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991.
992.
HIROAKI SHIOTA MASAHITO T. KIMURA 《Biological journal of the Linnean Society. Linnean Society of London》2007,90(2):375-380
Cold-tolerant ectothermal animals are generally absent from warmer regions, suggesting that the acquisition of cold tolerance is associated with the loss of adaptation to warmer environments. In the present study, we compared thermal tolerance, walking speed (WS) and pupal development (PD) for 28 drosophilid species from cool-temperate, warm-temperate, and subtropical regions by the phylogenetically-based method and the conventional regression to understand trade-offs between these traits. A significant negative relationship was observed between cold tolerance and the rate of PD in both sexes and between cold tolerance and WS in the male in the phylogenetically-based analysis. Similar results were obtained in the conventional regression analysis, although the level of significance somewhat differed. Thus, cold tolerant species are assumed to have lost abilities to develop and walk fast. Subsequently, they may become more vulnerable to predators, parasitoids or infectious microorganisms and may have become extinct in warmer regions through enemy-mediated interactions and/or resource competition with cold susceptible species with faster development and quicker locomotion. In the present study, no significant relationship was observed between heat tolerance and WS or the rate of PD in the phylogenetically-based analysis, although heat tolerance was significantly related with the rate of PD in the conventional regression analysis. Thus, trade-offs associated with heat tolerance were not apparent. © 2007 The Linnean Society of London, Biological Journal of the Linnean Society , 2007, 90 , 375–380. 相似文献
993.
There is accumulating evidence that the estrogen receptor (ER) can transduce specific signals at the plasma membrane. We tried to clarify the biological function of ER as a signaling molecule by identifying proteins that interact with the membrane-localized ER. The activation function 1 and 2 (AF-1 and AF-2) domains of ERalpha with or without the membrane-targeting sequence were stably expressed in the breast cancer cell line, MCF-7. The level of tyrosine phosphorylation of AF-2 was significantly elevated by the membrane localization. By mass-spectrometry analysis, alpha- and beta-tubulins and heat shock protein 70 were identified as the AF-1-associated proteins. Of these, tubulins are associated only with membrane-targeted AF-1. 相似文献
994.
Genistein inhibits herbimycin A-induced overexpression of inducible heat shock protein 70 kDa 总被引:1,自引:0,他引:1
Kiang JG 《Molecular and cellular biochemistry》2003,245(1-2):191-199
995.
Agarwal M Sahi C Katiyar-Agarwal S Agarwal S Young T Gallie DR Sharma VM Ganesan K Grover A 《Plant molecular biology》2003,51(4):543-553
996.
Verschuure P Tatard C Boelens WC Grongnet JF David JC 《European journal of cell biology》2003,82(10):523-530
Recently, we have described the developmental expression of the small heat shock proteins (sHsps) Hsp27/HspB1 and alphaB-crystallin/HspB5 in different tissues of pigs from almost full-term foetuses to three years old adults (P. Tallot, J. F. Grongnet, J. C. David, Biol. Neonate, 83, 281-288, 2003). The data described in this report extends this study to four other members of the sHsp family (Hsp20/HspB6, cvHsp/HspB7, MKBP/HspB2 and HspB8). We studied expression of these proteins in porcine lens, brain, heart, liver, kidney, lung, skeletal muscle, stomach, and colon, and found a ubiquitous expression of Hsp20 and HspB8 as earlier reported for Hsp27 and alphaB-crystallin. In contrast, cvHsp and HspB2 expression is essentially restricted to heart and muscle. During development, the sHsps tend to (temporarily) increase in stomach, liver, lung, kidney, hippocampus, and striatum, while expression in heart is more or less constant, and a large variation is found in sHsp expression patterns in skeletal muscle. In cerebellum and cortex a temporary decrease of Hsp20 and HspB8 is observed directly after birth. The major impact of this study is that each tissue seems to have a unique profile of sHsp expression, which varies during development and may reflect the need of a particular tissue to maintain at all stages an optimal chaperoning machinery to protect against physiological stress. 相似文献
997.
Juneja VK 《Letters in applied microbiology》2003,37(4):292-298
AIMS: Thermal inactivation of a mixture of five strains of Listeria monocytogenes, four strains of Escherichia coli O157:H7 and eight serotypes of Salmonella were compared with that of indigenous microflora in 75% lean ground beef. METHODS AND RESULTS: Inoculated meat was packaged in bags that were completely immersed in a circulating water bath and held at 55, 57.5 and 60 degrees C for predetermined lengths of time. The surviving cell population was enumerated by spiral plating heat-treated samples onto tryptic soya agar supplemented with 0.6% yeast extract and 1% sodium pyruvate. D-values, determined by linear regression, in beef were 77.49, 21.9, and 10.66 min at 55, 57.5, and 60 degrees C, respectively, for indigenous microflora (z = 5.81 degrees C). When either of the three pathogens were heated in beef, their D-values calculated were significantly lower (P < 0.05) than those of indigenous microflora at all temperatures. The slope of the thermal death time curve for L. monocytogenes, E. coli O157:H7 and indigenous microflora were similar. Using a survival model for nonlinear survival curves, the D1-values at all temperatures for L. monocytogenes were significantly higher (P < 0.05) compared with those for Salmonella serotypes, E. coli O157:H7 or indigenous microflora. However, higher recovery of a subpopulation of the indigenous microflora in beef exposed to heating at 55, 57.5 or 60 degrees C resulted in significantly higher (P < 0.05) D2-values at all three temperatures, compared with those of the three pathogens at the same test temperatures. CONCLUSIONS: If the thermal process is designed to ensure destruction of indigenous microbial flora, it should also provide an adequate degree of protection against L. monocytogenes, Salmonella serotypes or E. coli O157:H7. SIGNIFICANCE AND IMPACT OF THE STUDY: The results of this study will assist the retail food industry in designing acceptance limits on critical control points that ensure safety, without introducing pathogens in a retail food environment, against L. monocytogenes, E. coli O157:H7 and Salmonella in cooked ground beef. 相似文献
998.
