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621.
The long-wavelength chlorophyll states of plant LHCI at room temperature: a comparison with PSI-LHCI
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The red antenna states of the external antenna complexes of higher plant photosystem I, known as LHCI, have been analyzed by measurement of their preequilibrium fluorescence upon direct excitation at 280 K. In addition to the previously detected F735 state, a hitherto undetected low-energy state with emission maximum around 713 nm was observed. The 280 K bandwidths (FWHM) are 55 nm for the F735 state and approximately 27 nm for the F713-nm state, much greater than for non-red-shifted antenna chlorophylls. The origin absorption band for the F735-nm state was directly detected by determination of its excitation (action) spectrum and lies at 709-710 nm. The absorption spectrum for F735, calculated using the Stepanov expression, closely overlaps the excitation spectrum, indicating that the very large Stokes shift (25 nm) is due to vibrational relaxation within the excited-state manifold and solvent effects can be excluded. Fluorescence anisotropy measurements, with direct excitation of F735, indicate that the transition dipoles of the two red states are parallel. Similar experiments performed in the long-wavelength absorbing tail of PSI-LHCI indicate the presence of emission state(s) that are red-shifted with respect to F735 of isolated LHCI. It is suggested that these are brought about by interactions between the complexes in PSI-LHCI, which occur in some yet undefined way, and which are broken upon solubilization of the component parts. 相似文献
622.
Cryptomonads and chlorarachniophytes acquired photosynthesis independently by engulfing and retaining eukaryotic algal cells. The nucleus of the engulfed cells (known as a nucleomorph) is much reduced and encodes only a handful of the numerous essential plastid proteins normally encoded by the nucleus of chloroplast-containing organisms. In cryptomonads and chlorarachniophytes these proteins are thought to be encoded by genes in the secondary host nucleus. Genes for these proteins were potentially transferred from the nucleomorph (symbiont nucleus) to the secondary host nucleus; nucleus to nucleus intracellular gene transfers. We isolated complementary DNA clones (cDNAs) for chlorophyll-binding proteins from a cryptomonad and a chlorarachniophyte. In each organism these genes reside in the secondary host nuclei, but phylogenetic evidence, and analysis of the targeting mechanisms, suggest the genes were initially in the respective nucleomorphs (symbiont nuclei). Implications for origins of secondary endosymbiotic algae are discussed. 相似文献
623.
Cells aggregate and can be recovered from suspension when exposed to an ultrasonic standing wave field. The acoustic force on individual cells in a standing wave decreases with particle volume. A plane ultrasonic field generated by a transducer driven at 3.3 MHz was used here to investigate the removal of Escherischia coli, cells with dimensions of the order of 1.0 m, from batch suspension by sedimentation over a range of concentrations (103 to 1010 cells ml–1). Cell removal efficiencies greater than 90% were achieved at initial concentrations of 1010 cells ml–1. Removal efficiencies decreased gradually to zero, as initial bacterial concentration was reduced to 107 cells ml–1. It was found that, when low concentrations of E. coli (103 to 105 cells ml–1) were added to suspensions of larger particles (i.e. yeast cells) that were of sufficient concentration to form aggregates in the sound field, E. coli could be harvested to an efficiency of 40%. The results imply that the E. coli became trapped and sediment with aggregates of larger particles. Some strains of bacteria are capable of DNA transfer by conjugation. The transfer rate of E. coli RP4 plasmid is order of magnitudes greater when conjugation occurs on solid medium rather than in liquid suspension. We have investigated whether the conjugation rate would also be higher in ultrasonically induced E. coli clumps than in free suspension. The donor strain was mixed with a recipient strain of E. coli, then sonicated in a capillary at 4.6 MHz in a tubular transducer for 5 min. The bacteria aggregated successfully. Results showed a three-fold increase in the rate of conjugation compared to a liquid mating control. 相似文献
624.
625.
Ecosystem restoration frequently involves the reintroduction of plant material in the degraded ecosystem. When there are no plant nurseries or seeds available on the market, the plant material has to be harvested in the wild, in a “donor ecosystem.” A comprehensive assessment of donor ecosystem recovery is lacking, especially for Sphagnum‐dominated donor peatlands, where all top vegetation is harvested mechanically with different practices. We aimed to evaluate (1) the regeneration of vegetation, especially of Sphagnum mosses, to determine which harvesting practices are best to enhance recovery and (2) the influence of the site hydrological conditions and meteorological variables of the first complete growing season postharvesting on peat moss regeneration. Twenty‐five donor sites covering a 17‐year chronosequence (harvested 1–17 years ago) were inventoried along with 15 associated natural reference sites located in Quebec, New Brunswick, and Alberta, Canada. All donor sites aged 10 years or more were dominated by Sphagnum mosses, though plant composition varied between donor and their associated reference sites because of the wetter conditions at harvested donor sites. Harvesting practices strongly influenced donor site recovery, showing that the skills of the practitioner are an essential ingredient. Harvesting practices minimizing donor site disturbances are recommended, such as the choice of the adequate donor site (localization, hydrologic conditions, vegetation), the use of less disruptive methods, and harvesting when the soil is deeply frozen. This study demonstrated that harvesting surface plant material for peatland restoration is not detrimental towards the recovery of near‐natural peatland ecosystems. 相似文献
626.
