and on the influence of the chloride, Cl‐, ion on the chemical speciation and retention of the four metals. Batch adsorption experiments were conducted from pH 3 to 7 in the presence of either 0.1 M LiCl or LiClO4. The results of the study showed that high concentrations of Cl‐ ions can greatly decrease the retention of Hg and have an increasingly lesser effect on Cd, Pb and Zn retention. The effect of the Cl‐ons was directly related to the metal‐Cl formation constants. The results of computer modeling of Cd and Hg retention by goethite and humic acid fractions indicated the relative importance of aqueous vs. surface complexation on metal retention. For organic surfaces, which do not form ternary surface complexes, the presence of aqueous complexant ligands should always decrease the adsorption of the metal. For mineral surfaces, which do form ternary surface complexes, there may be increased or decreased metal retention depending on the formation constant of the aqueous metal‐ligand species, the intrinsic complexation constants for the various binary and ternary complexes of the metal and the concentration of the complexant ligand. Thus for Hg, which forms very strong aqueous species with Cl‐ ions, reduced adsorption on goethite was predicted in the presence of 0.1 M LiCl, while enhanced adsorption was predicted for Cd and Pb. The results suggest caution in the disposal of Cl‐containing wastes onto metal‐contaminated soils. The deleterious effects of Cl‐ ion addition would be greatest for soils with relatively high organic matter contents and low contents of hydrous ferric oxides. 相似文献
27.
Michael S. Mooring William Mazhowu Cheryl A. Scott 《Experimental & applied acarology》1994,18(9):507-520
The effect of rainfall pattern on tick challenge was investigated at Kyle Recreational Park, Zimbabwe, from 1991 to 1992 using drag and removal plot methods to sample environmental tick density. The abundance of adults and nymphs of the brown ear-tick Rhipicephalus appendiculatus and larvae of the bont tick Amblyomma hebraeum was positively correlated with monthly rainfall, whereas no relationship with rainfall was revealed for larval R. appendiculatus, adults of the red-legged tick R. evertsi, or larvae of the blue tick Boophilus decoloratus. A comparison between 1991 (490 mm rainfall) and the drought year of 1992 (161 mm) revealed significant differences in the abundance of R. appendiculatus, A. hebraeum, and B. decoloratus. During the wet season, R. appendiculatus adults were 2–3 time more numerous in the environment during the higher rainfall year of 1991. A. hebraeum larval abundance exhibited a similar pattern to that of R. appendiculatus adults, but B. decoloratus larvae were more abundant in the drought year of 1992 during both the wet and dry seasons. Comparable tick abundance data collected at Kyle during the above-average rainfall years of 1975–1977 (mean = 1029 mm) were compared with tick challenge during the below-average rainfall years of 1991–1992 (mean = 326 mm). In grassland sand habitat and all habitats combined R. appendiculatus adults, nymphs, and larvae were much more abundant during the high rainfall years. In contrast, larvae of B. decoloratus were more numerous during the drier years. A. hebraeum larvae were also more abundant during the drier years. The strong positive correlation of adult R. appendiculatus abundance with rainfall and the coincidence of increased adult tick challenge with increased rainfall indicates that adult R. appendiculatus tick burden on hosts would be heaviest during the wet season and high rainfall years. 相似文献
28.
R. F. M. van Steveninck A. Babare D. R. Fernando M. E. van Steveninck 《Plant and Soil》1994,167(1):157-164
Plant species adapted to soils enriched with heavy metals often accumulate these metals in their above or below ground organs. In this study, electron probe microanalysis of fractured, quench-frozen root specimens of common crop species shows that an appreciable quantity of Zn can be bound as Zn phytate (myo-inositolkis-hexaphosphate) within small vacuoles of cells in the root elongation zone of lucerne, soybean, lupins, tomato, rapeseed, cabbage, radish, maize and wheat exposed to high levels of Zn (80–300 M). Globular deposits of Zn phytate are most frequently observed in the endodermis of dicotyledonous species and in the pericycle of monocotyledonous species, but may also occur in the stele and inner cortex after prolonged exposure to toxic levels of Zn. The deposits could not be found in Zn-treated sunflower, field peas and Italian ryegrass. In three crop species, lucerne, soybean and maize, Zn-induced phytate globules were frequent, but exposure of roots to 30 M Cd did not induce the formation of Cd-containing globular deposits as observed inLemna minor (Van Steveninck et al., 1990a, 1992). Simultaneous Zn and Cd treatment induced the formation of Zn phytate globules as effectively as Zn alone, and Cd was not detected in the deposits. 相似文献
29.
Comparison of the chemical changes in the rhizosphere of the nickel hyperaccumulator Alyssum murale with the non-accumulator Raphanus sativus 总被引:2,自引:1,他引:1
Changes in pH and redox potential were studied in the rhizosphere soil of a nickel hyperaccumulator plant (Alyssum murale) and of a crop plant, radish (Raphanus sativus). Differences in rhizosphere pH and reducing activity were found between the lateral and the main roots of both species,
but the pH changes in the rhizosphere were similar in both species. Changes in pH were associated with the relative uptakes
of cations and anions; whether the concentrations of heavy metals in the growth medium did not have any effect on the rhizosphere
pH. The source of nitrogen (ammonium or nitrate) was the major factor determining the pH of the rhizosphere of both species.
The redox potential of the rhizosphere was influenced by both the N-source and the concentrations of heavy metals. When heavy
metals were not present in the growth medium, and nitrate was the N-source, the reducing capacity of A. murale roots was enhanced. However, the reducing activity of A. murale was always smaller than that of radish. Therefore, the mechanism of metal solubilization by the hyperaccumulator plant does
not involve either the reduction of pH in the rhizosphere or the release of reductants from roots. The acidification and reducing
activity of the roots of A. murale was always smaller than that of R. sativus. 相似文献
30.
Simultaneous extraction of soil phytoavailable cadmium,copper, and lead by chelating resin membrane 总被引:2,自引:0,他引:2
Cadmium, copper, and lead were extracted from suspensions of contaminated soils using metal chelating exchange resin membranes.
Nine soils with widely varying properties and Cd, Cu and Pb levels were tested. Soil suspensions made up with 4 g in 40 mL
deionized water were equilibrated with 5 cm2 Ca-saturated Chelex exchange resin membrane which was retained inside a polypropylene bag and shaken at 150 rpm for 24 hrs.
Resin membrane extractable Cd, Cu and Pb of the soils were correlated with Cd, Cu, and Pb uptake by young wheat seedlings
grown in these soils and compared with soil Cd, Cu, and Pb extracted by 0.1 M HCl, 0.01 M CaCl2, and 0.005 M Diethylenetriamine pentaacetic acid (DTPA). The amounts of Cd, Cu and Pb extracted by the Ca-saturated Chelex membrane from
all tested soils correlated well with those absorbed by young wheat seedlings. The Ca-saturated Chelex membrane extractable
Cd, Cu and Pb of the soil had the strongest correlation with plant uptake Cd, Cu and Pb among the extraction methods we tested.
It was demonstrated that the Ca-saturated Chelex membrane extraction is an appropriate method in simultaneously estimating
Cd, Cu and Pb phytoavailability of soil and is applicable to a wide range of soils. 相似文献