全文获取类型
收费全文 | 4657篇 |
免费 | 499篇 |
国内免费 | 416篇 |
出版年
2024年 | 10篇 |
2023年 | 96篇 |
2022年 | 78篇 |
2021年 | 137篇 |
2020年 | 195篇 |
2019年 | 221篇 |
2018年 | 208篇 |
2017年 | 180篇 |
2016年 | 185篇 |
2015年 | 221篇 |
2014年 | 284篇 |
2013年 | 336篇 |
2012年 | 229篇 |
2011年 | 239篇 |
2010年 | 203篇 |
2009年 | 235篇 |
2008年 | 255篇 |
2007年 | 211篇 |
2006年 | 228篇 |
2005年 | 183篇 |
2004年 | 169篇 |
2003年 | 148篇 |
2002年 | 146篇 |
2001年 | 152篇 |
2000年 | 117篇 |
1999年 | 85篇 |
1998年 | 116篇 |
1997年 | 85篇 |
1996年 | 76篇 |
1995年 | 72篇 |
1994年 | 60篇 |
1993年 | 62篇 |
1992年 | 48篇 |
1991年 | 32篇 |
1990年 | 34篇 |
1989年 | 22篇 |
1988年 | 17篇 |
1987年 | 30篇 |
1986年 | 20篇 |
1985年 | 19篇 |
1984年 | 22篇 |
1983年 | 18篇 |
1982年 | 25篇 |
1981年 | 12篇 |
1980年 | 9篇 |
1979年 | 17篇 |
1978年 | 5篇 |
1977年 | 7篇 |
1976年 | 6篇 |
1974年 | 3篇 |
排序方式: 共有5572条查询结果,搜索用时 265 毫秒
781.
H. B. Freifeld 《Journal of Biogeography》1999,26(6):1191-1213
Aim Our knowledge of landbirds on tropical Pacific islands is often comprised of brief, one-time surveys. We know little of species’ habitat preferences, and this information is critical for understanding the resource requirements or population status of native species and the impacts of human activity on island birds. Location Tutuila Island, American Samoa. Methods The spatial patterns in forest birds were investigated. This island harbours large tracts of native forest, a variety of disturbed and non-native forests, and some relatively healthy bird populations. The distribution of birds was correlated with forest types using habitat data collected at fifty-seven sites and avian census data collected monthly for 1–4 years at these sites. Results Differences in location and in vegetation structure and composition between native and non-native habitats are important influences on the distribution of birds on Tutuila. Among native species, for example, the purple-capped fruit-dove (Ptilinopus porphyraceus Temminck) is dependent upon native habitat, the Samoan starling (Aplonis atrifusca Peale) occurs in all habitats, and the cardinal honeyeater (Myzomela cardinalis Gmelin) is more abundant in low elevation, non-native habitat. Conclusions This research reinforces the importance of quantitative assessment of habitat relationships in the study and conservation of Pacific birds. 相似文献
782.
Effects of environmental factors on circadian activity in the flesh fly,
Sarcophaga crassipalpis 总被引:1,自引:0,他引:1
Abstract.The diel locomotor activity patterns of wandering larvae in the flesh fly, Sarcophaga crassipalpis Macquart (Diptera: Sarcophagidae), were examined using a novel apparatus and shown to be primarily diurnal, but with a minority (37%) showing nocturnal activity. In response to the environmental stress of heat shock, a significantly larger proportion (72%) of the larvae became nocturnal. In comparison, adult circadian activity also was predominantly diurnal, but not correlated with the larval activity patterns. In addition, adult patterns showed age-related changes in entrainment and free running period. Finally, the phase of circadian-gated adult eclosion was shown to be entrained by a 3-day exposure to light–dark cycles delivered prior to pupariation, with the phase maintained throughout pupal–adult metamorphosis under constant dark conditions. These results demonstrate that environmental changes may have profound effects on the expression of 24-h activity patterns and circadian rhythms during different life stages throughout development. 相似文献
783.
利用优选法和快速估计法对叶色草岭在7个温度处理下的个体发育的过程进行了测算,确定了它的卵期、幼虫期、蛹期及幼虫期的一龄、二龄和三龄的发育起始温度(T_0)和有效积温(K),前一方法的结果T_0分别为14.20、11.55、8.27、10.84、10.03和12.79℃;K分别为53.82、151.58、243.85、52.14、45.86和55.20日/度。后一方法的结果T_0分别为14.33、12.18、8.38、 11.62、10.66和13.28℃;K分别为54.41、157.62、245.21、54.61、47.52和57.04日/度。同时根据Logistic曲线方程确定了不同温度条件下叶色草蛉的发育速率计算式。 相似文献
784.
With the increasingly competitive commercial production of target proteins by hybridoma and genetically engineered cells,
there is an urgent requirement for biosensors to monitor and control on-line and in real time the growth of cultured cells.