Kiang JG McClain DE Warke VG Krishnan S Tsokos GC 《Journal of cellular biochemistry》2003,89(5):1030-1043
For many types of cells, heat stress leads to an increase in intracellular free calcium concentration ([Ca2+](i)) that has been shown to trigger a wide variety of cellular responses. In T lymphocytes, for example, heat stress stimulates pathways that make them more susceptible to Fas/CD95-mediated apoptosis. Because of our interest in understanding more about the response of lymphocytes to various stressors, we used human peripheral and Jurkat T lymphocytes to investigate the effect of heat stress on calcium homeostasis. We found that peripheral and Jurkat T cells both exhibit cNOs activity but not iNOs activity. Heat stress increased NO production, which was inhibited by LNNA (a cNOs inhibitor) but not L-NIL (an iNOs inhibitor). Heat stress increased [Ca2+](i) in Jurkat T cells by decreasing the K(m) of the cell surface membrane Na+/Ca2+ exchanger for extracellular Ca2+. Heating also increased cNOs phosphorylation at tyrosine residues. In cells incubated with LNNA, heat stress promoted an increase in [Ca2+](i) and a decrease in [Na+](i) greater than in cells heated without LNNA, a larger decrease in K(m) of the Na+/Ca2+ exchanger for extracellular Ca2+, and decreased phosphorylation of cNOs. Our results suggest that cNOs plays an important regulatory role after heat stress. Heating appears to increase the phosphorylation of cNOs that is complexed with the Na+/Ca2+ exchanger to decrease its activity. This process is related to increased expression of Fas/CD95 on the cell surface, which might explain the apoptotic diathesis of lymphocytes after heat stress. 相似文献
999.
We examined the effects of probiotic Lactobacillus strains of Lactobacillus agilis JCM 1048 and Lactobacillus salivarius subsp. salicinius JCM 1230 on jejunal and cecal microbiota of broiler chicken under heat stress condition using terminal restriction fragment length polymorphism (T-RFLP) analysis. The jejunal bacterial community was limited to a few bacterial groups, mostly Lactobacillus spp. A relatively abundant and higher prevalence of Lactobacillus spp. were observed in the jejunal and cecal microbiota of the probiotic chickens compared with those of the control chickens under heat stress condition. In general, the probiotic strains did not significantly affect the abundance of L. agilis and L. salivarius in chicken intestine but clearly contributed to increasing their prevalence in the probiotic chickens. The probiotic Lactobacillus strains enriched the diversity of Lactobacillus flora in chicken jejunum and cecum by increasing the abundance and prevalence of Lactobacillus spp. inhabiting the intestine. The richness of Lactobacillus species tended to be similar among the jejunal and cecal microbiota. The bacterial community of cecum was complex and age-dependent. The major components of the cecal microbiota were clostridia and lactobacilli. The Clostridium subcluster XIVa was the most predominant group in chicken cecum. Probiotic Lactobacillus strains restored the microbial balance and maintained the natural stability of indigenous bacterial microbiota following heat stress-induced changes. 相似文献
1000.
R. S. SEYMOUR 《Plant, cell & environment》2004,27(8):1014-1022
Inflorescences of the arum lily Symplocarpus foetidus are thermogenic and thermoregulatory. The spadix increases respiratory heat production rate as ambient temperature decreases. This study examined the relationships between spadix temperature (Ts), respiration rate () and ambient temperature (Ta) at equilibrium and during transient responses to step changes in Ta. Intact inflorescences inside a miniature constant temperature cabinet in the field showed the most precise temperature regulation yet recorded; over a 37.4 °C range in Ta (?10.3 to 27.1 °C), Ts changed only 3.5 °C (22.7 to 26.2 °C). Regulated temperatures were not related to spadix size (1.9–7.3 g) or circadian cycle. Dynamic responses to step changes in Ta involved a phasic change in Ts, first in the same direction as Ta, then reversing at 38.3 min, and finally approaching equilibrium at 87.6 min, on average. Meanwhile changed in a monotonic curve toward equilibrium. Models revealed that the dynamics of temperature change were inconsistent with simply a physical lag in the system, but involved some form of biochemical regulation, possibly by changes in activity of a rate‐limiting functional protein. 相似文献