Alexander V. Vener 《BBA》2007,1767(6):449-457
Recent advances in vectorial proteomics of protein domains exposed to the surface of photosynthetic thylakoid membranes of plants and the green alga Chlamydomonas reinhardtii allowed mapping of in vivo phosphorylation sites in integral and peripheral membrane proteins. In plants, significant changes of thylakoid protein phosphorylation are observed in response to stress, particularly in photosystem II under high light or high temperature stress. Thylakoid protein phosphorylation in the algae is much more responsive to the ambient redox and light conditions, as well as to CO2 availability. The light-dependent multiple and differential phosphorylation of CP29 linker protein in the green algae is suggested to control photosynthetic state transitions and uncoupling of light harvesting proteins from photosystem II under high light. The similar role for regulation of the dynamic distribution of light harvesting proteins in plants is proposed for the TSP9 protein, which together with other recently discovered peripheral proteins undergoes specific environment- and redox-dependent phosphorylation at the thylakoid surface. This review focuses on the environmentally modulated reversible phosphorylation of thylakoid proteins related to their membrane dynamics and affinity towards particular photosynthetic protein complexes. 相似文献
627.
Marcel Gueltig Hinnerk Ossmer Makoto Ohtsuka Hiroyuki Miki Koki Tsuchiya Toshiyuki Takagi Manfred Kohl 《Liver Transplantation》2014,4(17)
A new method for thermal energy harvesting at small temperature difference and high cycling frequency is presented that exploits the unique magnetic properties and actuation capability of magnetic shape memory alloy (MSMA) films. Polycrystalline films of the Ni50.4Co3.7Mn32.8In13.1 alloy are tailored, showing a large abrupt change of magnetization and low thermal hysteresis well above room temperature. Based on this material, a free‐standing film device is designed that exhibits thermomagnetically induced actuation between a heat source and sink with short heat transfer times. The cycling frequency of the device is tuned by mechanical frequency up‐conversion to over 200 Hz. An integrated pick‐up coil converts the thermally induced change of magnetization as well as the kinetic energy to electricity. For a temperature change of 10 K, the maximum peak power density is in the order of 5 mW cm‐3. 相似文献
628.
Capacitive Energy Harvesting Using Highly Stretchable Silicone–Carbon Nanotube Composite Electrodes
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Paul Brochu Hristiyan Stoyanov Ryan Chang Xiaofan Niu Wei Hu Qibing Pei 《Liver Transplantation》2014,4(3)
Capacitive energy harvesters utilizing elastic dielectrics offer a simple way to harvest energy from natural mechanical energy sources. While the technology is promising due to its simplicity and low cost combined with high efficiency and energy density, there are still material challenges that must be addressed. For effective energy conversion, the dielectric material should have low dielectric and mechanical losses, while the compliant electrodes should be able to withstand large strains over an extended lifetime without any substantial loss of conductivity. The development of soft flexible and stretchable silicone–carbon nanotube composite electrodes is presented for use in capacitive energy harvesting and strain sensing. The composite is capable of being stretched to over 150% strain with a minimal increase in the baseline resistance and excellent recovery of electrical properties upon relaxation. The electrode displays excellent strain‐rate stability and is capable of being stretched at a strain rate of 1000% s?1 with only a small increase in resistance. The electrode also displays excellent electrical stability. Applications of the composite electrode include highly stretchable soft capacitors and energy generators. The capacitance change along with stretching could be either linear for sensor purposes or superlinear for improved energy gains as an energy harvester. 相似文献
629.
Mesh‐Shaped Nanopatterning of Pt Counter Electrodes for Dye‐Sensitized Solar Cells with Enhanced Light Harvesting
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A facile process to produce large‐area platinum (Pt) counter electrode platforms with well‐arrayed, mesh‐shaped nanopatterns using commercially available TiO2 paste and poly(dimethyl siloxane) (PDMS) nanostamps is presented. The process involves mesh‐shaped (200 nm × 200 nm) nanopatterning of a TiO2 scaffold onto a fluorine‐doped tin oxide (FTO) substrate, followed by Pt sputtering. The structure and morphology of the counter electrodes are characterized by a field emission scanning electron microscope (FE‐SEM) and an atomic force microscope (AFM). Solid‐state dye‐sensitized solar cells (ssDSSCs) fabricated with these mesh‐shaped Pt counter electrodes showed an efficiency of 7.0%. This is one of the highest efficiencies observed for N719 dye and is much higher than that of devices with non‐patterned, thermally deposited electrodes (5.4%) or non‐patterned, sputtering deposited electrodes (5.7%). This improvement is attributed to enhanced light harvesting and a greater surface area and has been confirmed by incident photon‐to current efficiency (IPCE), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV) measurements. 相似文献
630.