Since growth is accompanied by an enthalpy change, heat dissipation measured by calorimetry could act as an index for metabolic
flow rate. Recombinant CHO cell suspensions producing interferon-γ were pumped to an on-line flow calorimeter. The results
showed that an early reflection of metabolic change is size-specific heat flux obtained from dividing heat flow rate by the
capacitance change of the cell suspension, using the on-line probe of a dielectric spectroscope. Comparison of heat flux with
glucose and glutamine fluxes indicated that the former most accurately reflected decreased metabolic activity. Possibly this
was due to accumulation of lactate and ammonia resulting from catabolic substrates being used as biosynthetic precursors.
Thus, the heat flux probe is an ideal on-line biosensor for fed-batch culture. A stoichiometric growth reaction was formulated
and data for material and heat fluxes incorporated into it. This showed that cell demand for glucose and glutamine was in
the stoichiometric ratio of ∼3:1 rather than the ∼5:1 in the medium. It was demonstrated that the set of stoichiometric coefficients
in the reaction were related through the extent of reaction (advancement) to overall metabolic activity (flux). The fact that
this approach can be used for medium optimisation is the basis for an amino-acid-enriched medium which improved cell growth
while decreasing catabolic fluxes.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
785.
Heat capacity has played a prominent role in relating macroscopic and microscopic properties of small molecules and crystals. However, its diagnostic power can also be used for macromolecules such as proteins. It is shown in the present study that the macroscopically observed protein heat capacity provides direct access to the thermodynamic state of the single protein molecule. The new model of the physical basis of protein heat capacity emphasizes the dynamic nature of protein molecules. It incorporates equilibrium fluctuations as an integral constituent and shows that the increase in the magnitude of equilibrium fluctuations is coupled to an increase in the enthalpy flux between the individual protein molecule and its surroundings. Proteins 2000;41:86–92. © 2000 Wiley‐Liss, Inc. 相似文献
786.
787.
Júlio Csar Bicca-Marques Cludia Calegaro-Marques 《American journal of physical anthropology》1998,106(4):533-546
Behavioral thermoregulation in primates may provide a means for the conservation of heat during periods of low ambient temperature and/or food shortage as well as a way to dissipate heat under hot conditions. This article focuses on behavioral thermoregulation in a sexually dichromatic primate, the black-and-gold howling monkey (Alouatta caraya). Two models have been proposed to explain the evolution of sexual dichromatism in this species: thermoregulation and sexual selection. Five hypotheses associated with thermoregulatory behaviors are tested. These are as follows: (1) energy-conserving postures are used mainly under low ambient temperatures; (2) sunny resting places are selected during periods of low temperature; (3) exposure of the less-insulated ventral region to sunlight decreases with increasing temperature; (4) black-colored adult males use energy-conserving postures, sunny places, and exposure of the ventral region to sunlight less frequently than do blonde-colored adult females; and (5) smaller individuals use energy-conserving postures, sunny places, and exposure of the ventral region to sunlight in significantly greater frequency than do larger individuals. Over a 12-month period, behavioral data were collected on a free-ranging habituated group of 15–17 howlers of all age-sex classes. Ambient temperature was measured each hour. The results indicate that during resting, howlers showed a consistent use of heat-conserving postures, showed a preference for sunny places, and exposed their ventral region to sunlight under low ambient temperatures. A preference for shady places, heat-dissipating postures, and exposure of the back were observed under high ambient temperatures. Despite sex differences in adult color patterns and differences in size between age classes, no significant age or sex differences in thermoregulatory behaviors were detected. Failure to confirm a thermoregulation model implies that sexual selection may be responsible for sexual dichromatism in this species. Am J Phys Anthropol 106:533–546, 1998. © 1998 Wiley-Liss, Inc. 相似文献
788.
Enthalpy and heat capacity changes for the deprotonation of 18 buffers were calorimetrically determined in 0.1 M potassium chloride at temperatures ranging from 5 to 45°C. The values of the dissociation constant were also determined by means of potentiometric titration. The enthalpy changes for the deprotonation of buffers, except for the phosphate and glycerol 2-phosphate buffers, were found to be characterized by a linear function of temperature. The enthalpy changes for the second dissociation of phosphate and glycerol 2-phosphate where divalent anion is formed on dissociation were fitted with the second order function of temperature rather than the first order. Temperature dependence of buffer pH calculated by using the enthalpy and heat capacity changes obtained was in good agreement with the temperature variation of the pH values actually measured in the temperature range between 0 and 50°C for all the buffers studied. On the basis of the results obtained, a numeric table showing the temperature dependence of pK values for the 18 buffers is presented. Proteins 33:159–166, 1998. © 1998 Wiley-Liss, Inc. 相似文献
789.
790.